Further investigation of white dwarfs in the open clusters NGC2287 and NGC3532

We report the results of a CCD imaging survey, complimented by astrometric and spectroscopic follow-up studies, that aims to probe the fate of heavy-weight intermediate mass stars by unearthing new, faint, white dwarf members of the rich, nearby, intermediate age open clusters NGC3532 and NGC2287. We identify a total of four white dwarfs with distances, proper motions and cooling times which can be reconciled with membership of these populations. We find that WDJ0643-203 in NGC2287, with an estimated mass of M=1.02-1.16Msun, is potentially the most massive white dwarf so far identified within an open cluster. Guided by the predictions of modern theoretical models of the late-stage evolution of heavy-weight intermediate mass stars, we conclude that there is a distinct possibility it has a core composed of O and Ne. We also determine that despite the cooling times of the three new white dwarfs in NGC3532 and the previously known degenerate member NGC3532-10 spanning ~90Myr, they all have remarkably similar masses (M~0.9-1Msun). This is fully consistent with the results from our prior work on a heterogeneous sample of ~50 white dwarfs from 12 stellar populations, on the basis of which we argued that the stellar initial mass-final mass relation is less steep at Minit>4Msun than in the adjacent lower initial mass regime. This change in the gradient of the relation could account for the secondary peak observed in the mass distribution of the field white dwarf population and mitigate the need to invoke close binary evolution to explain its existence. Spectroscopic investigation of numerous additional candidate white dwarf members of NGC3532 unearthed by a recent independent study would be useful to confirm (or otherwise) these conclusions.Comment: 8 Figures, 8 tables. Accepted for publication in MNRA

Shedding light on cell compartmentation in the candidate phylum Poribacteria by high resolution visualisation and transcriptional profiling

Assigning functions to uncultivated environmental microorganisms continues to be a challenging endeavour. Here, we present a new microscopy protocol for fluorescence in situ hybridisation-correlative light and electron microscopy (FISH-CLEM) that enabled, to our knowledge for the first time, the identification of single cells within their complex microenvironment at electron microscopy resolution. Members of the candidate phylum Poribacteria, common and uncultivated symbionts of marine sponges, were used towards this goal. Cellular 3D reconstructions revealed bipolar, spherical granules of low electron density, which likely represent carbon reserves. Poribacterial activity profiles were retrieved from prokaryotic enriched sponge metatranscriptomes using simulation-based optimised mapping. We observed high transcriptional activity for proteins related to bacterial microcompartments (BMC) and we resolved their subcellular localisation by combining FISH-CLEM with immunohistochemistry (IHC) on ultra-thin sponge tissue sections. In terms of functional relevance, we propose that the BMC-A region may be involved in 1,2-propanediol degradation. The FISH-IHC-CLEM approach was proven an effective toolkit to combine -omics approaches with functional studies and it should be widely applicable in environmental microbiology

A new model of cosmogenic production of radiocarbon 14C in the atmosphere

We present the results of full new calculation of radiocarbon 14C production in the Earth atmosphere, using a numerical Monte-Carlo model. We provide, for the first time, a tabulated 14C yield function for the energy of primary cosmic ray particles ranging from 0.1 to 1000 GeV/nucleon. We have calculated the global production rate of 14C, which is 1.64 and 1.88 atoms/cm2/s for the modern time and for the pre-industrial epoch, respectively. This is close to the values obtained from the carbon cycle reservoir inventory. We argue that earlier models overestimated the global 14C production rate because of outdated spectra of cosmic ray heavier nuclei. The mean contribution of solar energetic particles to the global 14C is calculated as about 0.25% for the modern epoch. Our model provides a new tool to calculate the 14C production in the Earth's atmosphere, which can be applied, e.g., to reconstructions of solar activity in the past.Comment: Published in EPSL, 337, 114, 201

Neutron monitors and muon detectors for solar modulation studies: Interstellar flux, yield function, and assessment of critical parameters in count rate calculations

Particles count rates at given Earth location and altitude result from the convolution of (i) the interstellar (IS) cosmic-ray fluxes outside the solar cavity, (ii) the time-dependent modulation of IS into Top-of-Atmosphere (TOA) fluxes, (iii) the rigidity cut-off (or geomagnetic transmission function) and grammage at the counter location, (iv) the atmosphere response to incoming TOA cosmic rays (shower development), and (v) the counter response to the various particles/energies in the shower. Count rates from neutron monitors or muon counters are therefore a proxy to solar activity. In this paper, we review all ingredients, discuss how their uncertainties impact count rate calculations, and how they translate into variation/uncertainties on the level of solar modulation $\varphi$ (in the simple Force-Field approximation). The main uncertainty for neutron monitors is related to the yield function. However, many other effects have a significant impact, at the 5-10\% level on $\varphi$ values. We find no clear ranking of the dominant effects, as some depend on the station position and/or the weather and/or the season. An abacus to translate any variation of count rates (for neutron and $\mu$ detectors) to a variation of the solar modulation $\varphi$ is provided.Comment: 28 pages, 16 figures, 9 tables, match accepted version in AdSR (minor corrections, Dorman (1974,2004,2009) reference textbooks added

State of Health Equity Movement, 2011 Update: DRA Project Report No. 11-01

State of Health Equity Movement, 2011 Update Part A: Overview DRA Project Report No. 11-0

Nanobody-Based Probes for Subcellular Protein Identification and Visualization

Understanding how building blocks of life contribute to physiology is greatly aided by protein identification and cellular localization. The two main labeling approaches developed over the past decades are labeling with antibodies such as immunoglobulin G (IgGs) or use of genetically encoded tags such as fluorescent proteins. However, IgGs are large proteins (150 kDa), which limits penetration depth and uncertainty of target position caused by up to ∼25 nm distance of the label created by the chosen targeting approach. Additionally, IgGs cannot be easily recombinantly modulated and engineered as part of fusion proteins because they consist of multiple independent translated chains. In the last decade single domain antigen binding proteins are being explored in bioscience as a tool in revealing molecular identity and localization to overcome limitations by IgGs. These nanobodies have several potential benefits over routine applications. Because of their small size (15 kDa), nanobodies better penetrate during labeling procedures and improve resolution. Moreover, nanobodies cDNA can easily be fused with other cDNA. Multidomain proteins can thus be easily engineered consisting of domains for targeting (nanobodies) and visualization by fluorescence microscopy (fluorescent proteins) or electron microscopy (based on certain enzymes). Additional modules for e.g., purification are also easily added. These nanobody-based probes can be applied in cells for live-cell endogenous protein detection or may be purified prior to use on molecules, cells or tissues. Here, we present the current state of nanobody-based probes and their implementation in microscopy, including pitfalls and potential future opportunities