Articulação de Interesses Entre Setor Privado e Poder Público: o Movimento Empresarial Espírito Santo em Ação e o Governo do Estado do Espírito Santo

O presente trabalho insere-se na temática referente à articulação de interesses envolvendo o setor empresarial e o poder público. A proposta é identificar as bases de atuação do Movimento Empresarial Espírito Santo em Ação visando interferir no processo decisório no estado do Espírito Santo. Embora haja uma série de estudos sobre a temática da representação de interesses no Brasil, o enfoque proposto busca trazer novos elementos à discussão tal como, o contexto histórico político-econômico. O Espírito Santo em Ação é uma entidade de representação empresarial, com uma estrutura organizacional fora dos moldes corporativista brasileiro, formada por membros pessoas físicas e empresas de diferentes setores econômicos. A análise permitiu concluir que a principal frente de atuação do Espírito Santo em Ação não foi no processo de produção legislativa, mas sim nos dispositivos que lhe permitiram estabelecer parcerias com o Executivo. Notadamente, a entidade teve ampla participação nos processos de elaboração de planos de desenvolvimentos setoriais e globais do governo Paulo Hartung, participando, portanto, da etapa de formulação das políticas governamentais. No Espírito Santo, o arranjo institucional pertinente à relação entre o Executivo e o Legislativo é fortemente marcado por características do ultrapresidencialismo estadual, no qual o Executivo se sobrepõe ao Legislativo no processo decisório. A análise permitiu observar que o arranjo institucional afeta a leitura que os atores fazem do sistema o que, por sua vez, orienta as articulações e a atuação política do Espírito Santo em Ação. Por fim, vale salientar a importância do contexto histórico político-econômico, tanto no surgimento quanto na proposta de atuação do Espírito Santo em Ação. Palavras chaves: Espírito Santo em Ação, representação de interesses, Espírito Sant

Impact of brain biopsy on management of nonneoplastic brain disease

Introduction: Diagnostic yield of brain biopsy in neoplastic brain disease is high and its clinical impact is well established. In nonneoplastic brain disease with negative conventional investigation, decision to undergo invasive procedures is difficult due to its inherent risk and known lower diagnostic yield. Research question: What is the clinical impact of brain biopsy results on management of nonneoplastic brain disease ? Material and methods: A multidisciplinary team retrospectively reviewed and included all nonneoplastic brain disease cases submitted to biopsy between 2009 and 2019, in a tertiary hospital in Lisbon. Baseline characteristics were registered, including immunosuppression status, diagnostic workup, and treatment prior to biopsy. Diagnostic yield, clinical impact and in-hospital complication rates were assessed. Results: Sixty-four patients were included, 20 (31.3%) of them immunosuppressed (15 HIV ​+ ​patients). Thirty-five (67.7%) were previously treated with steroids or antiinfectious agents, with higher percentage (93.3%) in the immunosuppressed group. Biopsy results were diagnostic in 46 (71.9%) cases. More frequent diagnosis was infectious in 20 (31.2%), neoplastic in 12 (18.8%) and inflammatory diseases in 8 (12.5%). Brain biopsy resulted on impact on patient's clinical management in 56 (87.5%), of which 37(57.8%) were submitted to treatment change. In-hospital complications were registered in 4 (6.6%) patients. Discussion and conclusion: Brain biopsy had clinical impact, including a change in treatment, in most patients studied, and may be considered a useful diagnostic option in nonneoplastic brain disease. However, associated complication rate is not negligible, and previous thorough workup, patient selection and risk-benefit assessment are important.info:eu-repo/semantics/publishedVersio

Synthetic biology approaches to engineer saccharomyces cerevisiae towards the industrial production of valuable polyphenolic compounds

Polyphenols are plant secondary metabolites with diverse biological and potential therapeutic activities such as antioxidant, anti-inflammatory and anticancer, among others. However, their extraction from the native plants is not enough to satisfy the increasing demand for this type of compounds. The development of microbial cell factories to effectively produce polyphenols may represent the most attractive solution to overcome this limitation and produce high amounts of these bioactive molecules. With the advances in the synthetic biology field, the development of efficient microbial cell factories has become easier, largely due to the development of the molecular biology techniques and by the identification of novel isoenzymes in plants or simpler organisms to construct the heterologous pathways. Furthermore, efforts have been made to make the process more profitable through improvements in the host chassis. In this review, advances in the production of polyphenols by genetically engineered Saccharomyces cerevisiae as well as by synthetic biology and metabolic engineering approaches to improve the production of these compounds at industrial settings are discussed.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/BIO/04469/2020 unit and BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the European Regional Development Fund (ERDF) under the scope of Norte2020 – North Portugal Regional Program. In addition, this research has been carried out at the Biomass and Bioenergy Research Infrastructure (BBRI)–LISBOA-010145-FEDER-022059, supported by Operational Program for Competitiveness and Internationalization (PORTUGAL2020), the Lisbon Portugal Regional Operational Program (Lisboa2020), and Norte2020 under the Portugal 2020 Partnership Agreement, through the ERDF. JR is recipient of a fellowship supported by a doctoral advanced training (SFRH/BD/138325/2018) funded by FCT.info:eu-repo/semantics/publishedVersio

Do fisher associations really represent their members’ needs and opinions? The case study of the octopus fishery in the Algarve (south Portugal)

Fishers’ participation in the fishery management decision-making process is generally low, particularly in small-scale fisheries (SSF). Within the overarching goal of improving fisheries governance, fishers’ participation is crucial. Yet, how can fishers participate in the decision-making processes which affect their actions, and to what extent do fisher associations represent their interests? These questions were tackled by means of an empirical case study in the “Tertúlia do Polvo” project, focusing on the octopus SSF in the Algarve region (south Portugal), where the octopus fishery is managed top-down with sporadic participation of fishers. During the study (2014 and 2015), seven participatory workshops (tertúlias) were held, involving fisher associations, management authorities and researchers, to propose and discuss management measures for the fishery. Also, a face-to-face questionnaire survey (121 valid replies) was undertaken with local fishers to gauge their opinions about the management measures proposed during the workshops. Results show a strong agreement between the outcomes achieved during the workshops and the main concerns and possible solutions identified by fishers. Taking into consideration the difficulty in structuring and assuring a transparent and effective participation of fishers in the management of their activity, the results obtained are promising. In this study, the use of a participatory process (restricted to a small group of stakeholders) combined with consultation (targeting a sample of the fisher population) allowed the validation of the overall results obtained. Such a methodological approach can be tried in other fishing communities to implement efficient and effective collaborative management, contributing to improved fisheries governance.FCT: SFRH/BPD/116307/2016. PROMAR: 31-03-01-FEP-200.info:eu-repo/semantics/publishedVersio

Deep brain stimulation of the anterior nucleus of the thalamus in drug-resistant epilepsy in the MORE multicenter patient registry

Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Background and objectives: The efficacy of deep brain stimulation of the anterior nucleus of the thalamus (ANT DBS) in patients with drug-resistant epilepsy (DRE) was demonstrated in the double-blind Stimulation of the Anterior Nucleus of the Thalamus for Epilepsy randomized controlled trial. The Medtronic Registry for Epilepsy (MORE) aims to understand the safety and longer-term effectiveness of ANT DBS therapy in routine clinical practice. Methods: MORE is an observational registry collecting prospective and retrospective clinical data. Participants were at least 18 years old, with focal DRE recruited across 25 centers from 13 countries. They were followed for at least 2 years in terms of seizure frequency (SF), responder rate (RR), health-related quality of life (Quality of Life in Epilepsy Inventory 31), depression, and safety outcomes. Results: Of the 191 patients recruited, 170 (mean [SD] age of 35.6 [10.7] years, 43% female) were implanted with DBS therapy and met all eligibility criteria. At baseline, 38% of patients reported cognitive impairment. The median monthly SF decreased by 33.1% from 15.8 at baseline to 8.8 at 2 years (p 10 implantations) had 42.8% reduction in median monthly SF by 2 years in comparison with 25.8% in low-volume center. In patients with cognitive impairment, the reduction in median monthly SF was 26.0% by 2 years compared with 36.1% in patients without cognitive impairment. The most frequently reported adverse events were changes (e.g., increased frequency/severity) in seizure (16%), memory impairment (patient-reported complaint, 15%), depressive mood (patient-reported complaint, 13%), and epilepsy (12%). One definite sudden unexpected death in epilepsy case was reported. Discussion: The MORE registry supports the effectiveness and safety of ANT DBS therapy in a real-world setting in the 2 years following implantation. Classification of evidence: This study provides Class IV evidence that ANT DBS reduces the frequency of seizures in patients with drug-resistant focal epilepsy.The MORE registry was sponsored and funded by Medtronic, plc.info:eu-repo/semantics/publishedVersio

Tracing the Distribution of European Lactase Persistence Genotypes Along the Americas

In adulthood, the ability to digest lactose, the main sugar present in milk of mammals, is a phenotype (lactase persistence) observed in historically herder populations, mainly Northern Europeans, Eastern Africans, and Middle Eastern nomads. As the –13910∗T allele in the MCM6 gene is the most well-characterized allele responsible for the lactase persistence phenotype, the –13910C > T (rs4988235) polymorphism is commonly evaluated in lactase persistence studies. Lactase non-persistent adults may develop symptoms of lactose intolerance when consuming dairy products. In the Americas, there is no evidence of the consumption of these products until the arrival of Europeans. However, several American countries’ dietary guidelines recommend consuming dairy for adequate human nutrition and health promotion. Considering the extensive use of dairy and the complex ancestry of Pan-American admixed populations, we studied the distribution of –13910C > T lactase persistence genotypes and its flanking haplotypes of European origin in 7,428 individuals from several Pan-American admixed populations. We found that the –13910∗T allele frequency in Pan-American admixed populations is directly correlated with allele frequency of the European sources. Moreover, we did not observe any overrepresentation of European haplotypes in the –13910C > T flanking region, suggesting no selective pressure after admixture in the Americas. Finally, considering the dominant effect of the –13910∗T allele, our results indicate that Pan-American admixed populations are likely to have higher frequency of lactose intolerance, suggesting that general dietary guidelines deserve further evaluation across the continent

Do fisher associations really represent their members’ needs and opinions? The case study of the octopus fishery in the Algarve (south Portugal)

29 pages, 3 figures, 4 tables.Fishers’ participation in the fishery management decision-making process is generally low, particularly in small-scale fisheries (SSF). Within the overarching goal of improving fisheries governance, fishers’ participation is crucial. Yet, how can fishers participate in the decision-making processes which affect their actions, and to what extent do fisher associations represent their interests? These questions were tackled by means of an empirical case study in the “Tertúlia do Polvo” project, focusing on the octopus SSF in the Algarve region (south Portugal), where the octopus fishery is managed top-down with sporadic participation of fishers. During the study (2014 and 2015), seven participatory workshops (tertúlias) were held, involving fisher associations, management authorities and researchers, to propose and discuss management measures for the fishery. Also, a face-to-face questionnaire survey (121 valid replies) was undertaken with local fishers to gauge their opinions about the management measures proposed during the workshops. Results show a strong agreement between the outcomes achieved during the workshops and the main concerns and possible solutions identified by fishers. Taking into consideration the difficulty in structuring and assuring a transparent and effective participation of fishers in the management of their activity, the results obtained are promising. In this study, the use of a participatory process (restricted to a small group of stakeholders) combined with consultation (targeting a sample of the fisher population) allowed the validation of the overall results obtained. Such a methodological approach can be tried in other fishing communities to implement efficient and effective collaborative management, contributing to improved fisheries governance.Peer reviewe

Reasons for Living Inventory for Young Adults: Psychometric Properties Among Portuguese Sample

A main protective factor against suicide in young adults is their reasons for living; therefore, suicide risk screening should consider these reasons. However, few psychometric instruments assess reasons for living, and none have been adapted for young adults in Portugal. Thus, we assess the psychometric characteristics of the Reasons for Living Inventory for Young Adults-II (RFL-YA-II) in participants (n = 936; Mage = 21.77; SD = 2.88) from Portugal. Participants answered measures concerning suicidal ideation, depression, hopelessness, and positive and negative affect. The results of an exploratory factorial analysis replicated the original 4-factor model of the RFL-YA-II, and a confirmatory factorial analysis indicated satisfactory indices. In terms of reliability and convergent, discriminant, and concurrent validity, our results are consistent with previous research. Moreover, our results indicate that the RFL-YA-II is a valid and reliable instrument to study the protective factors against suicidal behavior in Portuguese young adults, and should thus be integrated into preventive strategies.FCT -Fundacao para a Ciencia e a Tecnologia -as part the project CIP/UAL [UID/PSI/04345/2019]info:eu-repo/semantics/publishedVersio