161 research outputs found
An adenovirus-based vaccine manufacturing technology platform for mucosal or parenteral immunization against poultry diseases in sub-Saharan Africa
Developing novel vaccine technology platforms to respond to emergency situations such as pandemic threats or zoonotic diseases is a worldwide high priority. Public health at a global scale is frequently influenced by the risk of transmission of infectious diseases from wildlife and domestic animals. Thus, veterinary vaccination and animal health monitoring are highly relevant for the deployment of a preventative global policy in the context of âone world, one healthâ.
In regions such as Sub-Saharan Africa, farmersâ activities are frequently affected by the impact of diseases in poultry such as avian influenza and Newcastle disease (ND). ND is one of the most critical, with several outbreaks per year. Currently, protection is provided by vaccination with live vaccines produced only in embryonated eggs, with limitations related to egg supply and the possibility of virus shedding by vaccinated poultry, leading to disease in non-vaccinated birds.
The purpose of this work was to develop an adenovirus (Ad) vectored vaccine platform technology suitable for the rapid adaptation to ND or other avian viral threats. The project involved the phylogenetic analysis of local isolates of Newcastle disease virus (NDV) and the construction of adenoviral vectors expressing the F and HN antigens from NDV genotype VI, either as individual antigens or in bicistronic vectors. Remarkably, adenoviral rescue and generation of primary stocks was streamlined by developing a novel procedure for single step amplification in suspension cultures.
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Data-based investigation of the effects of canopy structure and shadows on chlorophyll fluorescence in a deciduous oak forest
Data from satellite, aircraft, drone, and ground-based measurements have already shown that canopy-scale sun-induced chlorophyll fluorescence (SIF) is tightly related to photosynthesis, which is linked to vegetation carbon assimilation. However, our ability to effectively use those findings are hindered by confounding factors, including canopy structure, fluctuations in solar radiation, and sunâcanopy geometry that highly affect the SIF signal. Thus, disentangling these factors has become paramount in order to use SIF for monitoring vegetation functioning at the canopy scale and beyond. Active chlorophyll fluorescence measurements (FyieldLIF), which directly measures the apparent fluorescence yield, have been widely used to detect physiological variation of the vegetation at the leaf scale. Recently, the measurement of FyieldLIF has become feasible at the canopy scale, opening up new opportunities to decouple structural, biophysical, and physiological components of SIF at the canopy scale. In this study, based on top-of-canopy measurements above a mature deciduous forest, reflectance (R), SIF, SIF normalized by incoming photosynthetically active radiation (SIFy), FyieldLIF, and the ratio between SIFy and FyieldLIF (named Ίk) were used to investigate the effects of canopy structure and shadows on the diurnal and seasonal dynamics of SIF. Further, random forest (RF) models were also used to not only predict FyieldLIF and Ίk, but also provide an interpretation framework by considering additional variables, including the R in the blue, red, green, red-edge, and near-infrared bands; SIF; SIFy; and solar zenith angle (SZA) and solar azimuth angle (SAA). Results revealed that the SIF signal is highly affected by the canopy structure and sunâcanopy geometry effects compared to FyieldLIF. This was evidenced by the weak correlations obtained between SIFy and FyieldLIF at the diurnal timescale. Furthermore, the daily mean SIFâŸy captured the seasonal dynamics of daily mean FâŸyieldLIF and explained 58â% of its variability. The findings also revealed that reflectance in the near-infrared (R-NIR) and the NIRv (the product of R-NIR and normalized difference vegetation index (NDVI)) are good proxies of Ίk at the diurnal timescale, while their correlations with Ίk decrease at the seasonal timescale. With FyieldLIF and Ίk as outputs and the abovementioned variables as predictors, this study also showed that the RF models can explain between 86â% and 90â% of FyieldLIF, as well as 60â% and 70â% of Ίk variations under clear-sky conditions. In addition, the predictor importance estimates for FyieldLIF RF models revealed that R at 410, 665, 740, and 830ânm; SIF; SIFy; SZA; and SAA emerged as the most useful and influential factors for predicting FyieldLIF, while R at 410, 665, 705, and 740ânm; SZA; and SAA are crucial for predicting Ίk. This study highlighted the complexity of interpreting diurnal and seasonal dynamics of SIF in forest canopies. These dynamics are highly dependent on the complex interactions between the structure of the canopy, the vegetation biochemical properties, the illumination angles (SZA and SAA), and the light conditions (ratio of diffuse to direct solar radiation). However, such measurements are necessary to better separate the variability in SIF attributable to radiation and measurement conditions from the subtler variability attributable to plant physiological processes.</p
Physical, mechanical and hygrothermal properties of lateritic building stones (LBS) from Burkina Faso
International audienc
Molecular analysis of Mycobacterium isolates from extrapulmonary specimens obtained from patients in Mexico
<p>Abstract</p> <p>Background</p> <p>Little information is available on the molecular epidemiology in Mexico of <it>Mycobacterium </it>species infecting extrapulmonary sites in humans. This study used molecular methods to determine the <it>Mycobacterium </it>species present in tissues and body fluids in specimens obtained from patients in Mexico with extrapulmonary disease.</p> <p>Methods</p> <p>Bacterial or tissue specimens from patients with clinical or histological diagnosis of extrapulmonary tuberculosis were studied. DNA extracts from 30 bacterial cultures grown in Löwenstein Jensen medium and 42 paraffin-embedded tissues were prepared. Bacteria were cultured from urine, cerebrospinal fluid, pericardial fluid, gastric aspirate, or synovial fluid samples. Tissues samples were from lymph nodes, skin, brain, vagina, and peritoneum. The DNA extracts were analyzed by PCR and by line probe assay (INNO-LiPA MYCOBACTERIA v2. Innogenetics NV, Gent, Belgium) in order to identify the <it>Mycobacterium </it>species present. DNA samples positive for <it>M. tuberculosis </it>complex were further analyzed by PCR and line probe assay (INNO-LiPA Rif.TB, Innogenetics NV, Gent, Belgium) to detect mutations in the <it>rpo</it>B gene associated with rifampicin resistance.</p> <p>Results</p> <p>Of the 72 DNA extracts, 26 (36.1%) and 23 (31.9%) tested positive for <it>Mycobacterium species </it>by PCR or line probe assay, respectively. In tissues, <it>M. tuberculosis </it>complex and <it>M. genus </it>were found in lymph nodes, and <it>M. genus </it>was found in brain and vagina specimens. In body fluids, <it>M. tuberculosis </it>complex was found in synovial fluid. <it>M. gordonae</it>, <it>M. smegmatis</it>, <it>M. kansasii</it>, <it>M. genus</it>, <it>M. fortuitum/M. peregrinum </it>complex and <it>M. tuberculosis </it>complex were found in urine. <it>M. chelonae/M. abscessus </it>was found in pericardial fluid and <it>M. kansasii </it>was found in gastric aspirate. Two of <it>M. tuberculosis </it>complex isolates were also PCR and LiPA positive for the <it>rpo</it>B gene. These two isolates were from lymph nodes and were sensitive to rifampicin.</p> <p>Conclusion</p> <p>1) We describe the <it>Mycobacterium </it>species diversity in specimens derived from extrapulmonary sites in symptomatic patients in Mexico; 2) Nontuberculous mycobacteria were found in a considerable number of patients; 3) Genotypic rifampicin resistance in <it>M. tuberculosis </it>complex infections in lymph nodes was not found.</p
Towards long-term standardised carbon and greenhouse gas observations for monitoring Europe's terrestrial ecosystems : a review
Research infrastructures play a key role in launching a new generation of integrated long-term, geographically distributed observation programmes designed to monitor climate change, better understand its impacts on global ecosystems, and evaluate possible mitigation and adaptation strategies. The pan-European Integrated Carbon Observation System combines carbon and greenhouse gas (GHG; CO2, CH4, N2O, H2O) observations within the atmosphere, terrestrial ecosystems and oceans. High-precision measurements are obtained using standardised methodologies, are centrally processed and openly available in a traceable and verifiable fashion in combination with detailed metadata. The Integrated Carbon Observation System ecosystem station network aims to sample climate and land-cover variability across Europe. In addition to GHG flux measurements, a large set of complementary data (including management practices, vegetation and soil characteristics) is collected to support the interpretation, spatial upscaling and modelling of observed ecosystem carbon and GHG dynamics. The applied sampling design was developed and formulated in protocols by the scientific community, representing a trade-off between an ideal dataset and practical feasibility. The use of open-access, high-quality and multi-level data products by different user communities is crucial for the Integrated Carbon Observation System in order to achieve its scientific potential and societal value.Peer reviewe
Using digital time-lapse cameras to monitor species-specific understorey and overstorey phenology in support of wildlife habitat assessment
Hardware compression scheme based on low-complexity arithmetic encoding for low power image transmission over WSNs
International audienc
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