Genomes, expression profiles, and diversity of mitochondria of the White-footed Deermouse Peromyscus leucopus, reservoir of Lyme disease and other zoonoses.

The cricetine rodents Peromyscus leucopus and P. maniculatus are key reservoirs for several zoonotic diseases in North America. We determined the complete circular mitochondrial genome sequences of representatives of 3 different stock colonies of P. leucopus, one stock colony of P. maniculatus and two wild populations of P. leucopus. The genomes were syntenic with that of the murids Mus musculus and Rattus norvegicus. Phylogenetic analysis confirmed that these two Peromyscus species are sister taxa in a clade with P. polionotus and also uncovered a distinction between P. leucopus populations in the eastern and the central United States. In one P. leucopus lineage four extended regions of mitochondrial pseudogenes were identified in the nuclear genome. RNA-seq analysis revealed transcription of the entire genome and differences from controls in the expression profiles of mitochondrial genes in the blood, but not in liver or brain, of animals infected with the zoonotic pathogen Borrelia hermsii. PCR and sequencing of the D-loop of the mitochondrion identified 32 different haplotypes among 118 wild P. leucopus at a Connecticut field site. These findings help to further establish P. leucopus as a model organism for studies of emerging infectious diseases, ecology, and in other disciplines

Searching for an Intermediate Mass Black Hole in the Blue Compact Dwarf galaxy MRK 996

The possibility is explored that accretion on an intermediate mass black hole contributes to the ionisation of the interstellar medium of the Compact Blue Dwarf galaxy MRK996. Chandra observations set tight upper limits (99.7 per cent confidence level) in both the X-ray luminosity of the posited AGN, Lx(2-10keV)<3e40erg/s, and the black hole mass, <1e4/\lambda Msolar, where \lambda, is the Eddington ratio. The X-ray luminosity upper limit is insufficient to explain the high ionisation line [OIV]25.89\mu m, which is observed in the mid-infrared spectrum of the MRK996 and is proposed as evidence for AGN activity. This indicates that shocks associated with supernovae explosions and winds of young stars must be responsible for this line. It is also found that the properties of the diffuse X-ray emission of MRK996 are consistent with this scenario, thereby providing direct evidence for shocks that heat the galaxy's interstellar medium and contribute to its ionisation.Comment: Submitted to MNRA

Microsporidia-nematode associations in methane seeps reveal basal fungal parasitism in the deep sea

The deep sea is Earth's largest habitat but little is known about the nature of deep-sea parasitism. In contrast to a few characterized cases of bacterial and protistan parasites, the existence and biological significance of deep-sea parasitic fungi is yet to be understood. Here we report the discovery of a fungus-related parasitic microsporidium, Nematocenator marisprofundi n. gen. n. sp. that infects benthic nematodes at methane seeps on the Pacific Ocean floor. This infection is species-specific and has been temporally and spatially stable over 2 years of sampling, indicating an ecologically consistent host-parasite interaction. A high distribution of spores in the reproductive tracts of infected males and females and their absence from host nematodes' intestines suggests a sexual transmission strategy in contrast to the fecal-oral transmission of most microsporidia. N. marisprofundi targets the host's body wall muscles causing cell lysis, and in severe infection even muscle filament degradation. Phylogenetic analyses placed N. marisprofundi in a novel and basal clade not closely related to any described microsporidia clade, suggesting either that microsporidia-nematode parasitism occurred early in microsporidia evolution or that host specialization occurred late in an ancient deep-sea microsporidian lineage. Our findings reveal that methane seeps support complex ecosystems involving interkingdom interactions between bacteria, nematodes, and parasitic fungi and that microsporidia parasitism exists also in the deep-sea biosphere

Learning From Early Attempts to Generalize Darwinian Principles to Social Evolution

Copyright University of Hertfordshire &amp; author.Evolutionary psychology places the human psyche in the context of evolution, and addresses the Darwinian processes involved, particularly at the level of genetic evolution. A logically separate and potentially complementary argument is to consider the application of Darwinian principles not only to genes but also to social entities and processes. This idea of extending Darwinian principles was suggested by Darwin himself. Attempts to do this appeared as early as the 1870s and proliferated until the early twentieth century. But such ideas remained dormant in the social sciences from the 1920s until after the Second World War. Some lessons can be learned from this earlier period, particularly concerning the problem of specifying the social units of selection or replication

Technical Note: Characterization and correction of gradient nonlinearity induced distortion on a 1.0 T open bore MRâ SIM

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135020/1/mp0245.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/135020/2/mp0245_am.pd

The local star-formation rate density: assessing calibrations using [OII], Ha and UV luminosities

We explore the use of simple star-formation rate (SFR) indicators (such as may be used in high-redshift galaxy surveys) in the local Universe using [OII], Ha, and u-band luminosities from the deeper 275 deg^2 Stripe 82 subsample of the Sloan Digital Sky Survey (SDSS) coupled with UV data from the Galaxy Evolution EXplorer satellite (GALEX). We examine the consistency of such methods using the star-formation rate density (SFRD) as a function of stellar mass in this local volume, and quantify the accuracy of corrections for dust and metallicity on the various indicators. Rest-frame u-band promises to be a particularly good SFR estimator for high redshift studies since it does not require a particularly large or sensitive extinction correction, yet yields results broadly consistent with more observationally expensive methods. We suggest that the [OII]-derived SFR, commonly used at higher redshifts (z~1), can be used to reliably estimate SFRs for ensembles of galaxies, but for high mass galaxies (log(M*/Msun)>10), a larger correction than is typically used is required to compensate for the effects of metallicity dependence and dust extinction. We provide a new empirical mass-dependent correction for the [OII]-SFR.Comment: 22 pages, 16 figures. This version corrects typos in equations 2, 7, and 9 of the published version, as described in the MNRAS Erratum. Published results are unaffected. A simple piece of IDL Code for applying the mass-dependent correction to [OII] SFR available from http://astro.uwaterloo.ca/~dgilbank/data/corroii.pr

Integral field spectroscopy of nitrogen overabundant blue compact dwarf galaxies

We study the spatial distribution of the physical properties and of oxygen and nitrogen abundances in three Blue Compact Dwarf Galaxiess (HS 0128+2832, HS 0837+4717 and Mrk 930) with a reported excess of N/O in order to investigate the nature of this excess and, particularly, if it is associated with Wolf-Rayet (WR) stars We have observed these BCDs by using PMAS integral field spectroscopy in the optical spectral range (3700 - 6900 {\AA}), mapping their physical-chemical properties, using both the direct method and appropriate strong-line methods. We make a statistical analysis of the resulting distributions and we compare them with the integrated properties of the galaxies. Our results indicate that outer parts of the three galaxies are placed on the "AGN-zone" of the [NII]/H{\alpha} vs. [OIII]/H{\beta} diagnostic diagram most likely due to a high N/O combined with the excitation structure in these regions. From the statistical analysis, it is assumed that a certain property can be considered as spatially homogeneous (or uniform) if a normal gaussian function fits its distribution in several regions of the galaxy. Moreover, a disagreement between the integrated properties and the mean values of the distribution usually appears when a gaussian does not fit the corresponding distribution. We find that for Mrk 930, the uniformity is found for all parameters, except for electron density and reddening. The rotation curve together with the H{\alpha} map and UV images, reveal a perturbed morphology and possible interacting processes. The N/O is found to be constant in the three studied objects at spatial scales of the order of several kpc so we conclude that the number of WR stars estimated from spectroscopy is not sufficient to pollute the ISM and to produce the observed N/O excess in these objectsComment: 17 pages, 14 figures, accepted for publication in Astronomy & Astrophysic