Effects of blue, orange and white lights on growth, chlorophyll fluorescence, and phycocyanin production of Arthrospira platensis cultures

The aim of this study was to evaluate the effects of different light colors on growth, pigment composition, and photosynthetic performance of Arthrospira platensis. Results showed that under orange light the biomass productivity increased due to the capability of A. platensis to fully absorb this portion of the light spectrum. Under blue light, phycocyanin increased continuously up to 13.2% ± 1.96 of dry weight at day 5, while under orange and white lights the phycocyanin content resulted lower, 7.1 ± 0.39 and 6.7% ± 1.58 of dry weight, respectively. Chlorophyll fluorescence measurements showed the maximum electron transport rate (rETRmax) in cells grown under orange light. The results of this study indicated that the orange light increased both growth and phycocyanin productivities, while blue light increased mostly the phycocyanin content, while biomass productivity was much lower. Further increase of phycocyanin content was observed shifting the light illuminating the cultures from orange to blue, attaining a raise in phycocyanin content from 8.6% to 12.5% of dry weight within 48 h from the start of the illumination with blue light. Within the same period of time no growth was observed indicating that the synthesis of phycocyanin can be decoupled from growth. This study provides useful physiological information regarding the effects of different light spectra on growth, phycocyanin, and photosynthetic performance, as a prerequisite to optimize the production of high value pigments from cultures of A. platensis

Impacto da infecção por nematoides gastrintestinais em cordeiros morada nova.

A raça Morada Nova é considerada resistente à infecção por nematoides gastrintestinais (GINs). Ela também se destaca por sua rusticidade, prolificidade, pele de excelente qualidade, precocidade sexual, excelente habilidade materna e inexistência de sazonalidade reprodutiva

TRY plant trait database - enhanced coverage and open access

Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Orbit Determination of Close Binary Systems using Lucky Imaging

We present relative positions of visual binaries observed during 2009 with the FastCam "lucky-imaging" camera at the 1.5-m Carlos Sanchez Telescope (TCS) at the Observatorio del Teide. We obtained 424 CCD observations (averaged in 198 mean relative positions) of 157 binaries with angular separations in the range 0.14-15.40", with a median separation of 0.51". For a given system, each CCD image represents the sum of the best 10-25% images from 1000-5000 short-exposure frames. Derived internal errors were 7 mas in r and 1.2^{\circ} (9 mas) in q. When comparing to systems with very well-known orbits, we find that the rms deviation in r residuals is 23 mas, while the rms deviation in q residuals is 0.73 deg/r. We confirmed 18 Hipparcos binaries and we report new companions to BVD 36 A and J 621 B. For binaries with preliminary orbital parameters, the relative radial velocity was estimated as well. We also present four new revised orbits computed for LDS 873, BU 627 A-BC, BU 628 and HO 197 AB. This work is the first results on visual binaries using the FastCam lucky-imaging camera.Comment: 23 pages, 10 figures, 14 tables, accepted August 18th, 2011, to be published in MNRA

High Bandwidth Synaptic Communication and Frequency Tracking in Human Neocortex

Neuronal firing, synaptic transmission, and its plasticity form the building blocks for processing and storage of information in the brain. It is unknown whether adult human synapses are more efficient in transferring information between neurons than rodent synapses. To test this, we recorded from connected pairs of pyramidal neurons in acute brain slices of adult human and mouse temporal cortex and probed the dynamical properties of use-dependent plasticity. We found that human synaptic connections were purely depressing and that they recovered three to four times more swiftly from depression than synapses in rodent neocortex. Thereby, during realistic spike trains, the temporal resolution of synaptic information exchange in human synapses substantially surpasses that in mice. Using information theory, we calculate that information transfer between human pyramidal neurons exceeds that of mouse pyramidal neurons by four to nine times, well into the beta and gamma frequency range. In addition, we found that human principal cells tracked fine temporal features, conveyed in received synaptic inputs, at a wider bandwidth than for rodents. Action potential firing probability was reliably phase-locked to input transients up to 1,000 cycles/s because of a steep onset of action potentials in human pyramidal neurons during spike trains, unlike in rodent neurons. Our data show that, in contrast to the widely held views of limited information transfer in rodent depressing synapses, fast recovering synapses of human neurons can actually transfer substantial amounts of information during spike trains. In addition, human pyramidal neurons are equipped to encode high synaptic information content. Thus, adult human cortical microcircuits relay information at a wider bandwidth than rodent microcircuits

Experimental ovine toxoplasmosis: influence of the gestational stage on the clinical course, lesion development and parasite distribution

P. 1-14The relation between gestational age and foetal death risk in ovine toxoplasmosis is already known, but the mechanisms involved are not yet clear. In order to study how the stage of gestation influences these mechanisms, pregnant sheep of the same age and genetic background were orally dosed with 50 oocysts of Toxoplasma gondii (M4 isolate) at days 40 (G1), 90 (G2) and 120 (G3) of gestation. In each group, four animals were culled on the second, third and fourth week post infection (pi) in order to evaluate parasite load and distribution, and lesions in target organs. Ewes from G1 showed a longer period of hyperthermia than the other groups. Abortions occurred in all groups. While in G2 they were more frequent during the acute phase of the disease, in G3 they mainly occurred after day 20 pi. After challenge, parasite and lesions in the placentas and foetuses were detected from day 19 pi in G3 while in G2 or G1 they were only detected at day 26 pi. However, after initial detection at day 19 pi, parasite burden, measured through RT-PCR, in placenta or foetus of G3 did not increase significantly and, at in the third week pi it was lower than that measured in foetal liver or placenta from G1 to G3 respectively. These results show that the period of gestation clearly influences the parasite multiplication and development of lesions in the placenta and foetus and, as a consequence, the clinical course in ovine toxoplasmosis.S

Possible mechanisms of host resistance to Haemonchus contortus infection in sheep breeds native to the Canary Islands

Haemonchus contortus appears to be the most economically important helminth parasite for small ruminant production in many regions of the world. The two sheep breeds native to the Canary Islands display distinctly different resistant phenotypes under both natural and experimental infections. Canaria Hair Breed (CHB) tends to have significantly lower worm burden and delayed and reduced egg production than the susceptible Canaria Sheep (CS). To understand molecular mechanisms underlying host resistance, we compared the abomasal mucosal transcriptome of the two breeds in response to Haemonchus infection using RNAseq technology. The transcript abundance of 711 and 50 genes were significantly impacted by infection in CHB and CS, respectively (false discovery rate <0.05) while 27 of these genes were significantly affected in both breeds. Likewise, 477 and 16 Gene Ontology (GO) terms were significantly enriched in CHB and CS, respectively (P < 1.0 × 10(−4)). A broad range of mechanisms have evolved in resistant CHB to provide protection against the parasite. Our findings suggest that readily inducible acute inflammatory responses, complement activation, accelerated cell proliferation and subsequent tissue repair, and immunity directed against parasite fecundity all contributed to the development of host resistance to parasitic infection in the resistant breed

Controle massal da broca‑do‑café com armadilhas de garrafa Pet vermelha em cafeeiro

The objective of this work was to evaluate the efficacy of red PET‑bottle traps, containing attractive alcoholic volatile compounds, in the mass control of coffee berry borer (Hypothenemus hampei). The evaluations were carried out in four coffee crops, during two years. Nine hundred PET‑bottle traps, painted red, were distributed in three of the four coffee crops; one crop without traps was used as a control. Holes drilled into berries (%) were determined in these crops. There was a reduction in drilled berries in crops with traps, from the 2007/2008 harvest to the 2008/2009 harvest. The highest coffee berry borer densities in traps was observed in the flowering and small‑green berries stages. The red PET‑bottle trap is efficient in the mass control of the coffee berry borer population, since it reduces the percentage of drilled berries in 57%; however, this reduction is not enough to keep borer densities below the control level.O objetivo deste trabalho foi avaliar a eficácia de armadilha de garrafa Pet vermelha contendo compostos voláteis alcoólicos atrativos, no controle massal da broca‑do‑café (Hypothenemus hampei). As avaliações foram realizadas em quatro lavouras de café, durante dois anos. Foram distribuídas 900 armadilhas de garrafa Pet, pintadas de vermelho, em três das quatro lavouras; uma lavoura sem armadilhas foi usada como controle. O broqueamento dos frutos (%) foi determinado nessas lavouras. Houve redução do broqueamento de frutos nas lavouras com armadilhas, da safra 2007/2008 para a safra 2008/2009. As maiores densidades da broca‑do‑café nas armadilhas foram observadas nos estádios de floração e de frutos chumbinho. A armadilha de garrafa Pet vermelha é eficaz no controle massal da população da broca‑do‑café, por reduzir a percentagem de frutos broqueados em 57%; entretanto, essa redução não é suficiente para manter as densidades da broca abaixo do nível de controle