11,429 research outputs found
Towards in vivo g-ratio mapping using MRI: unifying myelin and diffusion imaging
The g-ratio, quantifying the comparative thickness of the myelin sheath
encasing an axon, is a geometrical invariant that has high functional relevance
because of its importance in determining neuronal conduction velocity. Advances
in MRI data acquisition and signal modelling have put in vivo mapping of the
g-ratio, across the entire white matter, within our reach. This capacity would
greatly increase our knowledge of the nervous system: how it functions, and how
it is impacted by disease. This is the second review on the topic of g-ratio
mapping using MRI. As such, it summarizes the most recent developments in the
field, while also providing methodological background pertinent to aggregate
g-ratio weighted mapping, and discussing pitfalls associated with these
approaches. Using simulations based on recently published data, this review
demonstrates the relevance of the calibration step for three myelin-markers
(macromolecular tissue volume, myelin water fraction, and bound pool fraction).
It highlights the need to estimate both the slope and offset of the
relationship between these MRI-based markers and the true myelin volume
fraction if we are really to achieve the goal of precise, high sensitivity
g-ratio mapping in vivo. Other challenges discussed in this review further
evidence the need for gold standard measurements of human brain tissue from ex
vivo histology. We conclude that the quest to find the most appropriate MRI
biomarkers to enable in vivo g-ratio mapping is ongoing, with the potential of
many novel techniques yet to be investigated.Comment: Will be published as a review article in Journal of Neuroscience
Methods as parf of the Special Issue with Hu Cheng and Vince Calhoun as Guest
Editor
Advances in diffusion MRI acquisition and processing in the Human Connectome Project
The Human Connectome Project (HCP) is a collaborative 5-year effort to map human brain connections and their variability in healthy adults. A consortium of HCP investigators will study a population of 1200 healthy adults using multiple imaging modalities, along with extensive behavioral and genetic data. In this overview, we focus on diffusion MRI (dMRI) and the structural connectivity aspect of the project. We present recent advances in acquisition and processing that allow us to obtain very high-quality in-vivo MRI data, whilst enabling scanning of a very large number of subjects. These advances result from 2 years of intensive efforts in optimising many aspects of data acquisition and processing during the piloting phase of the project. The data quality and methods described here are representative of the datasets and processing pipelines that will be made freely available to the community at quarterly intervals, beginning in 2013
Fuzzy Fibers: Uncertainty in dMRI Tractography
Fiber tracking based on diffusion weighted Magnetic Resonance Imaging (dMRI)
allows for noninvasive reconstruction of fiber bundles in the human brain. In
this chapter, we discuss sources of error and uncertainty in this technique,
and review strategies that afford a more reliable interpretation of the
results. This includes methods for computing and rendering probabilistic
tractograms, which estimate precision in the face of measurement noise and
artifacts. However, we also address aspects that have received less attention
so far, such as model selection, partial voluming, and the impact of
parameters, both in preprocessing and in fiber tracking itself. We conclude by
giving impulses for future research
Beyond backscattering: Optical neuroimaging by BRAD
Optical coherence tomography (OCT) is a powerful technology for rapid
volumetric imaging in biomedicine. The bright field imaging approach of
conventional OCT systems is based on the detection of directly backscattered
light, thereby waiving the wealth of information contained in the angular
scattering distribution. Here we demonstrate that the unique features of
few-mode fibers (FMF) enable simultaneous bright and dark field (BRAD) imaging
for OCT. As backscattered light is picked up by the different modes of a FMF
depending upon the angular scattering pattern, we obtain access to the
directional scattering signatures of different tissues by decoupling
illumination and detection paths. We exploit the distinct modal propagation
properties of the FMF in concert with the long coherence lengths provided by
modern wavelength-swept lasers to achieve multiplexing of the different modal
responses into a combined OCT tomogram. We demonstrate BRAD sensing for
distinguishing differently sized microparticles and showcase the performance of
BRAD-OCT imaging with enhanced contrast for ex vivo tumorous tissue in
glioblastoma and neuritic plaques in Alzheimer's disease
Evaluating the accuracy of diffusion MRI models in white matter
Models of diffusion MRI within a voxel are useful for making inferences about
the properties of the tissue and inferring fiber orientation distribution used
by tractography algorithms. A useful model must fit the data accurately.
However, evaluations of model-accuracy of some of the models that are commonly
used in analyzing human white matter have not been published before. Here, we
evaluate model-accuracy of the two main classes of diffusion MRI models. The
diffusion tensor model (DTM) summarizes diffusion as a 3-dimensional Gaussian
distribution. Sparse fascicle models (SFM) summarize the signal as a linear sum
of signals originating from a collection of fascicles oriented in different
directions. We use cross-validation to assess model-accuracy at different
gradient amplitudes (b-values) throughout the white matter. Specifically, we
fit each model to all the white matter voxels in one data set and then use the
model to predict a second, independent data set. This is the first evaluation
of model-accuracy of these models. In most of the white matter the DTM predicts
the data more accurately than test-retest reliability; SFM model-accuracy is
higher than test-retest reliability and also higher than the DTM, particularly
for measurements with (a) a b-value above 1000 in locations containing fiber
crossings, and (b) in the regions of the brain surrounding the optic
radiations. The SFM also has better parameter-validity: it more accurately
estimates the fiber orientation distribution function (fODF) in each voxel,
which is useful for fiber tracking
- …