74 research outputs found
Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M-1s-1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model
Monitoring Redox Dynamics in Living Cells with a Redox-Sensitive Red Fluorescent Protein
Integration of Flex Nozzle System and Electro Hydraulic Actuators to Solid Rocket Motors
Modeling of Microaccelerations Caused by Running of Attitude-Control Engines of Spacecraft with Elastic Structural Elements
PPR3: THE ROLE OF PHARMACOECONOMIC INVESTIGATIONS IN CREATING THE PATIENT'S TREATMENT RECORD
How Does Asymmetry of Solar Panels Influence Constructive Component of Microacceleration Field of Inner Environment of Space Laboratory
Wide-Aperture Liquid Calorimeters for Measuring the Energy of Powerful Microwave Radiation Pulses
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