A systematic review of Twitter’s hashtags in public health: an example of a globally adopted standard

The objective is to find evidence and make recommendations on the use of Twitter in public health,particularly through the study of hashtags(#). This systematic review shows the use of Twitter in different areas of public health: epidemiological surveillance, health promotion, health protection and disease prevention. Articles on this subject published in indexed journals with impact factor show the importance of conversations to engage the attention of Twitter users by using citations (@ user) and retweets(RT); however, not much importance seems to be given to the use of hashtags(#), which are often assimilated to the concept of keywords. Although tracking recurring hashtagsshould be less expensive than computing Twitter content, the potential of hashtaggeddata has not been properly exploited or recognized over the past years, probably due to the lack of efficient tools

Analysis of genetic variability of commercial melon cultivars using SSR molecular markers.

Made available in DSpace on 2018-01-12T23:14:13Z (GMT). No. of bitstreams: 1 GeneticsandMolecularResearchv.16n.32017CARVALHOetal.pdf: 264981 bytes, checksum: 7705c965bc736f07bd7bd0d922d75cee (MD5) Previous issue date: 2017-11-14bitstream/item/166721/1/Genetics-and-Molecular-Research-v.-16-n.-3-2017-CARVALHO-et-al.pd

Correction to: Dimensions of leisure-time physical activity and risk of depression in the “Seguimiento Universidad de Navarra” (SUN) prospective cohort

After publication of our article [1] we have been notified that Table 2 was incorrectly formatte

Análise da variabilidade genética de linhagens de melão do tipo pele de sapo utilizando marcadores SSR e ISSR.

Nas últimas duas décadas, o agronegócio do melão no Brasil teve um crescimento de mais de 800%. Dentro deste mercado, a variedade tipo pele de sapo representa, atualmente, de 15% a 18% da área plantada. Estudos de variabilidade genética que utilizam marcadores moleculares auxiliam programas de melhoramento, possibilitando a seleção de linhagens parentais para a obtenção de populações com efeitos heteróticos significativos e híbridos superiores. Com esse objetivo, 58 linhagens de melão do tipo pele de sapo foram analisadas utilizando-se marcadores SSR e ISSR. A separação dos fragmentos SSR foi realizada por meio de eletroforese vertical em gel de poliacrilamida 5% e coloração com nitrato de prata e a dos fragmentos ISSRs, em eletroforese horizontal em gel de agarose 1,5% corado com brometo de etídeo. Os marcadores SSR amplificaram um total de 45 alelos para os 58 genótipos, enquanto que os marcadores ISSR amplificaram 74 bandas polimórficas. Os dendrogramas gerados por ambos os marcadores (SSR e ISSR) apresentaram resultados satisfatórios, permitindo-se sugerir maior heterose pelo cruzamento entre linhagens dos principais grupos divergentes, e a utilização das linhagens 43, 1 e 25, provavelmente, aumentará a variabilidade alélica no programa de melhoramento de eloeiro da Embrapa.bitstream/item/186054/1/Boletim-melao-peledesapo3.pd

Quaternary structure of a G-protein coupled receptor heterotetramer in complex with Gi and Gs

Background: G-protein-coupled receptors (GPCRs), in the form of monomers or homodimers that bind heterotrimeric G proteins, are fundamental in the transfer of extracellular stimuli to intracellular signaling pathways. Different GPCRs may also interact to form heteromers that are novel signaling units. Despite the exponential growth in the number of solved GPCR crystal structures, the structural properties of heteromers remain unknown. Results: We used single-particle tracking experiments in cells expressing functional adenosine A1-A2A receptors fused to fluorescent proteins to show the loss of Brownian movement of the A1 receptor in the presence of the A2A receptor, and a preponderance of cell surface 2:2 receptor heteromers (dimer of dimers). Using computer modeling, aided by bioluminescence resonance energy transfer assays to monitor receptor homomerization and heteromerization and G-protein coupling, we predict the interacting interfaces and propose a quaternary structure of the GPCR tetramer in complex with two G proteins. Conclusions: The combination of results points to a molecular architecture formed by a rhombus-shaped heterotetramer, which is bound to two different interacting heterotrimeric G proteins (Gi and Gs). These novel results constitute an important advance in understanding the molecular intricacies involved in GPCR function

Cognitive impairment induced by delta9-tetrahydrocannabinol occurs through heteromers between cannabinoid CB1 and serotonin 5-HT2A receptors

Delta-9-tetrahydrocannabinol (THC), the main psychoactive compound of marijuana, induces numerous undesirable effects, including memory impairments, anxiety, and dependence. Conversely, THC also has potentially therapeutic effects, including analgesia, muscle relaxation, and neuroprotection. However, the mechanisms that dissociate these responses are still not known. Using mice lacking the serotonin receptor 5-HT2A, we revealed that the analgesic and amnesic effects of THC are independent of each other: while amnesia induced by THC disappears in the mutant mice, THC can still promote analgesia in these animals. In subsequent molecular studies, we showed that in specific brain regions involved in memory formation, the receptors for THC and the 5-HT2A receptors work together by physically interacting with each other. Experimentally interfering with this interaction prevented the memory deficits induced by THC, but not its analgesic properties. Our results highlight a novel mechanism by which the beneficial analgesic properties of THC can be dissociated from its cognitive side effects

Effects of a Mediterranean Eating Plan on the Need for Glucose-Lowering Medications in Participants With Type 2 Diabetes: A Subgroup Analysis of the PREDIMED Trial

[Objective]: To examine the effects of two Mediterranean eating plans (Med-EatPlans) versus a low-fat eating plan on the need for glucose-lowering medications. [Research design and methods]: From the Prevención con Dieta Mediterránea (PREDIMED) trial, we selected 3,230 participants with type 2 diabetes at baseline. These participants were randomly assigned to one of three eating plans: Med-EatPlan supplemented with extra-virgin olive oil (EVOO), Med-EatPlan supplemented with mixed nuts, or a low-fat eating plan (control). In a subgroup (15%), the allocation was done in small clusters instead of using individual randomization, and the clustering effect was taken into account in the statistical analysis. In multivariable time-to-event survival models, we assessed two outcomes: 1) introduction of the first glucose-lowering medication (oral or injectable) among participants on lifestyle management at enrollment and 2) insulin initiation. [Results]: After a median follow-up of 3.2 years, in multivariable analyses adjusting for baseline characteristics and propensity scores, the hazard ratios (HRs) of starting a first glucose-lowering medication were 0.78 (95% CI 0.62–0.98) for Med-EatPlan + EVOO and 0.89 (0.71–1.12) for Med-EatPlan + nuts, compared with the control eating plan. After a median follow-up of 5.1 years, the adjusted HRs of starting insulin treatment were 0.87 (0.68–1.11) for Med-EatPlan + EVOO and 0.89 (0.69–1.14) for Med-EatPlan + nuts compared with the control eating plan. [Conclusions]: Among participants with type 2 diabetes, a Med-EatPlan + EVOO may delay the introduction of new-onset glucose-lowering medications. The Med-EatPlan did not result in a significantly lower need for insulin