Common bean (Phaseolus vulgaris L.) landraces in Catalonia, a Mesoamerican germplasm hotspot to be preserved

[EN] Several landraces of common bean with high organoleptic value have resisted the rapid expansion of improved cultivars in Catalonia, in north-eastern Spain. To establish strategies for their preservation and use, we employed RAPD and AFLP markers to investigate the genetic variability within 15 landraces and to identify their original gene pools. A higher percentage of Mesoamerican landraces was found in Catalonia (40%) than in the rest of the Iberian Peninsula, or in Europe (approx. 20%). This is probably due to the exclusion of Catalonia from early trade with the American colonies and stronger commercial links with the Caribbean during the nineteenth century. Our results confirm that Catalan consumers prefer white-seeded common bean varieties of Mesoamerican origin. The landrace ‘Castellfollit del Boix’ showed 69.6% polymorphic RAPD primers, with 53.2% polymorphic bands, while at the other extreme ‘Tavella Brisa’ showed 47.8% polymorphic primers with 25.3% polymorphic bands. An AFLP approach yielded similar results. The high genetic variability found in ‘Castellfollit del Boix’, one of the landraces most threatened, suggests a considerable amount of introgression from improved inbreds.This work was supported by a research grant from CICYT (AGL0035-01).Sanchez, E.; Sifres Cuerda, AG.; Casañas, F.; Nuez Viñals, F. (2007). Common bean (Phaseolus vulgaris L.) landraces in Catalonia, a Mesoamerican germplasm hotspot to be preserved. Journal of Horticultural Science. 82(4):529-534. https://doi.org/10.1080/14620316.2007.11512269S529534824Rodrigo, A. P. (2000).Caracterización Morfoagronómica y Bioquímica del Germoplasma de la Judía Común (Phaseolus vulgarisL.) de España.Ph.D. Thesis, Universidad de Santiago de Compostela, Santiago, Spain. 251 pp

Sub-toxic ethanol exposure modulates gene expression and enzyme activity of antioxidant systems to provide neuroprotection in hippocampal HT22 cells

Ethanol is known to cause severe systemic damage often explained as secondary to oxidative stress. Brain is particularly vulnerable to ethanol-induced reactive oxygen species (ROS) because the high amounts of lipids, and because nerve cell membranes contain high amounts of peroxidable fatty acids. Usually these effects of ethanol are associated to high and/or chronic exposure to ethanol. However, as we show in this manuscript, a low and acute dose of ethanol trigger a completely different response in hippocampal cells. Thus, we have observed that 0.1% ethanol exposure to HT22 cells, a murine hippocampal-derived cell line, increases the transcriptional expression of different genes belonging to the classical, glutathione/glutaredoxin and thioredoxin/peroxiredoxin antioxidant systems, these including Sod1, Sod2, Gpx1, Gclc and Txnrd1. Paralleling these changes, enzyme activities of total superoxide dismutase (tSOD), catalase, total glutathione peroxidase (tGPx), glutathione-S-reductase (GSR) and total thioredoxin reductase (tTXNRD), were all increased, while the generation of thiobarbituric acid reactive substances (TBARS), as indicators of lipid peroxidation, and glutathione levels remained unaltered. Ethanol exposure did not affect cell viability or cell growing as assessed by real-time cell culture monitoring, indicating that low ethanol doses are not deleterious for hippocampal cells, but rather prevented glutamate-induced excitotoxicity. In summary, we conclude that sub-toxic exposure to ethanol may well be neuroprotective against oxidative insults in hippocampal cells