International audienceEchosounders are widely used to quantify fish behavior, fish stocks, and zooplankton biomass. Acoustic Doppler Current Profilers have also been used to accurately measure currents in all of the world’s major water bodies over the last 30 years. The present work evaluates the performance of a combined echosounder/ADCP system, the Nortek Signature100, for simultaneous biomass assessment and current profile data analysis. Due to its combined current profiling and scientific echosounding capabilities, the system is seeing increased usage in biomass flux applications, particularly in Antarctic krill research. However, capabilities of the system are still being studied and the present work aims to expand characterization of its performance. To that effect, a four month deployment was carried out by the French National Center for Scientific Research (CNRS) in the Mediterranean Sea with an up-looking Signature100 mounted atop the ALBATROSS mooring line. The line was at a total water depth of 2420 m and its top was approximately 370 m below the surface. Data show significant variations in scattering conditions between daytime and nighttime due to diel vertical migration (DVM), often unrelated to horizontal velocity fluctuations, highlighting not only the multiple frequency band capabilities of the system (up to 7 bands), but also the strength of the combined echosounder and current profiling functions. Echoview, a commercial software package for hydroacoustic data processing, was used to further explore the spatial and temporal patterns of the organisms observed in the echosounder data. A semi-automated technique was implemented to efficiently and objectively clean (e.g. remove interference generated by passing ship traffic), classify (e.g. based on relative frequency response or morphology), and characterize the narrow bandwidth and pulse compressed echosounder data by generating outputs that can contribute to the management and monitoring of aquatic resources