Relación entre el clima social familiar y habilidades sociales en adolescentes trabajadores que asisten a dos escuelas públicas de Lima Metropolitana

El objetivo del presente trabajo fue conocer la relación entre el clima social familiar y las habilidades sociales en adolescentes trabajadores que asisten a dos escuelas públicas de Lima metropolitana. La muestra la conformaron 123 adolescentes trabajadores de 12 a 14 años de edad. Se utilizaron la Escala del Clima Social Familiar (FES) y la Escala de Habilidades Sociales (EHS). Los resultados indican correlaciones moderadas entre el clima social familiar y las habilidades sociales. Se halló también que dicha relación es más fuerte en las adolescentes que en los adolescentes. También se encontró que más de la mitad de los participantes presentan puntuaciones entre medias y altas en todas las variables

Factores Socioambientales en la neumonía de niños menores de 1 año hospitalizados en el servicio de emergencia pediátrica del Hospital Nacional Cayetano Heredia, Lima – 2019

El OBJETIVO del presente estudio fue determinar los factores sociambientales que se asocian a la Neumonía de niños menores de 1 año hospitalizados en el servicio de Emergencia Pediátrica del Hospital Nacional Cayetano Heredia en mayo 2019. El DISEÑO METODOLÓGICO fue aplicada, descriptivo correlacional, prospectivo y de corte transversal; la muestra fue de 66 madres, donde se les explico sobre el cuestionario que se les aplicaría para conocer acerca de los factores sociales, ambientales y el número de ingresos por neumonías de sus hijos, para el análisis estadístico se utilizó la prueba de Chi-cuadrado en el programa SPSS. Los RESULTADOS fueron: el nivel de instrucción de la madre como el empleo fuera de casa muestran asociación con el número de ingresos por neumonía en niños menores de 1 año (p<0.05), la humedad en la vivienda, partículas de polvo en el aire y el adecuado manejo de residuos sólidos muestran asociación con el número de ingresos por neumonía en niños menores de 1 año (p<0.05). Las CONCLUSIONES fueron: existe asociación significativa entre los factores sociambientales y la neumonía en niños menores de 1 año; existe asociación significativa con un valor de p< 0.05 entre los factores sociales y la Neumonía en niños menores de 1 año y existe asociación significativa con un valor de p< 0.05 entre los factores ambientales y la Neumonía en niños menores de 1 año hospitalizados en el servicio de Emergencia Pediátrica del Hospital Nacional Cayetano Heredia.Tesis de segunda especialida

Micropropagación de especies endémicas del Bosque La Primavera y caracterización de suelos

Este trabajo presenta la elaboración de técnicas de micropropagación de especies vegetales de interés ecológico, con el fin de aumentar la reserva de plantas del ANP Bosque La Primavera, y como consecuencia, preservar el ecosistema y contrarrestar los daños provocados por fenómenos naturales-antropogénicos. Se analizó el suelo del ANP Bosque La Primavera en distintos puntos para obtener información sobre sus características.ITESO, A.C

Plasma sTNFR1 and IL8 for prognostic enrichment in sepsis trials: a prospective cohort study.

BackgroundEnrichment strategies improve therapeutic targeting and trial efficiency, but enrichment factors for sepsis trials are lacking. We determined whether concentrations of soluble tumor necrosis factor receptor-1 (sTNFR1), interleukin-8 (IL8), and angiopoietin-2 (Ang2) could identify sepsis patients at higher mortality risk and serve as prognostic enrichment factors.MethodsIn a multicenter prospective cohort study of 400 critically ill septic patients, we derived and validated thresholds for each marker and expressed prognostic enrichment using risk differences (RD) of 30-day mortality as predictive values. We then used decision curve analysis to simulate the prognostic enrichment of each marker and compare different prognostic enrichment strategies.Measurements and main resultsAn admission sTNFR1 concentration &gt; 8861 pg/ml identified patients with increased mortality in both the derivation (RD 21.6%) and validation (RD 17.8%) populations. Among immunocompetent patients, an IL8 concentration &gt; 94 pg/ml identified patients with increased mortality in both the derivation (RD 17.7%) and validation (RD 27.0%) populations. An Ang2 level &gt; 9761 pg/ml identified patients at 21.3% and 12.3% increased risk of mortality in the derivation and validation populations, respectively. Using sTNFR1 or IL8 to select high-risk patients improved clinical trial power and efficiency compared to selecting patients with septic shock. Ang2 did not outperform septic shock as an enrichment factor.ConclusionsThresholds for sTNFR1 and IL8 consistently identified sepsis patients with higher mortality risk and may have utility for prognostic enrichment in sepsis trials

An Epilepsy-Causing Mutation Leads to Co-Translational Misfolding of the Kv7.2 Channel

BACKGROUND: The amino acid sequence of proteins generally carries all the necessary information for acquisition of native conformations, but the vectorial nature of translation can additionally determine the folding outcome. Such consideration is particularly relevant in human diseases associated to inherited mutations leading to structural instability, aggregation, and degradation. Mutations in the KCNQ2 gene associated with human epilepsy have been suggested to cause misfolding of the encoded Kv7.2 channel. Although the effect on folding of mutations in some domains has been studied, little is known of the way pathogenic variants located in the calcium responsive domain (CRD) affect folding. Here, we explore how a Kv7.2 mutation (W344R) located in helix A of the CRD and associated with hereditary epilepsy interferes with channel function. RESULTS: We report that the epilepsy W344R mutation within the IQ motif of CRD decreases channel function, but contrary to other mutations at this site, it does not impair the interaction with Calmodulin (CaM) in vitro, as monitored by multiple in vitro binding assays. We find negligible impact of the mutation on the structure of the complex by molecular dynamic computations. In silico studies revealed two orientations of the side chain, which are differentially populated by WT and W344R variants. Binding to CaM is impaired when the mutated protein is produced in cellulo but not in vitro, suggesting that this mutation impedes proper folding during translation within the cell by forcing the nascent chain to follow a folding route that leads to a non-native configuration, and thereby generating non-functional ion channels that fail to traffic to proper neuronal compartments. CONCLUSIONS: Our data suggest that the key pathogenic mechanism of Kv7.2 W344R mutation involves the failure to adopt a configuration that can be recognized by CaM in vivo but not in vitroThe Government of the Autonomous Community of the Basque Country (IT1165-19 and KK-2020/00110) and the Spanish Ministry of Science and Innovation (RTI2018-097839-B-100 to A.V. and FIS2016-76617-P to A.B.) and FEDER funds and the US National Institute of Neurological Disorders (NINDS) and Stroke Research Project Grant (R01NS083402 to H.J.C.) provided financial support for this work. E.N. and A.M-M. are supported by predoctoral contracts from the Basque Government administered by University of the Basque Country. C.M. was supported by the Basque Government through a Basque Excellence Research Centre (BERC) grant administered by Fundación Biofisika Bizkaia (FBB). J.U. was partially supported by BERC funds. O.R.B. was supported by the Basque Government through a BERC grant administered by Donostia International Physics Center. J.Z. and H.J.C. was supported by the NINDS Research Project Grant #R01NS083402 (PI: H.J.C.)

Mutation spectrum of NOD2 reveals recessive inheritance as a main driver of Early Onset Crohn’s Disease

Inflammatory bowel disease (IBD), clinically defined as Crohn’s disease (CD), ulcerative colitis (UC), or IBD-unclassified, results in chronic inflammation of the gastrointestinal tract in genetically susceptible hosts. Pediatric onset IBD represents ≥ 25% of all IBD diagnoses and often presents with intestinal stricturing, perianal disease, and failed response to conventional treatments. NOD2 was the first and is the most replicated locus associated with adult IBD, to date. However, its role in pediatric onset IBD is not well understood. We performed whole-exome sequencing on a cohort of 1,183 patients with pediatric onset IBD (ages 0–18.5 years). We identified 92 probands with biallelic rare and low frequency NOD2 variants accounting for approximately 8% of our cohort, suggesting a Mendelian inheritance pattern of disease. Additionally, we investigated the contribution of recessive inheritance of NOD2 alleles in adult IBD patients from a large clinical population cohort. We found that recessive inheritance of NOD2 variants explains ~ 7% of cases in this adult IBD cohort, including ~ 10% of CD cases, confirming the observations from our pediatric IBD cohort. Exploration of EHR data showed that several of these adult IBD patients obtained their initial IBD diagnosis before 18 years of age, consistent with early onset disease. While it has been previously reported that carriers of more than one NOD2 risk alleles have increased susceptibility to Crohn’s Disease (CD), our data formally demonstrate that recessive inheritance of NOD2 alleles is a mechanistic driver of early onset IBD, specifically CD, likely due to loss of NOD2 protein function. Collectively, our findings show that recessive inheritance of rare and low frequency deleterious NOD2 variants account for 7–10% of CD cases and implicate NOD2 as a Mendelian disease gene for early onset Crohn’s Disease

A combined microbial and biogeochemical dataset from high-latitude ecosystems with respect to methane cycle.

High latitudes are experiencing intense ecosystem changes with climate warming. The underlying methane (CH4) cycling dynamics remain unresolved, despite its crucial climatic feedback. Atmospheric CH4 emissions are heterogeneous, resulting from local geochemical drivers, global climatic factors, and microbial production/consumption balance. Holistic studies are mandatory to capture CH4 cycling complexity. Here, we report a large set of integrated microbial and biogeochemical data from 387 samples, using a concerted sampling strategy and experimental protocols. The study followed international standards to ensure inter-comparisons of data amongst three high-latitude regions: Alaska, Siberia, and Patagonia. The dataset encompasses diferent representative environmental features (e.g. lake, wetland, tundra, forest soil) of these high-latitude sites and their respective heterogeneity (e.g. characteristic microtopographic patterns). The data included physicochemical parameters, greenhouse gas concentrations and emissions, organic matter characterization, trace elements and nutrients, isotopes, microbial quantifcation and composition. This dataset addresses the need for a robust physicochemical framework to conduct and contextualize future research on the interactions between climate change, biogeochemical cycles and microbial communities at highlatitudes

Vinculación entre extensión, investigación y docencia a partir del trabajo en red en promoción de la salud

Networking is an articulation and exchange strategy between institutions that decide to bond and associate efforts, experiences and knowledge to reach common objectives. The participation of teachers of the School of Veterinary Sciences of the National University of La Pampa in networks with representatives of the health and education sectors, municipal organisms and NGOs, among others, allowed to generate different extension proposals based on health promotion. Dialogue, communication and construction spaces were created between the university and the community. The objective of this article is to describe linkage activities between the School above mentioned and the community through networking in health promotion. Fifty two meetings in different regions took place and fifty five community activities were organized, which included thirty eight workshops aimed at different actors of the educational community, elders, pregnant women, and public in general and seventeen training activities, seminars, informative talks, active waiting rooms and scientific-technological publication. Different didactic materials were elaborated. Through the socio-environmental problems detected, three research projects were designed. This type of proposals based on networking and health promotion offer an opportunity to higher level institutions to develop their social function. Furthermore, they help teaching, research and extension articulationEl trabajo en red es una estrategia de articulación e intercambio entre instituciones que deciden vincularse y asociar sus esfuerzos, experiencias y conocimientos para el logro de fines comunes. La participación de docentes de la Facultad de Ciencias Veterinarias de la UNLPam en redes conformadas por representantes del sector salud, educación, organismos municipales, ONG, entre otros; permitió generar distintas propuestas de extensión basadas en la promoción de la salud. Se crearon espacios de diálogo, comunicación y construcción de conocimiento entre la universidad y la comunidad. El objetivo de este artículo es describir las actividades de vinculación entre la Facultad de Cs. Veterinarias de la UNLPam y la comunidad a partir del trabajo en red en promoción de la salud. Se participó en 52 reuniones intersectoriales y se realizaron un total de 55 actividades comunitarias, las que incluyeron38 talleres destinados a distintos actores de la comunidad educativa, adultos mayores, embarazadas, público en general y 17 actividades de capacitaciones, seminario, charla informativa, salas de espera activa y divulgación científico-tecnológica. Se elaboraron distintos materiales didácticos A partir de las problemáticas socio-ambientales detectadas, se generaron 3 proyectos de investigación. Este tipo de propuestas basadas en el trabajo en red y en la promoción de la salud ofrecen una oportunidad para que las instituciones de nivel superior desempeñen su función social. Además favorecen la articulación entre la docencia, investigación y extensión DOI:http://dx.doi.org/10.19137/cienvet2013-1511

Riesgo quirúrgico tras resección pulmonar anatómica en cirugía torácica. Modelo predictivo a partir de una base de datos nacional multicéntrica

Introduction: the aim of this study was to develop a surgical risk prediction model in patients undergoing anatomic lung resections from the registry of the Spanish Video-Assisted Thoracic Surgery Group (GEVATS). Methods: data were collected from 3,533 patients undergoing anatomic lung resection for any diagnosis between December 20, 2016 and March 20, 2018. We defined a combined outcome variable: death or Clavien Dindo grade IV complication at 90 days after surgery. Univariate and multivariate analyses were performed by logistic regression. Internal validation of the model was performed using resampling techniques. Results: the incidence of the outcome variable was 4.29% (95% CI 3.6-4.9). The variables remaining in the final logistic model were: age, sex, previous lung cancer resection, dyspnea (mMRC), right pneumonectomy, and ppo DLCO. The performance parameters of the model adjusted by resampling were: C-statistic 0.712 (95% CI 0.648-0.750), Brier score 0.042 and bootstrap shrinkage 0.854. Conclusions: the risk prediction model obtained from the GEVATS database is a simple, valid, and reliable model that is a useful tool for establishing the risk of a patient undergoing anatomic lung resection

Cross-sectional examination of 24-hour movement behaviours among 3-and 4-year-old children in urban and rural settings in low-income, middle-income and high-income countries : the SUNRISE study protocol

Introduction 24-hour movement behaviours (physical activity, sedentary behaviour and sleep) during the early years are associated with health and developmental outcomes, prompting the WHO to develop Global guidelines for physical activity, sedentary behaviour and sleep for children under 5 years of age. Prevalence data on 24-hour movement behaviours is lacking, particularly in low-income and middle-income countries (LMICs). This paper describes the development of the SUNRISE International Study of Movement Behaviours in the Early Years protocol, designed to address this gap. Methods and analysis SUNRISE is the first international cross-sectional study that aims to determine the proportion of 3- and 4-year-old children who meet the WHO Global guidelines. The study will assess if proportions differ by gender, urban/rural location and/or socioeconomic status. Executive function, motor skills and adiposity will be assessed and potential correlates of 24-hour movement behaviours examined. Pilot research from 24 countries (14 LMICs) informed the study design and protocol. Data are collected locally by research staff from partnering institutions who are trained throughout the research process. Piloting of all measures to determine protocol acceptability and feasibility was interrupted by COVID-19 but is nearing completion. At the time of publication 41 countries are participating in the SUNRISE study. Ethics and dissemination The SUNRISE protocol has received ethics approved from the University of Wollongong, Australia, and in each country by the applicable ethics committees. Approval is also sought from any relevant government departments or organisations. The results will inform global efforts to prevent childhood obesity and ensure young children reach their health and developmental potential. Findings on the correlates of movement behaviours can guide future interventions to improve the movement behaviours in culturally specific ways. Study findings will be disseminated via publications, conference presentations and may contribute to the development of local guidelines and public health interventions.Peer reviewe