The design and evaluation of interfaces for navigating gigapixel images in digital pathology

This paper describes the design and evaluation of two generations of an interface for navigating datasets of gigapixel images that pathologists use to diagnose cancer. The interface design is innovative because users panned with an overview:detail view scale difference that was up to 57 times larger than established guidelines, and 1 million pixel ‘thumbnail’ overviews that leveraged the real-estate of high resolution workstation displays. The research involved experts performing real work (pathologists diagnosing cancer), using datasets that were up to 3150 times larger than those used in previous studies that involved navigating images. The evaluation provides evidence about the effectiveness of the interfaces, and characterizes how experts navigate gigapixel images when performing real work. Similar interfaces could be adopted in applications that use other types of high-resolution images (e.g., remote sensing or highthroughput microscopy)

Scale Stain: Multi-Resolution Feature Enhancement in Pathology Visualization

Digital whole-slide images of pathological tissue samples have recently become feasible for use within routine diagnostic practice. These gigapixel sized images enable pathologists to perform reviews using computer workstations instead of microscopes. Existing workstations visualize scanned images by providing a zoomable image space that reproduces the capabilities of the microscope. This paper presents a novel visualization approach that enables filtering of the scale-space according to color preference. The visualization method reveals diagnostically important patterns that are otherwise not visible. The paper demonstrates how this approach has been implemented into a fully functional prototype that lets the user navigate the visualization parameter space in real time. The prototype was evaluated for two common clinical tasks with eight pathologists in a within-subjects study. The data reveal that task efficiency increased by 15% using the prototype, with maintained accuracy. By analyzing behavioral strategies, it was possible to conclude that efficiency gain was caused by a reduction of the panning needed to perform systematic search of the images. The prototype system was well received by the pathologists who did not detect any risks that would hinder use in clinical routine

Exploratory Visualization of Astronomical Data on Ultra-high-resolution Wall Displays

International audienceUltra-high-resolution wall displays feature a very high pixel density over a large physical surface, which makes them well-suited to the collaborative, exploratory visualization of large datasets. We introduce FITS-OW, an application designed for such wall displays, that enables astronomers to navigate in large collections of FITS images, query astronomical databases, and display detailed, complementary data and documents about multiple sources simultaneously. We describe how astronomers interact with their data using both the wall's touch-sensitive surface and handheld devices. We also report on the technical challenges we addressed in terms of distributed graphics rendering and data sharing over the computer clusters that drive wall displays

Faster than light (microscopy): superiority of digital pathology over microscopy for assessment of immunohistochemistry

YesDigital pathology offers the potential for significant benefits in diagnostic pathology, but currently the efficiency of slide viewing is a barrier to adoption. We hypothesised that presenting digital slides for simultaneous viewing of multiple sections of tissue for comparison, as in those with immunohistochemical panels, would allow pathologists to review cases more quickly. Novel software was developed to view synchronised parallel tissue sections on a digital pathology workstation. Sixteen histopathologists reviewed three liver biopsy cases including an immunohistochemical panel using the digital microscope, and three different liver biopsy cases including an immunohistochemical panel using the light microscope. The order of cases and interface was fully counterbalanced. Time to diagnosis was recorded and mean times are presented as data approximated to a normalised distribution. Mean time to diagnosis was 4 min 3 s using the digital microscope and 5 min 24 s using the light microscope, saving 1 min 21 s (95% CI 16 s to 2 min 26 s; p=0.02), using the digital microscope. Overall normalised mean time to diagnosis was 85% on the digital pathology workstation compared with 115% on the microscope, a relative reduction of 26%. With appropriate interface design, it is quicker to review immunohistochemical slides using a digital microscope than the conventional light microscope, without incurring any major diagnostic errors. As digital pathology becomes more integrated with routine clinical workflow and pathologists increase their experience of the technology, it is anticipated that other tasks will also become more time-efficient

KOLAM : human computer interfaces fro visual analytics in big data imagery

In the present day, we are faced with a deluge of disparate and dynamic information from multiple heterogeneous sources. Among these are the big data imagery datasets that are rapidly being generated via mature acquisition methods in the geospatial, surveillance (specifically, Wide Area Motion Imagery or WAMI) and biomedical domains. The need to interactively visualize these imagery datasets by using multiple types of views (as needed) into the data is common to these domains. Furthermore, researchers in each domain have additional needs: users of WAMI datasets also need to interactively track objects of interest using algorithms of their choice, visualize the resulting object trajectories and interactively edit these results as needed. While software tools that fulfill each of these requirements individually are available and well-used at present, there is still a need for tools that can combine the desired aspects of visualization, human computer interaction (HCI), data analysis, data management, and (geo-)spatial and temporal data processing into a single flexible and extensible system. KOLAM is an open, cross-platform, interoperable, scalable and extensible framework for visualization and analysis that we have developed to fulfil the above needs. The novel contributions in this thesis are the following: 1) Spatio-temporal caching for animating both giga-pixel and Full Motion Video (FMV) imagery, 2) Human computer interfaces purposefully designed to accommodate big data visualization, 3) Human-in-the-loop interactive video object tracking - ground-truthing of moving objects in wide area imagery using algorithm assisted human-in-the-loop coupled tracking, 4) Coordinated visualization using stacked layers, side-by-side layers/video sub-windows and embedded imagery, 5) Efficient one-click manual tracking, editing and data management of trajectories, 6) Efficient labeling of image segmentation regions and passing these results to desired modules, 7) Visualization of image processing results generated by non-interactive operators using layers, 8) Extension of interactive imagery and trajectory visualization to multi-monitor wall display environments, 9) Geospatial applications: Providing rapid roam, zoom and hyper-jump spatial operations, interactive blending, colormap and histogram enhancement, spherical projection and terrain maps, 10) Biomedical applications: Visualization and target tracking of cell motility in time-lapse cell imagery, collecting ground-truth from experts on whole-slide imagery (WSI) for developing histopathology analytic algorithms and computer-aided diagnosis for cancer grading, and easy-to-use tissue annotation features.Includes bibliographical reference

Interactive Visualization of 3D Histopathology in Native Resolution

We present a visualization application that enables effective interactive visual analysis of large-scale 3D histopathology, that is, high-resolution 3D microscopy data of human tissue. Clinical work flows and research based on pathology have, until now, largely been dominated by 2D imaging. As we will show in the paper, studying volumetric histology data will open up novel and useful opportunities for both research and clinical practice. Our starting point is the current lack of appropriate visualization tools in histopathology, which has been a limiting factor in the uptake of digital pathology. Visualization of 3D histology data does pose difficult challenges in several aspects. The full-color datasets are dense and large in scale, on the order of 100,000 × 100,000× 100 voxels. This entails serious demands on both rendering performance and user experience design. Despite this, our developed application supports interactive study of 3D histology datasets at native resolution. Our application is based on tailoring and tuning of existing methods, system integration work, as well as a careful study of domain specific demands emanating from a close participatory design process with domain experts as team members. Results from a user evaluation employing the tool demonstrate a strong agreement among the 14 participating pathologists that 3D histopathology will be a valuable and enabling tool for their work

Multimodal Biomedical Data Visualization: Enhancing Network, Clinical, and Image Data Depiction

In this dissertation, we present visual analytics tools for several biomedical applications. Our research spans three types of biomedical data: reaction networks, longitudinal multidimensional clinical data, and biomedical images. For each data type, we present intuitive visual representations and efficient data exploration methods to facilitate visual knowledge discovery. Rule-based simulation has been used for studying complex protein interactions. In a rule-based model, the relationships of interacting proteins can be represented as a network. Nevertheless, understanding and validating the intended behaviors in large network models are ineffective and error prone. We have developed a tool that first shows a network overview with concise visual representations and then shows relevant rule-specific details on demand. This strategy significantly improves visualization comprehensibility and disentangles the complex protein-protein relationships by showing them selectively alongside the global context of the network. Next, we present a tool for analyzing longitudinal multidimensional clinical datasets, that we developed for understanding Parkinson's disease progression. Detecting patterns involving multiple time-varying variables is especially challenging for clinical data. Conventional computational techniques, such as cluster analysis and dimension reduction, do not always generate interpretable, actionable results. Using our tool, users can select and compare patient subgroups by filtering patients with multiple symptoms simultaneously and interactively. Unlike conventional visualizations that use local features, many targets in biomedical images are characterized by high-level features. We present our research characterizing such high-level features through multiscale texture segmentation and deep-learning strategies. First, we present an efficient hierarchical texture segmentation approach that scales up well to gigapixel images to colorize electron microscopy (EM) images. This enhances visual comprehensibility of gigapixel EM images across a wide range of scales. Second, we use convolutional neural networks (CNNs) to automatically derive high-level features that distinguish cell states in live-cell imagery and voxel types in 3D EM volumes. In addition, we present a CNN-based 3D segmentation method for biomedical volume datasets with limited training samples. We use factorized convolutions and feature-level augmentations to improve model generalization and avoid overfitting