91 research outputs found
Mobility of D atoms on porous amorphous water ice surfaces under interstellar conditions
Aims. The mobility of H atoms on the surface of interstellar dust grains at
low temperature is still a matter of debate. In dense clouds, the hydrogenation
of adsorbed species (i.e., CO), as well as the subsequent deuteration of the
accreted molecules depend on the mobility of H atoms on water ice.
Astrochemical models widely assume that H atoms are mobile on the surface of
dust grains even if controversy still exists. We present here direct
experimental evidence of the mobility of H atoms on porous water ice surfaces
at 10 K. Methods. In a UHV chamber, O2 is deposited on a porous amorphous water
ice substrate. Then D atoms are deposited onto the surface held at 10 K.
Temperature-Programmed Desorption (TPD) is used and desorptions of O2 and D2
are simultaneously monitored. Results. We find that the amount of O2 that
desorb during the TPD diminishes if we increase the deposition time of D atoms.
O2 is thus destroyed by D atoms even though these molecules have previously
diffused inside the pores of thick water ice. Our results can be easily
interpreted if D is mobile at 10 K on the water ice surface. A simple rate
equation model fits our experimental data and best fit curves were obtained for
a D atoms diffusion barrier of 22(+-)2 meV. Therefore hydrogenation can take
place efficiently on interstellar dust grains. These experimental results are
in line with most calculations and validate the hypothesis used in several
models.Comment: 4 pages (Submitted to A&A
X-ray photodesorption of complex organic molecules in protoplanetary disks -- I. Acetonitrile CH3CN
X-rays emitted from pre-main-sequence stars at the center of protoplanetary
disks can induce nonthermal desorption from interstellar ices populating the
cold regions. This X-ray photodesorption needs to be quantified for complex
organic molecules (COMs), including acetonitrile CH3CN, which has been detected
in several disks. We experimentally estimate the X-ray photodesorption yields
of neutral species from pure CH3CN ices and from interstellar ice analogs for
which CH3CN is mixed either in a CO- or H2O-dominated ice. The ices were
irradiated at 15 K by soft X-rays (400-600 eV) from synchrotron light (SOLEIL
synchrotron). X-ray photodesorption was probed in the gas phase via quadrupole
mass spectrometry. X-ray photodesorption yields were derived from the mass
signals and were extrapolated to higher X-ray energies for astrochemical
models. X-ray photodesorption of the intact CH3CN is detected from pure CH3CN
ices and from mixed 13CO:CH3CN ices, with a yield of about 5x10^(-4)
molecules/photon at 560 eV. When mixed in H2O-dominated ices, X-ray
photodesorption of the intact CH3CN at 560 eV is below its detection limit,
which is 10^(-4) molecules/photon. Yields associated with the desorption of
HCN, CH4 , and CH3 are also provided. The derived astrophysical yields
significantly depend on the local conditions expected in protoplanetary disks.
They vary from 10^(-4) to 10(-6) molecules/photon for the X-ray photodesorption
of intact CH3CN from CO-dominated ices. Only upper limits varying from
5x10^(-5) to 5x10^(-7) molecules/photon could be derived for the X-ray
photodesorption of intact CH3CN from H2O-dominated ices. X-ray photodesorption
of intact CH3CN from interstellar ices might in part explain the abundances of
CH3CN observed in protoplanetary disks. The desorption efficiency is expected
to vary with the local physical conditions, hence with the disk region
Pre-clinical studies of bone regeneration with human bone marrow stromal cells and biphasic calcium phosphate
Introduction
Repair of large bone defects remains a significant clinical challenge. Bone marrow stromal cells (BMSCs), a subset of which is known as bone marrow-derived mesenchymal stem cells, show therapeutic potential for bone regeneration. However, their isolation, expansion and implantation will need to be conducted under good manufacturing practices (GMP) at separate locations. An investigation which mimics this clinical scenario where large bone defects shall be regenerated is required before clinical trials can be initiated.
Methods
Seven batches of 100 million human ex-vivo expanded BMSCs from five donors were transported fresh in syringes from a GMP facility in Germany to France. BMSCs were mixed with biphasic calcium phosphate (BCP) biomaterial prior to subcutaneous implantation in nude mice. The capacity of BMSCs in unison with BCP to regenerate critical sized cranial bone defects was also evaluated. BMSCs expressing luciferase were used to assess the viability and bio-distribution of implanted cells. In situ hybridization, using the human-specific repetitive Alu sequence, was performed for the identification of human cells in explants.
Results
Eight weeks after implantation of BMSCs, mineralized bone containing mature bone marrow territories was formed in ectopic sites and in calvaria defects. Significant loss of cell viability was observed by bioluminescence imaging and only 1.5 percent of the initial number of transplanted cells remained after 37 days. After eight weeks, while explants were comprised primarily of host cells, there were also human cells attached along the periphery of BCP and embedded in osteocyte lacunae dispersed throughout the newly formed bone matrix.
Conclusions
This study demonstrates the safety and efficacy of BMSC/BCP combinations and provides crucial information for the implementation of BMSC therapy for bone regeneration
The functional trait spectrum of European temperate grasslands
Questions: What is the functional trait variation of European temperate grasslands and how does this reflect global patterns of plant form and function? Do habitat specialists show trait differentiation across habitat types?. Location: Europe. Methods: We compiled 18 regeneration and non-regeneration traits for a continental species pool consisting of 645 species frequent in five grassland types. These grassland types are widely distributed in Europe but differentiated by altitude, soil bedrock and traditional long-term management and disturbance regimes. We evaluated the multivariate trait space of this entire species pool and compared multi-trait variation and mean trait values of habitat specialists grouped by grassland type. Results: The first dimension of the trait space accounted for 23% of variation and reflected a gradient between fast-growing and slow-growing plants. Plant height and SLA contributed to both the first and second ordination axes. Regeneration traits mainly contributed to the second and following dimensions to explain 56% of variation across the first five axes. Habitat specialists showed functional differences between grassland types mainly through non-regeneration traits. Conclusions: The trait spectrum of plants dominating European temperate grasslands is primarily explained by growth strategies which are analogous to the trait variation observed at the global scale, and secondly by regeneration strategies. Functional differentiation of habitat specialists across grassland types is mainly related to environmental filtering linked with altitude and disturbance. This filtering pattern is mainly observed in non-regeneration traits, while most regeneration traits demonstrate multiple strategies within the same habitat type.EL, BJA, MTI, AM, PI and CB acknowledge the research leading to these results has received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007–2013 under REA grant agreement no. 607785, as a part of the NAtive Seed Science TEchnology and Conservation (NASSTEC) Initial Training Network (ITN). BJA was further funded by the Marie Curie Clarín‐COFUND program of the Principality of Asturias and the European Union (ACB17‐26). BJA and HB acknowledge support from the German Centre for Integrative Biodiversity Research (iDiv) Halle–Jena–Leipzig funded by the German Research Foundation (DFTG FZT 118) through the sPlot research platform. PI acknowledges support from the Rural & Environment Science & Analytical Services Division of the Scottish Government. KÖ thanks RO1567‐IBB03/2018 for financial support
Experimental evidence for water formation on interstellar dust grains by hydrogen and oxygen atoms
Context. The synthesis of water is one necessary step in the origin and
development of life. It is believed that pristine water is formed and grows on
the surface of icy dust grains in dark interstellar clouds. Until now, there
has been no experimental evidence whether this scenario is feasible or not on
an astrophysically relevant template and by hydrogen and oxygen atom reactions.
Aims. We present here the first experimental evidence of water synthesis by
such a process on a realistic grain surface analogue in dense clouds, i.e.,
amorphous water ice.
Methods. Atomic beams of oxygen and deuterium are aimed at a porous water ice
substrate (H2O) held at 10 K. Products are analyzed by the
temperature-programmed desorption technique.
Results. We observe production of HDO and D2O, indicating that water is
formed under conditions of the dense interstellar medium from hydrogen and
oxygen atoms. This experiment opens up the field of a little explored complex
chemistry that could occur on dust grains, believed to be the site where key
processes lead to the molecular diversity and complexity observed in the
Universe.Comment: 8 pages, 2 figures, 1 table. Submitted to A&
Grain Surface Models and Data for Astrochemistry
AbstractThe cross-disciplinary field of astrochemistry exists to understand the formation, destruction, and survival of molecules in astrophysical environments. Molecules in space are synthesized via a large variety of gas-phase reactions, and reactions on dust-grain surfaces, where the surface acts as a catalyst. A broad consensus has been reached in the astrochemistry community on how to suitably treat gas-phase processes in models, and also on how to present the necessary reaction data in databases; however, no such consensus has yet been reached for grain-surface processes. A team of ∼25 experts covering observational, laboratory and theoretical (astro)chemistry met in summer of 2014 at the Lorentz Center in Leiden with the aim to provide solutions for this problem and to review the current state-of-the-art of grain surface models, both in terms of technical implementation into models as well as the most up-to-date information available from experiments and chemical computations. This review builds on the results of this workshop and gives an outlook for future directions
TRY plant trait database - enhanced coverage and open access
Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives
Severe Compromise of Preosteoblasts in a Surgical Mouse Model of Bisphosphonate-Associated Osteonecrosis of the Jaw.
Objectives: The effect of amino-bisphosphonates on osteoblastic lineage and its potential
contribution to the pathogenesis of bisphosphonate-associated osteonecrosis of the jaw
(BONJ) remain controversial. We assessed the effects of zoledronic acid (ZOL) on bone and
vascular cells of the alveolar socket using a mouse model of BONJ.
Material and Methods: Thirty-two mice were treated twice a week with either 100 μg/kg of
ZOL or saline for 12 weeks. The first left maxillary molar was extracted at the third week.
Alveolar sockets were assessed at both 3 weeks (intermediate) and 9 weeks (long-term) after
molar extraction by semi-quantitative histomorphometry for empty lacunae, preosteoblasts
(Osterix), osteoclasts (TRAP), and pericyte-like cells (CD146). Also, the bone
microarchitecture was assessed by micro-CT.
Results: Osteonecrotic-like lesions were observed in 21% of mice. Moreover, a decreased
number of preosteoblasts contrasted with the increased number of osteoclasts at both time
points. In addition, osteoclasts display multinucleation and detachment from the endosteal
surface. Furthermore, the number of pericyte-like cells increased at the intermediate time
point. The alveolar bone mass increased exclusively with long-term ZOL treatment.
Conclusion: The severe imbalance between bone-forming cells and bone-resorbing cells
showed in this study could contribute to the pathogenesis of BONJ
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