496 research outputs found
The implication of personal selling strategies in motivation, approaches and good grooming
This study focuses on the use of strategies in motivation, approaches and good grooming in the personal selling of cosmetic products.The descriptive-purposive method of research was used; One Way Analysis of Variances statistic interpreted the correlates of personal selling strategies on motivation, approaches and good grooming.It showed that the use of strategies in motivation, approaches, and good grooming are significantly correlated.The questionnaire was validated to 12 respondents from consumers and sellers of cosmetics and floated to 140 respondents of four selected retail outlets that sell facial cosmetic products in Santiago City, Philippines.It is expected that the strategic personal selling model plan for effective salesmanship will be proposed
HFE gene mutations and iron status in healthy volunteers from northwestern Argentina
IntroducciĂłn: La proteĂna HFE (Human hemochromatosis protein) es codificada por el gen HFE y participa en la regulaciĂłn del metabolismo del hierro. Tres variantes alĂ©licas del gen HFE se han correlacionado con la hemocromatosis hereditaria: C282Y, H63D y S65C. Objetivos: Establecer la prevalencia de las mutaciones mĂĄs frecuentes en el gen HFE en una poblaciĂłn de individuos aparentemente sanos y estudiar su relaciĂłn con el metabolismo del hierro. Materiales y MĂ©todos: Sesenta y nueve individuos (48 mujeres y 21 hombres) fueron analizados durante el perĂodo de septiembre de 2015 a agosto de 2018 en TucumĂĄn, Argentina. Se determinĂł hemograma, hierro, transferrina, saturaciĂłn y ferritina. Las mutaciones en el gen HFE se efectuaron mediante PCR en tiempo real. Resultados: La prevalencia fue del 28 % (19/69; IC95% = 18-39%). El 9 % (6/69) presentĂł mutaciĂłn heterocigota C282Y y 19 % (13/69; 11 heterocigotos y 2 homocigotos), en codĂłn 63 (H63D). No se detectĂł la mutaciĂłn S65C. En los individuos que no portaban mutaciĂłn, los parĂĄmetros de la serie roja y el anĂĄlisis de hierro fueron mĂĄs altos en los hombres que en las mujeres (p 0,05). Conclusiones: Este es el primer estudio que muestra la frecuencia de las tres variantes del gen de la hemocromatosis en el noroeste argentino. La mutaciĂłn mĂĄs prevalente fue la H63D. La presencia de la mutaciĂłn en el gen HFE no resultĂł en una sobrecarga bioquĂmica significativa de hierro.Introduction: The HFE protein, encoded by the HFE gene, participates in iron homeostasis regulation. Three allelic variants of the HFE gene have been correlated with hereditary hemochromatosis: C282Y, H63D and S65C. Objectives: The purpose of this study was to establish the prevalence of the most frequent mutations in the HFE gene in an apparently healthy population of northwestern Argentina, and to study its relationship with iron metabolism. Materials and Methods: Sixty-nine individuals (48 women and 21 men) were analyzed during the period September 2015-August 2018 in TucumĂĄn, Argentina. Total blood count and iron parameters were measured. Mutations in the HFE gene were determined by real-time PCR. Results: The prevalence was 28% (19/69; CI95%= 18-39%). Nine percent (9%, 6/69) had a heterozygous C282Y mutation, and 19% showed an H63D mutation (13/69; 11 heterozygous and 2 homozygous). The S65C mutation was not detected. Red blood cell parameters and iron analysis were higher in wild-type men than in wild-type women (p0.05). Conclusions: This is the first study to show the frequency of the three hemochromatosis gene variants in the northwest region of Argentina, and to determine that the H63D mutation was the most prevalent. This HFE mutation did not result in significant biochemical iron overload.Fil: Monaco, Maria Eugenia. Universidad Nacional de TucumĂĄn. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BiologĂa; ArgentinaFil: Alvarez Asensio, Natalia SofĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn; Argentina. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Haro, Ana Cecilia. Universidad Nacional de TucumĂĄn. Instituto de BiotecnologĂa FarmacĂ©utica y Alimentaria. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto de BiotecnologĂa FarmacĂ©utica y Alimentaria; Argentina. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Teran, Magdalena MarĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn; Argentina. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Ledesma Achem, Myriam Emilia. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Isse, Blanca Alicia de Los Angeles G.. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Lazarte, Sandra Stella. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; Argentin
HFE gene mutations and iron status in healthy volunteers from northwestern Argentina
IntroducciĂłn: La proteĂna HFE (Human hemochromatosis protein) es codificada por el gen HFE y participa en la regulaciĂłn del metabolismo del hierro. Tres variantes alĂ©licas del gen HFE se han correlacionado con la hemocromatosis hereditaria: C282Y, H63D y S65C. Objetivos: Establecer la prevalencia de las mutaciones mĂĄs frecuentes en el gen HFE en una poblaciĂłn de individuos aparentemente sanos y estudiar su relaciĂłn con el metabolismo del hierro. Materiales y MĂ©todos: Sesenta y nueve individuos (48 mujeres y 21 hombres) fueron analizados durante el perĂodo de septiembre de 2015 a agosto de 2018 en TucumĂĄn, Argentina. Se determinĂł hemograma, hierro, transferrina, saturaciĂłn y ferritina. Las mutaciones en el gen HFE se efectuaron mediante PCR en tiempo real. Resultados: La prevalencia fue del 28 % (19/69; IC95% = 18-39%). El 9 % (6/69) presentĂł mutaciĂłn heterocigota C282Y y 19 % (13/69; 11 heterocigotos y 2 homocigotos), en codĂłn 63 (H63D). No se detectĂł la mutaciĂłn S65C. En los individuos que no portaban mutaciĂłn, los parĂĄmetros de la serie roja y el anĂĄlisis de hierro fueron mĂĄs altos en los hombres que en las mujeres (p 0,05). Conclusiones: Este es el primer estudio que muestra la frecuencia de las tres variantes del gen de la hemocromatosis en el noroeste argentino. La mutaciĂłn mĂĄs prevalente fue la H63D. La presencia de la mutaciĂłn en el gen HFE no resultĂł en una sobrecarga bioquĂmica significativa de hierro.Introduction: The HFE protein, encoded by the HFE gene, participates in iron homeostasis regulation. Three allelic variants of the HFE gene have been correlated with hereditary hemochromatosis: C282Y, H63D and S65C. Objectives: The purpose of this study was to establish the prevalence of the most frequent mutations in the HFE gene in an apparently healthy population of northwestern Argentina, and to study its relationship with iron metabolism. Materials and Methods: Sixty-nine individuals (48 women and 21 men) were analyzed during the period September 2015-August 2018 in TucumĂĄn, Argentina. Total blood count and iron parameters were measured. Mutations in the HFE gene were determined by real-time PCR. Results: The prevalence was 28% (19/69; CI95%= 18-39%). Nine percent (9%, 6/69) had a heterozygous C282Y mutation, and 19% showed an H63D mutation (13/69; 11 heterozygous and 2 homozygous). The S65C mutation was not detected. Red blood cell parameters and iron analysis were higher in wild-type men than in wild-type women (p0.05). Conclusions: This is the first study to show the frequency of the three hemochromatosis gene variants in the northwest region of Argentina, and to determine that the H63D mutation was the most prevalent. This HFE mutation did not result in significant biochemical iron overload.Fil: Monaco, Maria Eugenia. Universidad Nacional de TucumĂĄn. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BiologĂa; ArgentinaFil: Alvarez Asensio, Natalia SofĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn; Argentina. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Haro, Ana Cecilia. Universidad Nacional de TucumĂĄn. Instituto de BiotecnologĂa FarmacĂ©utica y Alimentaria. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto de BiotecnologĂa FarmacĂ©utica y Alimentaria; Argentina. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Teran, Magdalena MarĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn; Argentina. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Ledesma Achem, Myriam Emilia. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Isse, Blanca Alicia de Los Angeles G.. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; ArgentinaFil: Lazarte, Sandra Stella. Universidad Nacional de Tucuman. Facultad de BioquĂmica, QuĂmica y Farmacia. Instituto de BioquĂmica Clinica Aplicada. CĂĄtedra de BioquĂmica Clinica I; Argentin
Combined use of gliadins and SSRs to analyse the genetic variability of the Spanish collection of cultivated diploid wheat (Triticum monococcum L. ssp. monococcum)
This work studied the combined use of gliadins and SSRs to analyse inter- and intra-accession variability of the Spanish collection of cultivated einkorn (Triticum monococcum L. ssp. monococcum) maintained at the CRF-INIA. In general, gliadin loci presented higher discrimination power than SSRs, reflecting the high variability of the gliadins. The loci on chromosome 6A were the most polymorphic with similar PIC values for both marker systems, showing that these markers are very useful for genetic variability studies in wheat. The gliadin results indicated that the Spanish einkorn collection possessed high genetic diversity, being the differentiation large between varieties and small within them. Some associations between gliadin alleles and geographical and agro-morphological data were found. Agro-morphological relations were also observed in the clusters of the SSRs dendrogram. A high concordance was found between gliadins and SSRs for genotype identification. In addition, both systems provide complementary information to resolve the different cases of intra-accession variability not detected at the agro-morphological level, and to identify separately all the genotypes analysed. The combined use of both genetic markers is an excellent tool for genetic resource evaluation in addition to agro-morphological evaluation
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Haplotype analysis of the internationally distributed BRCA1 c.3331_3334delCAAG founder mutation reveals a common ancestral origin in Iberia
Background: TheBRCA1c.3331_3334delCAAG founder mutation has been reported in hereditary breast and ovarian cancer families from multiple Hispanic groups. We aimed to evaluateBRCA1c.3331_3334delCAAG haplotype diversity in cases of European, African, and Latin American ancestry. Methods: BC mutation carrier cases from Colombia (n = 32), Spain (n = 13), Portugal (n = 2), Chile (n = 10), Africa (n = 1), and Brazil (n = 2) were genotyped with the genome-wide single nucleotide polymorphism (SNP) arrays to evaluate haplotype diversity aroundBRCA1c.3331_3334delCAAG. Additional Portuguese (n = 13) and Brazilian (n = 18) BC mutation carriers were genotyped for 15 informative SNPs surroundingBRCA1. Data were phased using SHAPEIT2, and identical by descent regions were determined using BEAGLE and GERMLINE. DMLE+ was used to date the mutation in Colombia and Iberia. Results: The haplotype reconstruction revealed a shared 264.4-kb region among carriers from all six countries. The estimated mutation age was similar to 100 generations in Iberia and that it was introduced to South America early during the European colonization period. Conclusions: Our results suggest that this mutation originated in Iberia and later introduced to Colombia and South America at the time of Spanish colonization during the early 1500s. We also found that the Colombian mutation carriers had higher European ancestry, at the BRCA1 gene harboring chromosome 17, than controls, which further supported the European origin of the mutation. Understanding founder mutations in diverse populations has implications in implementing cost-effective, ancestry-informed screening
Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
Sterols are crucial components of biological membranes, which are synthetized in the ER and accumulate in the plasma membrane (PM). Here, by applying a genetically encoded sterol biosensor (D4H), we visualize a sterol flow between PM and endosomes in the fission yeast Schizosaccharomyces pombe. Using time-lapse and correlative light-electron microscopy, we found that inhibition of Arp2/3-dependent F-actin assembly promotes the reversible relocalization of D4H from the PM to internal sterol-rich compartments (STRIC) labeled by synaptobrevin Syb1. Retrograde sterol internalization to STRIC is independent of endocytosis or an intact Golgi, but depends on Ltc1, a LAM/StARkin-family protein localized to ER-PM contact sites. The PM in ltc1Î cells over-accumulates sterols and upon Arp2/3 inhibition forms extended ER-interacting invaginations, indicating that sterol transfer contributes to PM size homeostasis. Anterograde sterol movement from STRIC is independent of canonical vesicular trafficking but requires Arp2/3, suggesting a novel role for this complex. Thus, transfer routes orthogonal to vesicular trafficking govern the flow of sterols in the cell
COVID-19 vaccination in patients receiving allergen immunotherapy (AIT) or biologicals:EAACI recommendations
Immune modulation is a key therapeutic approach for allergic diseases, asthma and autoimmunity. It can be achieved in an antigen-specific manner via allergen immunotherapy (AIT) or in an endotype-driven approach using biologicals that target the major pathways of the type 2 (T2) immune response: immunoglobulin (Ig)E, interleukin (IL)-5 and IL-4/IL-13 or non-type 2 response: anti-cytokine antibodies and B-cell depletion via anti-CD20. Coronavirus disease 2019 (COVID-19) vaccination provides an excellent opportunity to tackle the global pandemics and is currently being applied in an accelerated rhythm worldwide. The vaccine exerts its effects through immune modulation, induces and amplifies the response against the severe acute respiratory syndrome coronavirus (SARS-CoV-2). Thus, as there may be a discernible interference between these treatment modalities, recommendations on how they should be applied in sequence are expected. The European Academy of Allergy and Clinical Immunology (EAACI) assembled an expert panel under its Research and Outreach Committee (ROC). This expert panel evaluated the evidence and have formulated recommendations on the administration of COVID-19 vaccine in patients with allergic diseases and asthma receiving AIT or biologicals. The panel also formulated recommendations for COVID-19 vaccine in association with biologicals targeting the type 1 or type 3 immune response. In formulating recommendations, the panel evaluated the mechanisms of COVID-19 infection, of COVID-19 vaccine, of AIT and of biologicals and considered the data published for other anti-infectious vaccines administered concurrently with AIT or biologicals
The large trans-Neptunian object 2002 TC302 from combined stellar occultation, photometry, and astrometry data
Context. Deriving physical properties of trans-Neptunian objects is important for the understanding of our Solar System. This requires observational efforts and the development of techniques suitable for these studies. Aims. Our aim is to characterize the large trans-Neptunian object (TNO) 2002 TC302. Methods. Stellar occultations offer unique opportunities to determine key physical properties of TNOs. On 28 January 2018, 2002 TC302 occulted a mv ~ 15.3 star with designation 593-005847 in the UCAC4 stellar catalog, corresponding to Gaia source 130957813463146112. Twelve positive occultation chords were obtained from Italy, France, Slovenia, and Switzerland. Also, four negative detections were obtained near the north and south limbs. This represents the best observed stellar occultation by a TNO other than Pluto in terms of the number of chords published thus far. From the 12 chords, an accurate elliptical fit to the instantaneous projection of the body can be obtained that is compatible with the near misses. Results. The resulting ellipse has major and minor axes of 543 ± 18 km and 460 ± 11 km, respectively, with a position angle of 3 ± 1 degrees for the minor axis. This information, combined with rotational light curves obtained with the 1.5 m telescope at Sierra Nevada Observatory and the 1.23 m telescope at Calar Alto observatory, allows us to derive possible three-dimensional shapes and density estimations for the body based on hydrostatic equilibrium assumptions. The effective diameter in equivalent area is around 84 km smaller than the radiometrically derived diameter using thermal data from Herschel and Spitzer Space Telescopes. This might indicate the existence of an unresolved satellite of up to ~300 km in diameter, which is required to account for all the thermal flux, although the occultation and thermal diameters are compatible within their error bars given the considerable uncertainty of the thermal results. The existence of a potential satellite also appears to be consistent with other ground-based data presented here. From the effective occultation diameter combined with absolute magnitude measurements we derive a geometric albedo of 0.147 ± 0.005, which would be somewhat smaller if 2002 TC302 has a satellite. The best occultation light curves do not show any signs of ring features or any signatures of a global atmosphere.Funding from Spanish projects AYA2014-56637-C2-1-P, AYA2017-89637-R, from FEDER, and Proyecto de Excelencia de la Junta de AndalucĂa 2012-FQM1776 is acknowledged. We would like to acknowledge financial support by the Spanish grant AYA-RTI2018-098657-JI00 âLEO-SBNAFâ (MCIU/AEI/FEDER, UE) and the financial support from the State Agency for Research of the Spanish MCIU through the âCenter of Excellence Severo Ochoaâ award for the Instituto de AstrofĂsica de AndalucĂa (SEV- 2017-0709). Part of the research received funding from the European Unionâs Horizon 2020 Research and Innovation Programme, under grant agreement no. 687378 and from the ERC programme under Grant Agreement no. 669416 Lucky Star. The following authors acknowledge the respective CNPq grants: FB-R 309578/2017-5; RV-M 304544/2017-5, 401903/2016-8; J.I.B.C. 308150/2016-3; MA 427700/2018-3, 310683/2017-3, 473002/2013-2. This study was financed in part by the Coordenação de Aperfeiaçoamento de Pessoal de NĂvel Superior - Brasil (CAPES) - Finance Code 001 and the National Institute of Science and Technology of the e-Universe project (INCT do e-Universo, CNPq grant 465376/2014-2). GBR acknowledges CAPES-FAPERJ/PAPDRJ grant E26/203.173/2016, MA FAPERJ grant E-26/111.488/2013 and ARGJr FAPESP grant 2018/11239-8. E.F.-V. acknowledges support from the 2017 Preeminent Postdoctoral Program (P3) at UCF. C.K., R.S., A.F-T., and G.M. have been supported by the K-125015 and GINOP-2.3.2-15-2016-00003 grants of the Hungarian National Research, Development and Innovation Office (NKFIH), Hungary. G.M. was also supported by the Hungarian National Research, Development and Innovation Office (NKFIH) grant PD-128 360. R.K. and T.P. were supported by the VEGA 2/0031/18 grant
Influence of the Temperature and the Genotype of the HSP90AA1 Gene over Sperm Chromatin Stability in Manchega Rams
The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37°C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity index (THI) from day 60 prior collection to the date of semen collection on DFI were examined. To better understand the causes determining the sensitivity of spermatozoa to heat, this study was conducted in 60 males with alternative genotypes for the SNP G/Câ660 of the HSP90AA1 promoter, which encode for the Hsp90α protein. The Hsp90α protein predominates in the brain and testis, and its role in spermatogenesis has been described in several species. Ridge regression analyses showed that days 29 to 35 and 7 to 14 before sperm collection (bsc) were the most critical regarding the effect of heat stress over DFI values. Mixed model analyses revealed that DFI increases over a threshold of 30°C for maximum temperature and 22 for THI at days 29 to 35 and 7 to 14 bsc only in animals carrying the GGâ660 genotype. The period 29â35 bsc coincide with the meiosis I process for which the effect of the Hsp90α has been described in mice. The period 7â14 bsc may correspond with later stages of the meiosis II and early stages of epididymal maturation in which the replacement of histones by protamines occurs. Because of GGâ660 genotype has been associated to lower levels of HSP90AA1 expression, suboptimal amounts of HSP90AA1 mRNA in GGâ660 animals under heat stress conditions make spermatozoa DNA more susceptible to be fragmented. Thus, selecting against the GGâ660 genotype could decrease the DNA fragmentation and spermatozoa thermal susceptibility in the heat season, and its putative subsequent fertility gainsPublishe
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