Optimizing the anaerobic digestion of microalgae in a coupled process

International audienceThis work is devoted to maximizing the production of methane in a bioreactor coupling an anaerobic digester and a culture of micro-algae limited by light. The decision parameter is the dilution rate which is chosen as a control, and we enforce periodic constraints in order to repeat the same operation every day. The system is gathered into a three-dimensional system taking into account a day-night model of the light in the culture of micro-algae. Applying Pontryagin maximum principle, the necessary conditions on optimal trajectories indicate that the control consists of bang and/or singular arcs. We provide numerical simulations by both direct and indirect methods, which show the link between the light model and the structure of optimal solutions

Integrating microalgae production with anaerobic digestion: a biorefinery approach

This is the peer reviewed version of the following article: [Uggetti, E. , Sialve, B. , Trably, E. and Steyer, J. (2014), Integrating microalgae production with anaerobic digestion: a biorefinery approach. Biofuels, Bioprod. Bioref, 8: 516-529. doi:10.1002/bbb.1469], which has been published in final form at https://doi.org/10.1002/bbb.1469. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-ArchivingIn the energy and chemical sectors, alternative production chains should be considered in order to simultaneously reduce the dependence on oil and mitigate climate change. Biomass is probably the only viable alternative to fossil resources for production of liquid transportation fuels and chemicals since, besides fossils, it is one of the only available sources of carbon-rich material on Earth. Over recent years, interest in microalgae biomass has grown in both fundamental and applied research fields. The biorefinery concept includes different technologies able to convert biomass into added-value chemicals, products (food and feed) and biofuels (biodiesel, bioethanol, biohydrogen). As in oil refinery, a biorefinery aims at producing multiple products, maximizing the value derived from differences in biomass components, including microalgae. This paper provides an overview of the various microalgae-derived products, focusing on anaerobic digestion for conversion of microalgal biomass into methane. Special attention is paid to the range of possible inputs for anaerobic digestion (microalgal biomass and microalgal residue after lipid extraction) and the outputs resulting from the process (e.g. biogas and digestate). The strong interest in microalgae anaerobic digestion lies in its ability to mineralize microalgae containing organic nitrogen and phosphorus, resulting in a flux of ammonium and phosphate that can then be used as substrate for growing microalgae or that can be further processed to produce fertilizers. At present, anaerobic digestion outputs can provide nutrients, CO2 and water to cultivate microalgae, which in turn, are used as substrate for methane and fertilizer generation.Peer ReviewedPostprint (author's final draft

Analysis of a periodic optimal control problem connected to microalgae anaerobic digestion

International audienceIn this work, we study the coupling of a culture of microalgae limited by light and an anaerobic digester in a two-tank bioreactor. The model for the reactor combines a periodic day-night light for the culture of microalgae and a classical chemostat model for the digester. We first prove the existence and attraction of periodic solutions of this problem for a 1 day period. Then, we study the optimal control problem of optimizing the production of methane in the digester during a certain timeframe, the control on the system being the dilution rate (the input flow of microalgae in the digester). We apply Pontryagin's Maximum Principle in order to characterize optimal controls, including the computation of singular controls. We present numerical simulations by direct and indirect methods for different light models and compare the optimal 1-day periodic solution to the optimal strategy over larger timeframes. Finally, we also investigate the dependence of the optimal cost with respect to the volume ratio of the two tanks

Chapter Valorization of Microalgae and Energy Resources

Microalgae biotechnology has grown very rapidly in the last few decades due to the multiple applications that these microorganisms have from pharmaceuticals and cosmetics to foods/feeds and biofuels. One of the main challenges in expanding this industry is to enlarge the single use of the biomass produced in addition to reducing the high biomass production cost of the current technologies. To overcome this bottleneck, the development of microalgae-based biorefineries has been proposed. The issue is to obtain as many bioproducts as possible from the cultivated biomass, including biofuels. Consequently, biodiesel production (from the lipid fraction), bioethanol (from carbohydrate fraction), and biogas or bio-oil (from the whole biomass) have been posited. In this book chapter, we review the current state of the art in the production of sustainable biofuels from microalgae and analyze the potential of microalgae to contribute to the biofuel sector

Valorization of Microalgae and Energy Resources

Microalgae biotechnology has grown very rapidly in the last few decades due to the multiple applications that these microorganisms have from pharmaceuticals and cosmetics to foods/feeds and biofuels. One of the main challenges in expanding this industry is to enlarge the single use of the biomass produced in addition to reducing the high biomass production cost of the current technologies. To overcome this bottleneck, the development of microalgae-based biorefineries has been proposed. The issue is to obtain as many bioproducts as possible from the cultivated biomass, including biofuels. Consequently, biodiesel production (from the lipid fraction), bioethanol (from carbohydrate fraction), and biogas or bio-oil (from the whole biomass) have been posited. In this book chapter, we review the current state of the art in the production of sustainable biofuels from microalgae and analyze the potential of microalgae to contribute to the biofuel sector

Prospects of microalgal biodiesel production in Pakistan – a review

Biodiesel is an alternative, renewable, biodegradable and environmentally friendly fuel for transportation, with properties like petroleum-derived diesel, and can be used directly in a compression ignition engine without any modifications. The world's fossil fuel and crude oil reserves are going to dry up in the next few decades, but, contrariwise, an attractive, high quality, readily available and economically extractable oil from microalgae is a substitute feedstock to produce alternative biodiesel fuel for the transportation sector in the future. Microalgae have a higher biomass productivity (tons/hectare/year) and lipid yield (kg/kg of algal biomass) as compared to vegetable oil crops. To overcome the problem of energy deficiency in developing countries, like Pakistan, and boost their economic growth, alternative fuels are proving very important for environment-friendly and sustainable development, especially in the last few decades. Different research studies on microalgae cultivation, characterization of microalgae oil (lipids), and evaluations of its socio-economic feasibility to produce renewable biodiesel have been conducted in the past in Pakistan for its future prospects. This review paper includes the overall summary and compilation of the microalgae research conducted in Pakistan on biodiesel production and includes the algal biodiesel production cost analysis. The studies showed promising results for harnessing microalgae and using its lipids to produce biodiesel with favourable properties that were comparable to the conventional diesel in Pakistan. The information related to the microalgae research will help stakeholders and governmental organisations working in the renewable energy sector to consider its cultivation on a large scale, using waste water as a feedstock to produce biodiesel to meet the target set by the Government of Pakistan of using 10% blended biodiesel by the year 2025 in Pakistan

Treatment of wastewater using response surface methodology: a brief review

Response surface methodology (RSM) is widely applied to gathering knowledge on the interactions among parameters that require optimization during the treatment of wastewater. It can be used to optimize parameters during the process of treating wastewater, e.g., landfill leachate. The experimental design methods are useful to evaluate the parameters involved in a treatment with the minimum number of experiments. This will reduce the need for reagents and materials for experiments, which finally causes both time and expense to be increased. Anaerobic digestion of wastewater technologies escalated depending on the design configuration of the reactor. Several important parameters are taken into consideration in designing an anaerobic reactor such as operating conditions, seed sludge, wastewater composition and mixing. To construct a highly efficient degradation system, it is necessary to optimize such effective parameters. As a result, the advanced statistical design is used for process characterization, optimization and modelling. In this paper, the fundamentals of RSM and its application in the anaerobic treatment of wastewater was discussed in brief. The various works done in an anaerobic reactor using RSM for prediction and optimization are given

Municipal wastewater treatment with pond technology : historical review and future outlook

Facing an unprecedented population growth, it is difficult to overstress the assets for wastewater treatment of waste stabilization ponds (WSPs), i.e. high removal efficiency, simplicity, and low cost, which have been recognized by numerous scientists and operators. However, stricter discharge standards, changes in wastewater compounds, high emissions of greenhouse gases, and elevated land prices have led to their replacements in many places. This review aims at delivering a comprehensive overview of the historical development and current state of WSPs, and providing further insights to deal with their limitations in the future. The 21st century is witnessing changes in the way of approaching conventional problems in pond technology, in which WSPs should no longer be considered as a low treatment technology. Advanced models and technologies have been integrated for better design, control, and management. The roles of algae, which have been crucial as solar-powered aeration, will continue being a key solution. Yet, the separation of suspended algae to avoid deterioration of the effluent remains a major challenge in WSPs while in the case of high algal rate pond, further research is needed to maximize algal growth yield, select proper strains, and optimize harvesting methods to put algal biomass production in practice. Significant gaps need to be filled in understanding mechanisms of greenhouse gas emission, climate change mitigation, pond ecosystem services, and the fate and toxicity of emerging contaminants. From these insights, adaptation strategies are developed to deal with new opportunities and future challenges

New frontiers from removal to recycling of nitrogen and phosphorus from wastewater in the Circular Economy

[EN] Nutrient recovery technologies are rapidly expanding due to the need for the appropriate recycling of key elements from waste resources in order to move towards a truly sustainable modern society based on the Circular Economy. Nutrient recycling is a promising strategy for reducing the depletion of non-renewable resources and the environmental impact linked to their extraction and manufacture. However, nutrient recovery technologies are not yet fully mature, as further research is needed to optimize process efficiency and enhance their commercial applicability. This paper reviews state-of-the-art of nutrient recovery, focusing on frontier technological advances and economic and environmental innovation perspectives. The potentials and limitations of different technologies are discussed, covering systems based on membranes, photosynthesis, crystallization and other physical and biological nutrient recovery systems (e.g. incineration, composting, stripping and absorption and enhanced biological phosphorus recovery).Robles Martínez, Á.; Aguado García, D.; Barat, R.; Borrás Falomir, L.; Bouzas Blanco, A.; Bautista-Giménez, J.; Martí Ortega, N.... (2020). New frontiers from removal to recycling of nitrogen and phosphorus from wastewater in the Circular Economy. Bioresource Technology. 300:1-18. https://doi.org/10.1016/j.biortech.2019.122673S11830

Coupling photosynthetic biogas upgrading with the astaxanthin production using Neochloris sp

The feasibility of coupling photosynthetic biogas upgrading with astaxanthin production by Neochloris sp. using real centrate as a feedstock was herein evaluated. To maximize astaxanthin production, this study focused on optimizing the cultivation conditions such as light intensity, culture medium, and composition of biogas. A light intensity of 300 μmol m-2 s-1 showed the highest specific growth rate (0.34 d-1) and total suspended solid of biomass (0.33±0.03 g L-1) of microalgae compared to other light intensity (0, 100, 500, 800 μmol m-2 s-1). When the headspace in the bottles was filled with air, mineral salt medium showed higher growth (0.78±0.007 g L-1) than other medium conditions (10% diluted centrate from domestic wastewater, 10% diluted centrate adjusted initial pH of 7, and 10% diluted centrate with activated sludge aliquot). On the other hand, when biogas was injected into the headspace in the bottles, 10% diluted centrate showed a higher specific growth rate (0.58-1.1 d-1) and TSS (2.57±0.92~6.98±0.029 g L-1) because pH control by CO2 dissolution prevented growth inhibition by free ammonia. In terms of biogas upgrading, CO2 of the headspace in the bottles was removed by 82.8-99.8% and decarbonization of biogas was achieved. Interestingly, biogas containing 600 ppm H2S revealed a two-fold higher biomass yield than biogas without H2S. When 5000 ppm of H2S, growth inhibition occurred, but addition of activated sludge aliquot was effective to remove H2S by sulfur oxidizing bacteria, and microalgae grew (4.36±0.057 g L-1). Thus, this study indicated that Neochloris sp. could validated the proof of concept with biogas containing 600 ppm H2S. Comparison of the maximum biomass yield with different dilution centrate under a headspace of biogas with 600 ppm H2S showed no significant differences. Astaxanthin yield were 0.072% of dry biomass (MSM), 0.024% (10% diluted centrate), 0.028% (50% diluted centrate), and 0.177% (raw centrate (100% centrate)) on day 10. From these results, 100% centrate can be utilized for Neochloris sp. cultivation. Thus, photosynthetic biogas upgrading using Neochloris sp. can support the valorization of residual effluents such as digestate and biogas in the form of high added value pigments and biomass.Departamento de Ingeniería Química y Tecnología del Medio AmbienteMáster en Ingeniería Ambienta