Market Sampling of Landings of Commercial Fish Species in the Netherlands in 2002

This report contains information on the biological sampling for the market sampling program: which species are sampled, how they were caught, when and where the samples are taken (date and position), how many fish have been measured, how many fish have been aged, etc. The report gives an overview of all the biological sampling activities in 2002 by RIVO on the landings of the commercial important species of herring, mackerel, horse mackerel, blue whiting, greater argentine, sole, plaice, turbot, brill, dab, lemon sole, cod, whiting, Norway lobster and four different species of rays from all ICES areas. This biological sampling took place on landings by both the Dutch fleet as well as foreign fleets landing in the Netherlands. In addition this report contains information on the biological samples collected during research vessel surveys and discard trips both on commercial and non-commercial species

The Evolution of NGC 7027 at Radio Frequencies: A New Determination of the Distance and Core Mass

We present the results of a 25-year program to monitor the radio flux evolution of the planetary nebula NGC7027. We find significant evolution of the spectral flux densities. The flux density at 1465 MHz, where the nebula is optically thick, is increasing at a rate of 0.251+-0.015 % per year, caused by the expansion of the ionized nebula. At frequencies where the emission is optically thin, the spectral flux density is changing at a rate of -0.145+-0.005 % per year, caused by a decrease in the number of ionizing photons coming from the central star. A distance of 980+-100 pc is derived. By fitting interpolated models of post-AGB evolution to the observed changes, we find that over the 25-yr monitoring period, the stellar temperature has increased by 3900+-900 K and the stellar bolometric luminosity has decreased by 1.75+-0.38 %. We derive a distance-independent stellar mass of 0.655+-0.01 solar masses adopting the Bloecker stellar evolution models, or about 0.04 solar masses higher when using models of Vassiliadis & Wood which may provide a better fit. A Cloudy photoionization model is used to fit all epochs at all frequencies simultaneously. The differences between the radio flux density predictions and the observed values show some time-independent residuals of typically 1 %. A possible explanation is inaccuracies in the radio flux scale of Baars et al. We propose an adjustment to the flux density scale of the primary radio flux calibrator 3C286, based on the Cloudy model of NGC7027. We also calculate precise flux densities for NGC7027 for all standard continuum bands used at the VLA, as well as for some new 30GHz experiments.Comment: submitted to the Astrophysical Journa

Provision of artificial shelter on beaches is associated with improved shorebird fledging success

Artificial chick shelters might improve productivity of beach-nesting birds threatened by anthropogenic disturbance. We investigated the efficacy of three different chick shelter designs against four criteria: accessibility to chicks over time, thermal insulation, conspicuousness to beach-goers, and practicality (cost and ease of transport). One design (‘A-frame’) was selected because it offered the greatest thermal insulation, was the least conspicuous, most cost effective, and performed equally well in terms of accessibility. We deployed these artificial shelters on Hooded Plover Thinornis rubricollis territories where broods were present (n 5 11), and compared the behaviour and survival rate of chicks to that at control sites (n 5 10). We were unable to discern any difference in the behaviour of broods when artificial shelters were available. However, the survival rate of chicks to fledging was 71.8% higher where an artificial shelter was provided (n 5 21 broods). This was validated by analysing data from a larger sample of broods monitored as part of an active volunteer-based management programme; shelters conferred a 42.8%increase in survival to fledging (n 5 81 broods). Thus, artificial shelters have the potential to increase survival rates of threatened shorebird chicks, though the mechanisms through which survival is increased require further investigation

Aromatic Hydrocarbons, Diamonds, and Fullerenes in Interstellar Space: Puzzles to be Solved by Laboratory and Theoretical Astrochemistry

New research is presented, and previous research is reviewed, on the emission and absorption of interstellar aromatic hydrocarbons. Emission from aromatic hydrocarbons dominate the mid-infrared emission of many galaxies, including our own Milky Way galaxy. Only recently have aromatic hydrocarbons been observed in absorption in the interstellar medium, along lines of sight with high column densities of interstellar gas and dust. Much work on interstellar aromatics has been done, with astronomical observations and laboratory and theoretical astrochemistry. In many cases the predictions of laboratory and theoretical work are confirmed by astronomical observations, but in other cases clear discrepancies exist which provide problems to be solved by a combination of astronomical observations, laboratory studies, and theoretical studies. The emphasis of this paper will be on current outstanding puzzles concerning aromatic hydrocarbons which require further laboratory and theoretical astrochemistry to resolve. This paper will also touch on related topics where laboratory and theoretical astrochemistry studies are needed to explain astrophysical observations, such as a possible absorption feature due to interstellar "diamonds" and the search for fullerenes in space.Comment: Spectrochimica Acta A, Feb. 2001, in press. 33 pages including 11 postscript figures, AASTeX format. Full postscript paper also available at http://www.astronomy.ohio-state.edu/~sellgren/saa.htm

HIFI observations of warm gas in DR21: Shock versus radiative heating

The molecular gas in the DR21 massive star formation region is known to be affected by the strong UV field from the central star cluster and by a fast outflow creating a bright shock. The relative contribution of both heating mechanisms is the matter of a long debate. By better sampling the excitation ladder of various tracers we provide a quantitative distinction between the different heating mechanisms. HIFI observations of mid-J transitions of CO and HCO+ isotopes allow us to bridge the gap in excitation energies between observations from the ground, characterizing the cooler gas, and existing ISO LWS spectra, constraining the properties of the hot gas. Comparing the detailed line profiles allows to identify the physical structure of the different components. In spite of the known shock-excitation of H2 and the clearly visible strong outflow, we find that the emission of all lines up to > 2 THz can be explained by purely radiative heating of the material. However, the new Herschel/HIFI observations reveal two types of excitation conditions. We find hot and dense clumps close to the central cluster, probably dynamically affected by the outflow, and a more widespread distribution of cooler, but nevertheless dense, molecular clumps.Comment: Accepted for publication by A&

The correlation of RNase A enzymatic activity with the changes in the distance between Nepsilon2-His12 and N delta1-His119 upon addition of stabilizing and destabilizing salts.

The effect of stabilizing and destabilizing salts on the catalytic behavior of ribonuclease A (RNase A) was investigated at pH 7.5 and 25 degrees C, using spectrophotometric, viscometric and molecular dynamic methods. The changes in the distance between N(epsilon2) of His(12) and N(delta1) of His(119) at the catalytic center of RNase A upon the addition of sodium sulfate, sodium hydrogen sulfate and sodium thiocyanate were evaluated by molecular dynamic methods. The compactness and expansion in terms of Stokes radius of RNase A upon the addition of sulfate ions as kosmotropic salts, and thiocyanate ion as a chaotropic salt, were estimated by viscometric measurements. Enzyme activity was measured using cytidine 2', 3'-cyclic monophosphate as a substrate. The results from the measurements of distances between N(epsilon2) of His(12) and N(delta1) of His(119) and Stokes radius suggest (i) that the presence of sulfate ions decreases the distance between the catalytic His residues and increases the globular compactness, and (ii) that there is an expansion of the enzyme surface as well as elongation of the catalytic center in the presence of thiocyanate ion. These findings are in agreement with activity measurements

Water in low-mass star-forming regions with Herschel: HIFI spectroscopy of NGC1333

'Water In Star-forming regions with Herschel' (WISH) is a key programme dedicated to studying the role of water and related species during the star-formation process and constraining the physical and chemical properties of young stellar objects. The Heterodyne Instrument for the Far-Infrared (HIFI) on the Herschel Space Observatory observed three deeply embedded protostars in the low-mass star-forming region NGC1333 in several H2-16O, H2-18O, and CO transitions. Line profiles are resolved for five H16O transitions in each source, revealing them to be surprisingly complex. The line profiles are decomposed into broad (>20 km/s), medium-broad (~5-10 km/s), and narrow (<5 km/s) components. The H2-18O emission is only detected in broad 1_10-1_01 lines (>20 km/s), indicating that its physical origin is the same as for the broad H2-16O component. In one of the sources, IRAS4A, an inverse P Cygni profile is observed, a clear sign of infall in the envelope. From the line profiles alone, it is clear that the bulk of emission arises from shocks, both on small (<1000 AU) and large scales along the outflow cavity walls (~10 000 AU). The H2O line profiles are compared to CO line profiles to constrain the H2O abundance as a function of velocity within these shocked regions. The H2O/CO abundance ratios are measured to be in the range of ~0.1-1, corresponding to H2O abundances of ~10-5-10-4 with respect to H2. Approximately 5-10% of the gas is hot enough for all oxygen to be driven into water in warm post-shock gas, mostly at high velocities.Comment: Accepted for publication in the A&A HIFI special issu

Functional Role of Glutamine 28 and Arginine 39 in Double Stranded RNA Cleavage by Human Pancreatic Ribonuclease

Human pancreatic ribonuclease (HPR), a member of RNase A superfamily, has a high activity on double stranded (ds) RNA. By virtue of this activity HPR appears to be involved in the host-defense against pathogenic viruses. To delineate the mechanism of dsRNA cleavage by HPR, we have investigated the role of glutamine 28 and arginine 39 of HPR in its activity on dsRNA. A non-basic residue glycine 38, earlier shown to be important for dsRNA cleavage by HPR was also included in the study in the context of glutamine 28 and arginine 39. Nine variants of HPR respectively containing Q28A, Q28L, R39A, G38D, Q28A/R39A, Q28L/R39A, Q28A/G38D, R39A/G38D and Q28A/G38D/R39A mutations were generated and functionally characterized. The far-UV CD-spectral analysis revealed all variants, except R39A, to have structures similar to that of HPR. The catalytic activity of all HPR variants on single stranded RNA substrate was similar to that of HPR, whereas on dsRNA, the catalytic efficiency of all single residue variants, except for the Q28L, was significantly reduced. The dsRNA cleavage activity of R39A/G38D and Q28A/G38D/R39A variants was most drastically reduced to 4% of that of HPR. The variants having reduced dsRNA cleavage activity also had reduction in their dsDNA melting activity and thermal stability. Our results indicate that in HPR both glutamine 28 and arginine 39 are important for the cleavage of dsRNA. Although these residues are not directly involved in catalysis, both arginine 39 and glutamine 28 appear to be facilitating a productive substrate-enzyme interaction during the dsRNA cleavage by HPR