Pharmacological interventions in the treatment of the acute effects of cannabis: a systematic review of literature

<p>Abstract</p> <p>Background</p> <p>Cannabis intoxication is related to a number of physical and mental health risks with ensuing social costs. However, little attention has been given to the investigation of possible pharmacological interactions in this condition.</p> <p>Objective</p> <p>To review the available scientific literature concerning pharmacological interventions for the treatment of the acute effects of cannabis.</p> <p>Methods</p> <p>A search was performed on the <it>Pubmed</it>, <it>Lilacs</it>, and <it>Scielo </it>online databases by combining the terms <it>cannabis, intoxication, psychosis, anxiety</it>, and <it>treatment</it>. The articles selected from this search had their reference lists checked for additional publications related to the topic of the review.</p> <p>Results</p> <p>The reviewed articles consisted of case reports and controlled clinical trials and are presented according to interventions targeting the physiological, psychiatric, and cognitive symptoms provoked by cannabis. The pharmacological interventions reported in these studies include: beta-blockers, antiarrhythmic agents, antagonists of CB-1 and GABA-benzodiazepine receptors, antipsychotics, and cannabidiol.</p> <p>Conclusion</p> <p>Although scarce, the evidence on pharmacological interventions for the management of cannabis intoxication suggests that propanolol and rimonabant are the most effective compounds currently available to treat the physiological and subjective effects of the drug. Further studies are necessary to establish the real effectiveness of these two medications, as well as the effectiveness of other candidate compounds to counteract the effects of cannabis intoxication, such as cannabidiol and flumazenil.</p

Assessing the correlation between FDG PET findings of IDC breast carcinoma and histopathology of coexisting ductal carcinoma in-situ

Background: Ductal carcinoma in-situ (DCIS) often coexists with invasive ductal carcinoma (IDC) of the breast. DCIS is considered as a non-obligate precursor of IDC when both coexist. 18F-fluorodeoxyglucose positron emission tomography/computed tomography ([18F]FDG PET/CT) imaging is commonly used in the staging and follow-up assessment of breast cancer. In this study, we aimed to assess if there is any correlation between primary tumor PET and histopathology findings and histopathological features of the coexisting DCIS. Material and methods: FDG PET/CT images and histopathology results of the patients with newly diagnosed breast cancer (IDC) with coexisting DCIS were analyzed in this retrospective study. The grade and size of the primary tumor and histopathological features of the coexisting DCIS (nuclear grade and architectural pattern) were obtained from the postoperative histopathology results. Maximum standardized uptake values (SUV: SUVmax and SULmax) of the primary tumor normalized by weight and lean body mass were measured. Statistical analysis was performed to assess the correlation between various parameters of IDC and DCIS. Results: This study included sixty-two (62) patients with IDC-DCIS. Primary tumor grade was significantly correlated and associated with the nuclear grade of the coexisting DCIS (polychoric correlation r = 0.736, and Fisher exact test, PV &lt; 0.001, respectively). Primary tumor SUV was not correlated with the nuclear grade and architectural pattern of the coexisting DCIS (polyserial correlation r = 0.172, PV = 0.155, and Point Bi-Serial correlation r = –0.009, PV = 0.955, respectively). Median primary tumor size was marginally significantly different among DCIS nuclear grades but it was not significantly different in comedo and non-comedo cases (Kruskal-Wallis test PV = 0.053, and Mann-Whitney U test PV = 0.890, respectively). Conclusions: Primary tumor grade is correlated with the nuclear grade of the coexisting DCIS. SUV of primary tumor does not seem to be correlated with the histopathological features of coexisting DCIS (nuclear grade and architectural pattern) but this may be further studied in a larger number of patients

A nanocommunication system for endocrine diseases

Nanotechnology is a newand very promising area of research which will allow several new applications to be created in different fields, such as, biological, medical, environmental, military, agricultural, industrial and consumer goods. This paper focuses specifically on nanocommunications, which will allow interconnected devices, at the nano-scale, to achieve collaborative tasks, greatly changing the paradigm in the fields described. Molecular communication is a new communication paradigm which allows nanomachines to exchange information using molecules as carrier. This is the most promising nanocommunication method within nanonetworks, since it can use bio-inspired techniques, inherit from studied biological systems, which makes the connection of biologic and man-made systems a easier process. At this point, the biggest challenges in these type of nanocommunication are to establish feasible and reliable techniques that will allow information to be encoded, and mechanisms that ensure a molecular communication between different nodes. This paper focus on creating concepts and techniques to tackle these challenges, and establishing new foundations on which future work can be developed. The created concepts and techniques are then applied in an envisioned medical application, which is based on a molecular nanonetwork deployed inside the Human body. The goal of this medical application is to automatously monitor endocrine diseases using the benefits of nanonetworks, which in turn connects with the internet, thus creating a Internet of NanoThings system. The concepts and techniques developed are evaluated by performing several simulations and comparing with other researches, and the results and discussions are presented on the later sections of this paper

Mitochondrial carrier homolog 1 (Mtch1) antibodies in neuro-Behçet's disease

Cataloged from PDF version of article.Efforts for the identification of diagnostic autoantibodies for neuro-Behcet's disease (NBD) have failed. Screening of NBD patients' sera with protein macroarray identified mitochondrial carrier homolog 1 (Mtch1), an apoptosis-related protein, as a potential autoantigen. ELISA studies showed serum Mtch1 antibodies in 68 of 144 BD patients with or without neurological involvement and in 4 of 168 controls corresponding to a sensitivity of 47.2% and specificity of 97.6%. Mtch1 antibody positive NBD patients had more attacks, increased disability and lower serum nucleosome levels. Mtch1 antibody might be involved in pathogenic mechanisms of NBD rather than being a coincidental byproduct of autoinflammation. © 2013 Elsevier B.V

Empirical evaluation of microtremor H/V spectral ratio

The objective of this work is to perform a purely empirical assessment of the actual capabilities of the horizontal-to-vertical (H/V) spectral ratio technique to provide reliable and relevant information concerning site conditions and/or site amplification. This objective has been tackled through the homogeneous (re)processing of a large volume of earthquakes and ambient noise data recorded by different research teams in more than 200 sites located mainly in Europe, but also in the Caribbean and in Tehran. The original recordings were first gathered in a specific database with information on both the sites and recorded events. Then, for all sites close to an instrumented reference, average site-to-reference spectral ratios (“spectral ratio method” (SSR)) were derived in a homogeneous way (window selection, smoothing, signal-to-noise ratio threshold, averaging), as well as H/V ratios (“HVSRE–RF”) on earthquake recordings. H/V ratios were also obtained from noise recordings at each site (either specific measurements, or extracted from pre- or post-event noise windows). The spectral curves resulting from these three techniques were estimated reliable for a subset of 104 sites, and were thus compared in terms of fundamental frequency, amplitude and amplification bandwidth, exhibiting agreements and disagreements, for which interpretations are looked for in relation with characteristics of site conditions. The first important result consists in the very good agreement between fundamental frequencies obtained with either technique, observed for 81% of the analyzed sites. A significant part of the disagreements correspond to thick, low frequency, continental sites where natural noise level is often very low and H/V noise ratios do not exhibit any clear peak. The second important result is the absence of correlation between H/V peak amplitude and the actual site amplification measured on site-to-reference spectral ratios. There are, however, two statistically significant results about the amplitude of the H/V curve: the peak amplitude may be considered as a lower bound estimate of the actual amplification indicated by SSR (it is smaller for 79% of the 104 investigated sites), and, from another point of view, the difference in amplitude exhibits a questioning correlation with the geometrical characteristics of the sediment/basement interface: large SSR/HV differences might thus help to detect the existence of significant 2D or 3D effects.Published75-1084.1. Metodologie sismologiche per l'ingegneria sismicaJCR Journalreserve

Gas seepage and seismogenic structures along the North Anatolian Fault in the eastern Sea of Marmara

We carried out a combined geophysical and gas-geochemical survey on an active fault strand along the North Anatolian Fault (NAF) system in the Gulf of İzmit (eastern Sea of Marmara), providing for the first time in this area data on the distribution of methane (CH4) and other gases dissolved in the bottom seawater, as well as the CH4isotopic composition. Based on high-resolution morphobathymetric data and chirp-sonar seismic reflection profiles we selected three areas with different tectonic features associated to the NAF system, where we performed visual and instrumental seafloor inspections, including in situ measurements of dissolved CH4, and sampling of the bottom water. Starting from background values of 2–10 nM, methane concentration in the bottom seawater increases abruptly up to 20 nM over the main NAF trace. CH4 concentration peaks up to ∼120 nM were detected above mounds related probably to gas and fluids expulsion. Methane is microbial (δ13CCH4: −67.3 and −76‰ versus VPDB), and was found mainly associated with pre-Holocene deposits topped by a 10–20 m thick draping of marine mud. The correlation between tectonic structures and gas-seepages at the seafloor suggests that the NAF in the Gulf of İzmit could represent a key site for long-term combined monitoring of fluid exhalations and seismicity to assess their potential as earthquake precursors

Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds

Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques suffer from limited control over pore interconnectivity, density and size, which leads to inefficient nutrient and oxygen transport to cells embedded in the scaffolds. Here, we demonstrated an innovative approach to develop a new platform for tissue engineered constructs using live bacteria as sacrificial porogens. E.coli were patterned and cultured in an interconnected three-dimensional (3D) hydrogel network. The growing bacteria created interconnected micropores and microchannels. Then, the scafold was decellularized, and bacteria were eliminated from the scaffold through lysing and washing steps. This 3D porous network method combined with bioprinting has the potential to be broadly applicable and compatible with tissue specific applications allowing seeding of stem cells and other cell types

Statistical Modeling of Single Target Cell Encapsulation

High throughput drop-on-demand systems for separation and encapsulation of individual target cells from heterogeneous mixtures of multiple cell types is an emerging method in biotechnology that has broad applications in tissue engineering and regenerative medicine, genomics, and cryobiology. However, cell encapsulation in droplets is a random process that is hard to control. Statistical models can provide an understanding of the underlying processes and estimation of the relevant parameters, and enable reliable and repeatable control over the encapsulation of cells in droplets during the isolation process with high confidence level. We have modeled and experimentally verified a microdroplet-based cell encapsulation process for various combinations of cell loading and target cell concentrations. Here, we explain theoretically and validate experimentally a model to isolate and pattern single target cells from heterogeneous mixtures without using complex peripheral systems.Wallace H. Coulter Foundation (Young Investigator in Bioengineering Award)National Institutes of Health (U.S.) (Grant R01AI081534)National Institutes of Health (U.S.) (Grant R21AI087107

A chemical model for the atmosphere of hot Jupiters

Our purpose is to release a chemical network, and the associated rate coefficients, developed for the temperature and pressure range relevant to hot Jupiters atmospheres. Using this network, we study the vertical atmospheric composition of the two hot Jupiters (HD209458b, HD189733b) with a model that includes photolyses and vertical mixing and we produce synthetic spectra. The chemical scheme is derived from applied combustion models that have been methodically validated over a range of temperatures and pressures typical of the atmospheric layers influencing the observations of hot Jupiters. We compare the predictions obtained from this scheme with equilibrium calculations, with different schemes available in the literature that contain N-bearing species and with previously published photochemical models. Compared to other chemical schemes that were not subjected to the same systematic validation, we find significant differences whenever non-equilibrium processes take place. The deviations from the equilibrium, and thus the sensitivity to the network, are more important for HD189733b, as we assume a cooler atmosphere than for HD209458b. We found that the abundances of NH3 and HCN can vary by two orders of magnitude depending on the network, demonstrating the importance of comprehensive experimental validation. A spectral feature of NH3 at 10.5$\mu$m is sensitive to these abundance variations and thus to the chemical scheme. Due to the influence of the kinetics, we recommend the use of a validated scheme to model the chemistry of exoplanet atmospheres. Our network is robust for temperatures within 300-2500K and pressures from 10mbar up to a few hundreds of bars, for species made of C,H,O,N. It is validated for species up to 2 carbon atoms and for the main nitrogen species.Comment: 20 pages, 10 figures. Accepted for publication in Astronomy & Astrophysic