Ecología y condición física del ciervo rojo en la Península Ibérica: implicaciones para la gestión

Doutoramento em BiologiaThe red deer (Cervus elaphus) is currently one of the most widespread and abundant wild ungulates in the Iberian Peninsula and is extremely important both ecologically, as a key species for the functioning of the ecosystems, and economically, as a major game species. In Iberia, red deer populations are subjected to different management systems that may affect the physical condition of the individuals, with further consequences for population dynamics. Studies investigating the effects of management practices and environmental conditions on the performance of red deer are still rare regarding Mediterranean ecosystems. Much of the knowledge concerning the ecology of red deer and the impact of management on its physical condition is based on studies conducted in northern and central regions of Europe, where climatological features and management practices differ from those observed in the Mediterranean areas of Iberia. Studies on a biogeographical scale can provide important insights into the relationships between species and a particular environment and contribute to the development of more targeted and appropriate management practices. The optimisation of sampling procedures and the fine-tuning of pre-existing analytical techniques are also fundamental to a more cost-effective monitoring and, therefore, are of enormous value to wildlife managers. In this context, the main aims of this thesis were: 1) to optimise the procedures used to assess the physical condition of red deer; and 2) to identify relevant management and environmental factors affecting the nutritional condition and stress physiology of red deer in the Mediterranean ecosystems of Iberia, as well as any potential interactions between those factors. Two studies with a methodological focus, presented in the first part of the thesis, demonstrated that the physical condition of red deer can be evaluated more simply, using more cost- and time-effective procedures than those traditionally used: i) it was shown that only one kidney and its associated fat is enough to assess nutritional condition in red deer; and ii) the feasibility of using near infrared spectroscopy to predict the concentrations of stress hormone metabolites was demonstrated using faeces of red deer for the first time. Subsequently, two large-scale observational studies, conducted in representative red deer populations found in Mediterranean Iberia, highlighted the importance of considering seasonal environmental variations and variables related to hunting management practices to better understand the nutritional and physiological ecology of red deer. High population densities had adverse effects on the nutritional condition of the deer and were associated with increased stress levels in natural populations without supplementary feeding. Massive hunting events involving the use of hounds were also identified as a potential source of chronic stress in red deer. The research presented in this thesis has clear implications regarding the management and monitoring of red deer populations in Mediterranean environments and is intended to help wildlife managers to implement more effective monitoring programmes and sustainable management practices.O veado (Cervus elaphus) é, atualmente, uma das espécies de ungulados silvestres mais abundantes na Península Ibérica. É também uma espécie extremamente importante em termos ecológicos, por ser um elemento chave para o funcionamento dos ecossistemas, e socioeconómicos, por ser uma espécie de caça maior emblemática e altamente valorizada. Na Ibéria, as populações de veados estão sujeitas a diferentes práticas de gestão que podem afetar a condição física dos indivíduos de diferentes formas e, por conseguinte, determinar a sua dinâmica. Apesar da importância desta espécie, os estudos sobre os efeitos da gestão cinegética e das condições ambientais nos indicadores da sua condição física são ainda escassos nos ecossistemas mediterrânicos. Grande parte do conhecimento sobre a ecologia do veado e sobre o impacto da sua gestão cinegética é baseado em estudos realizados em regiões do norte e centro da Europa, onde as características climáticas e os sistemas de gestão diferem daqueles existentes nos ambientes mediterrânicos da Península Ibéria. Os estudos realizados a uma escala biogeográfica podem ser bastante úteis para compreender as relações das espécies com o ambiente, e podem contribuir para o desenvolvimento de práticas de gestão mais específicas e adequadas às necessidades de gestão. A otimização de procedimentos de amostragem e do uso de técnicas analíticas é também essencial para uma monitorização mais eficaz das populações silvestres em termos de tempo e custos associados e, portanto, é uma tema de grande interesse para gestores de fauna selvagem. Neste contexto, os principais objetivos desta tese foram: 1) otimizar os métodos usados para avaliar a condição física do veado; e 2) identificar os fatores de gestão cinegética e ambientais que afetam o estado nutricional e a fisiologia do stress do veado nos ecossistemas mediterrânicos da Ibéria, assim como investigar possíveis interações entre esses fatores. Dois estudos de cariz metodológico, apresentados na primeira parte desta tese, demonstraram que a condição física do veado pode ser avaliada usando procedimentos mais simples e rentáveis do que aqueles normalmente utilizados: i) demonstrou-se que o estado nutricional do veado pode ser avaliado usando apenas um rim e a sua gordura perirrenal; ii) provou-se, pela primeira vez, a viabilidade da espectroscopia no infravermelho próximo para estimar concentrações de metabólitos de hormonas de stress usando fezes de veado. Posteriormente, dois estudos observacionais, realizados em diferentes populações de veados representativas da região mediterrânica ibérica, evidenciaram a importância de considerar as variações ambientais sazonais e as variáveis de gestão cinegética para compreender a ecofisiologia e a ecologia nutricional do veado. Observou-se que as densidades populacionais elevadas afetaram de forma negativa o estado nutricional dos veados e estiveram também associadas a níveis de stress mais elevados em populações naturais sem alimentação suplementar. Para além disto, verificou-se que a realização de eventos de caça massivos com matilhas pode ser um fator de stress crónico nas populações de veados. Os resultados obtidos no âmbito desta tese têm diversas implicações no que diz respeito à monitorização e gestão das populações de veados nos ambientes mediterrânicos. Espera-se que os estudos aqui apresentados ajudem os gestores de fauna silvestre a implementar programas de monitorização populacional mais eficazes e práticas de gestão mais sustentáveis.El ciervo rojo (Cervus elaphus) es actualmente uno de los ungulados silvestres más abundantes de la Península Ibérica. Es una especie con una elevada importancia ecológica, siendo un elemento clave en la dinámica de los ecosistemas mediterráneos, y presenta un gran valor socioeconómico, al ser una especie de caza mayor emblemática y muy apreciada. En el territorio peninsular, las poblaciones de ciervos están sometidas a diferentes sistemas de manejo que pueden modular la condición física de los individuos y, consecuentemente, determinar la dinámica de sus poblaciones. Pese a la relevancia de esta especie, aun son escasos los estudios sobre los efectos de la gestión cinegética y de las condiciones ambientales en los indicadores de su condición en los ecosistemas mediterráneos. La mayor parte del conocimiento sobre la ecología de esta especie y sobre el impacto de su gestión cinegética se basa en estudios realizados en regiones del norte y centro de Europa, donde las características climáticas y sistemas de manejo son diferentes a los presentes en los ambientes mediterráneos de la Península Ibérica. Los estudios realizados a una escala biogeográfica pueden ser muy útiles para comprender las relaciones de las especies con el ambiente, y pueden contribuir al desarrollo de prácticas de manejo más especificas y adecuadas a las necesidades de gestión. La optimización de procedimientos de muestreo y la puesta a punto de técnicas analíticas son también de gran importancia para una monitorización más eficaz de las poblaciones silvestres en términos de tiempo y costes asociados y, por consiguiente, son temas de gran interés para los gestores de la fauna silvestre. En este contexto, los objetivos principales de esta tesis doctoral fueron: 1) optimizar los métodos usados para evaluar la condición física del ciervo; y 2) identificar los factores de manejo y ambientales que afectan el estado nutricional y la fisiología del estrés del ciervo en los ecosistemas de la Iberia mediterránea, así como estudiar las posibles interacciones entre dichos factores. Dos estudios con un enfoque metodológico, presentados en la primera parte de esta tesis, demostraron que la condición física del ciervo se puede valorar usando procedimientos más sencillos y rentables que los usados tradicionalmente: i) se ha demostrado que el estado nutricional del ciervo se puede evaluar usando solamente un riñón y su grasa perirrenal; y ii) se comprobó, por primera vez, la viabilidad de la espectroscopia en el infrarrojo cercano para estimar las concentraciones de metabolitos de hormonas de estrés usando heces de ciervo. Posteriormente, dos estudios observacionales basados en poblaciones de ciervo representativas de la Iberia mediterránea evidenciaron la importancia de considerar las variaciones ambientales estacionales y las variables de manejo cinegético para comprender la ecofisiología y la ecología nutricional del ciervo. Se observó que las densidades poblacionales elevadas afectan de forma negativa al estado nutricional de los ciervos y éstas se asociaron con niveles de estrés más elevados en poblaciones naturales sin suplementación alimentaria. Además, se verificó que los eventos de caza masivos con rehalas pueden representar un factor de estrés crónico en las poblaciones de ciervos. Los resultados obtenidos en esta tesis tienen implicaciones en lo que respecta a la monitorización y gestión de las poblaciones de ciervos en ambientes mediterráneos, y se espera que los estudios aquí presentados ayuden a los gestores de fauna silvestre a implementar programas de monitorización más eficaces y prácticas de gestión más sostenibles

Estudo populacional do veado (Cervus elaphus L.) no nordeste transmontano

Mestrado em Ecologia, Biodiversidade e Gestão de EcossistemasO ressurgimento do veado (Cervus elaphus L.) no Nordeste Transmontano, decorrente dos repovoamentos efectuados na Reserva Regional de Caza “Sierra de la Culebra” (Zamora, Espanha) durante a década de 1970, bem como o progressivo aumento do número de efectivos e expansão geográfica verificados ao longo dos anos na região, têm conduzido a uma crescente preocupação no que se refere à conservação, gestão e aproveitamento cinegético deste recurso natural, visto ser uma espécie de inegável valor ecológico e sócio-económico. A definição de estratégias que visem a manutenção e a gestão sustentada da população de veados na Zona de Caça Nacional da Lombada/Parque Natural de Montesinho (Distrito de Bragança, Portugal) deve passar necessariamente por um conhecimento prévio e continuado da situação populacional e das relações da espécie com o meio em que se insere. Neste sentido, os objectivos deste estudo foram: estimar as densidades de veado na área norte (12.000 ha) da ZCN da Lombada através da aplicação de duas metodologias de observação directa (transectos lineares e pontos fixos); caracterizar a estrutura/composição da população; estudar e analisar os padrões de uso do habitat e actualizar a informação referente à área de distribuição da espécie na região. Os resultados obtidos durante as diferentes fases deste estudo confirmaram um crescimento populacional e um aumento da distribuição espacial da espécie no nordeste português relativamente aos dados conhecidos para a última década. Apesar da baixa precisão de algumas estimativas e da discrepância verificada nos valores de densidade média obtidos nas diferentes fases de amostragem realizadas, poder-se-á afirmar que a densidade média real para a área de estudo deverá aproximar-se da estimativa obtida no Inverno de 2009 mediante a aplicação da amostragem de distâncias (Distance sampling) nos transectos lineares, mais precisamente 3,26 veados/100 ha (IC 95% = 2,27 – 4,70). Relativamente a outros parâmetros populacionais, foi possível determinar, para um conjunto de três períodos, um rácio macho/fêmea médio de 0,74 (IC 95% = 0,64 – 0,84), o qual evidencia uma boa situação geral na relação entre sexos, e uma taxa média de recrutamento de crias de 0,37 (IC 95% = 0,29 – 0,44), valor este que reflecte uma produtividade que se pode considerar entre baixa a moderada, quando comparada com outros valores ao nível europeu. No que diz respeito à expansão geográfica da espécie no Parque Natural de Montesinho, verificou-se um incremento na ordem dos 30% da área de distribuição entre os anos de 2002 e de 2008. Tendo em consideração a composição da paisagem na área da ZCN da Lombada/Parque Natural de Montesinho e a importância relativa dos diferentes tipos de habitat para a espécie, pode dizer-se que a região, em termos globais, reúne as condições necessárias para a manutenção e proliferação do veado. ABSTRACT: The reappearance of red deer (Cervus elaphus L.) in the north-east of Trás-os-Montes, due to deer releases in the “Sierra de la Culebra” Regional Hunting Reserve (Zamora, Spain) during the 70s, as well as the population growth and geographic expansion observed throughout the last years in this region, have lead to an increasing concern in relation to conservation, management and game exploitation of this natural resource, given its undeniable ecological and socio-economic value. The definition of strategies for the conservation and sustainable management of red deer population in Lombada´s National Hunting Reserve/Montesinho Natural Park (Bragança, Portugal) must necessarily include a previous and continuous knowledge about population situation and species relationship with the surrounding environment. The aims of this study were to estimate red deer densities in the northern area (12.000 ha) of Lombada´s National Hunting Zone using two different methods (line transects and vantage points); to characterize population structure and composition; to understand habitat use patterns and to update data about population distribution area in the region. The results obtained during the different stages of this study showed an increase in the red deer population density and in geographic range, when compared to available data referring to last decade. Despite the low precision of some estimates and the divergence in the average density calculated during the different phases of this study, we can affirm that the real average density value for surveyed area must be close to 3.26 deer/100 ha (95% CI = 2.27 – 4.70), which was the value estimated during the winter of 2009 using a line transect sampling scheme. In relation to other red deer population parameters, it was possible to calculate, for three periods, an average sex-ratio (male/female) of 0.74 (95% CI = 0.64 – 0.84), which is near a 1:1 proportion, and an average calf recruitment rate of 0.37 (95% CI = 0.29 – 0.44), a value that reflects a low to moderately productive population, when compared to other values in the European context. In terms of distribution area in the Montesinho Natural Park, it was possible to observe an increase in population geographic range of approximately 30% between 2002 and 2008. Taking into account the landscape characteristics throughout Lombada´s National Hunting Zone/Montesinho Natural Park area and the relative importance of the different habitats for the species, it can be argued that, as a whole, the region provides good conditions for survival, maintenance and proliferation of red deer population

Body composition and grip strength constraints in elite male rink-hockey players of contrasting ethnicity

Rink hockey is a highly specialized and physiological demanding sport with sparse research regarding the game and athletes’ characteristics. A cross-sectional study was developed to characterize the body composition and grip strength of elite male rink hockey players and to establish the relationship between ethnicity on body composition and grip strength. A sample of 100 elite rink-hockey athletes aged 26.59 ± 6.02 participated in the study, comprised of 69 Caucasian male adults aged 27.58 ± 6.44 years and 31 Black African male adults aged 24.39 ± 4.27. Body composition was assessed by anthropometric measurements. Static grip strength was assessed with an adjustable dynamometer. Multiple regression analysis was applied to understand which variables constraints body fat percentage (BF) and grip strength. Body mass showed an average of 76.36 ± 9.18 kg for 175.80 ± 5.87 cm of height and BF% of 10.82 ± 5.07%. Maximal right grip strength was 50.91 ± 6.26 kg and 50.27 ± 6.23 kg for left grip strength. Four predictors accounted for 70.01% of the variance of BF%: abdominal circumference (p < 0.001), right thigh circumference (p < 0.001), right calf circumference (p = 0.001) and ethnicity (p = 0.016). Three predictors accounted for 13.1% of the variance of right grip strength: ethnicity (p = 0.013), chronological age (p = 0.024) and right distal thigh circumference (p = 0.014). Results suggest that elite rink hockey athletes have a specific anthropometric identity, which at the elite level may lead to reduced body fat and greater handgrip strength. Ethnicity seems to predict body fat and grip strength in elite rink hockey athletes.info:eu-repo/semantics/publishedVersio

Agreement between dual x-ray absorptiometers using pencil beam and fan beam: indicators of bone health and whole-body plus appendicular tissue composition in adult athletes

OBJECTIVE: The current study was aimed to examine intra-individual variation on indicators of bone health in addition to whole-body plus appendicular tissue measurements using two concurrent assessments based on pencil beam and fan beam dual energy X-ray absorptiometry (DXA) systems in adult athletes from several sports. METHOD: Thirty-two male participants (27.6±10.1 years) were measured on anthropometry including multifrequency bioelectric impedance and air-displacement plethysmography. Bone mineral content (BMC), bone area, fat and lean soft tissue were derived using pencil beam (Lunar DPX-MD+) and fan beam (Lunar iDXA) absorptiometry. Bone mineral density (BMD) was obtained for the femoral neck, trochanter and triangle of ward. Finally, the right thigh was defined as a region of interest (ROI). Analyses comprised intra-class correlation (ICC), Effect size (d) from mean differences of repeated measurements, coefficient of variation (CV) RESULTS: ICC were >0.900 for all measurements. Intra-individual differences were large for BMC (d=1,312; CV=2,7%), bone area (d=1,761; CV=2,7%), fat tissue (d=1,612; CV=11%) and all indicators of appendicular lean soft tissue (d=1,237-1687; CV=2,0-4,1%). A very large difference (d=4,014; CV=8.4%) was diagnosed for lean soft tissue of the ROI. CONCLUSION: Although differences among concurrent instruments for BMC and bone area, the effect size of mean differences was negligible for BMD. Fat and lean soft tissue derived from DXA should be interpreted as reference values (not criterion) due to equipment-related variation, more apparently in the ROI values.info:eu-repo/semantics/publishedVersio

Body composition, strength static and isokinetic, and bone health: comparative study between active adults and amateur soccer players

The Centro de Investigação do Desporto e Actividade Física (Research Unit for the Study of Sport and Physical Activity, uid/dtp/04213/2019) is being funded by Fundação para a Ciência e a Tecnologia. AOW is supported by São Paulo Research Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) process: 2017/27234-2.Objective: To compare tissue composition, total and regional bone mineral content and bone mineral density, static hand grip and knee joint isokinetic strength between amateur soccer players and Control Group. Methods: Cross-sectional study. Air displacement plethysmography was used to estimate body volume and, in turn, density. Body composition, bone mineral content and bone mineral density were assessed for the whole body and at standardized regions using dual energy X-ray absorptiometry. Static grip strength was assessed with an adjustable dynamometer, and peak torque derived from isokinetic strength dynamometer (concentric muscular knee actions at 60°/s). Magnitude of the differences between groups was examined using d-Cohen. Results: Compared to healthy active adults, soccer players showed larger values of whole body bone mineral content (+651g; d=1.60; p<0.01). In addition, differences between groups were large for whole body bone mineral density (d=1.20 to 1.90; p<0.01): lumbar spine, i.e. L1-L4 (+19.4%), upper limbs (+8.6%) and lower limbs (+16.8%). Soccer players attained larger mean values in strength test given by static hand grip protocol (+5.6kg, d=0.99; p<0.01). Conclusion: Soccer adequately regulates body composition and is associated better bone health parameters (bone mineral content and density at whole-body and at particular sites exposed to mechanical loadings).Objetivo: Comparar a composição de tecidos, o conteúdo mineral ósseo e a densidade mineral óssea totais e por regiões do corpo, a força de preensão manual estática, e força isocinética da articulação do joelho, entre um grupo de jogadores de futebol amadores e um Grupo Controle. Métodos: Estudo transversal utilizando pletismografia de ar deslocado para estimar o volume corporal, para subsequente cálculo da densidade corporal. A composição de tecidos, o conteúdo mineral ósseo e a densidade mineral óssea foram avaliados para o corpo todo e regiões padronizadas através da absorciometria de raios-X de dupla energia. A força de preensão manual estática foi avaliada por um dinamômetro ajustável. Os momentos máximos de força das ações musculares concêntricas para os extensores e flexores do joelho foram avaliados pela dinamometria isocinética (60°/s). Foi calculado o valor d-Cohen para apreciar a magnitude do efeito das diferenças entre grupos. Resultados: Os futebolistas apresentaram níveis superiores de conteúdo mineral ósseo em comparação com os adultos ativos do Grupo Controle (+651g; d=1,60; p<0,01) e obtiveram valores superiores de densidade mineral óssea (d: 1,20 a 1,90; p<0,01) para a coluna lombar, L1-L4 (+19,4%), membros superiores (+8,6%) e membros inferiores (+16,8%). Para a força de preensão (estática) a diferença foi moderada (d=0,99; p<0,01) com valores mais elevados apresentados pelo futebolistas (+5,6kg; d=0,99; p<0,01). Conclusão: A prática de futebol promove uma regulação adequada da composição corporal (tecidos magro e gordo) e ganhos na densidade mineral óssea, mais acentuada em partes do corpo com maior exposição aos impactos mecânicos da atividade motora.info:eu-repo/semantics/publishedVersio

Unraveling the real magnitude of illegal wildlife poisoning to halt cryptic biodiversity loss

Illegal wildlife poisoning is a global threat for biodiversity, yet the magnitude of its impact on ecosystems is largely underestimated as most of poisoning episodes remain undetected. Here, we conducted a large-scale field experiment to better understand the real dimension of the illegal wildlife poisoning in terms of composition and number of species and abundance of impacted individuals, as well as the ecological factors driving it. We used camera traps to monitor simulated poison baits placed in 25 study areas in SW Europe and applied Good–Turing theory to estimate the richness of species of the entire assemblage (observed plus undetected). We recorded 3095 individuals from 39 vertebrate species that consumed 94 % of the baits (N = 590). Yet, using sample completeness to estimate the entire species assemblage yielded a total of 47 species exposed to illegal poisoning. The observed assemblage included different trophic and functional groups (from lizards and snakes to apex species among birds and mammals), as well as a 38 % of threatened and near threatened species (according to Spanish and Portuguese vertebrate red list and UICN list). The size (weight) of the bait outstands as a reliable predictor of the number of species (0–8 species/bait, mean = 2) and individuals (0–99 individuals/bait, mean = 5) susceptible to poisoning. The habitat where the bait was placed modulated the abundance of individuals affected (greater in open than in closed habitats). Type of bait and habitat drove the compositional variation of species. Our approach enables uncover entire species assemblages prone to illegal poisoning and their ecological drivers associated, advancing the understanding of the impact of wildlife poisoning in ecosystemsThis work was supported by the project TOXICO funding by “BBVA FOUNDATION GRANTS TO SCIENTIFIC RESEARCH TEAMS, CALL 2018”. WWF Spain and SEO/BirdLife provided access to the ANTíDOTO database of wildlife poisoning in Spain. The Ohio Wesleyan University supported with logistical material (5 camera-traps). JVLB was supported by the Spanish Ministry of Economy, Industry and Competitiveness (RYC-2015-18932; CGL2017-87528-R AEI/FEDER EU). JVLB and PMT were supported by a GRUPIN research grant from the Regional Government of Asturias (AYUD/2021/51314). In Portugal, this study was financed through the Sentinelas project funded by Fundo Ambiental – Minist´erio do Ambiente e da Aç˜ ao Clim´ atica. We are grateful to Instituto da Conservaçao ˜ da Natureza e das Florestas (ICNF), Principado de Asturias, Junta de Castilla y Leon, ´ Xunta de Galicia, Gobierno de Cantabria, Comunidad de Madrid, Junta de Andalucía, Picos de Europa National Park, Sierra de Guadarrama National Park, Cabaneros ˜ National Park and Monfragüe National Park for granted permission

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost