Reacción en cadena de la polimerasa para la detección de Streptococcus mutans y Streptococcus sobrinus en placa dental de preescolares de Cartagena, Colombia.

Objectives: To detect the presence of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children from Cartagena and correlate it to dental caries precavity stages, applying a standardized PCR-based technique for epidemiological purposes. Methods: Descriptive study using a non-probabilistic sample of 50 children between 3 and 5 years of age, preschoolers from a Caribbean population in Colombia. Criteria for selection were that children should exhibit plaque accumulations on the surface of the cervical margins of the rearmost molars, and placed in one of two study groups: carious lesions and sound surfaces. Dental plaque samples from both groups were subjected to molecular analysis and statistical analysis was applied to determine the difference between the two groups using the frequencies of presence of S. mutans, S. sobrinus or both in the two groups applying Fisher’s exact test for association between the presence of microorganisms and the state of the tooth surface from where the dental plaque was taken. Results: The frequency of S. mutans in carious lesions was 76% and 24% in healthy surfaces. The frequency of S. sobrinus in carious lesions was 81.9% and 18.1% in caries-free surfaces. There was statistical significance between the presence of S. mutans and the presence of caries (p=0.001) and between the presence of S. sobrinus (p=0.02) and the presence of caries. There was no statistical significance between the presence of caries and the simultaneous presence of both microorganisms (p=0.08). Conclusions: The presence of S. mutans and S. sobrinus in dental plaque samples is highly prevalent and associated to non cavitated carious lesions, being the molecular identification of these microorganisms by PCR a sensitive, fast, and easy to use detection method for the mutans group of oral bacteria. Objetivos: Detectar la presencia de Streptococcus mutans y Streptococcus sobrinus en placa dental de niños de Cartagena y relacionarlo con la caries dental en estadíos precavitacionales, estandarizándose la técnica basada en PCR para detectar a nivel epidemiológico la presencia de estos microorganismos. Métodos: Estudio de tipo descriptivo, a partir de una muestra no probabilística de 50 niños entre 3 y 5 años de los hogares infantiles de una población del Caribe colombiano. Como criterios de selección se tuvo en cuenta que los niños presentaran acumulaciones de placa dental sobre la superficie de los márgenes cervicales de los últimos molares. El estudio se dividió en dos grupos: lesiones cariosas y superficies sanas. Las mediciones se realizaron a partir de muestras de placa dental, sometidas a análisis molecular. El análisis estadístico se realizó a partir de las frecuencias de presencia de S. mutans, S. sobrinus o ambos en las lesiones cariosas y superficies sanas, utilizando la prueba exacta de Fisher para la asociación entre la presencia de los microorganismos y el estado de la superficie dental donde se encontró la placa. Resultados: La frecuencia de S. mutans para las lesiones cariosas fue 76% y 24% en superficies sanas. Con respecto a S. sobrinus, la frecuencia en lesiones cariosas fue 81.9% y 18.1% en superficies sin caries. La presencia de caries estuvo asociada significativamente en forma individual con S. mutans (p=0.001) o con S. sobrinus (p=0.02), pero no fue significante con la presencia simultánea de los dos microorganismos (p=0.08). Conclusiones: La presencia de S. mutans y S. sobrinus en muestras de placa bacteriana se considera un factor de asociación con la presencia de lesiones de caries precavitacionales, siendo la identificación molecular por PCR un método sensible, rápido y de fácil uso para la detección de bacterias del grupo mutans

IL18 Gene Variants Influence the Susceptibility to Chagas Disease

Chagas disease is a parasitic disorder caused by the infection with the flagellated protozoan Trypanosoma cruzi. According to the World Health Organization, more than six million people are currently infected in endemic regions. Genetic factors have been proposed to influence predisposition to infection and development of severe clinical phenotypes like chronic Chagas cardiomyopathy (CCC). Interleukin 18 (IL18) encodes a proinflammatory cytokine that has been proposed to be involved in controlling T. cruzi infection. In this study, we analyzed the possible role of six IL18 gene variants (rs5744258, rs360722, rs2043055, rs187238, rs1946518 and rs360719), which cover most of the variation within the locus, in the susceptibility to infection by T. cruzi and/or CCC. In total, 1,171 individuals from a Colombian region endemic for Chagas disease, classified as seronegative (n = 595), seropositive asymptomatic (n = 175) and CCC (n = 401), were genotyped using TaqMan probes. Significant associations with T. cruzi infection were observed when comparing seronegative and seropositive individuals for rs187238 (P = 2.18E-03, OR = 0.77), rs360719 (P = 1.49E-03, OR = 0.76), rs2043055 (P = 2.52E-03, OR = 1.29), and rs1946518 (P = 0.0162, OR = 1.22). However, dependence analyses suggested that the association was mainly driven by the polymorphism rs360719. This variant is located within the promoter region of the IL18 gene, and it has been described that it creates a binding site for the transcription factor OCT-1 affecting IL-18 expression levels. In addition, no evidence of association was observed between any of the analyzed IL18 gene polymorphisms and the development of CCC. In summary, our data suggest that genetic variation within the promoter region of IL18 is directly involved in the susceptibility to infection by T. cruzi, which provides novel insight into disease pathophysiology and adds new perspectives to achieve a more effective disease control.This work is part of the doctoral thesis “Estudio de las bases genéticas de la enfermedad de Chagas” from the Biomedicine PhD program at the Universidad de Granada (Spain).Peer reviewe

Software libre: Una herramienta para apoyar procesos formativos en la educación colombiana

En la sociedad de la información logramos encontrar variedad de herramientas productivas para el desarrollo educativo a través de las TIC’s (Tecnologías de la Información y las Comunicaciones) a costo cero. El fenómeno de tener a disposición software libre se hace relevante cuando los programas o aplicativos que encontramos para apoyar la educación son además de calidad y cuentan con el aporte de profesionales y desarrolladores de software en todo el mundo. Conviene entonces contribuir en el análisis del impacto del software libre en la educación colombiana y mirar la manera como ha incursionado y progresado en las dinámicas de formación en las instituciones y ha generado procesos educativos de gran calidad en los cuales los actores de la educación se encuentran inmerso

Posibles efectos de la escala de Planck en la propagación de neutrinos de muy alta energía

El objetivo del trabajo es comprender de forma analítica qué posibles consecuencias podría tener la violación de la invariancia de Lorentz en la detección de neutrinos de muy alta energía

Manifestaciones orales del síndrome de Maroteaux-Lamy (Mucopolisacaridosis VI)

La mucopolisacaridosis tipo VI, también conocida como síndrome de Maroteaux-Lamy, es un trastorno lisosómico autosómico recesivo, causado por la deficiencia de la enzima arilsulfatasa B, lo que conduce a la acumulación de dermatán sulfato en los tejidos y su excreción urinaria. La deposición de mucopolisacáridos genera un trastorno progresivo que afecta a múltiples órganos y que, a menudo, resulta en la muerte a temprana edad. Esta enfermedad tiene varias manifestaciones orales, entre las que destacan las complicaciones dentales, que pueden ser graves e incluir folículos similares a quistes dentígeros, maloclusiones, defectos condilares e hiperplasia gingival, además de características clínicas como cuello corto, opacidad corneal, macroglosia y agrandamiento del cráneo, dimensión anteroposterior larga y mano en garra. Se presenta el caso de un paciente de 14 meses de edad que acudió a consulta de odontopediatría por episodios de fiebre, bajo peso e hiperplasia gingival severa. El examen físico evidenció facies tosca, cuello corto, pectus excavatus, manos con disminución en agarre y retardo en el neurodesarrollo. El examen intraoral halló retardo de la erupción dental, hiperplasia gingival generalizada y paladar con poco crecimiento transversal. El examen radiográfico detectó órganos dentarios incluidos y mala posición en el sector anterior, molares superiores dentro del seno maxilar y caninos inferiores rotados. El paciente fue remitido a medicina para exámenes bioquímicos y genéticos para definir el diagnóstico. La bioquímica reveló MPS tipo VI, lo que fue confirmado mediante prueba molecular. Las manifestaciones clínicas en este caso corresponden a la forma clínica de progresión rápida reportada en estos pacientes: talla baja, malformaciones esqueléticas y alteraciones a nivel oral. Los niños con MPS VI grave comienzan temprano y progresan rápidamente, las radiografías óseas y la medición de GAG en orina son útiles para el diagnóstico con actividad de la enzima ARSB y genética. Es necesario fortalecer el conocimiento en odontología y la población en general sobre las características clínicas de mucopolisacáridos tipo VI para tener un diagnóstico temprano y un mejor manejo de patologías en estos pacientes.Mucopolysaccharidosis type VI, also known as Maroteaux-Lamy syndrome, is an autosomal recessive lysosomal disorder, due to the deficiency of the enzyme arylsulfatase B that leads to the accumulation of dermatan sulfate in the tissues and its urinary excretion. Mucopolysaccharide deposition leads to a progressive disorder affecting multiple organs that often results in death at a young age. This disease has several oral manifestations, among which dental complications can be serious and include follicles similar to dentigerous cysts, malocclusions, condylar defects and gingival hyperplasia, in addition to a short neck, corneal opacity, macroglossia, skull enlargement, anteroposterior dimension long, claw hand is some of the clinical features. A case of a 14-monthold patient is presented, who attended a pediatric dentistry consultation for episodes of fever, low weight, severe gingival hyperplasia. Physical examination revealed coarse facies, short neck, pectus excavatus, hands with decreased grip, and neurodevelopmental delay. On intraoral examination, dental eruption delayed, generalized gingival hyperplasia, palate with little transverse growth. On radiographic examination, dental organs included and poor position in the anterior sector, upper molars within the maxillary sinus, rotated lower canines. He is referred to medicine for biochemical tests and genetics for diagnosis. Detailed biochemistry MPS type VI, confirmed by molecular testing. The clinical manifestations in this case correspond to the clinical form of rapid progression reported in these patients. They report: short stature, skeletal malformations and alterations at the oral level. Children with severe MPS VI start early and progress rapidly, bone radiographs and urine GAG measurement are helpful for diagnosis with genetic and ARSB enzyme activity. It is necessary to strengthen the knowledge in dentistry and the general population about the clinical characteristics of type VI mucopolysaccharides in order to have an early diagnosis and management of pathologies in these patients

Engineering biocatalysts for the anaerobic recycling of toxic aromatic hydrocarbons

En: 1st Spanish National Conference on Advances in Materials Recycling and Eco – Energy Madrid, 12-13 November 2009.-- Editors: F. A. López, F. Puertas, F. J. Alguacil and A. Guerrero.-- 3 pages.Azoarcus sp. CIB is a denitrifying betaproteobacterium that uses different aromatic compounds, including toxic hydrocarbons such as toluene and m-xylene, as sole carbon sources, and is susceptible of genetic manipulation. To acquire a global view of all the genetic determinants involved in the anaerobic catabolism of aromatic compounds in strain CIB, and to accelerate and complete our understanding about this anaerobic catabolism, we have performed a genomic survey in the recently sequenced genome of this strain. This information paves the way for a pathway engineering approach within the field of anaerobic degradation of aromatics.Peer reviewe

Campylobacter jejuni en niños con enfermedad diarréica aguda (E.D.A)

During the period between September 15th, 1985 and September 30th, 1986 in Cali, Valle, Colombia, 231 children with acute diarrhea were studied looking for Carnpylobacter jejuni (CJ); 137 patients were from the Children Hospital Club Noel and the remainder from the Health Center of Siloe and the University Hospital of Cali. CJ was found in 3 1 (13.4%) children. 137 patients from Club Noel were also studied looking for salmonella, shigella and enterotoxigenic E, coli. In 13 (9.5%) patients was found only CJ. All patients had typical symptoms and signs: fever, diarrhea and feces with blood and mucous. Three patients were treated with esrtromycin. The treatment impact on it was successful in deleting the microorganisms after 72 hours. The relation boy:girl was 2:1. The most affected group compromised up to the age of 2 years, with an average of 13 months.En el período comprendido entre el 15 de septiembre de 1985 y septiembre 30 de 1986 fueron estudiados en Cali, Valle, Colombia, 231 niños que consultaron por enfermedad diarreica aguda (EDA); 137 provenían del Hospital Infantil, Club Noel y el resto del Centro de Salud de Siloé y del Hospital Universitario del Valle (HUV). El estudio de heces mostró Campylobacter jejuni (CJ) en 31 (13.4%). Los 137 pacientes del Club Noel fueron estudiados también buscando Salmonella, Shigella, E. coli enterotoxigénico, encontrándose que el CJ fue el único gérmen en 13 pacientes (9.5%) de los 19 aislamientos logrados en esta Institución. Todos los pacientes cursaron con los signos y síntomas típicos de la enfermedad; fiebre, diarrea, vómito, deposición mucosa o sanguinolenta. En cuanto al sexo hubo una relación niño:niña de 2:1 y el grupo de edad más comprometido fue hasta los 2 años, con promedio máximo hacia los 12 meses

Investigating prehistoric diet and lifeways of early farmers in central northern Spain (3000-1500 CAL BC) using stable isotope techniques

This work focuses on reconstructing past diets and animal management during Prehistory in Central Northern Spain, spanning the NE area of the Old Castilian Plateau to the Cantabrian coast, from c. 3000-1500 BCE. During this time, early farming communities made changes in their models of production and social reproduction that crystallised in the emergence of social complexity. To investigate these changes, we reconstructed the past diet of these early farming populations by using stable isotope analysis (?13C, ?15N, ?34S) of human and animal remains from the recently excavated sites of Abrigo de la Castañera in Cantabria and Arroyal I, El Hornazo, Fuente Celada and Ferrocarril-La Dehesa in Burgos. The human remains derived from a range of burial contexts including pit graves, megalithic monuments and burial caves. To provide initial insights into animal management during this timeframe, associated faunal remains were also studied as a baseline. In total, 52 samples were analysed, including 17 human burials and 35 animal specimens (cattle, sheep, pig, red deer and dog). Results show that humans in these sites consumed relatively similar diets, comprising of a predominantly C3 diet including animal protein. Animal management patterns indicate a wider use of the landscape for herbivore grazing. The differing diets of dogs at El Hornazo provide insights into the relationship that they had with humans and tentatively suggests differences in the diet of working animals versus household pets. The ?34S values of two individuals from Arroyal I indicate that they came from different regions, implying a level of inland mobility during the Chalcolithic