297 research outputs found

    ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI

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    ONTOGENY AND PHYLOGENETIC SYSTEMATICS

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    Dedifferentiation, paedomorphosis, and the insertion and deletion of developmental stages make it impossible to deduce the genealogical hierarchy from only ontogenetic transformation series. Like the outgroup criterion, ontogenetic character precedence is not theory-neutral and to use it to deduce genealogy requires certain assumptions. If scientists are going to use logically unbeatable theories about the world, they might as well give up natural science and take up religion (Lewontin, 1972: 181).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73749/1/j.1096-0031.1985.tb00408.x.pd

    Isolation and Chemical Composition of Dinoflagellate Nuclei *

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    Nuclei were isolated from Peridinium cinctum. Peridinium trochoideum, Gyrodinium cohnii ( Cryptothecodinium cohnii ) and Gymnodinium nelsoni. The nuclei of G. cohnii, P. trochoideum and G. nelsoni were found to contain ∼ 6.9, 34 and 143 picograms of DNA respectively. The ratios to DNA of RNA, acid-soluble and acid-insoluble protein for G. cohnii were 0.32, 0.13 and 0.99, respectively. The corresponding values for P. trochoideum were 0.22, 0.08 and 1.22, while those for G. nelsoni were 0.21, 0.10 and 1.09. The chemical composition of dinoflagellate nuclei is compared and contrasted with that of typical eukaryotic nuclei. The culture of these difficult organisms also is discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72006/1/j.1550-7408.1973.tb03595.x.pd

    Functional bases of fiber length and angulation in muscle

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    The differences in angulation and length observed for the fibers of anatomical muscles may reflect two distinct mechanical requirements: (1) arrangement for pinnation, reflecting an increase in physiological crosssection and (2) arrangement for equivalent placement of sarcomeres, possibly associated with coordination. The observed differences in fiber angulation and length have different effects upon the responses of sarcomeres, specifically on their extent and rate of shortening and on the force they may generate. The basic mechanisms governing these effects and the various arrangements of muscles are reviewed. Fiber length and angulation in the complex M. adductor mandibulae externus 2 of a lizard were measured stereotactically; these values correlace well with the hypothesis that the muscle shows equivalence and demonstrate that angulation for pinnation is less constant. An outline for the study of muscle architecture and function, detailing the kinds of information required to estimate forces and evaluate muscle and fiber placements, is presented.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50282/1/1051920106_ftp.pd
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