Portfolio Vol. I N 3

Sweitzer, Harry J. Portfolio Goes to Press . Prose. 1. Browne, Phil. William Howard Doane Library . Picture. 2. Overhuls, James. Out of Himself . Prose. 3. MacNeill, Annie Marie. To President and Mrs. Shaw . Poem. 6. Baker, George. Saint in a Silo . Prose 7. Beckham, Adela. In Moods . Poem. 8. Vincent, Charles. Incident of August 7, 1930. Prose. 9. Flory, Doris. Opinions . Poem. 10. Flory, Doris. Thoughts in Spring . Poem. 10. Flory, Doris. Breakfast Scene . Poem. 10. Shaw, Robert B. A Date for the Dances . Prose. 11. Cronberger, Barbara. And the Years Go On . Prose. 13. Hanna, Stanley. Reola, Reola . Poem. 14. Hanna, Stanley. The Dance of the Kobolds . Poem. 14. Nadel, Norman. I died Last Night . Prose. 15. Bethune, Don S. Adolescence . Poem 16. Vodev, Eugene. The Black Day of Bulgaria . Prose. 17. Dick, Pewilla. To a White Violet . Poem. 18. Dick, Pewilla. As With Your Shadow . Poem. 18. Dwelly, Thorndike. Of Mice and Men . Prose. 19. Clements, Helen. Our Town . Prose. 19. Schlle, Alice. Marion is an Old Costume . Picture. 20. Chadeayne, Robert. Factory . Picture. 20. Nadel, Norman. Dmitri Shostakovitch . Prose. 21. Stewart, John. Duke Ellington\u27s Records . Prose. 21. Beck, Virginia. The Dance as an Art . Prose. 22. Dick, Pewilla. Death . Poem. 23. Flory, Doris. On Reforms . Poem. 24. Beckham, Adela. The Lie . Poem. 24. Bethune, Don. Futility . Poem. 24

Functional comparison of blood-stage Plasmodium falciparum malaria vaccine candidate antigens

The malaria genome encodes over 5,000 proteins and many of these have also been proposed to be potential vaccine candidates, although few of these have been tested clinically. RH5 is one of the leading blood-stage Plasmodium falciparum malaria vaccine antigens and Phase I/II clinical trials of vaccines containing this antigen are currently underway. Its likely mechanism of action is to elicit antibodies that can neutralize merozoites by blocking their invasion of red blood cells (RBC). However, many other antigens could also elicit neutralizing antibodies against the merozoite, and most of these have never been compared directly to RH5. The objective of this study was to compare a range of blood-stage antigens to RH5, to identify any antigens that outperform or synergize with anti-RH5 antibodies. We selected 55 gene products, covering 15 candidate antigens that have been described in the literature and 40 genes selected on the basis of bioinformatics functional prediction. We were able to make 20 protein-in-adjuvant vaccines from the original selection. Of these, S-antigen and CyRPA robustly elicited antibodies with neutralizing properties. Anti-CyRPA IgG generally showed additive GIA with anti-RH5 IgG, although high levels of anti-CyRPA-specific rabbit polyclonal IgG were required to achieve 50% GIA. Our data suggest that further vaccine antigen screening efforts are required to identify a second merozoite target with similar antibody-susceptibility to RH5

Portfolio Vol. II N 1

Browne, Phil. The Approach to Fraternity Row . Picture. 2. Simmons, Fate. The Sand House . Prose. 3. The College Catbird, Groucho. Ode to my Fellow Students . Poem. 6. Varney, Chester. The Tramp . Prose. 7. Browne, Phil. Shell Shock . Prose. 9. West, Bill C. Mr. Freud... . Poem. 10. West, Bill C. Bacchanal . Poem. 10. De Chavannes, Pierre Puvis de. Summer . Poem. 10. Pierce, Ames. A Student Looks at Europe . Prose. 11. Timrud, David. Though you Knew it Not . Poem. 13. Timrud, David. Le Joi De Vivre . Poem. 13. Timrud, David. The Ghostly Loom . Poem. 13. Dohanos, Stephen. West Quoddy Light, Maine . Picture. 13. Millet, Jean Francois. Peasants Going to Work . Picture. 14. Kent, Rockwell. Maine Coast . Picture. 14. Beier, Dean. Review of New Recordings . Prose. 15. Beier, Dean. Advice on Band Booking . Prose. 15. Millay, Edna St. Vincent. From \u27Conversation at Midnight\u27 . Prose. 16. Black, James. Playing Around . Prose. 17. Saunders, Paul. Review of New Books .Prose. 17. Salietti, Alberto. A country Woman . Picture. 18. Eschman, Barbara. Color Scheme . Poem. 18. Whitehead, Richard. A Tribute . Picture. 19. Beckham, Adela. Gethsemane . Poem. 20. Beckham, Adela. Blues Singer . Poem. 20. Flory, Doris. Revelation . Poem. 20. Flory, Doris. Fervor . Poem. 20. Hanna, Stanley. Men of Fortune . Poem. 20. Sweitzer, Harry J. Denison and Education . Prose. 21. Hopkins, Kate. Twillight . Prose. 23. Hopkins, Kate. Afterward . Prose. 23

Complement as an Endogenous Adjuvant for Dendritic Cell-Mediated Induction of Retrovirus-Specific CTLs

Previous studies have demonstrated the involvement of complement (C) in induction of efficient CTL responses against different viral infections, but the exact role of complement in this process has not been determined. We now show that C opsonization of retroviral particles enhances the ability of dendritic cells (DCs) to induce CTL responses both in vitro and in vivo. DCs exposed to C-opsonized HIV in vitro were able to stimulate CTLs to elicit antiviral activity significantly better than non-opsonized HIV. Furthermore, experiments using the Friend virus (FV) mouse model illustrated that the enhancing role of complement on DC-mediated CTL induction also occurred in vivo. Our results indicate that complement serves as natural adjuvant for DC-induced expansion and differentiation of specific CTLs against retroviruses

Trouble with Bleeding: Risk Factors for Acute Hepatitis C among HIV-Positive Gay Men from Germany—A Case-Control Study

OBJECTIVES: To identify risk factors for hepatitis C among HIV-positive men who have sex with men (MSM), focusing on potential sexual, nosocomial, and other non-sexual determinants. BACKGROUND: Outbreaks of hepatitis C virus (HCV) infections among HIV-positive MSM have been reported by clinicians in post-industrialized countries since 2000. The sexual acquisition of HCV by gay men who are HIV positive is not, however, fully understood. METHODS: Between 2006 and 2008, a case-control study was embedded into a behavioural survey of MSM in Germany. Cases were HIV-positive and acutely HCV-co-infected, with no history of injection drug use. HIV-positive MSM without known HCV infection, matched for age group, served as controls. The HCV-serostatus of controls was assessed by serological testing of dried blood specimens. Univariable and multivariable regression analyses were used to identify factors independently associated with HCV-co-infection. RESULTS: 34 cases and 67 controls were included. Sex-associated rectal bleeding, receptive fisting and snorting cocaine/amphetamines, combined with group sex, were independently associated with case status. Among cases, surgical interventions overlapped with sex-associated rectal bleeding. CONCLUSIONS: Sexual practices leading to rectal bleeding, and snorting drugs in settings of increased HCV-prevalence are risk factors for acute hepatitis C. We suggest that sharing snorting equipment as well as sharing sexual partners might be modes of sexual transmission. Condoms and gloves may not provide adequate protection if they are contaminated with blood. Public health interventions for HIV-positive gay men should address the role of blood in sexual risk behaviour. Further research is needed into the interplay of proctosurgery and sex-associated rectal bleeding

Mapping 123 million neonatal, infant and child deaths between 2000 and 2017

Since 2000, many countries have achieved considerable success in improving child survival, but localized progress remains unclear. To inform efforts towards United Nations Sustainable Development Goal 3.2—to end preventable child deaths by 2030—we need consistently estimated data at the subnational level regarding child mortality rates and trends. Here we quantified, for the period 2000–2017, the subnational variation in mortality rates and number of deaths of neonates, infants and children under 5 years of age within 99 low- and middle-income countries using a geostatistical survival model. We estimated that 32% of children under 5 in these countries lived in districts that had attained rates of 25 or fewer child deaths per 1,000 live births by 2017, and that 58% of child deaths between 2000 and 2017 in these countries could have been averted in the absence of geographical inequality. This study enables the identification of high-mortality clusters, patterns of progress and geographical inequalities to inform appropriate investments and implementations that will help to improve the health of all populations

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data