29 research outputs found

    RNAi-mediated suppression of isoprene emission in poplar transiently impacts phenolic metabolism under high temperature and high light intensities: a transcriptomic and metabolomic analysis

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    In plants, isoprene plays a dual role: (a) as thermo-protective agent proposed to prevent degradation of enzymes/membrane structures involved in photosynthesis, and (b) as reactive molecule reducing abiotic oxidative stress. The present work addresses the question whether suppression of isoprene emission interferes with genome wide transcription rates and metabolite fluxes in grey poplar (Populusxcanescens) throughout the growing season. Gene expression and metabolite profiles of isoprene emitting wild type plants and RNAi-mediated non-isoprene emitting poplars were compared by using poplar Affymetrix microarrays and non-targeted FT-ICR-MS (Fourier transform ion cyclotron resonance mass spectrometry). We observed a transcriptional down-regulation of genes encoding enzymes of phenylpropanoid regulatory and biosynthetic pathways, as well as distinct metabolic down-regulation of condensed tannins and anthocyanins, in non-isoprene emitting genotypes during July, when high temperature and light intensities possibly caused transient drought stress, as indicated by stomatal closure. Under these conditions leaves of non-isoprene emitting plants accumulated hydrogen peroxide (H2O2), a signaling molecule in stress response and negative regulator of anthocyanin biosynthesis. The absence of isoprene emission under high temperature and light stress resulted transiently in a new chemo(pheno)type with suppressed production of phenolic compounds. This may compromise inducible defenses and may render non-isoprene emitting poplars more susceptible to environmental stress

    Climate control of terrestrial carbon exchange across biomes and continents

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    Localizing EU Gender Policy: The Diffusion of Gender Mainstreaming across Feminist Movements in Eastern Germany, CES Germany & Europe Working Papers, No. 08.1, June, 2008

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    Gender mainstreaming emerged in the mid-1990s as an innovative and controversial policy tool for reducing gender inequalities. The European Union seeks to propagate the practice of gender mainstreaming both within EU institutions and among member states. Feminist scholars and policy elites discuss and debate gender main-streaming widely, but have yet to consider how local feminist activists, who could play a central role in diffusing gender mainstreaming, understand, interpret and respond to this agenda. This paper examines whether and why local feminist movements in two cities in eastern Germany adopt gender mainstreaming. Consideration of the characteristics of the contexts in which local feminist movements are embedded clarifies the conditions under which social movements rally round new policy paradigms

    Challenging and Reinforcing the Ability/Disability System through Advocacy for Disabled Dogs

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    This paper illuminates how volunteers and animal rescuers who assist dogs from a high-intake public shelter in the Los Angeles metropolitan area simultaneously resist the devaluation of the lives of disabled dogs while sensationalizing and fetishizing disability through their discursive and representational practices. Drawing on observations from three years of ethnographic fieldwork in an animal shelter and with animal rescues, as well as from public media volunteers and rescuers post online, I adopt an intersectional analysis that attends to inequalities of species, ability/disability, and gender in the context of contemporary American capitalism. I show how members of the animal rescue community—who are all women, almost all white, and none of whom identified themselves as disabled—reject the shelter's practice of fast-tracking some types of disabled dogs for shelter killing and assert instead the right of disabled dogs to live. At the same time, rescuers talk about and represent disabled dogs as infantile, remarkable, and in need of saviors to help them. Ultimately, rescuers' representations of disabled dogs work to expand capitalist beliefs of companion animals as lively capital by showing that disabled dogs have value as companions, in large part through an assertion of dogs as family members
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