A light-activated antimicrobial surface is active against bacterial, viral and fungal organisms

Evidence has shown that environmental surfaces play an important role in the transmission of nosocomial pathogens. Deploying antimicrobial surfaces in hospital wards could reduce the role environmental surfaces play as reservoirs for pathogens. Herein we show a significant reduction in viable counts of Staphylococcus epidermidis, Saccharomyces cerevisiae, and MS2 Bacteriophage after light treatment of a medical grade silicone incorporating crystal violet, methylene blue and 2 nm gold nanoparticles. Furthermore, a migration assay demonstrated that in the presence of light, growth of the fungus-like organism Pythium ultimum and the filamentous fungus Botrytis cinerea was inhibited. Atomic Force Microscopy showed significant alterations to the surface of S. epidermidis, and electron microscopy showed cellular aggregates connected by discrete surface linkages. We have therefore demonstrated that the embedded surface has a broad antimicrobial activity under white light and that the surface treatment causes bacterial envelope damage and cell aggregation

Exponential unitary integrators for nonseparable quantum Hamiltonians

Quantum Hamiltonians containing nonseparable products of non-commuting operators, such as $\hat{\bf x}^m \hat{\bf p}^n$, are problematic for numerical studies using split-operator techniques since such products cannot be represented as a sum of separable terms, such as $T(\hat{\bf p}) + V(\hat{\bf x})$. In the case of classical physics, Chin [Phys. Rev. E $\bf 80$, 037701 (2009)] developed a procedure to approximately represent nonseparable terms in terms of separable ones. We extend Chin's idea to quantum systems. We demonstrate our findings by numerically evolving the Wigner distribution of a Kerr-type oscillator whose Hamiltonian contains the nonseparable term $\hat{\bf x}^2 \hat{\bf p}^2 + \hat{\bf p}^2 \hat{\bf x}^2$. The general applicability of Chin's approach to any Hamiltonian of polynomial form is proven.Comment: Fixed typos and extended discussion (6 pages, 1 figure

Applied Methods to Assess the Antimicrobial Activity of Metallic-Based Nanoparticles

With the rise of antibiotic resistance, the drive to discover novel antimicrobial substances and standard testing methods with the aim of controlling transmissive diseases are substantially high. In healthcare sectors and industries, although methods for testing antibiotics and other aqueous-based reagents are well established, methods for testing nanomaterials, non-polar and other particle-based suspensions are still debatable. Hence, utilities of ISO standard validations of such substances have been recalled where corrective actions had to be taken. This paper reports a serial analysis obtained from testing the antimicrobial activities of 10 metallic-based nanomaterials against 10 different pathogens using five different in vitro assays, where the technique, limitation and robustness of each method were evaluated. To confirm antimicrobial activities of metallic-based nanomaterial suspensions, it was found that at least two methods must be used, one being the agar well diffusion method, which was found to be the most reliable method. The agar well diffusion method provided not only information on antimicrobial efficacy through the size of the inhibitory zones, but it also identified antimicrobial ions and synergistic effects released by the test materials. To ascertain the effective inhibitory concentration of nanoparticles, the resazurin broth dilution method is recommended, as MIC can be determined visually without utilising any equipment. This method also overcomes the limit of detection (LoD) and absorbance interference issues, which are often found in the overexpression of cell debris and nanoparticles or quantum dots with optical profiles. In this study, bimetallic AgCu was found to be the most effective antimicrobial nanoparticle tested against across the bacterial (MIC 7 µg/mL) and fungal (MIC 62.5 µg/mL) species

Autopneumonectomy with Compensatory Lung Growth

A 23-year-old female immigrant from Ethiopia presented with a history of hemoptysis and an abnormal chest x-ray. A computed tomography scan showed that her left lung was greatly shrunken and her right lung was very large but structurally normal. She had a history of multiple respiratory infections as a young child but had been well since the age of five years. Her lung function was within normal limits except for an increased residual volume. It is very likely that her left lung was destroyed early in childhood and that her right lung underwent compensatory growth. She did not show airways obstruction, which is usually seen when compensatory lung growth occurs after surgical removal of lung tissue; this may indicate that, in those cases, the surgery compromised airway function.Peer Reviewe

Elemental Analysis and Phenolic Profiles of Selected Italian Wines

The study of the chemical composition of wines is nowadays a topic of great interest because of the importance of this market, especially in Italy, and also considering the numerous cases of falsification of famous and very expensive wines. The present paper focused on the analysis of metals and polyphenols in Italian wines belonging to different provenance and types. At this purpose 20 elements were quantified by inductively coupled plasma optical emission spectrometry (ICP-OES) and ICP mass spectrometry (ICP-MS). Regarding polyphenols, a total of 32 were quantified, among 6 were anthocyanins. Furthermore, in 4 samples (1 rosè and 3 red wines) 42 anthocyanins and related compounds were identified by ultra-high performance liquid chromatography (UHPLC)-Orbitrap MS technique (among these, 6 were also quantified). Non-anthocyanins were determined using UHPLC coupled with a diode array detector and triple-quadrupole mass spectrometer (UHPLC–DAD-QqQ-MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were measured using spectrophotometric methods. The results obtained by elemental techniques were submitted to principal components analysis (PCA) allowing to get information on both geographical and botanical origin of the examined wine samples. Some polyphenols have been detected in higher concentrations only in a certain type of wine, as for example in the case of Grechetto wine. Most of the identified anthocyanin derivatives (pyranoanthocyanins) are formed during the aging of wine by reaction with the other wine components

999 Conference on Electrical [nsulation and Dielectric Phenomena Analytical Solution and Scaled Model of a Unipolar HVDC Transn1ission Line

Abstract Existing analytical solutions for the ionized lield of a unipolar HVDC transmission line are reviewed. The analytical relations indicate that the corona current is directly proportional to the square of the corona onset field strength if the ratio of the applied voltage to the corona onset value and that of the conductor height to its radius remain fixed. This is confirmed by numerical results. It is pointed out that this observation makes it possible to derive the basis of a scaled model to evaluate the corona current and hence the resulting power loss of a full-scale unipolar de line

Helium exhaust experiments on JET with Type I ELMs in H-mode and with type III ELMs in ITB discharges

An analysis of helium exhaust experiments on JET in the MkII-GB divertor configuration is presented. Helium is pumped by applying an argon frost layer on the divertor cryo pump. Measurement of the helium retention time, tau(He)(*),, is performed in two ways: by the introduction of helium in gas puffs and measurement of the subsequent decay time constant of the helium content, tau(He)(d*); and by helium beam injection and measurement of the helium replacement time, tau(He)(r*). In ELMy H-mode, with plasma configuration optimized for pumping, tau(He)(d*) approximate to 7.2 x tau(E)(th) is achieved, where tau(E)(th) is the thermal energy replacement time. For quasi-steady internal transport barrier (ITB) discharges, the achieved tau(He)(r*) approximate to 4.1 x tau(E)(th) is significantly lower. The achieved helium recycling coefficient, confirmed by an independent measurement to be R-eff approximate to 0.91, is the same in both scenarios. None of the discharges are dominated by core confinement. The difference in tau(He)(*)/tau(E)(th) is instead due to the confinement properties of the edge plasma, which is characterized by Type I ELMs for the H-mode discharges studied, and Type III ELMs for the quasi-steady ITB discharges. This difference is quantified by an independent measurement of the ratio of the helium replacement time with a helium edge source to the energy confinement time

Modelling of the effect of ELMs on fuel retention at the bulk W divertor of JET

Effect of ELMs on fuel retention at the bulk W target of JET ITER-Like Wall was studied with multi-scale calculations. Plasma input parameters were taken from ELMy H-mode plasma experiment. The energetic intra-ELM fuel particles get implanted and create near-surface defects up to depths of few tens of nm, which act as the main fuel trapping sites during ELMs. Clustering of implantation-induced vacancies were found to take place. The incoming flux of inter-ELM plasma particles increases the different filling levels of trapped fuel in defects. The temperature increase of the W target during the pulse increases the fuel detrapping rate. The inter-ELM fuel particle flux refills the partially emptied trapping sites and fills new sites. This leads to a competing effect on the retention and release rates of the implanted particles. At high temperatures the main retention appeared in larger vacancy clusters due to increased clustering rate

On the mechanisms governing gas penetration into a tokamak plasma during a massive gas injection

A new 1D radial fluid code, IMAGINE, is used to simulate the penetration of gas into a tokamak plasma during a massive gas injection (MGI). The main result is that the gas is in general strongly braked as it reaches the plasma, due to mechanisms related to charge exchange and (to a smaller extent) recombination. As a result, only a fraction of the gas penetrates into the plasma. Also, a shock wave is created in the gas which propagates away from the plasma, braking and compressing the incoming gas. Simulation results are quantitatively consistent, at least in terms of orders of magnitude, with experimental data for a D 2 MGI into a JET Ohmic plasma. Simulations of MGI into the background plasma surrounding a runaway electron beam show that if the background electron density is too high, the gas may not penetrate, suggesting a possible explanation for the recent results of Reux et al in JET (2015 Nucl. Fusion 55 093013)