Technical Note: A rotary seed processor for removing pubescence from seed of prairie grasses

Many of the perennial prairie grasses that are used in restoration plantings in the central Great Plains have seed appendages such as awns and pubescence that make seed flow through planters difficult. We have developed a rotary seed processor that efficiently processes small breeder or experimental lots of seed that can then be easily planted with small plot cone planters or conventional planters. The processor consists of a metal cylinder that is lined with corrugated rubber and a rotating center shaft with rubber paddles. Processing can be controlled by varying shaft rotation speed and processing time. A top-opening, full length trap door allows for easy loading and the cylinder can be inverted to dump out processed seed. The processor has been used successfully for several years on big bluestem [Andropogon gerardii Vitman], indiangrass [Sorghastrum nutans (L.) Nash], little bluestem [Schizachyrium scoparium (Michaux) Nash], prairie sandreed [Calamovilfa longifolia (Book.) Scribner], and blue grama [Boureloua gracilis (Willd. ex Kunth) Lagascaex Griffiths] seed. By removing seed appendages and pubescence, seed bulk is reduced and seed density and flow ability are improved. The processing operation is relatively gentle and seed germination per unit weight of seed is improved. Semillas de muchos pastos perennes que son usados en la restauracion de praderas en las Grandes Planicies centrales, poseen accesorios coma aristas y/o pubescencias que difcultan el paso de las semillas a traves de sembradoras convencionales. Para efectuar siembras con btas semillas, las semhradoras tendrian que estar especificamente diieiiadas para plantar material relativamente voluminoso y sin peso, al menos que las semillas scan procesadas para remover tales cuerpos accesorios. Nosotros hemos desarrollado un procesador de semilla que process eticientemente pequeiios lotes de semillas con fries de reproduction de germoplasma o experimentales. De &a manera, las semillas podran ser facilmente sembrados con un sembrador de cone para pequefias parcelas, o con sembradoras convencionales. El procesador consiste de un cilindro o tambor de metal forrado en su interior con hule corrugado. Paletas de hule adjuntas a un eje que atraviesa el cilindro y que son rotadas por polea, golpean las semillas. El proceso puede ser controlado variando el tamaiio de polea y la velocidad de rotacibn, y por el tiempo que la semilla permanece rotando dentro de la unidad. El cilindro cuenta con una puerta en la parte superior que permite el facil acceso de la semilla dentro de la unidad. Cuando la semilla ha sido procesada, el cilindro puede ser invertido para retirar la semilla de la unidad. El procesador ha sido exitosamente usado por varios aiios en big bluestem (Andropogon gerardii Vitman), indiangrass [Sorghastrum nutans (L.) Nash], little bluestem [Schizachyrium scoparium (Michx.) Nash], prairie sandreed [Calamovilfa longifolia (Hook.) Scribn.], and blue grama [Bouteloua gracilis (Wiid. ex Kunth) Lagasca ex Griffiths] seed. La remocion de las aristas y pubescencias disminuyen el volumen de la semi&, y la densidad y flujo dentro de la sembradora son entonces mejorados. El proceso de operation es relativamente suave para las semillas y la genninacion por unidad de peso es mejorada

Fall, Recovery and Characterization of the Novato L6 Chondrite Breccia

The Novato L6 chondrite fragmental breccia fell in California on 17 October 2012, and was recovered after the Cameras for Allsky Meteor Surveillance (CAMS) project determined the meteor's trajectory between 95 and 45 km altitude. The final fragmentation at 33 1 km altitude was exceptionally well documented by digital photographs. The first sample was recovered before rain hit the area. First results from a consortium study of the meteorite's characterization, cosmogenic and radiogenic nuclides, origin and conditions of the fall are presented. Some meteorites did not retain fusion crust and show evidence of spallation. Before entry, the meteoroid was 35+/-5 cm in diameter (mass 80+/-35 kg) with a cosmic ray exposure age of 9+/-1 Ma, if it had a one-stage exposure history. However, based on the cosmogenic nuclide inventory, a two-stage exposure history is more likely, with lower shielding in the last few Ma. Thermoluminescence data suggest a collision event within the last approx. 0.1 Ma. Novato likely belonged to the class of shocked L chondrites that have a common shock age of 470 Ma, based on the U,Th-He age of 460+/-220 Ma. The measured orbits of Novato, Jesenice and Innisfree are consistent with a proposed origin of these shocked L chondrites in the Gefion asteroid family, but leave open the possibility that they came to us directly from the 5:2 mean motion resonance with Jupiter. Novato experienced a stronger compaction than did other L6 chondrites of shock-stage S4. Despite this, a freshly broken surface shows a wide range of organic compounds

LSST: from Science Drivers to Reference Design and Anticipated Data Products

(Abridged) We describe here the most ambitious survey currently planned in the optical, the Large Synoptic Survey Telescope (LSST). A vast array of science will be enabled by a single wide-deep-fast sky survey, and LSST will have unique survey capability in the faint time domain. The LSST design is driven by four main science themes: probing dark energy and dark matter, taking an inventory of the Solar System, exploring the transient optical sky, and mapping the Milky Way. LSST will be a wide-field ground-based system sited at Cerro Pach\'{o}n in northern Chile. The telescope will have an 8.4 m (6.5 m effective) primary mirror, a 9.6 deg$^2$ field of view, and a 3.2 Gigapixel camera. The standard observing sequence will consist of pairs of 15-second exposures in a given field, with two such visits in each pointing in a given night. With these repeats, the LSST system is capable of imaging about 10,000 square degrees of sky in a single filter in three nights. The typical 5$\sigma$ point-source depth in a single visit in $r$ will be $\sim 24.5$ (AB). The project is in the construction phase and will begin regular survey operations by 2022. The survey area will be contained within 30,000 deg$^2$ with $\delta<+34.5^\circ$, and will be imaged multiple times in six bands, $ugrizy$, covering the wavelength range 320--1050 nm. About 90\% of the observing time will be devoted to a deep-wide-fast survey mode which will uniformly observe a 18,000 deg$^2$ region about 800 times (summed over all six bands) during the anticipated 10 years of operations, and yield a coadded map to $r\sim27.5$. The remaining 10\% of the observing time will be allocated to projects such as a Very Deep and Fast time domain survey. The goal is to make LSST data products, including a relational database of about 32 trillion observations of 40 billion objects, available to the public and scientists around the world.Comment: 57 pages, 32 color figures, version with high-resolution figures available from https://www.lsst.org/overvie

Inherited variation in immune genes and pathways and glioblastoma risk

To determine whether inherited variations in immune function single-nucleotide polymorphisms (SNPs), genes or pathways affect glioblastoma risk, we analyzed data from recent genome-wide association studies in conjunction with predefined immune function genes and pathways. Gene and pathway analyses were conducted on two independent data sets using 6629 SNPs in 911 genes on 17 immune pathways from 525 glioblastoma cases and 602 controls from the University of California, San Francisco (UCSF) and a subset of 6029 SNPs in 893 genes from 531 cases and 1782 controls from MD Anderson (MDA). To further assess consistency of SNP-level associations, we also compared data from the UK (266 cases and 2482 controls) and the Mayo Clinic (114 cases and 111 controls). Although three correlated epidermal growth factor receptor (EGFR) SNPs were consistently associated with glioblastoma in all four data sets (Mantel–Haenzel P values = 1 × 10−5 to 4 × 10−3), independent replication is required as genome-wide significance was not attained. In gene-level analyses, eight immune function genes were significantly (minP < 0.05) associated with glioblastoma; the IL-2RA (CD25) cytokine gene had the smallest minP values in both UCSF (minP = 0.01) and MDA (minP = 0.001) data sets. The IL-2RA receptor is found on the surface of regulatory T cells potentially contributing to immunosuppression characteristic of the glioblastoma microenvironment. In pathway correlation analyses, cytokine signaling and adhesion–extravasation–migration pathways showed similar associations with glioblastoma risk in both MDA and UCSF data sets. Our findings represent the first systematic description of immune genes and pathways that characterize glioblastoma risk

Transforming Growth Factor: β Signaling Is Essential for Limb Regeneration in Axolotls

Axolotls (urodele amphibians) have the unique ability, among vertebrates, to perfectly regenerate many parts of their body including limbs, tail, jaw and spinal cord following injury or amputation. The axolotl limb is the most widely used structure as an experimental model to study tissue regeneration. The process is well characterized, requiring multiple cellular and molecular mechanisms. The preparation phase represents the first part of the regeneration process which includes wound healing, cellular migration, dedifferentiation and proliferation. The redevelopment phase represents the second part when dedifferentiated cells stop proliferating and redifferentiate to give rise to all missing structures. In the axolotl, when a limb is amputated, the missing or wounded part is regenerated perfectly without scar formation between the stump and the regenerated structure. Multiple authors have recently highlighted the similarities between the early phases of mammalian wound healing and urodele limb regeneration. In mammals, one very important family of growth factors implicated in the control of almost all aspects of wound healing is the transforming growth factor-beta family (TGF-β). In the present study, the full length sequence of the axolotl TGF-β1 cDNA was isolated. The spatio-temporal expression pattern of TGF-β1 in regenerating limbs shows that this gene is up-regulated during the preparation phase of regeneration. Our results also demonstrate the presence of multiple components of the TGF-β signaling machinery in axolotl cells. By using a specific pharmacological inhibitor of TGF-β type I receptor, SB-431542, we show that TGF-β signaling is required for axolotl limb regeneration. Treatment of regenerating limbs with SB-431542 reveals that cellular proliferation during limb regeneration as well as the expression of genes directly dependent on TGF-β signaling are down-regulated. These data directly implicate TGF-β signaling in the initiation and control of the regeneration process in axolotls