Selection Models for the Internal Quality of Fruit, based on Time Domain Laser Reflectance Spectroscopy

Time domain laser reflectance spectroscopy (TRS) was applied for the first time to evaluate internal fruit quality. This technique, known in medicine-related knowledge areas, has not been used before in agricultural or food research. It allows the simultaneous measurement of two optical characteristics of the sample: light scattering inside the tissues and light absorption. Models to estimate non-destructively firmness, soluble solids and acid contents in tomato, apple, peach and nectarine were developed using sequential statistical techniques: principal component analysis, multiple stepwise linear regression, clustering and discriminant analysis. Consistent correlations were established between the two parameters measured with TRS, i.e. absorption and transport scattering coefficients, with chemical constituents (soluble solids and acids) and firmness, respectively. Classification models were created to sort fruits into three quality grades (‘low’, ‘medium’ and ‘high’), according to their firmness, soluble solids and acidity

Notes on the larval habitat preference and microdistribution of Potamophylax rotundipennis (Insecta: Trichoptera) in a stream reach of the Börzsöny Mountains (Northern Hungary)

Larval habitat preference of Potamophylax rotundipennis (Insecta: Trichoptera) was examined under natural conditions. The result of this study indicated a high preference for stone to sand and detritus, and preference for the low depth of water. This preference resulted that P. rotundipennis larvae showed a homogenous distribution along the stream-reach, but not across it

WALLER CREEK STATUS REPORT 2002

This short report contains information on Waller Creek's estimated population, fecal coliform levels, land use distribution, mean baseflow, total phosphorus, and other water quality indicators.Water chemistry and biological data from Waller Creek were reviewed in order to evaluate temporal and spatial trends on this urban stream. The results of site analysis of variance indicated little discernable difference among sites and variability was high for conventional water chemistry constituents. Temporal trends were inconsistent with no notable degradation or improvements. Benthic macroinvertebrate surveys showed some significant site differences that appear to relate primarily to flow and possibly to nutrient enrichment. These surveys documented decreasing water quality over time; however, this result is apparently an artifact of the final survey, which took place after a long dry period. Overall, Waller Creek has low water quality and biological integrity when compared to other Austin area streams (worse than 75 percent of the watersheds for most measures).Waller Creek Working Grou

Global distribution of two fungal pathogens threatening endangered sea turtles

This work was supported by grants of Ministerio de Ciencia e Innovación, Spain (CGL2009-10032, CGL2012-32934). J.M.S.R was supported by PhD fellowship of the CSIC (JAEPre 0901804). The Natural Environment Research Council and the Biotechnology and Biological Sciences Research Council supported P.V.W. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Thanks Machalilla National Park in Ecuador, Pacuare Nature Reserve in Costa Rica, Foundations Natura 2000 in Cape Verde and Equilibrio Azul in Ecuador, Dr. Jesus Muñoz, Dr. Ian Bell, Dr. Juan Patiño for help and technical support during samplingPeer reviewedPublisher PD

Comparison of species richness of light trap-collected caddisfly assemblages (Insecta: Trichoptera) using rarefaction

ConselVation value of light trap-collected caddisfly assemblages(Insecta: Trichoptera) was evaluated on the basis of their species richness. The assemblage coming from an artificial stream showed a higher conservation value based on species richness than the natural ones. In contrast, using rarefaction, the conservation value of the assemblage in the artificial stream was lower in comparison with assemblages in natural habitats. Further examples are given to demonstrate the importance of rarefaction in comparing species richness of assemblages

Deriving Biomass Models for Small-Diameter Loblolly Pine on the Crossett Experimental Forest

Foresters and landowners have a growing interest in carbon sequestration and cellulosic biofuels in southern pine forests, and hence need to be able to accurately predict them. To this end, we derived a set of aboveground biomass models using data from 62 small-diameter loblolly pines (Pinus taeda) sampled on the Crossett Experimental Forest in southeastern Arkansas. Of the 25 equations initially evaluated, we chose 17 that best fit our dataset and compared them using a suite of conventional test statistics, including pseudo-R2 , root mean squared error (RMSE), and bias. Because most of the 17 models varied little in pseudoR 2 (ranging between 0.96 and 0.99), bias (all were within ± 0.01), and RMSE, an additional comparison was done using Akaike’s Information Criterion corrected for small sample size (AICc). This test statistic produced considerably more discrimination between the biomass models. Of the 17 models evaluated, six produced ΔAICc scores that met or exceeded the threshold for substantial support. To recommend a single preferred model, we then extrapolated beyond our actual data and qualitatively compared model predictions with those from the National Biomass Estimator. Our “best” model did not have the minimum AICc score, but rather predicted logically consistent aboveground biomass values at both the upper and lower ends of our extrapolation

ALTERNATIVE CYCLING STRATEGIES FOR SHRIMP FARMING IN ARID ZONES OF MEXICO: DEALING WITH RISK AND UNCERTAINTY

Northwest Mexican coastal waters have large seasonal temperature variations, high salinity, and are subject to intense solar radiation. Shrimp farms in this region have been using two annual production strategies; six- to eight-month cycle with one complete harvest and several partial harvests, or two, three- to four-month cycles with complete harvests. The preferred strategy depends on two uncertain variables; shrimp growth, which varies across the region, and market price, which varies across the season. A bioeconomic model was used to compare the economic yield of the two cycling strategies for three zones across the region, under three alternative average annual temperatures states. Simple decision theory criteria are used to show that the two-cycle strategy dominates the one-cycle strategy in the Bahia de La Paz zone. Results for central and northern Sonora are conditional on temperature.Resource /Energy Economics and Policy,

Resistance of Rice Varieties to \u3ci\u3eSitophilus oryzae\u3c/i\u3e (Coleoptera: Curculionidae)

Abstract Rice, Oryza sativa L. (Poaceae), is one of the world’s most important food crops. Among the insects that damage rice grains, the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), is the most important species. The objective of this study was to identify rice varieties with resistance to S. oryzae as measured by grain feeding damage and S. oryzae reproduction. The rice varieties evaluated were obtained from the germplasm bank of the National Center of Research Rice and Beans, Embrapa, Brazil. The experimental design was completely randomized with 3 replications and 22 treatments (varieties). The varieties Bonança, Esmeralda, and Rio Verde had the antibiosis and/or antixenosis type of resistance, providing high rates of mortality, low rates of adult emergence, and low rates of grain consumption by S. oryzae. The varieties Pepita and Progresso were susceptible, with a high rate of S. oryzae adult emergence and grain consumption. The presence or absence of a fissure in the grain was a major morphological characteristic determining susceptibility or resistance to S. oryzae in rice varieties. Resumo O arroz (Oryza sativa L.; Poaceae) é uma das culturas alimentares mais importantes do mundo. Entre os insetos que danificam os grãos de arroz, o gorgulho do arroz Sitophylus oryzae L. (Coleoptera: Curculionidae) é a espécia mais importante. O objetivo deste trabalho foi identificar variedades de arroz com resistência a S. oryzae. As variedades de arroz avaliadas foram obtidas do banco de germoplasma do Centro Nacional de Pesquisas em Arroz e Feijão, EMBRAPA, Brasil. O delineamento experimental foi inteiramente casualizado com 3 repetições e 22 tratamentos (variedades). As variedades Bonança, Esmeralda e Rio Verde apresentaram resistência do tipo antibiose e/ou antixenose, tendo altas taxas de mortalidade, baixas taxas de emergência de adultos e baixo consumo de grãos por S. oryzae. As variedades Pepita e Progresso são suscetíveis com alta emergência de adultos e consumo de grãos por S. oryzae. A presença ou ausência de fissuras no grão é uma importante característica morfológica para determinar a susceptibilidade ou resistência a S. oryzae em variedades de arroz

Identification and characterization of dysregulated P-element induced wimpy testis-interacting RNAs in head and neck squamous cell carcinoma.

It is clear that alcohol consumption is a major risk factor in the pathogenesis of head and neck squamous cell carcinoma (HNSCC); however, the molecular mechanism underlying the pathogenesis of alcohol-associated HNSCC remains poorly understood. The aim of the present study was to identify and characterize P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) and PIWI proteins dysregulated in alcohol-associated HNSCC to elucidate their function in the development of this cancer. Using next generation RNA-sequencing (RNA-seq) data obtained from 40 HNSCC patients, the piRNA and PIWI protein expression of HNSCC samples was compared between alcohol drinkers and non-drinkers. A separate piRNA expression RNA-seq analysis of 18 non-smoker HNSCC patients was also conducted. To verify piRNA expression, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed on the most differentially expressed alcohol-associated piRNAs in ethanol and acetaldehyde-treated normal oral keratinocytes. The correlation between piRNA expression and patient survival was analyzed using Kaplan-Meier estimators and multivariate Cox proportional hazard models. A comparison between alcohol drinking and non-drinking HNSCC patients demonstrated that a panel of 3,223 piRNA transcripts were consistently detected and differentially expressed. RNA-seq analysis and in vitro RT-qPCR verification revealed that 4 of these piRNAs, piR-35373, piR-266308, piR-58510 and piR-38034, were significantly dysregulated between drinking and non-drinking cohorts. Of these four piRNAs, low expression of piR-58510 and piR-35373 significantly correlated with improved patient survival. Furthermore, human PIWI-like protein 4 was consistently upregulated in ethanol and acetaldehyde-treated normal oral keratinocytes. These results demonstrate that alcohol consumption may cause dysregulation of piRNA expression in HNSCC and in vitro verifications identified 4 piRNAs that may be involved in the pathogenesis of alcohol-associated HNSCC

Garbage in the diet of carnivores in an agricultural area

Human food waste is considered to be richer in carbohydrates, lipids and proteins than most natural food supplies; however, it is very well digested in scats. So, as an indication of this kind of food in the diet, we have used each indigestible, anthropogenic origin element found in faeces (e.g., glass, plastic, rubber, etc.). There are few studies discussing the importance of garbage in the diet of mammalian predators living in farmland; definitely,  most focus on this issue in urban areas. We studied the contribution of garbage in the diet of raccoon dog  (Nyctereutes procyonoides), red fox (Vulpes vulpes), marten (Martes sp.), polecat (Mustela putorius), stoat (Mustela erminea), American mink (Neovison vison) and Eurasian otter (Lutra lutra) in the agricultural areas of western Poland in 2006-2010. In addition, we examined the spatial changes in the diet of red fox and polecat. The largest contribution of garbage was found in scats of raccoon dog (8.8%), red fox (4.8%) and marten (4.3%). The diet of polecat, stoat and Eurasian otter contained 2.5%, 1.7% and 0.2% garbage items respectively. The most frequent item was plastic. Our analysis showed that garbage consumption by red fox and polecat was greater  closer to human settlements. The results reveal a continuous gradient in the garbage consumption that corresponds with the degree of synanthropization of particular species