Noise Sources, Effects and Countermeasures in Narrowband Power-Line Communications Networks: A Practical Approach

The integration of Distributed Generation, Electric Vehicles, and storage without compromising the quality of the power delivery requires the deployment of a communications overlay that allows monitoring and controlling low voltage networks in almost real time. Power Line Communications are gaining momentum for this purpose since they present a great trade-off between economic and technical features. However, the power lines also represent a harsh communications medium which presents different problems such as noise, which is indeed affected by Distributed Generation, Electric Vehicles, and storage. This paper provides a comprehensive overview of the types of noise that affects Narrowband Power Line Communications, including normative noises, noises coming from common electronic devices measured in actual operational power distribution networks, and noises coming from photovoltaic inverters and electric vehicle charging spots measured in a controlled environment. The paper also reviews several techniques to mitigate the effects of noise, paying special attention to passive filtering, as for being one of the most widely used solution to avoid this kind of problems in the field. In addition, the paper presents a set of tests carried out to evaluate the impact of some representative noises on Narrowband Power Line Communications network performance, as well as the effectiveness of different passive filter configurations to mitigate such an impact. In addition, the considered sources of noise can also bring value to further improve PLC communications in the new scenarios of the Smart Grid as an input to theoretical models or simulations.This work has been partly funded by the Spanish Ministry of Economy and Competitiveness through the National Program for Research Aimed at the Challenges of Society under the project OSIRIS (RTC-2014-1556-3) and through the network of excellence REDYD2050 (ENE2015-70032-REDT)

Noise Sources, Effects and Countermeasures in Narrowband Power-Line Communications Networks: A Practical Approach

The integration of Distributed Generation, Electric Vehicles, and storage without compromising the quality of the power delivery requires the deployment of a communications overlay that allows monitoring and controlling low voltage networks in almost real time. Power Line Communications are gaining momentum for this purpose since they present a great trade-off between economic and technical features. However, the power lines also represent a harsh communications medium which presents different problems such as noise, which is indeed affected by Distributed Generation, Electric Vehicles, and storage. This paper provides a comprehensive overview of the types of noise that affects Narrowband Power Line Communications, including normative noises, noises coming from common electronic devices measured in actual operational power distribution networks, and noises coming from photovoltaic inverters and electric vehicle charging spots measured in a controlled environment. The paper also reviews several techniques to mitigate the effects of noise, paying special attention to passive filtering, as for being one of the most widely used solution to avoid this kind of problems in the field. In addition, the paper presents a set of tests carried out to evaluate the impact of some representative noises on Narrowband Power Line Communications network performance, as well as the effectiveness of different passive filter configurations to mitigate such an impact. In addition, the considered sources of noise can also bring value to further improve PLC communications in the new scenarios of the Smart Grid as an input to theoretical models or simulations.This work has been partly funded by the Spanish Ministry of Economy and Competitiveness through the National Program for Research Aimed at the Challenges of Society under the project OSIRIS (RTC-2014-1556-3) and through the network of excellence REDYD2050 (ENE2015-70032-REDT)

In vitro cytomodulatory and immunomodulatory effects of bovine colostrum whey protein hydrolysates

Bovine colostrum possesses biological compounds involved in the development of the newborn. Among them, the proteins have drawn attention as a source of bioactive peptides. This study shows the in vitro cytotoxic and immunostimulatory potential of two colostrum whey protein (CWP) hydrolysates obtained by in vitro digestion with pepsin and pancreatin. MTT cell viability, apoptosis induction, polymorphonuclear proliferation and phagocytic activity assays were performed. Treatment with the hydrolysates induced a significant decrease in the viability of MDA-MB-231 cell lines due to apoptosis and also a significant increase in the proliferation of blood mononuclear cells. It could also be observed that for the RM-1 and PC-3 prostate cancer cell lines and for the two times of exposure (24 and 48 h), the hydrolysate H1 is significantly more cytotoxic than CWP. These results showed the potential of bovine CWP and its hydrolysates for the treatment of chronic diseases such as cancer

Electrical Stimulation at the ST36 Acupoint Protects against Sepsis Lethality and Reduces Serum TNF Levels through Vagus Nerve- and Catecholamine-Dependent Mechanisms

Electrical vagus nerve (VN) stimulation during sepsis attenuates tumor necrosis factor (TNF) production through the cholinergic anti-inflammatory pathway, which depends on the integrity of the VN and catecholamine production. To characterize the effect of electroacupuncture at ST36 (EA-ST36) on serum TNF, IL-6, nitrite, and HMGB1 levels and survival rates, based on VN integrity and catecholamine production, a sepsis model was induced in rats using cecal ligation and puncture (CLP). The septic rats were subsequently treated with EA-ST36 (CLP+ST36), and serum samples were collected and analyzed for cytokines levels. The serum TNF, IL-6, nitrite, and HMGB1 levels in the CLP+ST36 group were significantly lower compared with the group without treatment, the survival rates were significantly higher (P<0.05), and the acute organ injury induced by CLP was mitigated by EA-ST36; however, when subdiaphragmatic vagotomy was performed, the serum levels of TNF in the CLP+ST36 group did not show a significant difference compared with the group without electrostimulation, and, similarly, no significant difference in serum TNF levels was found under the pharmacological blockade of catecholamines. These results suggest that in rats with CLP sepsis models EA-ST36 reduces serum TNF levels through VN- and atecholamine-dependent mechanisms

Salmonella Typhi Porins OmpC and OmpF Are Potent Adjuvants for T-Dependent and T-Independent Antigens

Several microbial components, such as bacterial DNA and flagellin, have been used as experimental vaccine adjuvants because of their inherent capacity to efficiently activate innate immune responses. Likewise, our previous work has shown that the major Salmonella Typhi (S. Typhi) outer membrane proteins OmpC and OmpF (porins) are highly immunogenic protective antigens that efficiently stimulate innate and adaptive immune responses in the absence of exogenous adjuvants. Moreover, S. Typhi porins induce the expression of costimulatory molecules on antigen-presenting cells through toll-like receptor canonical signaling pathways. However, the potential of major S. Typhi porins to be used as vaccine adjuvants remains unknown. Here, we evaluated the adjuvant properties of S. Typhi porins against a range of experimental and clinically relevant antigens. Co-immunization of S. Typhi porins with ovalbumin (OVA), an otherwise poorly immunogenic antigen, enhanced anti-OVA IgG titers, antibody class switching, and affinity maturation. This adjuvant effect was dependent on CD4+ T-cell cooperation and was associated with an increase in IFN-γ, IL-17A, and IL-2 production by OVA-specific CD4+ T cells. Furthermore, co-immunization of S. Typhi porins with an inactivated H1N1 2009 pandemic influenza virus experimental vaccine elicited higher hemagglutinating anti-influenza IgG titers, antibody class switching, and affinity maturation. Unexpectedly, co-administration of S. Typhi porins with purified, unconjugated Vi capsular polysaccharide vaccine (Vi CPS)—a T-independent antigen—induced higher IgG antibody titers and class switching. Together, our results suggest that S. Typhi porins OmpC and OmpF are versatile vaccine adjuvants, which could be used to enhance T-cell immune responses toward a Th1/Th17 profile, while improving antibody responses to otherwise poorly immunogenic T-dependent and T-independent antigens

Application of built-in adjuvants for epitope-based vaccines

Several studies have shown that epitope vaccines exhibit substantial advantages over conventional vaccines. However, epitope vaccines are associated with limited immunity, which can be overcome by conjugating antigenic epitopes with built-in adjuvants (e.g., some carrier proteins or new biomaterials) with special properties, including immunologic specificity, good biosecurity and biocompatibility, and the ability to vastly improve the immune response of epitope vaccines. When designing epitope vaccines, the following types of built-in adjuvants are typically considered: (1) pattern recognition receptor ligands (i.e., toll-like receptors); (2) virus-like particle carrier platforms; (3) bacterial toxin proteins; and (4) novel potential delivery systems (e.g., self-assembled peptide nanoparticles, lipid core peptides, and polymeric or inorganic nanoparticles). This review primarily discusses the current and prospective applications of these built-in adjuvants (i.e., biological carriers) to provide some references for the future design of epitope-based vaccines

Estudio de la respuesta inmune e inmunidad generada por la porina omps2 de salmonella entérica serovar typhi

Tesis (Maestría en Ciencias en Inmunología), Instituto Politécnico Nacional, SEPI, ENCB, 2008, 1 archivo PDF, (79 páginas). tesis.ipn.m

Modulation of immune response by bacterial lipopolysaccharides

Lipopolysaccharide (LPS) is a molecule that is profusely found on the outer membrane of Gram-negative bacteria and is also a potent stimulator of the immune response. As the main molecule on the bacterial surface, is also the most biologically active. The immune response of the host is activated by the recognition of LPS through Toll-like receptor 4 (TLR4) and this receptor-ligand interaction is closely linked to LPS structure. Microorganisms have evolved systems to control the expression and structure of LPS, producing structural variants that are used for modulating the host immune responses during infection. Examples of this include Helicobacter pylori, Francisella tularensis, Chlamydia trachomatis and Salmonella spp. High concentrations of LPS can cause fever, increased heart rate and lead to septic shock and death. However, at relatively low concentrations some LPS are highly active immunomodulators, which can induce non-specific resistance to invading microorganisms. The elucidation of the molecular and cellular mechanisms involved in the recognition of LPS and its structural variants has been fundamental to understand inflammation and is currently a pivotal field of research to understand the innate immune response, inflammation, the complex host-pathogen relationship and has important implications for the rational development of new immunomodulators and adjuvants

Altered Expression of Natural Cytotoxicity Receptors and NKG2D on Peripheral Blood NK Cell Subsets in Breast Cancer Patients

AbstractHuman natural killer (NK) cells are considered professional cytotoxic cells that are integrated into the effector branch of innate immunity during antiviral and antitumoral responses. The purpose of this study was to examine the peripheral distribution and expression of NK cell activation receptors from the fresh peripheral blood mononuclear cells of 30 breast cancer patients prior to any form of treatment (including surgery, chemotherapy, and radiotherapy), 10 benign breast pathology patients, and 24 control individuals. CD3−CD56dimCD16bright NK cells (CD56dim NK) and CD3−CD56brightCD16dim/− NK cells (CD56bright NK) were identified using flow cytometry. The circulating counts of CD56dim and CD56bright NK cells were not significantly different between the groups evaluated, nor were the counts of other leukocyte subsets between the breast cancer patients and benign breast pathology patients. However, in CD56dim NK cells, NKp44 expression was higher in breast cancer patients (P = .0302), whereas NKp30 (P = .0005), NKp46 (P = .0298), and NKG2D (P = .0005) expression was lower with respect to healthy donors. In CD56bright NK cells, NKp30 (P = .0007), NKp46 (P = .0012), and NKG2D (P = .0069) expression was lower in breast cancer patients compared with control group. Only NKG2D in CD56bright NK cells (P = .0208) and CD56dim NK cells (P = .0439) showed difference between benign breast pathology and breast cancer patients. Collectively, the current study showed phenotypic alterations in activation receptors on CD56dim and CD56bright NK cells, suggesting that breast cancer patients have decreased NK cell cytotoxicity