Metabolomics in the Analysis of Inflammatory Diseases

Most infections and traumatic injuries are cleared or repaired relatively rapidly and metabolic homoeostasis is soon restored. However, there is a broad range of inflammatory diseases which involve chronic activation of the immune system and, as a result, chronic persistent inflammation. We have been studying the metabolic consequences of chronic inflammatory diseases with the aim of identifying metabolic fingerprints which may provide clues about why the localised tissue disease persists

The Arecibo Legacy Fast ALFA Survey: VIII. HI Source Catalog of the Anti-Virgo Region at dec = +25 deg

We present a fourth catalog of HI sources from the Arecibo Legacy Fast ALFA (ALFALFA) Survey. We report 541 detections over 136 deg2, within the region of the sky having 22h < R.A. < 03h and 24 deg < Dec. < 26 deg . This complements a previous catalog in the region 26 deg < Dec. < 28 deg (Saintonge et al. 2008). We present here the detections falling into three classes: (a) extragalactic sources with S/N > 6.5, where the reliability of the catalog is better than 95%; (b) extragalactic sources 5.0 < S/N < 6.5 and a previously measured optical redshift that corroborates our detection; or (c) High Velocity Clouds (HVCs), or subcomponents of such clouds, in the periphery of the Milky Way. Of the 541 objects presented here, 90 are associated with High Velocity Clouds, while the remaining 451 are identified as extragalactic objects. Optical counterparts have been matched with all but one of the extragalactic objects.Comment: 26 pages, 5 figures, 1 table, accepted for publication in Astrophysical Journal Supplement Serie

Variation of bending rigidity with material density: bilayer silica with nanoscale holes

Two dimensional (2D) materials are a young class of materials that is foreseen to play an important role as building blocks in a range of applications, e.g. flexible electronics. For such applications, mechanical properties such as the bending rigidity κ are important. Only a few published measurements of the bending rigidity are available for 2D materials. Nearly unexplored is the question of how the 2D material density influences the bending rigidity. Here, we present helium atom scattering measurements on a “holey” bilayer silica with a density of 1.4 mg m−2, corresponding to 1.7 monolayers coverage. We find a bending rigidity of 6.6 ± 0.3 meV, which is lower than previously published measurements for a complete 2D film, where a value of 8.8 ± 0.5 meV was obtained. The decrease of bending rigidity with lower density is in agreement with theoretical predictions.publishedVersio

Temperature-Dependent Bending Rigidity of AB-Stacked Bilayer Graphene

The change in bending rigidity with temperature κ(T) for 2D materials is highly debated: theoretical works predict both increase and decrease. Here we present measurements of κ(T), for a 2D material: AB-stacked bilayer graphene. We obtain κ(T) from phonon dispersion curves measured with helium atom scattering in the temperature range 320–400 K. We find that the bending rigidity increases with temperature. Assuming a linear dependence over the measured temperature region we obtain κ(T)=[(1.3 ± 0.1) + (0.006 ± 0.001)T/K] eV by fitting the data. We discuss this result in the context of existing predictions and room temperature measurements.publishedVersio

The Arecibo Legacy Fast ALFA Survey: VI. Second HI Source Catalog of the Virgo Cluster Region

We present the third installment of HI sources extracted from the Arecibo Legacy Fast ALFA extragalactic survey. This dataset continues the work of the Virgo ALFALFA catalog. The catalogs and spectra published here consist of data obtained during the 2005 and 2006 observing sessions of the survey. The catalog consists of 578 HI detections within the range 11h 36m < R.A.(J2000) < 13h 52m and +08 deg < Dec.(J2000) < +12 deg, and cz_sun < 18000 km/s. The catalog entries are identified with optical counterparts where possible through the examination of digitized optical images. The catalog detections can be classified into three categories: (a) detections of high reliability with S/N > 6.5; (b) high velocity clouds in the Milky Way or its periphery; and (c) signals of lower S/N which coincide spatially with an optical object and known redshift. 75% of the sources are newly published HI detections. Of particular note is a complex of HI clouds projected between M87 and M49 that do not coincide with any optical counterparts. Candidate objects without optical counterparts are few. The median redshift for this sample is 6500 km/s and the cz distribution exhibits the local large scale structure consisting of Virgo and the background void and the A1367-Coma supercluster regime at cz_sun ~7000 km/s. Position corrections for telescope pointing errors are applied to the dataset by comparing ALFALFA continuum centroid with those cataloged in the NRAO VLA Sky Survey. The uncorrected positional accuracy averages 27 arcsec ~(21 arcsec ~median) for all sources with S/N > 6.5 and is of order ~21 arcsec ~(16 arcsec ~median) for signals with S/N > 12. Uncertainties in distances toward the Virgo cluster can affect the calculated HI mass distribution.Comment: 25 pages, 1 Table, 8 figures, Accepted by the Astronomical Journa

Glutaredoxin GRXS17 associates with the cytosolic iron-sulfur cluster assembly pathway

Cytosolic monothiol glutaredoxins (GRXs) are required in iron-sulfur (Fe-S) cluster delivery and iron sensing in yeast and mammals. In plants, it is unclear whether they have similar functions. Arabidopsis (Arabidopsis thaliana) has a sole class II cytosolic monothiol GRX encoded by GRXS17. Here, we used tandem affinity purification to establish that Arabidopsis GRXS17 associates with most known cytosolic Fe-S assembly (CIA) components. Similar to mutant plants with defective CIA components, grxs17 loss-of-function mutants showed some degree of hypersensitivity to DNA damage and elevated expression of DNA damage marker genes. We also found that several putative Fe-S client proteins directly bind to GRXS17, such as XANTHINE DEHYDROGENASE1 (XDH1), involved in the purine salvage pathway, and CYTOSOLIC THIOURIDYLASE SUBUNIT1 and CYTOSOLIC THIOURIDYLASE SUBUNIT2, both essential for the 2-thiolation step of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification of tRNAs. Correspondingly, profiling of the grxs17-1 mutant pointed to a perturbed flux through the purine degradation pathway and revealed that it phenocopied mutants in the elongator subunit ELO3, essential for the mcm5 tRNA modification step, although we did not find XDH1 activity or tRNA thiolation to be markedly reduced in the grxs17-1 mutant. Taken together, our data suggest that plant cytosolic monothiol GRXs associate with the CIA complex, as in other eukaryotes, and contribute to, but are not essential for, the correct functioning of client Fe-S proteins in unchallenged conditions

Scale-free correlations in the dynamics of a small (N ~ 10000) cortical network

The advent of novel opto-genetics technology allows the recording of brain activity with a resolution never seen before. The characterisation of these very large data sets offers new challenges as well as unique theory-testing opportunities. Here we discuss whether the spatial and temporal correlation of the collective activity of thousands of neurons are tangled as predicted by the theory of critical phenomena. The analysis shows that both, the correlation length $\xi$ and the correlation time $\tau$ scale as predicted as a function of the system size. With some peculiarities that we discuss, the analysis uncovers new evidence consistent with the view that the large scale brain cortical dynamics corresponds to critical phenomena.Comment: 8 pages, 6 figure

Are conductance plateaus independent events in atomic point contact measurements ? A statistical approach

Room temperature conductance-elongation curves of gold atomic wires are measured using a Scanning Tunnelling Microscope Break Junction technique. Landauer's conductance plateaus are individually identified and statistically analysed. Both the probabilities to observe, and the lengths of the two last plateaus (at conductance values close to 2e2/h and 4e2/h) are studied. All results converge to show that the occurrence of these two conductance plateaus on a conductance-elongation curve are statistically independent events.Comment: 8 pages, 5 figures. Accepted for publication in Nanotechnolog