VALIDAÇÃO DO TEMPO DE VIDA ÚTIL APRESENTADO POR SENSOR FOTOCRÔMICO DE TEMPO E TEMPERATURA

Estudou-se o comportamento de indicador fotocrômico de tempo e temperatura (ITT) com o objetivo de analisar a influencia de diferentes tempos de ativação (exposição à luz ultravioleta) sobre o processo de descoloração da etiqueta, caracterizando sua vida útil em condições específicas de temperatura. Os valores de desvanecimento da cor do ITT, quantificados mediante o chroma b* (CIELab) em função do tempo, apontaram que a descoloração do rótulo é diretamente proporcional à quantidade de luz usada no processo de ativação e inversamente proporcional à temperatura de armazenamento da etiqueta. Devido à possibilidade de definir a vida útil do ITT de forma reprodutível concluiu-se que esse dispositivo constitui ferramenta confiável para monitorar a cadeia de frio de muitos produtos alimentares

COGNITION AND FUNCTIONALITY IN BREAST CANCER SURVIVORS UNDERGOING HORMONE THERAPY

O objetivo foi descrever a função cognitiva e a capacidade funcional de sobreviventes de câncer de mama que praticam exercício físico. Oito mulheres diagnosticadas com câncer de mama em estágios I-III participaram deste estudo. Testes cognitivos foram aplicados para avaliar a função cognitiva geral, velocidade de processamento, função executiva e atenção. A capacidade funcional foi determinada por testes de resistência aeróbia, agilidade dinâmica e equilíbrio e testes de força de membros superiores e inferiores. As mulheres do presente estudo tinham 64,5 ± 4,8 anos, índice de massa corporal de 32,6 ± 5,4 kg.m-2 e escolaridade de 10,0 ± 3,5 anos. Os resultados demonstraram desempenho no questionário MSSE e teste de fluência verbal preservados em mulheres idosas diagnosticadas com câncer de mama há quatro anos, com obesidade e que estavam se exercitando por aproximadamente 2,5 anos. Por outro lado, elas apresentaram desempenho inferior no teste cognitivo Trail Making Test e nos testes funcionais do que os valores normativos da literatura, indicando um prejuízo na atenção, velocidade de processamento, flexibilidade cognitiva e baixa capacidade funcional.El objetivo fue describir la función cognitiva y la capacidad funcional de las supervivientes de cáncer de mama que practican ejercicio físico. Ocho mujeres diagnosticadas con cáncer de mama en estadio I-III participaron en este estudio. Se aplicaron pruebas cognitivas para evaluar la función cognitiva general, la velocidad de procesamiento, la función ejecutiva y la atención. La capacidad funcional se determinó mediante pruebas de resistencia aeróbica, agilidad dinámica y equilibrio y pruebas de fuerza superior e inferior. Las mujeres del presente estudio tenían 64,5 ± 4,8 años, el índice de masa corporal 32,6 ± 5,4 kg.m-2 y la escolaridad de 10,0 ± 3,5 años. Los resultados demostraron un desempeño preservado en el cuestionario MSSE y la prueba de fluidez verbal en mujeres mayores diagnosticadas con cáncer de mama hace cuatro años, con obesidad y que hacían ejercicio durante aproximadamente 2,5 años. Por otro lado, ellas presentaron un desempeño más bajo en el Trail Making Test cognitivo y en las pruebas funcionales que los valores normativos de la literatura, indicando un deterioro en la atención, velocidad de procesamiento, flexibilidad cognitiva y baja capacidad funcional.The study aimed to describe the cognitive function and the functional capacity of breast cancer survivors who practice physical exercise. Eight women diagnosed with stages I-III breast cancer participated in this study. Cognitive tests were applied to assess overall cognitive function, processing speed, executive function, and attention. The women in the present study were 64.5 ± 4.8 years old, had a body mass index of 32.6 ± 5.4 kg.m-2, and had 10.0 ± 3.5 years of schooling. The results showed a preserved performance in the MSSE questionnaire and verbal fluency test in older women diagnosed with breast cancer four years ago, with obesity, who have been exercising for approximately 2.5 years. On the other hand, they presented a lower performance in the cognitive Trail Making Test and the functional tests than the normative values in the literature, indicating an impaired attention, processing speed, cognitive flexibility, and low functional capacity

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Correction to: Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

The original version of this article unfortunately contained a mistake

Microbiological evaluation of chicken feet intended for human consumption

Chicken feet are products with great commercial importance for the eastern markets. Although Brazil is a large exporter of these products to those markets, little information is available on the sanitary quality of these products. The objective of this study was to evaluate the microbiological quality of frozen chicken feet for human consumption. This study was developed in a slaughterhouse under Federal Inspection, located in the state of Rio Grande do Sul. A total of 98 samples of frozen chicken feet were analyzed, between January and December 2011, for the detection of Salmonella spp., total count of mesophilic bacteria, total coliforms, fecal coliforms, Escherichia coli, Staphylococcus coagulase positive and Clostridium perfringens. About 99% of the results were within the microbiological standards established by the Chinese (world´s largest importer) and Brazilian legislation for raw chicken meat. Thus, we conclude that the samples of frozen chicken feet showed satisfactory microbiological quality and no risk to consumer health

Survey of extended-spectrum beta-lactamases in Escherichia coli isolates from a Portuguese hospital and characterisation of a novel class 1 integron (In60A) carrying the bla(CTX-M-9) gene

Between November 2001 and November 2004, 231 Escherichia coli isolates resistant to P-lactam antibiotics were identified. In 14 isolates, bla(TEM-24) (2 isolates), bla(TEM-52) (5 isolates) and bla(TEM-26) (7 isolates) were identified. In 145 E. coli isolates with the same M13 fingerprinting profile and the same resistance phenotype, the bla(CTX-M-15) gene was found in association with an insertion sequence ISEcp1. The bla(CTX-M-2) gene was identified in one E. coli isolate (290HSM), and in other E. coli isolate (246HSM) the bla(CTX-M-9) gene was contained in a new complex sull-type class 1 integron (named In60A). This is the first report of three cefotaximases (CTX-M-15, CTX-M-2 and CTX-M-9) in E. coli isolates from a Portuguese hospital. (c) 2006 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved

Role of Key Residues at the Flavin Mononucleotide (FMN):Adenylyltransferase Catalytic Site of the Bifunctional Riboflavin Kinase/Flavin Adenine Dinucleotide (FAD)  Synthetase from Corynebacterium ammoniagenes

In mammals and in yeast the conversion of Riboflavin (RF) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) is catalysed by the sequential action of two enzymes: an ATP:riboflavin kinase (RFK) and an ATP:FMN adenylyltransferase (FMNAT). However, most prokaryotes depend on a single bifunctional enzyme, FAD synthetase (FADS), which folds into two modules: the C-terminal associated with RFK activity and the N-terminal associated with FMNAT activity. Sequence and structural analysis suggest that the 28-HxGH-31, 123-Gx(D/N)-125 and 161-xxSSTxxR-168 motifs from FADS must be involved in ATP stabilisation for the adenylylation of FMN, as well as in FAD stabilisation for FAD phyrophosphorolysis. Mutants were produced at these motifs in the Corynebacterium ammoniagenes FADS (CaFADS). Their effects on the kinetic parameters of CaFADS activities (RFK, FMNAT and FAD pyrophosphorilase), and on substrates and product binding properties indicate that H28, H31, N125 and S164 contribute to the geometry of the catalytically competent complexes at the FMNAT-module of CaFADS