Quantifying methane vibrational and rotational temperature with Raman scattering

This work describes the theoretical basis and implementation of the measurement of vibrational (T vib) and rotational (T rot) temperatures in CH4 by fitting spontaneous Raman scattering spectra in the Pentad region. This method could be applied for thermal equilibrium temperature measurements applications, e.g. in combustion, or vibrational-rotational non-equilibrium applications, such as in plasma chemistry. The method of calculating these temperatures is validated against known temperature thermal equilibrium spectra up to 860 K from published data, giving an estimated relative error of 10%. This demonstrates that both the calculated stick spectrum and the algorithm to determine T vib and T rot for CH4 is robust to 860 K, but we expect it is valid to 1500 K. Additionally, a number of non-equilibrium spectra generated with a pulsed microwave plasma are fitted to find T vib and T rot, further demonstrating the applicability of this method in fitting non-equilibrium spectra.</p

Snake Venom Disintegrins and Cell Migration

Cell migration is a key process for the defense of pluricellular organisms against pathogens, and it involves a set of surface receptors acting in an ordered fashion to contribute directionality to the movement. Among these receptors are the integrins, which connect the cell cytoskeleton to the extracellular matrix components, thus playing a central role in cell migration. Integrin clustering at focal adhesions drives actin polymerization along the cell leading edge, resulting in polarity of cell movement. Therefore, small integrin-binding proteins such as the snake venom disintegrins that inhibit integrin-mediated cell adhesion are expected to inhibit cell migration. Here we review the current knowledge on disintegrin and disintegrin-like protein effects on cell migration and their potential use as pharmacological tools in anti-inflammatory therapy as well as in inhibition of metastatic invasion

Vicrostatin – An Anti-Invasive Multi-Integrin Targeting Chimeric Disintegrin with Tumor Anti-Angiogenic and Pro-Apoptotic Activities

Similar to other integrin-targeting strategies, disintegrins have previously shown good efficacy in animal cancer models with favorable pharmacological attributes and translational potential. Nonetheless, these polypeptides are notoriously difficult to produce recombinantly due to their particular structure requiring the correct pairing of multiple disulfide bonds for biological activity. Here, we show that a sequence-engineered disintegrin (called vicrostatin or VCN) can be reliably produced in large scale amounts directly in the oxidative cytoplasm of Origami B E. coli. Through multiple integrin ligation (i.e., αvβ3, αvβ5, and α5β1), VCN targets both endothelial and cancer cells significantly inhibiting their motility through a reconstituted basement membrane. Interestingly, in a manner distinct from other integrin ligands but reminiscent of some ECM-derived endogenous anti-angiogenic fragments previously described in the literature, VCN profoundly disrupts the actin cytoskeleton of endothelial cells (EC) inducing a rapid disassembly of stress fibers and actin reorganization, ultimately interfering with EC's ability to invade and form tubes (tubulogenesis). Moreover, here we show for the first time that the addition of a disintegrin to tubulogenic EC sandwiched in vitro between two Matrigel layers negatively impacts their survival despite the presence of abundant haptotactic cues. A liposomal formulation of VCN (LVCN) was further evaluated in vivo in two animal cancer models with different growth characteristics. Our data demonstrate that LVCN is well tolerated while exerting a significant delay in tumor growth and an increase in the survival of treated animals. These results can be partially explained by potent tumor anti-angiogenic and pro-apoptotic effects induced by LVCN

Strategies to inhibit tumour associated integrin receptors: rationale for dual and multi-antagonists

YesThe integrins are a family of 24 heterodimeric transmembrane cell surface receptors. Involvement in cell attachment to the extracellular matrix, motility, and proliferation identifies integrins as therapeutic targets in cancer and associated conditions; thrombosis, angiogenesis and osteoporosis. The most reported strategy for drug development is synthesis of an agent that is highly selective for a single integrin receptor. However, the ability of cancer cells to change their integrin repertoire in response to drug treatment renders this approach vulnerable to the development of resistance and paradoxical promotion of tumor growth. Here, we review progress towards development of antagonists targeting two or more members of the RGD-binding integrins, notably αvβ3, αvβ5, αvβ6, αvβ8, α5β1, and αIIbβ3, as anticancer therapeutics

The sectoral trade losses from financial crises

International audienceAbstract The “Great Trade Collapse” triggered by the 2008-2009 crisis calls for a careful assessment of the trade losses from financial crises. We adopt a more detailed perspective by looking at the response of different types of trade (i.e. consumption, intermediate, capital goods, and business services) following various types of financial crises (i.e. debt, banking, and currency crises) in 41 emerging markets. Estimations performed in the 1980-2019 period using a combination of impact assessment and local projections to capture a causal dynamic effect running from financial crises to the trade activity show that the collapse of total trade is long-lasting and mainly driven by the fall of intermediate goods and to some extent capital goods, while trade in consumption goods and business services is more resilient to crises. Therefore, financial crises could lead to considerable disruption of global value chains, as observed during the Global Financial Crisis (GFC), and easily spill over from one country to another through trade linkages. The examination of heterogeneity reveals that total and sectoral trade is more severely impacted in countries with a lower share of manufacturing exports, less diversified exported products, and trading partners, with lower demand from trading partners and when associated with a deterioration of the domestic and external financial conditions and sudden stops. By contributing to the understanding of the trade effects of financial crises, our analysis provides insightful support for the design and implementation of policies aimed at coping with these effects

Dynamics of microparticles in vacuum breakdown: Cranberg’s scenario updated by numerical modeling

International audienceMicroparticles (MP) and thermofield emission in vacuum are mainly caused by the roughness present at the surface of electrodes holding a high voltage. They can act as a trigger for breakdown, especially under high vacuum. This theoretical study discusses the interactions between one MP and the thermofield emission electron current as well as the consequences on the MP’s transit. Starting from Cranberg’s assumptions, new phenomena have been taken into account such as MP charge variation due to the secondary electron emission induced by energetic electron bombardment. Hence, the present model can be solved only numerically. Four scenarios have been identified based on the results, depending on the electron emission current from the cathode roughness (tip) and the size of the MP released at the anode, namely (i) one way; (ii) back and forth; (iii) oscillation; and (iv) vaporization. A crash study of the MP on the cathode shows that the electron emission can decrease if the MP covers the thermoemissive tip, i.e., if the MP is larger than the tip size—a phenomenon often called “conditioning”—and helping to increase the voltage holding in vacuum without breakdown