Potential of Probing the Lunar Regolith using Rover-Mounted Ground Penetrating Radar: Moses Lake Dune Field Analog Study

Probing radars have been widely recognized by the science community to be an efficient tool to explore lunar subsurface providing a unique capability to address several scientific and operational issues. A wideband (200 to 1200 MHz) Ground Penetrating Radar (GPR) mounted on a surface rover can provide high vertical resolution and probing depth from few tens of centimeters to few tens of meters depending on the sounding frequency and the ground conductivity. This in term can provide a better understand regolith thickness, elemental iron concentration (including ilmenite), volatile presence, structural anomalies and fracturing. All those objectives are of important significance for understanding the local geology and potential sustainable resources for future landing sites in particular exploring the thickness, structural heterogeneity and potential volatiles presence in the lunar regolith. While the operation and data collection of GPR is a straightforward case for most terrestrial surveys, it is a challenging task for remote planetary study especially on robotic platforms due to the complexity of remote operation in rough terrains and the data collection constrains imposed by the mechanical motion of the rover and limitation in data transfer. Nevertheless, Rover mounted GPR can be of great support to perform systematic subsurface surveys for a given landing site as it can provide scientific and operational support in exploring subsurface resources and sample collections which can increase the efficiency of the EVA activities for potential human crews as part of the NASA Constellation Program. In this study we attempt to explore the operational challenges and their impact on the EVA scientific return for operating a rover mounted GPR in support of potential human activity on the moon. In this first field study, we mainly focused on the ability of GPR to support subsurface sample collection and explore shallow subsurface volatiles

EMSO-ANTARES (Western Ligurian Sea) a unique observatory for sea science and particle astrophysics

Peer Reviewe

High-level secretion of recombinant monomeric murine and human single-chain Fv antibodies from Drosophila S2 cells

Single-chain variable fragment (scFvs) antibodies are small polypeptides (∼26 kD) containing the heavy (VH) and light (VL) immunoglobulin domains of a parent antibody connected by a flexible linker. In addition to being frequently used in diagnostics and therapy for an increasing number of human diseases, scFvs are important tools for structural biology as crystallization chaperones. Although scFvs can be expressed in many different organisms, the expression level of an scFv strongly depends on its particular amino acid sequence. We report here a system allowing for easy and efficient cloning of (i) scFvs selected by phage display and (ii) individual heavy and light chain sequences from hybridoma cDNA into expression plasmids engineered for secretion of the recombinant fragment produced in Drosophila S2 cells. We validated the method by producing five scFvs derived from human and murine parent antibodies directed against various antigens. The production yields varied between 5 and 12 mg monomeric scFv per liter of supernatant, indicating a relative independence on the individual sequences. The recombinant scFvs bound their cognate antigen with high affinity, comparable with the parent antibodies. The suitability of the produced recombinant fragments for structural studies was demonstrated by crystallization and structure determination of one of the produced scFvs, derived from a broadly neutralizing antibody against the major glycoprotein E2 of the hepatitis C virus. Structural comparison with the Protein Data Bank revealed the typical spatial organization of VH and VL domains, further validating the here-reported expression system

Sickle Cell Trait and Kidney Disease in People of African Ancestry With HIV

Introduction: Sickle cell trait (SCT) has been associated with chronic kidney disease (CKD) in African Americans, although evidence for its impact in Africans and people with HIV is currently lacking. We conducted a cross-sectional study investigating the association between SCT and kidney disease in people of African ancestry with HIV in the UK. Methods: The primary outcome was estimated glomerular filtration rate (eGFR) 50 mg/mmol), and albuminuria (albumin-to-creatinine ratio >3 mg/mmol). Multivariable logistic regression was used to estimate the associations between SCT and kidney disease outcomes. Results: A total of 2895 participants (mean age 48.1 [SD 10.3], 57.2% female) were included, of whom 335 (11.6%) had SCT and 352 (12.2%) had eGFR <60 ml/min per 1.73 m2. After adjusting for demographic, HIV, and kidney risk factors including APOL1 high-risk genotype status, individuals with SCT were more likely to have eGFR <60 ml/min per 1.73 m2 (odds ratio 1.62 [95% CI 1.14–2.32]), eGFR <90 ml/min per 1.73 m2 (1.50 [1.14–1.97]), and albuminuria (1.50 [1.09–2.05]). Stratified by APOL1 status, significant associations between SCT and GFR <60 ml/min per 1.73 m2, eGFR <90 ml/min per 1.73 m2, proteinuria, and albuminuria were observed for those with APOL1 low-risk genotypes. Conclusion: Our results extend previously reported associations between SCT and kidney disease to people with HIV. In people of African ancestry with HIV, these associations were largely restricted to those with APOL1 low-risk genotypes

Genetic Variants of APOL1 Are Major Determinants of Kidney Failure in People of African Ancestry With HIV

INTRODUCTION: Variants of the APOL1 gene are associated with chronic kidney disease (CKD) in people of African ancestry, although evidence for their impact in people with HIV are sparse. METHODS: We conducted a cross-sectional study investigating the association between APOL1 renal risk alleles and kidney disease in people of African ancestry with HIV in the UK. The primary outcome was end-stage kidney disease (ESKD; estimated glomerular filtration rate [eGFR] of 30 mg/mmol), and biopsy-proven HIV-associated nephropathy (HIVAN). Multivariable logistic regression was used to estimate the associations between APOL1 high-risk genotypes (G1/G1, G1/G2, G2/G2) and kidney disease outcomes. RESULTS: A total of 2864 participants (mean age 48.1 [SD 10.3], 57.3% female) were genotyped, of whom, 354 (12.4%) had APOL1 high-risk genotypes, and 99 (3.5%) had ESKD. After adjusting for demographic, HIV, and renal risk factors, individuals with APOL1 high-risk genotypes were at increased odds of ESKD (odds ratio [OR] 10.58, 95% CI 6.22–17.99), renal impairment (OR 5.50, 95% CI 3.81–7.95), albuminuria (OR 3.34, 95% CI 2.00–5.56), and HIVAN (OR 30.16, 95% CI 12.48–72.88). An estimated 49% of ESKD was attributable to APOL1 high-risk genotypes. CONCLUSION: APOL1 high-risk genotypes were strongly associated with kidney disease in people of African ancestry with HIV and accounted for approximately half of ESKD cases in this cohort

Performance evaluation of a combined ADCP- scientific echosounder system.

International audienceEchosounders are widely used to quantify fish behavior, fish stocks, and zooplankton biomass. Acoustic Doppler Current Profilers have also been used to accurately measure currents in all of the world’s major water bodies over the last 30 years. The present work evaluates the performance of a combined echosounder/ADCP system, the Nortek Signature100, for simultaneous biomass assessment and current profile data analysis. Due to its combined current profiling and scientific echosounding capabilities, the system is seeing increased usage in biomass flux applications, particularly in Antarctic krill research. However, capabilities of the system are still being studied and the present work aims to expand characterization of its performance. To that effect, a four month deployment was carried out by the French National Center for Scientific Research (CNRS) in the Mediterranean Sea with an up-looking Signature100 mounted atop the ALBATROSS mooring line. The line was at a total water depth of 2420 m and its top was approximately 370 m below the surface. Data show significant variations in scattering conditions between daytime and nighttime due to diel vertical migration (DVM), often unrelated to horizontal velocity fluctuations, highlighting not only the multiple frequency band capabilities of the system (up to 7 bands), but also the strength of the combined echosounder and current profiling functions. Echoview, a commercial software package for hydroacoustic data processing, was used to further explore the spatial and temporal patterns of the organisms observed in the echosounder data. A semi-automated technique was implemented to efficiently and objectively clean (e.g. remove interference generated by passing ship traffic), classify (e.g. based on relative frequency response or morphology), and characterize the narrow bandwidth and pulse compressed echosounder data by generating outputs that can contribute to the management and monitoring of aquatic resources

Anomalies of noble gases and self-potential associated with fractures and fluid dynamics in a horizontal borehole, Mont Terri Underground Rock Laboratory

Before the excavation of the new gallery Ga08 in the Mont Terri Underground Rock Laboratory (Switzerland), which joined the existing gallery Ga04 in 2008, the end-face of gallery Ga04 was instrumented in 2007 to characterize the evolution of the rock mass with geochemical and geophysical methods. The noble gas content of a 12-m long horizontal borehole evidenced that desaturation processes occurred in the first 2 m, where pre-existing fractures accommodated the stress change during excavation of gallery Ga04 four years before. These first 2 m are associated with the so-called Excavation Damaged Zone (EDZ). As an inflow of pore water was observed in this borehole few weeks after its drilling, continuous self-potential (SP) measurements were performed to characterize its dynamics. After the drilling of new sub-horizontal boreholes in the end-face, strong localized variations of SP occurred. The comparison with the geological features of the rock mass suggests that these dynamic anomalies have to be associated with fluid circulation in pre-existing tectonic fractures that were primarily reactivated by the excavation of the gallery Ga04 and subsequently by the drilling operations

Anomalies of noble gases and self-potential associated with fractures and fluid dynamics in a horizontal borehole, Mont Terri Underground Rock Laboratory

Before the excavation of the new gallery Ga08 in the Mont Terri Underground Rock Laboratory (Switzerland), which joined the existing gallery Ga04 in 2008, the end-face of gallery Ga04 was instrumented in 2007 to characterize the evolution of the rock mass with geochemical and geophysical methods. The noble gas content of a 12-m long horizontal borehole evidenced that desaturation processes occurred in the first 2 m, where pre-existing fractures accommodated the stress change during excavation of gallery Ga04 four years before. These first 2 m are associated with the so-called Excavation Damaged Zone (EDZ). As an inflow of pore water was observed in this borehole few weeks after its drilling, continuous self-potential (SP) measurements were performed to characterize its dynamics. After the drilling of new sub-horizontal boreholes in the end-face, strong localized variations of SP occurred. The comparison with the geological features of the rock mass suggests that these dynamic anomalies have to be associated with fluid circulation in pre-existing tectonic fractures that were primarily reactivated by the excavation of the gallery Ga04 and subsequently by the drilling operations

Glider monitoring of shelf suspended particle dynamics and transport during storm and flooding conditions

International audienceTransfers of particulate matter on continental margins primarily occur during energetic events. As part of the CASCADE (CAscading, Storm, Convection, Advection and Downwelling Events) experiment, a glider equipped with optical sensors was deployed in the coastal area of the Gulf of Lions, NW Mediterranean in March 2011 to assess the spatio-temporal variability of hydrology, suspended particles properties and fluxes during energetic conditions. This deployment complemented a larger observational effort, a part of the MOOSE (Mediterranean Ocean Observing System of the Environment) network, composed of a coastal benthic station, a surface buoy and moorings on the continental slope. This set of observations permitted to measure the impact of three consecutive storms and a flood event across the entire continental shelf. Glider data showed that the sediment resuspension and transport observed at the coastal station during the largest storm (Hs>4 m) was effective down to a water depth of 80 m. The mid-shelf mud belt, located between 40 and 90 m depth, appears as the zone where the along-shelf flux of suspended sediment is maximum. Besides, the across-shelf flux of suspended sediment converges towards the outer limit of the mid-shelf mud belt, where deposition of suspended particles probably occurs and contributes to the nourishment of this area. Hydrological structures, suspended particles transport and properties changed drastically during stormy periods and the following flood event. Prior to the storms, the shelf waters were weakly stratified due in particular to the presence of cold dense water on the inner- and mid-shelf. The storms rapidly swept away this dense water, as well as the resuspended sediments, along the shelf and towards a downstream submarine canyon. The buoyant river plumes that spread along the shelf after the flooding period provoked a restratification of the water column on the inner- and mid-shelf. The analysis of glider's optical data at different wavelengths suggests that the coastal area and the bottom nepheloid layer during the largest storm are primarily composed of coarse particles, probably macroflocs, and that the size of particles decreases further offshore. A similar trend, albeit less contrasted, is observed after the flooding. This work provided a unique synoptic view across the entire shelf of the impact of a typical Mediterranean storm on bottom sediment erosion and particulate fluxes. Repeated glider transects across the south-western part of the Gulf of Lions shelf permitted for the first time to measure continuously the thermo-haline structures, the suspended particles concentrations and size, the current speed, and to estimate the particulate transport before, during and after typical Mediterranean storm events. Glider data complement and compare well with concomitant high frequency time series at fixed stations along the coast and in a downstream submarine canyon