Characterization of LOCOS and oxidized mesa isolation in deep-sub micrometer SOI NMOS processes

Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1995.Includes bibliographical references (leaves 76-77).by Jeffrey Wade Thomas.M.S

Novice teachers\u27 experiences of community service-learning

This study focuses on beginning teachers\u27 experiences with a currently popular curriculum strategy in the US: community service-learning. To determine the personal and contextual factors influencing novice teachers\u27 experiences, we surveyed over 300 early career teachers and interviewed 30 of the larger sample. The study provides evidence that some beginning teachers are willing to implement strategies they learned in their teacher education programs, and can do so successfully, in spite of being busy and unsupported. Results indicate that specific preparation features and school characteristics may play a large role in whether novice teachers implement service-learning activities in their classrooms

Proteomic Analysis and Identification of Cellular Interactors of the Giant Ubiquitin Ligase HERC2

HERC2 is a large E3 ubiquitin ligase with multiple structural domains that has been implicated in an array of cellular processes. Mutations in HERC2 are linked to developmental delays and impairment caused by nervous system dysfunction, such as Angelman Syndrome and autism-spectrum disorders. However, HERC2 cellular activity and regulation remain poorly understood. We used a broad proteomic approach to survey the landscape of cellular proteins that interact with HERC2. We identified nearly 300 potential interactors, a subset of which we validated binding to HERC2. The potential HERC2 interactors included the eukaryotic translation initiation factor 3 complex, the intracellular transport COPI coatomer complex, the glycogen regulator phosphorylase kinase, beta-catenin, PI3 kinase, and proteins involved in fatty acid transport and iron homeostasis. Through a complex bioinformatic analysis of potential interactors, we linked HERC2 to cellular processes including intracellular protein trafficking and transport, metabolism of cellular energy, and protein translation. Given its size, multidomain structure, and association with various cellular activities, HERC2 may function as a scaffold to integrate protein complexes and bridge critical cellular pathways. This work provides a significant resource with which to interrogate HERC2 function more deeply and evaluate its contributions to mechanisms governing cellular homeostasis and disease

Distal femoral extension osteotomy and patellar tendon advancement or shortening in ambulatory children with cerebral palsy: A modified Delphi consensus study and literature review

PURPOSE In children with cerebral palsy, flexion deformities of the knee can be treated with a distal femoral extension osteotomy combined with either patellar tendon advancement or patellar tendon shortening. The purpose of this study was to establish a consensus through expert orthopedic opinion, using a modified Delphi process to describe the surgical indications for distal femoral extension osteotomy and patellar tendon advancement/patellar tendon shortening. A literature review was also conducted to summarize the recent literature on distal femoral extension osteotomy and patellar tendon shortening/patellar tendon advancement. METHOD A group of 16 pediatric orthopedic surgeons, with more than 10 years of experience in the surgical management of children with cerebral palsy, was established. The group used a 5-level Likert-type scale to record agreement or disagreement with statements regarding distal femoral extension osteotomy and patellar tendon advancement/patellar tendon shortening. Consensus for the surgical indications for distal femoral extension osteotomy and patellar tendon advancement/patellar tendon shortening was achieved through a modified Delphi process. The literature review, summarized studies of clinical outcomes of distal femoral extension osteotomy/patellar tendon shortening/patellar tendon advancement, published between 2008 and 2022. RESULTS There was a high level of agreement with consensus for 31 out of 44 (70%) statements on distal femoral extension osteotomy. Agreement was lower for patellar tendon advancement/patellar tendon shortening with consensus reached for 8 of 21 (38%) of statements. The literature review included 25 studies which revealed variation in operative technique for distal femoral extension osteotomy, patellar tendon advancement, and patellar tendon shortening. Distal femoral extension osteotomy and patellar tendon advancement/patellar tendon shortening were generally effective in correcting knee flexion deformities and extensor lag, but there was marked variation in outcomes and complication rates. CONCLUSION The results from this study will provide guidelines for surgeons who care for children with cerebral palsy and point to unresolved questions for further research. LEVEL OF EVIDENCE level V

Peculiar Type II Supernovae from Blue Supergiants

The vast majority of Type II supernovae (SNe) are produced by red supergiants (RSGs), but SN 1987A revealed that blue supergiants (BSGs) can produce members of this class as well, albeit with some peculiar properties. This best studied event revolutionized our understanding of SNe, and linking it to the bulk of Type II events is essential. We present here optical photometry and spectroscopy gathered for SN 2000cb, which is clearly not a standard Type II SN and yet is not a SN 1987A analog. The light curve of SN 2000cb is reminiscent of that of SN 1987A in shape, with a slow rise to a late optical peak, but on substantially different time scales. Spectroscopically, SN 2000cb resembles a normal SN II but with ejecta velocities that far exceed those measured for SN 1987A or normal SNe II, above 18000 km/s for H-alpha at early times. The red colours, high velocities, late photometric peak, and our modeling of this object all point toward a scenario involving the high-energy explosion of a small-radius star, most likely a BSG, producing 0.1 solar masses of Ni-56. Adding a similar object to the sample, SN 2005ci, we derive a rate of about 2% of the core-collapse rate for this loosely defined class of BSG explosions.Comment: Accepted to MNRAS on March 14, 201

Fourteen Months of Observations of the Possible Super-Chandrasekhar Mass Type Ia Supernova 2009dc

In this paper, we present and analyse optical photometry and spectra of the extremely luminous and slowly evolving Type Ia supernova (SN Ia) 2009dc, and offer evidence that it is a super-Chandrasekhar mass (SC) SN Ia and thus had a SC white dwarf (WD) progenitor. Optical spectra of SN 2007if, a similar object, are also shown. SN 2009dc had one of the most slowly evolving light curves ever observed for a SN Ia, with a rise time of ~23 days and Delta m_15(B) = 0.72 mag. We calculate a lower limit to the peak bolometric luminosity of ~2.4e43 erg/s, though the actual value is likely almost 40% larger. Optical spectra of SN 2009dc and SN 2007if obtained near maximum brightness exhibit strong C II features (indicative of a significant amount of unburned material), and the post-maximum spectra are dominated by iron-group elements. All of our spectra of SN 2009dc and SN 2007if also show low expansion velocities. However, we see no strong evidence in SN 2009dc for a velocity "plateau" near maximum light like the one seen in SN 2007if (Scalzo et al. 2010). The high luminosity and low expansion velocities of SN 2009dc lead us to derive a possible WD progenitor mass of more than 2 M_Sun and a Ni-56 mass of about 1.4-1.7 M_Sun. We propose that the host galaxy of SN 2009dc underwent a gravitational interaction with a neighboring galaxy in the relatively recent past. This may have led to a sudden burst of star formation which could have produced the SC WD progenitor of SN 2009dc and likely turned the neighboring galaxy into a "post-starburst galaxy." No published model seems to match the extreme values observed in SN 2009dc, but simulations do show that such massive progenitors can exist (likely as a result of the merger of two WDs) and can possibly explode as SC SNe Ia.Comment: 30 pages, 16 figures, 8 tables, re-submitted to MNRA

A Genetic Screen Identifies FAN1, a Fanconi Anemia-Associated Nuclease Necessary for DNA Interstrand Crosslink Repair

The Fanconi anemia (FA) pathway is responsible for interstrand crosslink repair. At the heart of this pathway is the FANCI-FAND2 (ID) complex, which, upon ubiquitination by the FA core complex, travels to sites of damage to coordinate repair that includes nucleolytic modification of the DNA urrounding the lesion and translesion synthesis. How the ID complex regulates these events is unknown. Here we describe a shRNA screen that led to the identification of two nucleases necessary for crosslink repair, FAN1 (KIAA1018) and EXDL2. FAN1 colocalizes at sites of DNA damage with the ID complex in a manner dependent on FAN1’s ubiquitin-binding domain (UBZ), the ID complex, and monoubiquitination of FANCD2. FAN1 possesses intrinsic 50 -30 exonuclease activity and endonuclease activity that cleaves nicked and branched structures. We propose that FAN1 is a repair nuclease that is recruited to sites of crosslink damage in part through binding the ubiquitinated ID complex through its UBZ domain