Rapid analysis of fluoxetine and its metabolite in plasma by LC-MS with column-switching approach

Abstract.: A rapid and sensitive method was developed for the simultaneous determination of fluoxetine and its primary metabolite, norfluoxetine, in plasma. It was based on a column-switching approach with a precolumn packed with large size particles coupled with a liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS). After a simple centrifugation, plasma samples were directly injected onto the precolumn. The endogenous material was excluded thanks to a high flow rate while analytes were retained by hydrophobic interactions. Afterwards, the target compounds were eluted in back flush mode to an octadecyl analytical column and detected by ESI-MS. The overall analysis time per sample, from plasma sample preparation to data acquisition, was achieved in less than 4min. Method performances were evaluated. The method showed good linearity in the range of 25-1000ngmL−1 with a determination coefficient higher than 0.99. Limits of quantification were estimated at 25ngmL−1 for fluoxetine and norfluoxetine. Moreover, method precision was better than 6% in the studied concentration range. These results demonstrated that the method could be used to quantify target compounds. Finally, the developed assay proved to be suitable for the simultaneous analysis of fluoxetine and its metabolite in real plasma sample

Electrical characterization of the soft breakdown failure mode in MgO layers

The soft breakdown (SBD) failure mode in 20 nm thick MgO dielectric layers grown on Si substrates was investigated. We show that during a constant voltage stress, charge trapping and progressive breakdown coexist, and that the degradation dynamics is captured by a power-law time dependence. We also show that the SBD current-voltage (I-V) characteristics follow the power-law model I = aVb typical of this conduction mechanism but in a wider voltage window than the one reported in the past for SiO2. The relationship between the magnitude of the current and the normalized differential conductance was analyzed

Multi-technique characterisation of MOVPE-grown GaAs on Si

The heterogeneous integration of III-V materials on a Si CMOS platform offers tremendous prospects for future high speed and low power logic applications. That said this integration generates immense scientific and technological challenges. In this work multi-technique characterisation is used to investigate properties of GaAs layers grown by Metal-Organic Vapour Phase Epitaxy (MOVPE) on Si substrates - (100) with 4⁰ offset towards - under various growth conditions. This being a crucial first step towards the production of III-V template layers with a relatively lower density of defects for selective epitaxial overgrowth of device quality material. The optical and structural properties of heteroepitaxial GaAs are first investigated by micro-Raman spectroscopy and photoluminescence and reflectance measurements. High-resolution X-ray diffraction (HR-XRD) is used to investigate structural properties. Advanced XRD techniques, including double-axis diffraction and X-ray crystallographic mapping are used to evaluate degrees of relaxation and distribution of the grain orientations in the epilayers, respectively. Results obtained from the different methodologies are compared in an attempt to understand growth kinetics of the materials system. The GaAs overlayer grown with annealing at 735⁰C following As predeposition at 500⁰C shows the best crystallinity. Close inspection confirms the growth of epitaxial GaAs preferentially oriented along (100) embedded in a highly-textured polycrystalline structure

Case Report of Cutaneous Large B-cell leg-type Lymphoma in Young Woman

Cutaneous diffuse B-cell lymphoma leg type is a rare entity, it represents approximately 5% of primary cutaneous lymphomas. It has a poor prognosis. We report case of t 58 years old patient with a cutaneous B cell lymphoma leg type with dual location at the upper and lower limbs and bone lysis. Histological examination of the skin biopsy showed diffuse lymphomatous proliferation dissecting collagen, made of large cells with abundant cytoplasm moderately ill-defined, ovoid nuclei and with a discreet nuclear irregularity. The immunohistochemical study revealed lymphoma cells phenotype B with a homogeneous expression of CD 20, a homogeneous expression of CD 3, CD 43 positive. Bcl 2 was positive, asthe Bcl 6 and Mum 1. CD 5, CD10, MPO, CD 34, and Tdt were negative. The Ki 67 was positivein90%. The staging showed extensive osteolysis of the lower end of the humerus and the ulna. The patient received 2 courses RCHOP, with faillure of treatment, and then she received one course of RDHAP before dying. The particularity of this observation consists in the age; our patient was younger than the average, and the double localization of lymphoma in both arm and leg, with osteolysis. We discuss also the difficulties of treatment of this disease

Study of the effect of chromium on the germination parameters of Fenugreek (Trigonella foenum-gracium L.) and Lens (Lens culinaris)

Received: September 21st, 2022 ; Accepted: January 2nd, 2023 ; Published: February 8th, 2023 ; Correspondence: [email protected] contamination by heavy metals is a global environmental problem. This contamination affects agricultural crops in the area concerned. In the present study, chromium, which is a heavy metal, is evaluated for its diverse effects on seed germination and lateral growth of fenugreek and lens seeds. A chromium solution was prepared at increasing concentrations: 0, 0.02, 0.04, 0.06, 0.08, 0.1, and 0.2 mg L-1 for the addition of germinating seeds in petri dishes for ten days. After two days, the germination rate is calculated. For the following days the length of radicle, stem, and number of leaves are measured. The germination rate of fenugreek varies between 100 and 73.33% for the control and 0.02 mg L-1 of chromium respectively. However, the germination rate of the lens varies between 100% for the control and 90% for the 0.02 mg L-1 . The elongation of fenugreek radicle with chromium solutions shows a significant effect. However, there is no significant difference in the lens at the different concentrations. For the growth of the fenugreek stalk, it is noticed that the concentration 0.02 shows a length of 2.83 cm compared to their control which is 2.30 cm. Consequently, chromium at 0.02 mg L-1 stimulates growth, but at 0.2 mg L-1 , it inhibits it. For lens the length of the stems shows also a significant difference compared to their control. So the effect of chromium on germination parameters depends on their concentrations, as well as on the seed response itself. For our research the response of fenugreek compared to the lens at the same concentrations is different

Seismic risk in the city of Al Hoceima (north of Morocco) using the vulnerability index method, applied in Risk-UE project

The final publication is available at Springer via http://dx.doi.org/10.1007/s11069-016-2566-8Al Hoceima is one of the most seismic active regions in north of Morocco. It is demonstrated by the large seismic episodes reported in seismic catalogs and research studies. However, seismic risk is relatively high due to vulnerable buildings that are either old or don’t respect seismic standards. Our aim is to present a study about seismic risk and seismic scenarios for the city of Al Hoceima. The seismic vulnerability of the existing residential buildings was evaluated using the vulnerability index method (Risk-UE). It was chosen to be adapted and applied to the Moroccan constructions for its practicality and simple methodology. A visual inspection of 1102 buildings was carried out to assess the vulnerability factors. As for seismic hazard, it was evaluated in terms of macroseismic intensity for two scenarios (a deterministic and probabilistic scenario). The maps of seismic risk are represented by direct damage on buildings, damage to population and economic cost. According to the results, the main vulnerability index of the city is equal to 0.49 and the seismic risk is estimated as Slight (main damage grade equal to 0.9 for the deterministic scenario and 0.7 for the probabilistic scenario). However, Moderate to heavy damage is expected in areas located in the newer extensions, in both the east and west of the city. Important economic losses and damage to the population are expected in these areas as well. The maps elaborated can be a potential guide to the decision making in the field of seismic risk prevention and mitigation strategies in Al Hoceima.Peer ReviewedPostprint (author's final draft

Hashimoto’s thyroiditis and acute chest syndrome revealing sickle cell anemia in a 32 years female patient

Sickle cell anemia results from a single amino acid substitution in the gene encoding the 4-globin subunit. Polymerization of deoxygenated sickle hemoglobin leads to decreased deformability of red blood cells. Hashimoto's thyroiditis is a common thyroid disease now recognized as an autoimmune thyroid disorder, it is usually thought to be haemolytic autoimmune anemia. We report the case of a 32 years old women admitted for chest pain and haemolysis anemia in which Hashimoto's thyroiditis and sickle cell anemia were found. In our observation the patient is a young woman whose examination did not show signs of goitre but the analysis of thyroid function tests performed before an uto-immune hemolytic anemia (confirmed by a high level of unconjugated bilirubin and a Coombs test positive for IgG) has found thyroid stimulating hormone (TSH) and positive thyroid antibody at rates in excess of 4.5 times their normal value. In the same period, as the hemolytic anemia, and before the atypical chest pain and anguish they generated in the patient, the search for hemoglobinopathies was made despite the absence of a family history of haematological disease or painful attacks in childhood. Patient electrophoresis's led to research similar cases in the family. The mother was the first to be analyzed with ultimately diagnosed with sickle cell trait have previously been ignored. This case would be a form with few symptoms because the patient does not describe painful crises in childhood or adolescence

Vegetative Propagation of Argania spinosa (L.) Skeels Cuttings: Effects of Nutrient Solution

The effects of the mineral composition of nutrient solution (Hoagland and Arnon (HA), Quoirin and Lepoivre (QL), Murashige and Skoog (MS), and Woody Plant Medium (WPM)), cutting type (softwood, semi-hardwood and hardwood) and cutting position (basal, medial, and apical) on sprouting and rooting performance of Argania spinosa cuttings were investigated. According to the results, the nutrient solution, cutting type and cutting position had an effect on the sprouting and adventitious rooting ability of A. spinosa cuttings. The leafy semi-hardwood cuttings taking from the basal positions and irrigated with Hoagland solution performed best and produced the highest number of roots (44.63), root length (28.86 cm), and had the highest rooting and survival percentage (63.81% and 96.09%, respectively). The nutrient solution applications caused a notable increase in sprouting and rooting potential of the argan tree. The highest values were recorded for HA and QL, while the MS and WPM gave the poorest result and the greatest mortality rate of cuttings. The cuttings type had also a pronounced effect on vegetative propagation of A. spinosa. The leafy semi-hardwood cuttings performed better than the leafy softwood cuttings, whereas leafless hardwood cuttings were completely unable to sprout and root even when treated with nutrient solutions. Thus, vegetative propagation of A. spinosa can best be achieved using basal leafy semi-hardwood cuttings irrigated with Hoagland nutrient solution

Augmentation of Bone Tunnel Healing in Anterior Cruciate Ligament Grafts: Application of Calcium Phosphates and Other Materials

Bone tunnel healing is an important consideration after anterior cruciate ligament (ACL) replacement surgery. Recently, a variety of materials have been proposed for improving this healing process, including autologous bone tissue, cells, artificial proteins, and calcium salts. Amongst these materials are calcium phosphates (CaPs), which are known for their biocompatibility and are widely commercially available. As with the majority of the materials investigated, CaPs have been shown to advance the healing of bone tunnel tissue in animal studies. Mechanical testing shows fixation strengths to be improved, particularly by the application of CaP-based cement in the bone tunnel. Significantly, CaP-based cements have been shown to produce improvements comparable to those induced by potentially more complex treatments such as biologics (including fibronectin and chitin) and cultured cells. Further investigation of CaP-based treatment in the bone tunnels during ACL replacement is therefore warranted in order to establish what improvements in healing and resulting clinical benefits may be achieved through its application

Defect study of GaInP/GaAs based heterojunction bipolar transistor emitter layer

Defects in the emitter region of Ga0.51In0.49P/GaAs heterojunction bipolar transistors (HBTs) were investigated by means of deep-level transient spectroscopy. Both annealed (635 degreesC, 5 min) and as grown metalorganic chemical vapor deposition epitaxial wafers were investigated in this study, with an electron trap observed in the HBT emitter space-charge region from both wafers. The deep-level activation energy was determined to be 0.87+/-0.05 eV below the conduction band, the capture cross section 3x10(-14) cm(2) and the defect density of the order of 10(14) cm(-3). This defect was also found to be localized at the emitter-base interface