16,072 research outputs found
Efficient Bayesian-based Multi-View Deconvolution
Light sheet fluorescence microscopy is able to image large specimen with high
resolution by imaging the sam- ples from multiple angles. Multi-view
deconvolution can significantly improve the resolution and contrast of the
images, but its application has been limited due to the large size of the
datasets. Here we present a Bayesian- based derivation of multi-view
deconvolution that drastically improves the convergence time and provide a fast
implementation utilizing graphics hardware.Comment: 48 pages, 20 figures, 1 table, under review at Nature Method
OpenSPIM - an open access platform for light sheet microscopy
Light sheet microscopy promises to revolutionize developmental biology by
enabling live in toto imaging of entire embryos with minimal phototoxicity. We
present detailed instructions for building a compact and customizable Selective
Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware
and software platform is shared with the scientific community through a public
website, thereby making light sheet microscopy accessible for widespread use
and optimization to various applications.Comment: 7 pages, 3 figures, 6 supplementary videos, submitted to Nature
Methods, associated public website http://openspim.or
Quantum-inspired computational imaging
Computational imaging combines measurement and computational methods with the aim of forming images even when the measurement conditions are weak, few in number, or highly indirect. The recent surge in quantum-inspired imaging sensors, together with a new wave of algorithms allowing on-chip, scalable and robust data processing, has induced an increase of activity with notable results in the domain of low-light flux imaging and sensing. We provide an overview of the major challenges encountered in low-illumination (e.g., ultrafast) imaging and how these problems have recently been addressed for imaging applications in extreme conditions. These methods provide examples of the future imaging solutions to be developed, for which the best results are expected to arise from an efficient codesign of the sensors and data analysis tools.Y.A. acknowledges support from the UK Royal Academy of Engineering under the Research Fellowship Scheme (RF201617/16/31). S.McL. acknowledges financial support from the UK Engineering and Physical Sciences Research Council (grant EP/J015180/1). V.G. acknowledges support from the U.S. Defense Advanced Research Projects Agency (DARPA) InPho program through U.S. Army Research Office award W911NF-10-1-0404, the U.S. DARPA REVEAL program through contract HR0011-16-C-0030, and U.S. National Science Foundation through grants 1161413 and 1422034. A.H. acknowledges support from U.S. Army Research Office award W911NF-15-1-0479, U.S. Department of the Air Force grant FA8650-15-D-1845, and U.S. Department of Energy National Nuclear Security Administration grant DE-NA0002534. D.F. acknowledges financial support from the UK Engineering and Physical Sciences Research Council (grants EP/M006514/1 and EP/M01326X/1). (RF201617/16/31 - UK Royal Academy of Engineering; EP/J015180/1 - UK Engineering and Physical Sciences Research Council; EP/M006514/1 - UK Engineering and Physical Sciences Research Council; EP/M01326X/1 - UK Engineering and Physical Sciences Research Council; W911NF-10-1-0404 - U.S. Defense Advanced Research Projects Agency (DARPA) InPho program through U.S. Army Research Office; HR0011-16-C-0030 - U.S. DARPA REVEAL program; 1161413 - U.S. National Science Foundation; 1422034 - U.S. National Science Foundation; W911NF-15-1-0479 - U.S. Army Research Office; FA8650-15-D-1845 - U.S. Department of the Air Force; DE-NA0002534 - U.S. Department of Energy National Nuclear Security Administration)Accepted manuscrip
Optical techniques for 3D surface reconstruction in computer-assisted laparoscopic surgery
One of the main challenges for computer-assisted surgery (CAS) is to determine the intra-opera- tive morphology and motion of soft-tissues. This information is prerequisite to the registration of multi-modal patient-specific data for enhancing the surgeon’s navigation capabilites by observ- ing beyond exposed tissue surfaces and for providing intelligent control of robotic-assisted in- struments. In minimally invasive surgery (MIS), optical techniques are an increasingly attractive approach for in vivo 3D reconstruction of the soft-tissue surface geometry. This paper reviews the state-of-the-art methods for optical intra-operative 3D reconstruction in laparoscopic surgery and discusses the technical challenges and future perspectives towards clinical translation. With the recent paradigm shift of surgical practice towards MIS and new developments in 3D opti- cal imaging, this is a timely discussion about technologies that could facilitate complex CAS procedures in dynamic and deformable anatomical regions
Data Fusion of Objects Using Techniques Such as Laser Scanning, Structured Light and Photogrammetry for Cultural Heritage Applications
In this paper we present a semi-automatic 2D-3D local registration pipeline
capable of coloring 3D models obtained from 3D scanners by using uncalibrated
images. The proposed pipeline exploits the Structure from Motion (SfM)
technique in order to reconstruct a sparse representation of the 3D object and
obtain the camera parameters from image feature matches. We then coarsely
register the reconstructed 3D model to the scanned one through the Scale
Iterative Closest Point (SICP) algorithm. SICP provides the global scale,
rotation and translation parameters, using minimal manual user intervention. In
the final processing stage, a local registration refinement algorithm optimizes
the color projection of the aligned photos on the 3D object removing the
blurring/ghosting artefacts introduced due to small inaccuracies during the
registration. The proposed pipeline is capable of handling real world cases
with a range of characteristics from objects with low level geometric features
to complex ones
Multitarget Tracking in Nonoverlapping Cameras Using a Reference Set
Tracking multiple targets in nonoverlapping cameras are challenging since the observations of the same targets are often separated by time and space. There might be significant appearance change of a target across camera views caused by variations in illumination conditions, poses, and camera imaging characteristics. Consequently, the same target may appear very different in two cameras. Therefore, associating tracks in different camera views directly based on their appearance similarity is difficult and prone to error. In most previous methods, the appearance similarity is computed either using color histograms or based on pretrained brightness transfer function that maps color between cameras. In this paper, a novel reference set based appearance model is proposed to improve multitarget tracking in a network of nonoverlapping cameras. Contrary to previous work, a reference set is constructed for a pair of cameras, containing subjects appearing in both camera views. For track association, instead of directly comparing the appearance of two targets in different camera views, they are compared indirectly via the reference set. Besides global color histograms, texture and shape features are extracted at different locations of a target, and AdaBoost is used to learn the discriminative power of each feature. The effectiveness of the proposed method over the state of the art on two challenging real-world multicamera video data sets is demonstrated by thorough experiments
Phase-Retrieved Tomography enables imaging of a Tumor Spheroid in Mesoscopy Regime
Optical tomographic imaging of biological specimen bases its reliability on
the combination of both accurate experimental measures and advanced
computational techniques. In general, due to high scattering and absorption in
most of the tissues, multi view geometries are required to reduce diffuse halo
and blurring in the reconstructions. Scanning processes are used to acquire the
data but they inevitably introduces perturbation, negating the assumption of
aligned measures. Here we propose an innovative, registration free, imaging
protocol implemented to image a human tumor spheroid at mesoscopic regime. The
technique relies on the calculation of autocorrelation sinogram and object
autocorrelation, finalizing the tomographic reconstruction via a three
dimensional Gerchberg Saxton algorithm that retrieves the missing phase
information. Our method is conceptually simple and focuses on single image
acquisition, regardless of the specimen position in the camera plane. We
demonstrate increased deep resolution abilities, not achievable with the
current approaches, rendering the data alignment process obsolete.Comment: 21 pages, 5 figure
- …