Human Occupancy as a Source of Indoor Airborne Bacteria

Exposure to specific airborne bacteria indoors is linked to infectious and noninfectious adverse health outcomes. However, the sources and origins of bacteria suspended in indoor air are not well understood. This study presents evidence for elevated concentrations of indoor airborne bacteria due to human occupancy, and investigates the sources of these bacteria. Samples were collected in a university classroom while occupied and when vacant. The total particle mass concentration, bacterial genome concentration, and bacterial phylogenetic populations were characterized in indoor, outdoor, and ventilation duct supply air, as well as in the dust of ventilation system filters and in floor dust. Occupancy increased the total aerosol mass and bacterial genome concentration in indoor air PM10 and PM2.5 size fractions, with an increase of nearly two orders of magnitude in airborne bacterial genome concentration in PM10. On a per mass basis, floor dust was enriched in bacterial genomes compared to airborne particles. Quantitative comparisons between bacterial populations in indoor air and potential sources suggest that resuspended floor dust is an important contributor to bacterial aerosol populations during occupancy. Experiments that controlled for resuspension from the floor implies that direct human shedding may also significantly impact the concentration of indoor airborne particles. The high content of bacteria specific to the skin, nostrils, and hair of humans found in indoor air and in floor dust indicates that floors are an important reservoir of human-associated bacteria, and that the direct particle shedding of desquamated skin cells and their subsequent resuspension strongly influenced the airborne bacteria population structure in this human-occupied environment. Inhalation exposure to microbes shed by other current or previous human occupants may occur in communal indoor environments

Cultural Resource Investigations Along the Rocky Reach Reservoir: The 1990 Test Excavations

106-page report, but lacks Chapter 2; Abstract: Cultural resources investigations were conducted at two sensitive areas and fourteen sites along the Rocky Reach Reservoir for the Chelan County Public Utility District No. 1 of Wenatchee, Washington. Because of anticipated impacts from a proposed pool raise, evaluations of significance of these areas/sites were necessary to prepare Determinations of Eligibility. Various methods used to determine the horizontal and vertical extent of the cultural deposits included identifying and mapping exposed materials, and excavation of test units and linear transects of shovel test holes. "The results of the investigation indicate the area was used sporadically over the last 4000 years. The recovered items suggest large mammals were processed for meat and hides at temporary campsites. Recommendations for future work include monitoring sites 45DO409, 45DO504, 45DO505, and 45DO507 at two-year intervals, monitor site 45CH254 at one-year intervals and implement limited data recovery, and conduct data recovery excavations at sites 45CH404, 45DO508, and 45D0511.

Characterizing airborne fungal and bacterial concentrations and emission rates in six occupied children's classrooms

Baseline information on size-resolved bacterial, fungal, and particulate matter (PM) indoor air concentrations and emission rates is presented for six school classrooms sampled in four countries. Human occupancy resulted in significantly elevated airborne bacterial (81 times on average), fungal (15 times), and PM mass (nine times) concentrations as compared to vacant conditions. Occupied indoor/outdoor (I/O) ratios consistently exceeded vacant I/O ratios. Regarding size distributions, average room-occupied bacterial, fungal, and PM geometric mean particle sizes were similar to one another while geometric means estimated for bacteria, fungi, and PM mass during vacant sampling were consistently lower than when occupied. Occupancy also resulted in elevated indoor bacterial-to-PM mass-based and number-based ratios above corresponding outdoor levels. Mean emission rates due to human occupancy were 14 million cells/person/h for bacteria, 14 million spore equivalents/person/h for fungi, and 22 mg/person/h for PM mass. Across all locations, indoor emissions contributed 83 ± 27% (bacteria), 66 ± 19% (fungi), and 83 ± 24% (PM mass) of the average indoor air concentrations during occupied times. © 2015 John Wile