Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy

Bile acids (BAs), endogenous acidic steroids synthetized from cholesterol in the liver, play a key role in the gut–liver axis physiopathology, including in hepatotoxicity, intestinal inflammatory processes, and cholesterol homeostasis. Faecal Oxo-BAs, relatively stable intermediates of oxidation/epimerization reactions of the BA hydroxyls, could be relevant to investigating the crosstalk in the liver–gut axis and the relationship between diseases and alterations in microbiota composition. A paucity of information currently exists on faecal BA profiles in dogs with and without chronic inflammatory enteropathy (CIE). Comprehensive assessment of 31 molecules among faecal BAs and related microbiota metabolites was conducted with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Odds ratios (ORs) for associations of BAs with CIE were estimated using logistic regression. Principal component analysis was performed to find differences between the control and pathological dogs. Higher levels of primary BAs and muricholic acids, and lower levels of secondary BAs were found in pathological dogs. Higher concentrations in faecal oxo-metabolites were associated with the absence of CIE (OR < 1). This study shows a marked difference in faecal BA profiles between dogs with and without CIE. Further research will be needed to better understand the role of oxo-BAs and muricholic acids in CIE dogs

FIRI - a Far-Infrared Interferometer

Half of the energy ever emitted by stars and accreting objects comes to us in the FIR waveband and has yet to be properly explored. We propose a powerful Far-InfraRed Interferometer mission, FIRI, to carry out high-resolution imaging spectroscopy in the FIR. This key observational capability is essential to reveal how gas and dust evolve into stars and planets, how the first luminous objects in the Universe ignited, how galaxies formed, and when super-massive black holes grew. FIRI will disentangle the cosmic histories of star formation and accretion onto black holes and will trace the assembly and evolution of quiescent galaxies like our Milky Way. Perhaps most importantly, FIRI will observe all stages of planetary system formation and recognise Earth-like planets that may harbour life, via its ability to image the dust structures in planetary systems. It will thus address directly questions fundamental to our understanding of how the Universe has developed and evolved - the very questions posed by ESA's Cosmic Vision.Comment: Proposal developed by a large team of astronomers from Europe, USA and Canada and submitted to the European Space Agency as part of "Cosmic Vision 2015-2025

Herschel observations of deuterated water towards Sgr B2(M)

Observations of HDO are an important complement for studies of water, because they give strong constraints on the formation processes -- grain surfaces versus energetic process in the gas phase, e.g. in shocks. The HIFI observations of multiple transitions of HDO in Sgr~B2(M) presented here allow the determination of the HDO abundance throughout the envelope, which has not been possible before with ground-based observations only. The abundance structure has been modeled with the spherical Monte Carlo radiative transfer code RATRAN, which also takes radiative pumping by continuum emission from dust into account. The modeling reveals that the abundance of HDO rises steeply with temperature from a low abundance ($2.5\times 10^{-11}$) in the outer envelope at temperatures below 100~K through a medium abundance ($1.5\times 10^{-9}$) in the inner envelope/outer core, at temperatures between 100 and 200~K, and finally a high abundance ($3.5\times 10^{-9}$) at temperatures above 200~K in the hot core.Comment: A&A HIFI special issue, accepte

Systematic Molecular Differentiation in Starless Cores

(Abridged) We present evidence that low-mass starless cores, the simplest units of star formation, are systematically differentiated in their chemical composition. Molecules including CO and CS almost vanish near the core centers, where the abundance decreases by one or two orders of magnitude. At the same time, N2H+ has a constant abundance, and the fraction of NH3 increases toward the core center. Our conclusions are based on a study of 5 mostly-round starless cores (L1498, L1495, L1400K, L1517B, and L1544), which we have mappedin C18O(1-0), C17O(1-0), CS(2-1), C34S(2-1), N2H+(1-0), NH3(1,1) and (2,2), and the 1.2 mm continuum. For each core we have built a model that fits simultaneously the radial profile of all observed emission and the central spectrum for the molecular lines. The observed abundance drops of CO and CS are naturally explained by the depletion of these molecules onto dust grains at densities of 2-6 10^4 cm-3. N2H+ seems unaffected by this process up to densities of several 10^5, while the NH3 abundance may be enhanced by reactions triggered by the disappearance of CO from the gas phase. With the help of our models, we show that chemical differentiation automatically explains the discrepancy between the sizes of CS and NH3 maps, a problem which has remained unexplained for more than a decade. Our models, in addition, show that a combination of radiative transfer effects can give rise to the previously observed discrepancy in the linewidth of these two tracers. Although this discrepancy has been traditionally interpreted as resulting from a systematic increase of the turbulent linewidth with radius, our models show that it can arise in conditions of constant gas turbulence.Comment: 25 pages, 9 figures, accepted by Ap

Herschel observations of EXtraordinary Sources: Analysis of the full Herschel/HIFI molecular line survey of Sagittarius B2(N)

A sensitive broadband molecular line survey of the Sagittarius B2(N) star-forming region has been obtained with the HIFI instrument on the Herschel Space Observatory, offering the first high-spectral resolution look at this well-studied source in a wavelength region largely inaccessible from the ground (625-157 um). From the roughly 8,000 spectral features in the survey, a total of 72 isotopologues arising from 44 different molecules have been identified, ranging from light hydrides to complex organics, and arising from a variety of environments from cold and diffuse to hot and dense gas. We present an LTE model to the spectral signatures of each molecule, constraining the source sizes for hot core species with complementary SMA interferometric observations, and assuming that molecules with related functional group composition are cospatial. For each molecule, a single model is given to fit all of the emission and absorption features of that species across the entire 480-1910 GHz spectral range, accounting for multiple temperature and velocity components when needed to describe the spectrum. As with other HIFI surveys toward massive star forming regions, methanol is found to contribute more integrated line intensity to the spectrum than any other species. We discuss the molecular abundances derived for the hot core, where the local thermodynamic equilibrium approximation is generally found to describe the spectrum well, in comparison to abundances derived for the same molecules in the Orion KL region from a similar HIFI survey.Comment: Accepted to ApJ. 64 pages, 14 figures. Truncated abstrac

Herschel observations of EXtra-Ordinary Sources: The Terahertz spectrum of Orion KL seen at high spectral resolution

We present the first high spectral resolution observations of Orion KL in the frequency ranges 1573.4 - 1702.8 GHz (band 6b) and 1788.4 - 1906.8 GHz (band 7b) obtained using the HIFI instrument on board the Herschel Space Observatory. We characterize the main emission lines found in the spectrum, which primarily arise from a range of components associated with Orion KL including the hot core, but also see widespread emission from components associated with molecular outflows traced by H2O, SO2, and OH. We find that the density of observed emission lines is significantly diminished in these bands compared to lower frequency Herschel/HIFI bands.Comment: Accepted for publication in the Herschel HIFI special issue of Astronomy and Astrophysics Letters, 5 pages, 3 figure

Herschel observations of extra-ordinary sources: Detection of Hydrogen Fluoride in absorption towards Orion~KL

We report a detection of the fundamental rotational transition of hydrogen fluoride in absorption towards Orion KL using Herschel/HIFI. After the removal of contaminating features associated with common molecules ("weeds"), the HF spectrum shows a P-Cygni profile, with weak redshifted emission and strong blue-shifted absorption, associated with the low-velocity molecular outflow. We derive an estimate of 2.9 x 10^13 cm^-2 for the HF column density responsible for the broad absorption component. Using our best estimate of the H2 column density within the low-velocity molecular outflow, we obtain a lower limit of ~1.6 x 10^-10 for the HF abundance relative to hydrogen nuclei, corresponding to 0.6% of the solar abundance of fluorine. This value is close to that inferred from previous ISO observations of HF J=2--1 absorption towards Sgr B2, but is in sharp contrast to the lower limit of 6 x 10^-9 derived by Neufeld et al. (2010) for cold, foreground clouds on the line of sight towards G10.6-0.4.Comment: 5 pages, 3 figures, paper to be published in the Herschel special issue of A&A letter

Herschel observations of extra-ordinary sources: Detecting spiral arm clouds by CH absorption lines

We have observed CH absorption lines ($J=3/2, N=1 \leftarrow J=1/2, N=1$) against the continuum source Sgr~B2(M) using the \textit{Herschel}/HIFI instrument. With the high spectral resolution and wide velocity coverage provided by HIFI, 31 CH absorption features with different radial velocities and line widths are detected and identified. The narrower line width and lower column density clouds show `spiral arm' cloud characteristics, while the absorption component with the broadest line width and highest column density corresponds to the gas from the Sgr~B2 envelope. The observations show that each `spiral arm' harbors multiple velocity components, indicating that the clouds are not uniform and that they have internal structure. This line-of-sight through almost the entire Galaxy offers unique possibilities to study the basic chemistry of simple molecules in diffuse clouds, as a variety of different cloud classes are sampled simultaneously. We find that the linear relationship between CH and H$_2$ column densities found at lower $A_V$ by UV observations does not continue into the range of higher visual extinction. There, the curve flattens, which probably means that CH is depleted in the denser cores of these clouds.Comment: Accepted for publication in A&A, HIFI Special Issu

Deuterated water in the solar-type protostars NGC 1333 IRAS 4A and IRAS 4B

Aims. The aim of this paper is to study deuterated water in the solar-type protostars NGC1333 IRAS4A and IRAS4B, to compare their HDO abundance distribution with other star-forming regions, and to constrain their HDO/H2O ratios. Methods. Using the Herschel/HIFI instrument as well as ground-based telescopes, we observed several HDO lines covering a large excitation range (Eup/k=22-168 K) towards these protostars and an outflow position. Non-LTE radiative transfer codes were then used to determine the HDO abundance profiles in these sources. Results. The HDO fundamental line profiles show a very broad component, tracing the molecular outflows, in addition to a narrower emission component and a narrow absorbing component. In the protostellar envelope of NGC1333 IRAS4A, the HDO inner (T>100 K) and outer (T<100 K) abundances with respect to H2 are estimated at 7.5x10^{-9} and 1.2x10^{-11}, respectively, whereas, in NGC1333 IRAS4B, they are 1.0x10^{-8} and 1.2x10^{-10}, respectively. Similarly to the low-mass protostar IRAS16293-2422, an absorbing outer layer with an enhanced abundance of deuterated water is required to reproduce the absorbing components seen in the fundamental lines at 465 and 894 GHz in both sources. This water-rich layer is probably extended enough to encompass the two sources as well as parts of the outflows. In the outflows emanating from NGC1333 IRAS4A, the HDO column density is estimated at about (2-4)x10^{13} cm^{-2}, leading to an abundance of about (0.7-1.9)x10^{-9}. An HDO/H2O ratio between 7x10^{-4} and 9x10^{-2} is derived in the outflows. In the warm inner regions of these two sources, we estimate the HDO/H2O ratios at about 1x10^{-4}-4x10^{-3}. This ratio seems higher (a few %) in the cold envelope of IRAS4A, whose possible origin is discussed in relation to formation processes of HDO and H2O.Comment: 16 pages, 13 figure