Light sheet microscopy promises to revolutionize developmental biology by
enabling live in toto imaging of entire embryos with minimal phototoxicity. We
present detailed instructions for building a compact and customizable Selective
Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware
and software platform is shared with the scientific community through a public
website, thereby making light sheet microscopy accessible for widespread use
and optimization to various applications.Comment: 7 pages, 3 figures, 6 supplementary videos, submitted to Nature
Methods, associated public website http://openspim.or
