1,910 research outputs found
Expression of Interferon Gamma by a Recombinant Rabies Virus Strongly Attenuates the Pathogenicity of the Virus via Induction of Type I Interferon.
UNLABELLED: Previous animal model experiments have shown a correlation between interferon gamma (IFN-γ) expression and both survival from infection with attenuated rabies virus (RABV) and reduction of neurological sequelae. Therefore, we hypothesized that rapid production of murine IFN-γ by the rabies virus itself would induce a more robust antiviral response than would occur naturally in mice. To test this hypothesis, we used reverse engineering to clone the mouse IFN-γ gene into a pathogenic rabies virus backbone, SPBN, to produce the recombinant rabies virus designated SPBNγ. Morbidity and mortality were monitored in mice infected intranasally with SPBNγ or SPBN(-) control virus to determine the degree of attenuation caused by the expression of IFN-γ. Incorporation of IFN-γ into the rabies virus genome highly attenuated the virus. SPBNγ has a 50% lethal dose (LD50) more than 100-fold greater than SPBN(-). In vitro and in vivo mouse experiments show that SPBNγ infection enhances the production of type I interferons. Furthermore, knockout mice lacking the ability to signal through the type I interferon receptor (IFNAR(-/-)) cannot control the SPBNγ infection and rapidly die. These data suggest that IFN-γ production has antiviral effects in rabies, largely due to the induction of type I interferons.
IMPORTANCE: Survival from rabies is dependent upon the early control of virus replication and spread. Once the virus reaches the central nervous system (CNS), this becomes highly problematic. Studies of CNS immunity to RABV have shown that control of replication begins at the onset of T cell entry and IFN-γ production in the CNS prior to the appearance of virus-neutralizing antibodies. Moreover, antibody-deficient mice are able to control but not clear attenuated RABV from the CNS. We find here that IFN-γ triggers the early production of type I interferons with the expected antiviral effects. We also show that engineering a lethal rabies virus to express IFN-γ directly in the infected tissue reduces rabies virus replication and spread, limiting its pathogenicity in normal and immunocompromised mice. Therefore, vector delivery of IFN-γ to the brain may have the potential to treat individuals who would otherwise succumb to infection with rabies virus
Broad-band X-ray spectral evolution of GX 339-4 during a state transition
We report on X-ray and soft gamma-ray observations of the black-hole
candidate GX 339-4 during its 2007 outburst, performed with the RXTE and
INTEGRAL satellites. The hardness-intensity diagram of all RXTE/PCA data
combined shows a q-shaped track similar to that observed in previous
outbursts.The evolution in the diagram suggested that a transition from
hard-intermediate state to soft-intermediate state occurred, simultaneously
with INTEGRAL observations performed in March. The transition is confirmed by
the timing analysis presented in this work, which reveals that a weak type-A
quasi-periodic oscillation (QPO) replaces a strong type-C QPO. At the same
time, spectral analysis shows that the flux of the high-energy component shows
a significant decrease in its flux. However, we observe a delay (roughly one
day) between variations of the spectral parameters of the high-energy component
and changes in the flux and timing properties. The changes in the high-energy
component can be explained either in terms the high-energy cut-off or in terms
of a variations in the reflection component. We compare our results with those
from a similar transition during the 2004 outburst of GX 339-4.Comment: 8 pages, 6 figures, accepted for publication in MNRAS Main Journa
Observations of SN2011fe with INTEGRAL
SN2011fe was detected by the Palomar Transient Factory on August 24th 2011 in
M101 few hours after the explosion. From the early spectra it was immediately
realized that it was a Type Ia supernova thus making this event the brightest
one discovered in the last twenty years. In this paper the observations
performed with the instruments on board of INTEGRAL (SPI, IBIS/ISGRI, JEM-X and
OMC) before and after the maximum of the optical light as well as the
interpretation in terms of the existing models of --ray emission from
such kind of supernovae are reported. All INTEGRAL high-energy have only been
able to provide upper limits to the expected emission due to the decay of
Ni. These bounds allow to reject explosions involving a massive white
dwarf in the sub--Chandrasekhar scenario. On the other hand, the optical light
curve obtained with the OMC camera suggests that the event was produced by a
delayed detonation of a CO white dwarf that produced M of
Ni. In this particular case, INTEGRAL would have only been able to
detect the early --ray emission if the supernova had occurred at a
distance of 2 -3 Mpc, although the brightest event could be visible up to
distances larger by a factor two.Comment: Proceedings of "An INTEGRAL view of the high-energy sky (the first 10
years)" the 9th INTEGRAL Workshop, October 15-19, 2012, Paris, France, in
Proceedings of Science (INTEGRAL 2012), Eds. A. Goldwurm, F. Lebrun and C.
Winkler, http://pos.sissa.it/cgi-bin/reader/conf.cgi?confid=176, id number
PoS (INTEGRAL 2012) 103 (2013
Observation of SN2011fe with INTEGRAL. I. Pre--maximum phase
SN2011fe was detected by the Palomar Transient Factory on August 24th 2011 in
M101 a few hours after the explosion. From the early optical spectra it was
immediately realized that it was a Type Ia supernova thus making this event the
brightest one discovered in the last twenty years. The distance of the event
offered the rare opportunity to perform a detailed observation with the
instruments on board of INTEGRAL to detect the gamma-ray emission expected from
the decay chains of Ni. The observations were performed in two runs, one
before and around the optical maximum, aimed to detect the early emission from
the decay of Ni and another after this maximum aimed to detect the
emission of Co. The observations performed with the instruments on board
of INTEGRAL (SPI, IBIS/ISGRI, JEMX and OMC) have been analyzed and compared
with the existing models of gamma-ray emission from such kind of supernovae. In
this paper, the analysis of the gamma-ray emission has been restricted to the
first epoch. Both, SPI and IBIS/ISGRI, only provide upper-limits to the
expected emission due to the decay of Ni. These upper-limits on the
gamma-ray flux are of 7.1 10 ph/s/cm for the 158 keV line
and of 2.3 10 ph/s/cm for the 812 keV line. These bounds
allow to reject at the level explosions involving a massive white
dwarf, M in the sub--Chandrasekhar scenario and specifically
all models that would have substantial amounts of radioactive Ni in the
outer layers of the exploding star responsible of the SN2011fe event. The
optical light curve obtained with the OMC camera also suggests that SN2011fe
was the outcome of the explosion, possibly a delayed detonation although other
models are possible, of a CO white dwarf that synthesized M
of Ni. For this specific model.Comment: Accepted for publication in A&A. 10 pages, 10 figure
XMM-Newton and INTEGRAL observations of the very faint X-ray transient IGRJ17285-2922/XTEJ1728-295 during the 2010 outburst
We report the first broad-band (0.5-150 keV) simultaneous X-ray observations
of the very faint X-ray transient IGRJ17285-2922/XTEJ1728-295 performed with
XMM-Newton and INTEGRAL satellites during its last outburst, started on 2010,
August 28. XMM-Newton observed the source on 2010 September 9-10, for 22ks.
INTEGRAL observations were part of the publicly available Galactic Bulge
program, and overlapped with the times covered by XMM-Newton. The broad-band
spectroscopy resulted in a best-fit with an absorbed power law displaying a
photon index of 1.61+/-0.01, an absorbing column density of (5.10+/-0.05)E21
cm-2, and a flux of 2.4E-10 erg/cm2/s (1-100 keV), corrected for the
absorption. The data did not require either a spectral cut-off (E>50 keV) or an
additional soft component. The slopes of the XMM-Newton and INTEGRAL separate
spectra were compatible, within the uncertainties. The timing analysis does not
show evidence either for X-ray pulsations or for type I X-ray bursts. The broad
band X-ray spectrum as well as the power density spectrum are indicative of a
low hard state in a low mass X-ray binary, although nothing conclusive can be
said about the nature of the compact object (neutron star or black hole). The
results we are reporting here allow us to conclude that IGRJ17285-2922 is a low
mass X-ray binary, located at a distance greater than 4 kpc.Comment: Accepted for publication in MNRAS; 7 pages, 6 figure, 1 table.
Accepted 2011 April 5. Received 2011 April 5; in original form 2011 February
2
Oscillations in Procyon A: First results from a multi-site campaign
Procyon A is a bright F5IV star in a binary system. Although the distance,
mass and angular diameter of this star are all known with high precision, the
exact evolutionary state is still unclear. Evolutionary tracks with different
ages and different mass fractions of hydrogen in the core pass, within the
errors, through the observed position of Procyon A in the Hertzsprung-Russell
diagram. For more than 15 years several different groups have studied the
solar-like oscillations in Procyon A to determine its evolutionary state.
Although several studies independently detected power excess in the
periodogram, there is no agreement on the actual oscillation frequencies yet.
This is probably due to either insufficient high-quality data (i.e., aliasing)
or due to intrinsic properties of the star (i.e., short mode lifetimes). Now a
spectroscopic multi-site campaign using 10 telescopes world-wide (minimizing
aliasing effects) with a total time span of nearly 4 weeks (increase the
frequency resolution) is performed to identify frequencies in this star and
finally determine its properties and evolutionary state.Comment: 7 pages, 4 figures to be published in the proceedings of HELAS II
International Conference: Helioseismology, Asteroseismology and MHD
Connections published in the Journal of Physics: Conference Series. High
resolution colour figures can be provided on reques
Asteroseismology of Procyon with SOPHIE
This paper reports a 9-night asteroseismic observation program conducted in
January 2007 with the new spectrometer Sophie at the OHP 193-cm telescope, on
the F5 IV-V target Procyon A. This first asteroseismic program with Sophie was
intended to test the performance of the instrument with a bright but demanding
asteroseismic target and was part of a multisite network. The Sophie spectra
have been reduced with the data reduction software provided by OHP. The Procyon
asteroseismic data were then analyzed with statistical tools. The asymptotic
analysis has been conducted considering possible curvature in the echelle
diagram analysis. These observations have proven the efficient performance of
Sophie used as an asteroseismometer, and succeed in a clear detection of the
large spacing. An \'echelle diagram based on the 54-Hz spacing shows clear
ridges. Identification of the peaks exhibits large spacings varying from about
52 Hz to 56 Hz.Comment: 7 pages, 7 figure
Search for Zgamma events with large missing transverse energy in ppbar collisions at sqrt(s)=1.96 TeV
We present the first search for supersymmetry (SUSY) in Zgamma final states
with large missing transverse energy using data corresponding to an integrated
luminosity of 6.2 fb-1 collected with the D0 experiment in ppbar collisions at
sqrt(s)=1.96 TeV. This signature is predicted in gauge-mediated SUSY-breaking
models, where the lightest neutralino is the next-to-lightest supersymmetric
particle (NLSP) and is produced in pairs, possibly through decay from heavier
supersymmetric particles. The NLSP can decay either to a Z boson or a photon
and an associated gravitino that escapes detection. We exclude this model at
the 95% C.L. for SUSY breaking scales of Lambda < 87 TeV, corresponding to
neutralino masses of < 151 GeV.Comment: submitted to Phys. Rev. Let
Search for Decay
We have searched for the charmless hadronic decay of B0 mesons into two
neutral pions. Using 9.13fb^-1 taken at the Upsilon(4S) with the CLEO detector,
we obtain an improved upper limit for the branching fraction BR(B0-->pi0pi0) <
5.7*10^-6 at the 90% confidence level.Comment: pages postscript, also available through
http://w4.lns.cornell.edu/public/CLN
Measurement of Leptonic Asymmetries and Top Quark Polarization in ttbar Production
We present measurements of lepton (l) angular distributions in ttbar -> W+ b
W- b -> l+ nu b l- nubar bbar decays produced in ppbar collisions at a
center-of-mass energy of sqrt(s)=1.96TeV, where l is an electron or muon. Using
data corresponding to an integrated luminosity of 5.4fb^-1, collected with the
D0 detector at the Fermilab Collider, we find that the angular distributions of
l- relative to anti-protons and l+ relative to protons are in agreement with
each other. Combining the two distributions and correcting for detector
acceptance we obtain the forward-backward asymmetry A^l_FB = (5.8 +- 5.1(stat)
+- 1.3(syst))%, compared to the standard model prediction of A^l_FB (predicted)
= (4.7 +- 0.1)%. This result is further combined with the measurement based on
the analysis of the l+jets final state to obtain A^l_FB = (11.8 +- 3.2)%.
Furthermore, we present a first study of the top-quark polarization.Comment: submitted versio
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