Estudo da estabilidade térmica de complexos organometálicos derivados do 2-|2-hidroxifenil|benzoxazol

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Systems chemo-biology analysis of DNA damage response and cell cycle effects induced by coal exposure

Cell cycle alterations are among the principle hallmarks of cancer. Consequently, the study of cell cycle regulators has emerged as an important topic in cancer research, particularly in relation to environmental exposure. Particulate matter and coal dust around coal mines have the potential to induce cell cycle alterations. Therefore, in the present study, we performed chemical analyses to identify the main compounds present in two mineral coal samples from Colombian mines and performed systems chemo-biology analysis to elucidate the interactions between these chemical compounds and proteins associated with the cell cycle. Our results highlight the role of oxidative stress generated by the exposure to the residues of coal extraction, such as major inorganic oxides (MIOs), inorganic elements (IEs) and polycyclic aromatic hydrocarbons (PAH) on DNA damage and alterations in the progression of the cell cycle (blockage and/or delay), as well as structural dysfunction in several proteins. In particular, IEs such as Cr, Ni, and S and PAHs such as benzo[a]pyrene may have influential roles in the regulation of the cell cycle through DNA damage and oxidative stress. In this process, cyclins, cyclin-dependent kinases, zinc finger proteins such as TP53, and protein kinases may play a central role

AVALIAÇÃO ANTIMICROBIANA DO EXTRATO ETANÓLICO DA POLPA DE PEQUI (Caryocar coriaceum Wittm.) CONTRA MICRORGANISMOS PATOGÊNICOS

Microbial resistance to antimicrobials has been a growing threat to the effective treatment of an ever-increasing range of infections caused by bacteria and fungi; the reduced sensitivity of antibacterials and antifungals is making it difficult for many patients to treat. Pequi, in addition to cooking, is used for therapeutic purposes as an anti-inflammatory and antimicrobial in combating emergencies. However, little is known about the antimicrobial effects of its pulp and its phytochemical composition. The present work aimed to investigate the antimicrobial action of the ethanolic extract of the pulp of C. coriaceum Wittm. against two bacterial and one fungal strains of interest to oral health. The extract of the fruit of C. coriaceum was administered by experiment using a Soxhlet extractor and the antimicrobial activity was evaluated by the disk-agar diffusion technique on Enterococcus faecalis, Streptococcus pyogenes and Candida albicans. Yeast C. albicans was inhibited at the highest concentration, 25.2 mg. mL-1 of extract, with the average of halos being 10 mm; for the bacterium E. faecalis, the concentration level was verified at concentrations of 25.2 and 2.52 mg. mL-1 with halos of 8 and 6 mm, respectively. The results verified that the ethanolic extract of the C. coriaceum pulp presents indicative of antifungal and antibacterial activity. Further studies are suggested regarding its potential antimicrobial effect.A resistência microbiana aos antimicrobianos tem sido uma ameaça crescente para o tratamento efetivo de uma gama cada vez maior de infecções causadas por bactérias e fungos; a sensibilidade reduzida de antibacterianos e antifúngicos, vem tornando o tratamento de muitos pacientes difícil. O Pequi, além da qualinária, é utilizado para fins terapêuticos como um anti-inflamatório e antimicrobiano no combate a infecções. No entanto, sabe-se pouco sobre os efeitos antimicrobianos da sua polpa e sobre a sua composição fitoquímica. O presente trabalho visou investigar, a ação antimicrobiana do extrato etanólico da polpa de C. coriaceum Wittm. frente a duas cepas bacterianas e uma fúngica de interesse à saúde bucal. O extrato do fruto de C. coriaceum foi obtido por extração empregando extrator Soxhlet e a atividade antimicrobiana foi avaliada pela técnica difusão em disco-ágar, sobre Enterococcus faecalis, Streptococcus pyogenes e Candida albicans. A levedura C. albicans foi inibida na concentração mais elevada, 25,2 mg.mL-1 de extrato, com a média dos halos ficando em 10 mm; para a bactéria E. faecalis foi verificada inibição leve nas concentrações de 25,2 e 2,52 mg.mL-1 com halos de 8 e 6 mm, respectivamente. Os resultados verificaram que o extrato etanólico da polpa C. coriaceum apresenta indicativo de atividade antifúngica e antibacteriana. Sugerem-se mais estudos quanto ao seu potencial efeito antimicrobiano

Purificação do óleo de fritura e aplicação do óleo refinado na Síntese de Factis

O objetivo deste trabalho foi o de purificar óleo/gordura provenientes do processo de fritura (OGF)para reaproveitamento na obtenção de factics, um aditivo usado na indústria da borracha. Este processoagrega valor ao material e evita o descarte do OGF com conseqüente poluição ambiental. Diferentesprocedimentos de purificação foram avaliados e foi possível concluir que com 8% de terra natural e semvácuo obtém-se OGF purificado com propriedades físicas e químicas semelhantes ao óleo vegetal refinadocomercial (OVR). Também foi possível estabelecer condições das reações de polimerização e vulcanizaçãopara obtenção de factis a partir do OVR. O material apresentou especificações semelhantes a um produtoatualmente comercializado.Palavras-chave: óleo, gordura, fritura, factis, borracha

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe

Estudo computacional da fotofísica do 4-(2'-benzoxazolil)-2,5-diidroxibenzoato de etila

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Modelagem e preparação de bis-benzazolas para o desenvolvimento de dispositivos ópticos

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Síntese de novas Probes fluorescentes com potencial uso na marcação de proteínas

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Síntese de novas Probes fluorescentes com potencial uso na marcação de proteínas

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Estudo da estabilidade térmica de complexos organometálicos derivados do 2-|2-hidroxifenil|benzoxazol

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