CARACTERIZACIÓN DEL ACEITE ESENCIAL DE ORÉGANO LISO (Poliomintha longiflora Gray) DE LA LOCALIDAD INFIERNILLO EN EL MUNICIPIO DE HIGUERAS, N.L., MEXICO

EL presente trabajo se realizó en la Facultad de Agronomía de la Universidad Autónoma de Nuevo León y en el Centro de Investigación para los Recursos Naturales (CIReNa) en Salaices López Chihuahua. Se caracterizó el aceite esencial de orégano liso (Poliomintha longiflora Gray) cosechado en el municipio de Higueras, Nuevo León, específicamente en la localidad de Infiernillo en La Sierra de Picachos. Se seleccionaron aleatoriamente cinco sitios de muestreo, delimitándose un área de 10 m X 3 m (30 m2), en cada uno de ellos. Se tomaron datos de la ubicación geográfica, la altura de planta, y la cobertura foliar. Posteriormente el material vegetativo fue colocado en una cámara de secado por 72 horas a una temperatura de 65°C. Se separaron las hojas de los tallos. Se evaluó la producción por hectárea de la biomasa verde y seca. Para los análisis bromatológicos se molió una muestra representativa de 15 gramos de hojas secas, y se determinó: materia seca, cenizas, proteína cruda, fibra cruda, extracto etéreo y extracto libre de Nitrógeno (1). En el laboratorio del CIReNa se realizó la extracción del aceite esencial, utilizando el método de arrastre con vapor de agua, y con el aceite recolectado se preparó la muestra para caracterizarlo en el Cromatógrafo de Gases Masas. Así mismo, se evaluó el rendimiento del aceite. La caracterización total de los compuestos presentes en el aceite de orégano fueron determinados en la Universidad Autónoma de Chihuahua. Los resultados mostraron un avance en el estudio de la especie conocida como orégano liso (Poliomintha longiflora), ya que hasta la fecha no existían reportes sobrela composición de los aceites esenciales.Palabras clave: orégano, aceite esencial, análisis quimicooregano, essential oil, chemical analyse

Fluctuations in phenolic content, ascorbic acid and total carotenoids and antioxidant activity of fruit beverages during storage

Stability of the total phenolic content, ascorbic acid, total carotenoids and antioxidant activity in eight fruit beverages was analyzed. The influence of storage temperature (4, 8 and 11 °C) during the product shelf-life (20 days) was evaluated. Pomegranate Juice presented the highest values for antioxidant activity by DPPH• assay (552.93 ± 6.00 GAE μg mL−1), total carotenoids (3.18 ± 0.11 βCE μgmL−1), and total phenolic content (3967.07 ± 2.47 GAE μg mL−1); while Splash Blend recorded the highest levels of ascorbic acid (607.39 ± 2.13 AAE μg mL−1). The antioxidant capacity was stable at 4 and 8 °C for the first 8 days of storage; while carotenoids and ascorbic acid were slightly degraded through the storage time, possibly due to oxidation and/or reactions with other compounds. The results suggest that the observed variation during testing could be related to storage conditions of the final product

Effectiveness of an intervention for improving drug prescription in primary care patients with multimorbidity and polypharmacy:Study protocol of a cluster randomized clinical trial (Multi-PAP project)

This study was funded by the Fondo de Investigaciones Sanitarias ISCIII (Grant Numbers PI15/00276, PI15/00572, PI15/00996), REDISSEC (Project Numbers RD12/0001/0012, RD16/0001/0005), and the European Regional Development Fund ("A way to build Europe").Background: Multimorbidity is associated with negative effects both on people's health and on healthcare systems. A key problem linked to multimorbidity is polypharmacy, which in turn is associated with increased risk of partly preventable adverse effects, including mortality. The Ariadne principles describe a model of care based on a thorough assessment of diseases, treatments (and potential interactions), clinical status, context and preferences of patients with multimorbidity, with the aim of prioritizing and sharing realistic treatment goals that guide an individualized management. The aim of this study is to evaluate the effectiveness of a complex intervention that implements the Ariadne principles in a population of young-old patients with multimorbidity and polypharmacy. The intervention seeks to improve the appropriateness of prescribing in primary care (PC), as measured by the medication appropriateness index (MAI) score at 6 and 12months, as compared with usual care. Methods/Design: Design:pragmatic cluster randomized clinical trial. Unit of randomization: family physician (FP). Unit of analysis: patient. Scope: PC health centres in three autonomous communities: Aragon, Madrid, and Andalusia (Spain). Population: patients aged 65-74years with multimorbidity (≥3 chronic diseases) and polypharmacy (≥5 drugs prescribed in ≥3months). Sample size: n=400 (200 per study arm). Intervention: complex intervention based on the implementation of the Ariadne principles with two components: (1) FP training and (2) FP-patient interview. Outcomes: MAI score, health services use, quality of life (Euroqol 5D-5L), pharmacotherapy and adherence to treatment (Morisky-Green, Haynes-Sackett), and clinical and socio-demographic variables. Statistical analysis: primary outcome is the difference in MAI score between T0 and T1 and corresponding 95% confidence interval. Adjustment for confounding factors will be performed by multilevel analysis. All analyses will be carried out in accordance with the intention-to-treat principle. Discussion: It is essential to provide evidence concerning interventions on PC patients with polypharmacy and multimorbidity, conducted in the context of routine clinical practice, and involving young-old patients with significant potential for preventing negative health outcomes. Trial registration: Clinicaltrials.gov, NCT02866799Publisher PDFPeer reviewe

Bacterias acidolácticas como promotoras de la digestibilidad in vitro e in vivo / [por] Juana Aranda Ruiz.

Tesis (Maestría en Ciencias en Producción Animal) UANLUANLhttp://www.uanl.mx

Novel Flaviviruses Detected in Different Species of Mosquitoes in Spain

We report the characterization of three novel flaviviruses isolated in Spain. Marisma Mosquito virus, a novel mosquito borne virus, was isolated from Ochlerotatus caspius mosquitoes; Spanish Ochlerotatus flavivirus and Spanish Culex flavivirus, two novel insect flaviviruses, were isolated from Oc. caspius and Culex pipiens, re- spectively. During this investigation, we designed a sensitive RT-nested polymerase chain reaction method that amplifies a 1019bp fragment of the flavivirus NS5 gene and could be directly used in clinical or environmental samples for flavivirus characterization and surveillance. Analysis of the sequence generated from that amplicon contains enough phylogenetic information for proper taxonomic studies. Moreover, the use of this tool allowed the detection of additional flavivirus DNA forms in Culex, Culiseta, and Ochlerotatus mosquitoesPeer reviewe

Influence of extraction solvent on phenolic content and antioxidant capacity level of a commercial food supplement from Moringa oleifera leaves

The objective of this research was to evaluate the effect of extraction solvent on phenolic compounds content (total phenols, total flavonoids) and antioxidant capacity levels (DPPH, ABTS and FRAP) of a commercial food supplement from Moringa oleifera leaves. The content of total phenols, total flavonoids and total non-flavonoids ranged from 55.98 to 226.20 mg ChlAE/g, from 17.63 to 23.46 mg CatE/g and from 38.34 to 202.73 mg CatE/g mg/g, respectively, while condensed tannins were not detected in none of the extracts. Non-flavonoids compounds were the most abundant group of phenolics in all the extracts ranging from 68 to 90% of the total phenols content. Levels of antioxidant capacity ranged from 22.43 to 124.93, 101.03 to 245.25 and 77.06 to 214.17 μmolTE/g in ABTS, DPPH and FRAP, respectively. In addition, EtOH 50% and EtOH 100% extracts showed the highest and lowest content in phenolics content and antioxidant capacity level. Finally, data obtained in the content of phenolics and antioxidant capacity levels of the present study are higher than most of data of scientific literature reported previously by other authors.El objetivo de esta investigación fue evaluar el efecto del solvente de extracción en el contenido de compuestos fenólicos (fenoles totales, flavonoides totales) y los niveles de capacidad antioxidante (DPPH, ABTS y FRAP) de un suplemento comercial de hojas de Moringa oleifera. El contenido de fenoles totales, flavonoides totales, no-flavonoides totales oscilaron de 55.98 a 226.20 mg ChlAE/g, de 17.63 a 23.46 mg CatE/g y de 38.34 a 202.73 mg/g, respectivamente, mientras que no fue detectada la presencia de taninos condensados en ninguno de los extractos. Los compuestos fenólicos noflavonoides fueron el grupo más abundante de compuestos fenólicos en todos los extractos y oscilaron de 68 a 90% del total de los fenoles totales. Los niveles de capacidad oscilaron de 22.43 a 124.93, de 101.03 a 245.25 y de 77.06 a 214.17 μmolTE/g en ABTS, DPPH y FRAP, respectivamente. Además, los extractos con EtOH 50% y EtOH 100% mostraron el contenido más alto y más bajo de fenólicos y niveles de capacidad antioxidante, respectivamente. Finalmente, los datos obtenidos en el contenido de compuestos fenólicos y los niveles de capacidad antioxidante del presente estudio son más altos que la mayoría de los datos reportados previamente en literatura por otros autores