Characterization of the fecal microbiota in primary Sjögren syndrome patients and the relationship with intestinal permeability and inflammation

El síndrome de Sjögren (SS) es una enfermedad de tipo autoinmune con importante afectación ocular. Su etiología es aún desconocida con un numero de factores elevados como posibles generadores de la enfermedad. El cambio en la microbiota intestinal ha sido reportado como un factor diferencial entre los sujetos sanos y los sujetos con SS. Diferencias en la microbiota intestinal han sido reportadas en otras patologías sistémicas autoinmunes. Para no tener factores de confusión entre otras patologías sistémicas autoinmunes se ha realizado un estudio en pacientes estrictamente seleccionados y con ausencia de otras alteraciones inmunitarias, siendo seleccionados solo los pacientes con síndrome de Sjögren primario (SSP). Los objetivos de esta tesis doctoral han sido la caracterización de microbiota fecal en pacientes con SSP comparados con pacientes sanos, la determinación de parámetros inflamatorios en plasma de pacientes con SSP comparados con pacientes sanos y su relación con microbiota fecal, así como la determinación de permeabilidad intestinal a través de zonulina plasmática. En esta tesis doctoral se ha realizado un estudio de pacientes con SSP para poder establecer si los cambios en la microbiota intestinal son asociados a la presencia de esta enfermedad de forma aislada, o bien si en el SSP no existen diferencias con los sujetos sanos. De este modo podríamos centrar investigaciones posteriores en otras patologías autoinmunes como la artritis reumatoide (AR) y el lupus eritematosis sistémico (LES), entre otras. Algunos autores han estudiado patologías autoinmunes encontrando diferencias en la microbiota intestinal lo que nos ha llevado a profundizar en el estudio del SSP como entidad aislada sin otras enfermedades autoinmunes asociadas que nos lleven a posibles confusiones. Se han estudiado parámetros de inflamación y de permeabilidad intestinal para intentar establecer relaciones entre estos parámetros y los cambios de la microbiota intestinal, así como se ha intentado establecer o aportar información sobre la etiopatogenia de la enfermedad. Los resultados obtenidos muestran que la microbiota intestinal de los pacientes con SSP difieren tanto a nivel taxonómico como a nivel funcional con respecto a controles sanos. El perfil de microbiota intestinal de nuestros pacientes con SSP presento una menor diversidad y riqueza siendo el filo Bacteroidetes el grupo más representado. Se ha encontrado un aumento de la producción de citoquinas proinflamatorias y la disminución de la liberación de citoquinas antiinflamatorias (IL-10). Se necesitan mas estudios para comprender mejor la realidad del impacto de la disbiosis en el curso del SSP y para concebir estrategias preventivas o terapéuticas para contrarrestar la inflamación impulsada por el microbioma.Sjögren's syndrome (SS) is an autoimmune disease with significant ocular involvement. Its etiology is still unknown with a high number of factors as possible generators of the disease. The change in the gut microbiota has been reported as a differential factor between healthy subjects and subjects with SS. Differences in the intestinal microbiota have been reported in other autoimmune systemic pathologies. In order to avoid confounding factors between other autoimmune systemic pathologies, our study has been carried out in strictly selected patients and with the absence of other immune alterations, only selecting patients with primary Sjögren's syndrome (SSP). The objectives of this doctoral thesis have been the characterization of the fecal microbiota in patients with SSP compared to healthy patients, the determination of inflammatory parameters in plasma in SSP patients compared to healthy patients and their relationship with fecal microbiota as well as the determination of intestinal permeability through plasma zonulin. In this doctoral thesis, a study of patients with SSP has been carried out in order to establish if changes in the intestinal microbiota are associated with the presence of this disease in isolation, or if there are no differences in SSP with healthy subjects. In this way, we could focus further research on other autoimmune diseases such as rheumatoid arthritis (AR) and systemic lupus erythematosis (LES), among others. Some authors have studied autoimmune pathologies finding differences in the intestinal microbiota, which has led us to delve into the study of SSP as an isolated entity without other associated autoimmune diseases that lead us to possible confusion. Inflammation and intestinal permeability parameters have been studied to try to establish relationships between these parameters and changes in the intestinal microbiota, as well as attempts to establish or provide information on the etiopathogenesis of the disease. The results obtained show that the intestinal microbiota of patients with SSP differ both taxonomic level and functional level with respect to healthy controls. The intestinal microbiota profile of our patients with SSP presented less diversity and richness, the Bacteroidetes phylum was the most represented group An increase in the production of pro-inflammatory cytokines and a decrease in the release of anti-inflammatory cytokines (IL-10) have been found. More studies are needed to understand the impact of dysbiosis on the course of SSP and to devise preventive or therapeutic strategies to counter microbiome-driven inflammation

Formaciones de "Antirrhinum majus" en los territorios hispalenses

Stands of Antirrhinum majus in the Hispalense sectorPalabras clave. Asociación, sintaxonomia, corología, sur Península Ibérica, Echio boissieri-Antirrhinetum majii.Key words. Association, syntaxonomy, chorology, south of the Iberian Peninsula, Echio boissieri-Antirrhinetum majii

Study of the generation of microburrs in the process of drilling and its subsequent elimination by deburring, of tubes cylindrical aluminium AA6065-T9

Enllaç a la versió editorial: http://scitation.aip.org/dbt/dbt.jsp?KEY=APCPCS&Volume=1181&Issue=1Creation of microburrs drilling constitute a potencial danger because their detachment can modificate the correct operation of a mechanism. The burrs must be eliminated completely in the case of pistons brake, ensuring a high productivity and a low cost. In this work has been studied the mechanism for the creation of microburrs in thru holes drilled (phi2, 02 mm) of a aluminium alloy piston AA6065-T9 in order to various parameters such as the type of drill bit, feedrate, cutting speed and lubrication, imposing a minimum feedrate of 450 mm/min not to reduce the current production. Creation of microburrs drilling is inevitable and several deburring processes have been analyzed for their total elimination. After a few first essays, It has been determined that in order that the deburring are effective must be worked with minimal and known position burrr drillings.Peer ReviewedPostprint (author’s final draft

Cataract surgery astigmatism incisional management. Manual relaxing incision versus femtosecond laser-assisted arcuate keratotomy. A systematic review

Purpose: This systematic review aims to compare corneal astigmatism correction in cataract surgery through corneal relaxing incision, manually and femtosecond laser assisted. Methods: The study was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement recommendations. We used PubMed, Scopus, and Web of Science (WOS) as databases from January 2010 to March 30, 2021. Patients with keratoconus, corneal ectasia, and a previous history of eye surgery were excluded because our aim was to analyze only healthy eyes. Results: A total of 1025 eyes were evaluated from 946 patients (mean age was 68.90 ± 5.12) in manual incision group articles, while 1905 eyes of 1483 patients (mean age was 65.05 ± 4.57) were evaluated in femtosecond laser arcuate keratotomy (FLAK) articles. The mean uncorrected distance visual acuity (UDVA) was 0.19 ± 0.12 and 0.15 ± 0.05 logMAR for manual incision and FLAK articles, respectively (p = 0.39). The mean correction index (CI) was similar in both groups: 0.77 ± 0.18 in manual incision and 0.79 ± 0.17 in femtosecond laser assisted incision (p = 0.70). Refractive stability was found after 3 months and no serious complications were reported during the follow-up in any group. Conclusion: Both techniques are safe and moderately effective in corneal astigmatism correction in cataract surgery. FLAK represents a more precise and predictable approach. However, since visual and refractive outcomes appear to be similar in both cases, the cost-benefit analysis is controversial

Comparison of wound architecture in implantable collamer lens surgery: Self-sealing single-plane opposite clear corneal incision versus main surgical incision

Purpose: Incision architecture can play an important role in corneal astigmatism management through peripheral corneal relaxing incisions. The aim of this study was to compare the incision architecture of single-plane opposite clear corneal incisions (OCCIs) and main surgical incisions (MSIs) in patients undergoing implantable collamer lens (ICL) surgery. Methods: A retrospective cross-sectional tomographic analysis of MSI and OCCI architectures was performed 6 months after ICL surgery. Image acquisition was performed using spectral-domain anterior segment optical coherence tomography. Results: A total of 31 OCCIs and 24 MSIs were evaluated. The mean incision angle was 42.83 ± 5.69 degrees for MSIs and 48.26 ± 6.07 degrees for OCCIs (p < 0.01), and the mean MSI and OCCI length was 1146.70 ± 150.48 µm and 976.68 ± 140.19 µm, respectively (p < 0.01). The mean increase in epithelium depth in the wound was 37.63 ± 11.91 µm in the MSI group and 47.64 ± 15.45 µm in the OCCI group (p = 0.02). Endothelial misalignment was observed in both types of incisions. However, the misalignment with MSI was greater than with OCCI, 106.67 ± 31.84 µm versus 83.75 ± 23.39 µm (p = 0.01), respectively. Conclusion: Both types of incisions, OCCI and MSI, were shown to be safe with complete wound sealing and healing 6 months postoperatively. The MSIs performed in the temporal position were more angled and longer, with greater endothelial retraction and minor epithelial thickening in the wound area compared with astigmatic incisions without manipulation

Evaluación de factores asociados al estado funcional en ancianos de 60 años o más en Bogotá, Colombia

Introduction: Functional impairment produces a wide range of negative effects such as difficulty in mobility, social isolation, decreased quality of life, disability and institutionalization. Thus, functional status measurement is a marker of social wellbeing.Objective: To determine and characterize the socio-demographic factors and health conditions related to functional impairment in older adults in Bogotá, Colombia.Materials and methods: Data was collected from the SABE Bogotá Study. Functional status was assessed using the Barthel and Lawton scales. Independent variables included socio-demographic factors, comorbidities, anthropometric measurements and physical activity (by tertiles). Bivariate analyzes were performed, and multivariate results were obtained using linear regression models.Results: There were 2,000 participants aged 60 years and older, with a mean age of 71.2 ± 8 years. Younger age (standardized beta = -0.15, p&lt;0.01), fewer medications (beta= -0.13, p&lt;0.01), higher MMSE score (beta = 0.3, p&lt; 0.01), higher level of physical activity (middle beta tertile = 0.18, p&lt;0.01, and higher beta tertile= 0.18, p&lt;0.01vs lower tertile) and better performance in the handgrip test (beta= 0.10, p&lt;0.01) were associated with better function in daily life instrumental activities. Similarly, higher MMSE score (beta= 0.3, p&lt;0.01) and better performance in the handgrip test (beta= 0.07, p= 0.02) were associated with better function in daily life basic activities.Conclusions: We found several factors related with functional impairment, which are likely to be modified to reduce dependence in this population.Introducción. La declinación funcional produce un amplio espectro de efectos negativos, como dificultad para la movilidad, aislamiento social, deterioro de la calidad de vida, discapacidad e internación en instituciones especializadas, lo cual convierte la medición de la funcionalidad en un marcador importante del bienestar.Objetivo. Caracterizar y determinar los factores asociados con el deterioro funcional del anciano en Bogotá.Materiales y métodos. Se utilizaron los datos del Estudio SABE-Bogotá. El estado funcional de los participantes se evaluó mediante las escalas de Barthel y Lawton. Se usaron como variables independientes los factores sociodemográficos, las enfermedades concomitantes, las medidas antropométricas y los terciles de actividad física, y se obtuvieron análisis bivariados y multivariados con regresiones lineales.Resultados. Se entrevistaron 2.000 personas de 60 o más años, con una edad promedio de 71,2 ± 8 años. Una mayor funcionalidad en actividades instrumentales se asoció con una menor edad (valor beta estandarizado: -0,15; p&lt;0,01), un menor número de medicamentos (valor beta: -0,13; p&lt;0,01), un mayor puntaje en el Mini-mental State Examination, MMSE) (valor beta: 0,3; p&lt;0,01), un mayor nivel de actividad física (tercil medio: 0,18; p&lt;0,01 y tercil alto de beta: 0,18; p&lt;0,01 Vs. tercil bajo) yuna mayor fuerza de prensión (valor beta: 0,10; p&lt;0,01),en tanto que una mayor funcionalidad en las actividades básicas se asoció con un mayor puntaje en el MMSE (valor beta: 0,3; p&lt;0,01) y una mayor fuerza de prensión (valor beta: 0,07; p=0,020).Conclusiones. En este estudio se determinaron múltiples factores relacionados con la alteración funcional que pueden modificarse para disminuir la dependencia en este grupo poblacional

Dataset of the sperm proteome of stallions with different motility

This paper provides a detailed set of data on how the stal-lion sperm proteome differs among stallions with different sperm motilities, although within normal ranges. Findings distinguish proteins that may help to identify stallions of su-perior sperm motility. Sperm proteins were analyzed using a UHPLC/MS/MS system comprising of an Agilent 1290 in-finity series UHPLC coupled to an Agilent 6550 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). These data can be used to disclose potential targets to iden-tify good sperm samples and to study specific pathways in-volved in the regulation of sperm motility. This data article is linked to the paper "Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility Journal of Proteomics 247:104335 doi: 10.1016/j.jprot.2021.104335".(c) 2022 The Author(s). Published by Elsevier Inc.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/

Anti-Inflammatory (M2) Response Is Induced by a sp(2)-Iminosugar Glycolipid Sulfoxide in Diabetic Retinopathy

Diabetic retinopathy (DR) is one of the most common complications of Diabetes Mellitus (DM) and is directly associated with inflammatory processes. Currently, neuro-inflammation is considered an early event in DR and proceeds via microglia polarization. A hallmark of DR is the presence of retinal reactive gliosis. Here we report the beneficial effect of (S (S),1R)-1-docecylsulfiny-5N,6O-oxomethylidenenojirimycin ((Ss)-DS-ONJ), a member of the sp(2)-iminosugar glycolipid (sp(2)-IGL) family, by decreasing iNOS and inflammasome activation in Bv.2 microglial cells exposed to pro-inflammatory stimuli. Moreover, pretreatment with (Ss)-DS-ONJ increased Heme-oxygenase (HO)-1 as well as interleukin 10 (IL10) expression in LPS-stimulated microglial cells, thereby promoting M2 (anti-inflammatory) response by the induction of Arginase-1. The results strongly suggest that this is the likely molecular mechanism involved in the anti-inflammatory effects of (S (S))-DS-ONJ in microglia. (S (S))-DS-ONJ further reduced gliosis in retinal explants from type 1 diabetic BB rats, which is consistent with the enhanced M2 response. In conclusion, targeting microglia polarization dynamics in M2 status by compounds with anti-inflammatory activities offers promising therapeutic interventions at early stages of DR

LIFE Adaptamed Layman’s Report. Action E13. LIFE14 CCA/ES/000612

Aguas de Font Vella y Lanjaró