Efeito das dimensões do espécime na resistência à flexão de uma resina composta

PUORPOSE: The aim of this study was to evaluate the effect of specimen dimensions on the flexural strength of a composite resin (Heliomolar RO). METHODS: The different dimensions tested - length x width x height (mm) were: 25x2x4; 25x2x2 (ISO 4049); 15x2x2; 10x2x2 and 10x2x1. Light-curing was performed at 600 mW/cm² for 40s, three times along the 25mm specimens, twice along 15mm specimen and once for the 10mm specimens. Specimens of all dimensions were light-cured on both surfaces, or only on one side. In the latter, the load was applied on the irradiated side or on the opposite one. RESULTS: It was shown that the flexural strength was not affected by specimen length. When light-curing was performed on both surfaces, similar flexural strength values were obtained for any dimension. Despite the number of irradiated surfaces, specimens with a height of 1mm also obtained similar values. On the contrary, specimens with a height of 4mm, exposed only on one surface, reached the lowest strength. CONCLUSION: The use of specimens with lower dimensions can lead to flexural strength values similar to the ones obtained with standardized specimen (ISO 4049), with the advantage of demanding less amount of material and being less time consuming.OBJETIVO: A finalidade da presente investigação foi avaliar a influência das dimensões dos corpos-de-prova (cp) e da superfície de irradiação na resistência à flexão de uma resina composta (Heliomolar RO). MÉTODOS: As diversas dimensões adotadas - comprimento x largura x altura (mm) foram: 25x2x4; 25x2x2 (ISO 4049); 15x2x2; 10x2x2; 10x2x1. A fotoativação foi realizada por 40 segundos a 600mW/cm²; três vezes ao longo dos cp de 25mm, duas vezes ao longo dos cp de 15mm e uma vez nos cp de 10mm de comprimento. Os corpos-de-prova de todas as dimensões foram ativados em ambas as superfícies, ou apenas em uma; neste caso a carga de ruptura era aplicada na face de irradiação ou na oposta. RESULTADOS: Foi mostrado que a resistência à flexão não foi afetada pelo comprimento dos cp. Com a fotoativação em ambas as superfícies, as resistências obtidas eram semelhantes nos diversos comprimentos. Independentemente do número de faces irradiadas, os cp com 1mm de altura apresentaram resistências semelhantes. Contudo, cp com 4mm de altura e irradiados apenas por um lado, alcançaram os menores valores. CONCLUSÃO: Com o emprego de cp com dimensões menores são alcançados valores de resistência à flexão semelhante aos obtidos com cp padronizados (ISO 4049), com a vantagem de menor demanda de material e menor consumo de tempo (o menor comprimento dos cp requer apenas uma ativação por lado)

Influência da manipulação na elasticidade de siliconas de adição em consistência de massa

The handling of vinyl polysiloxane (addition silicone) impression putties with latex gloves is said to interfere with the setting of these impression materials. The aim of this study is to evaluate the effect of handling techniques on the setting of vinyl polysiloxane impression putties using several types of gloves. The setting of these materials was evaluated by means of an elasticimeter. Four vinyl polysiloxane putty impression materials and five brands of gloves (one made of vinyl, one of synthetic rubber, and three of natural rubber) were studied. Based on the type of glove, they were previously washed or not, and a spatula was used or not for initial mixing (before handmixing). The vinyl, the synthetic and one of the natural rubber gloves did not require the previous washing procedure and/or the use of a spatula for initial mixing. Two other natural rubber gloves - depending on the silicone -, showed satisfactory results only when the initial mixing was performed with a spatula. It was concluded that setting inhibition depends on the kind of vinyl polysiloxane impression material and the kind of gloves used, but when the initial mixing was performed with the spatula this setting inhibition was overcome. The results of this study also showed that it is possible to associate cross-contamination control and satisfactory performance of addition silicone putty materials. When doubts arise from the compatibility between vinyl polysiloxane impression putties and gloves, the initial mixing should be performed with a spatula.A finalidade da presente pesquisa foi avaliar a influência na elasticidade da manipulação de silicones de adição em consistência de massa, por meio de várias técnicas, empregando-se luvas. A polimerização dos materiais foi avaliada por meio de um elasticímetro. Ensaiaram-se quatro silicones, empregando cinco marcas de luvas (uma de vinil, uma de borracha sintética e três de borracha natural). Dependendo do tipo de luva, ela foi lavada ou não e ainda foi utilizada ou não uma espátula para a mistura inicial dos silicones. Os resultados demonstraram que no emprego das luvas de vinil, uma das de borracha sintética e uma das de borracha natural não precisaram ser lavadas, nem a utilização de espátula para a mistura inicial foi necessária. Duas luvas de borracha natural não conduziram a resultados satisfatórios com todos os silicones sem que a mistura inicial fosse feita com a espátula mas, com essa prática, todas as luvas foram compatíveis com todos os silicones. Pode-se concluir que, em caso de dúvida sobre a compatibilidade entre tipo de luva e marca de silicone, o emprego de espátula na mistura inicial contorna o problema

Influência do tempo de armazenamento na microinfiltração de restaurações de resina composta: avaliação qualitativa e quantitativa

Objetivou-se verificar a influência do tempo de armazenamento (24 horas ou 3 meses) no selamento marginal de restaurações de classe II e, além disso, verificar a correlação entre dois métodos de obtenção dos resultados nos testes de microinfiltração. Cavidades classe II foram confeccionadas em molares humanos íntegros, sendo as cavidades MO restauradas com ABF experimental (Kuraray Medical Inc.) + Z250 (3M ESPE) e as DO, com Single Bond (3M ESPE) + Z250. Após os diferentes tempos de armazenamento, empregou-se uma metodologia para provocar microinfiltração (ciclagem térmica e corante azul de metileno 0,5% por 4 horas) e os corpos-de-prova foram seccionados em 3 fatias. Três avaliadores calibrados atribuíram escores conforme o grau de infiltração, e também foram realizadas medidas morfométricas com ajuda do software ImageLab 2000. A análise de regressão de Pearson mostrou correlação altamente significante entre os valores obtidos pelos dois métodos de avaliação (r = 0,978, p < 0,001). Constatou-se que o adesivo ABF experimental apresentou menor infiltração marginal em relação ao adesivo Single Bond (p < 0,001), sendo que o tempo de armazenamento não influenciou no comportamento dos adesivos.The aim of this study was to evaluate the influence of storage periods of 24 hours and 3 months on the microleakage of class II cavities. Two methods of assessing microleakage were also compared. Class II cavities were prepared in sound human molars. MO cavities were restored using ABF experimental (Kuraray Medical Inc.) + Z250 composite resin (3M ESPE), and DO cavities were restored using Single Bond (3M ESPE) + Z250. After different storage periods, specimens were thermocycled, immersed in a dye (0.5% methylene blue solution for 4 h) and longitudinally sectioned. Dye penetration was scored according to a 0-4 scale. The extent of microleakage was measured using the ImageLab 2000 program. A statistically significant correlation was verified between both evaluation methods (r = 0.978, p < 0.001). ANOVA revealed a statistically significant difference between the tested adhesive systems regarding microleakage (p < 0.001), although it was not influenced by the different storage periods

An Initial Mass Function for Individual Stars in Galactic Disks: I. Constraining the Shape of the IMF

We derive a semi-empirical galactic initial mass function (IMF) from observational constraints. We assume that the star formation rate in a galaxy can be expressed as the product of the IMF, $\psi (m)$, which is a smooth function of mass $m$ (in units of \msun), and a time- and space-dependent total rate of star formation per unit area of galactic disk. The mass dependence of the proposed IMF is determined by five parameters: the low-mass slope $\gamma$, the high-mass slope $-\Gamma$, the characteristic mass $m_{ch}$ (which is close to the mass $m_{\rm peak}$ at which the IMF turns over), and the lower and upper limits on the mass, $m_l$ (taken to be 0.004) and $m_u$ (taken to be 120). The star formation rate in terms of number of stars per unit area of galactic disk per unit logarithmic mass interval, is proportional to $m^{-\Gamma} \left\{1-\exp\left[{-(m/m_{ch})^{\gamma +\Gamma}}\right]\right\}$, where $\cal N_*$ is the number of stars, $m_l<m<m_u$ is the range of stellar masses. The values of $\gamma$ and \emch are derived from two integral constraints: i) the ratio of the number density of stars in the range $m=0.1-0.6$ to that in the range $m=0.6-0.8$ as inferred from the mass distribution of field stars in the local neighborhood, and ii) the ratio of the number of stars in the range $m=0.08 - 1$ to the number of brown dwarfs in the range $m=0.03-0.08$ in young clusters. The IMF satisfying the above constraints is characterized by the parameters $\gamma=0.51$ and \emch=0.35 (which corresponds to $m_{\rm peak}=0.27$). This IMF agrees quite well with the Chabrier (2005) IMF for the entire mass range over which we have compared with data, but predicts significantly more stars with masses $< 0.03\, M_\odot$; we also compare with other IMFs in current use.Comment: 46 pages, 8 figures, submitted to Ap

Geoeconomic variations in epidemiology, ventilation management, and outcomes in invasively ventilated intensive care unit patients without acute respiratory distress syndrome: a pooled analysis of four observational studies

Background: Geoeconomic variations in epidemiology, the practice of ventilation, and outcome in invasively ventilated intensive care unit (ICU) patients without acute respiratory distress syndrome (ARDS) remain unexplored. In this analysis we aim to address these gaps using individual patient data of four large observational studies. Methods: In this pooled analysis we harmonised individual patient data from the ERICC, LUNG SAFE, PRoVENT, and PRoVENT-iMiC prospective observational studies, which were conducted from June, 2011, to December, 2018, in 534 ICUs in 54 countries. We used the 2016 World Bank classification to define two geoeconomic regions: middle-income countries (MICs) and high-income countries (HICs). ARDS was defined according to the Berlin criteria. Descriptive statistics were used to compare patients in MICs versus HICs. The primary outcome was the use of low tidal volume ventilation (LTVV) for the first 3 days of mechanical ventilation. Secondary outcomes were key ventilation parameters (tidal volume size, positive end-expiratory pressure, fraction of inspired oxygen, peak pressure, plateau pressure, driving pressure, and respiratory rate), patient characteristics, the risk for and actual development of acute respiratory distress syndrome after the first day of ventilation, duration of ventilation, ICU length of stay, and ICU mortality. Findings: Of the 7608 patients included in the original studies, this analysis included 3852 patients without ARDS, of whom 2345 were from MICs and 1507 were from HICs. Patients in MICs were younger, shorter and with a slightly lower body-mass index, more often had diabetes and active cancer, but less often chronic obstructive pulmonary disease and heart failure than patients from HICs. Sequential organ failure assessment scores were similar in MICs and HICs. Use of LTVV in MICs and HICs was comparable (42\ub74% vs 44\ub72%; absolute difference \u20131\ub769 [\u20139\ub758 to 6\ub711] p=0\ub767; data available in 3174 [82%] of 3852 patients). The median applied positive end expiratory pressure was lower in MICs than in HICs (5 [IQR 5\u20138] vs 6 [5\u20138] cm H2O; p=0\ub70011). ICU mortality was higher in MICs than in HICs (30\ub75% vs 19\ub79%; p=0\ub70004; adjusted effect 16\ub741% [95% CI 9\ub752\u201323\ub752]; p&lt;0\ub70001) and was inversely associated with gross domestic product (adjusted odds ratio for a US$10 000 increase per capita 0\ub780 [95% CI 0\ub775\u20130\ub786]; p&lt;0\ub70001). Interpretation: Despite similar disease severity and ventilation management, ICU mortality in patients without ARDS is higher in MICs than in HICs, with a strong association with country-level economic status. Funding: No funding

Relationship between molecular pathogen detection and clinical disease in febrile children across Europe: a multicentre, prospective observational study

BackgroundThe PERFORM study aimed to understand causes of febrile childhood illness by comparing molecular pathogen detection with current clinical practice.MethodsFebrile children and controls were recruited on presentation to hospital in 9 European countries 2016-2020. Each child was assigned a standardized diagnostic category based on retrospective review of local clinical and microbiological data. Subsequently, centralised molecular tests (CMTs) for 19 respiratory and 27 blood pathogens were performed.FindingsOf 4611 febrile children, 643 (14%) were classified as definite bacterial infection (DB), 491 (11%) as definite viral infection (DV), and 3477 (75%) had uncertain aetiology. 1061 controls without infection were recruited. CMTs detected blood bacteria more frequently in DB than DV cases for N. meningitidis (OR: 3.37, 95% CI: 1.92-5.99), S. pneumoniae (OR: 3.89, 95% CI: 2.07-7.59), Group A streptococcus (OR 2.73, 95% CI 1.13-6.09) and E. coli (OR 2.7, 95% CI 1.02-6.71). Respiratory viruses were more common in febrile children than controls, but only influenza A (OR 0.24, 95% CI 0.11-0.46), influenza B (OR 0.12, 95% CI 0.02-0.37) and RSV (OR 0.16, 95% CI: 0.06-0.36) were less common in DB than DV cases. Of 16 blood viruses, enterovirus (OR 0.43, 95% CI 0.23-0.72) and EBV (OR 0.71, 95% CI 0.56-0.90) were detected less often in DB than DV cases. Combined local diagnostics and CMTs respectively detected blood viruses and respiratory viruses in 360 (56%) and 161 (25%) of DB cases, and virus detection ruled-out bacterial infection poorly, with predictive values of 0.64 and 0.68 respectively.InterpretationMost febrile children cannot be conclusively defined as having bacterial or viral infection when molecular tests supplement conventional approaches. Viruses are detected in most patients with bacterial infections, and the clinical value of individual pathogen detection in determining treatment is low. New approaches are needed to help determine which febrile children require antibiotics.FundingEU Horizon 2020 grant 668303

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children

ESTUDO da RETENÇÃO ENTRE RESINA ACRÍLICA PARA BASE DE DENTADURA E DENTES DE RESINA ACRÍLICA

Foi avaliada a resistência de união entre dentes e base de dentadura, ambos de resina acrílica. Os dentes foram incluídos em blocos cilíndricos de resina e, posteriormente, torneados, para se obterem cilindros de 5mm de diâmetro. Sobre a extremidade do dente, era adaptado um padrão de cera de mesmo diâmetro, que, após inclusão na mufla, era substituído por resina acrílica termicamente ativada pelo método de processamento convencional de base de dentadura. Como variáveis, usaram-se duas marcas de dentes, duas fases de inclusão (plástica e borrachóide) e aplicação ou não de detergente e monômero sobre os dentes. Os corpos-de-prova, antes dos ensaios de ruptura por tração, foram armazenados em água destilada, a 37ºC, por 2 semanas. Os resultados mostraram que: marca de dente e fase de condensação não influíram nos resultados; somente o uso de detergente ou de monômero aumenta a retentividade e o seu uso conjunto apresenta efeito acumulativo.An investigation was carried out to evaluate the bonding strength between acrylic teeth and heat-cured denture acrylic resin. The teeth were fixed in resin blocks and lathe milled to a cylinder shape of 5 mm of diameter. A wax pattern with the same diameter was adapted on the top of the cylinder and then invested in a flask. Following the conventional denture base resin procedures, the wax pattern was eliminated and heat-cured resin was packed. The variables were: two acrylic teeth brands; two resin phases (full dough and rubber); cleaning or not of the flask with detergent; application or not of monomer over the teeth before resin packing. After polymerization specimens were stored in water at 37ºC, for 2 weeks. Tests were performed by tensile loads. The results showed that teeth brands and resin phases did not influence data; the use of monomer or detergent increased strengths, and when used together the effects were cumulative