Construction of 2-factors in the middle layer of the discrete cube

Define the middle layer graph as the graph whose vertex set consists of all bitstrings of length $2n+1$ that have exactly $n$ or $n+1$ entries equal to 1, with an edge between any two vertices for which the corresponding bitstrings differ in exactly one bit. In this work we present an inductive construction of a large family of 2-factors in the middle layer graph for all $n\geq 1$. We also investigate how the choice of certain parameters used in the construction affects the number and lengths of the cycles in the resulting 2-factor

Experimental evidence in support of single host maintenance of a multihost pathogen

Copyright: 2014 Duffus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/3.0

In Search of the "Telling Detail": Ian McEwan, Briony Tallis, and the Demands of Authorship

Much scholarly interest surrounding Ian McEwan's Atonement has focused on the abrupt shift that occurs in the novel's final section, "London, 1999." This essay argues that this section makes it clear that the main story of the novel is not Briony, Robbie, and Cecilia's entanglement due to Lola's teenaged rape but Briony's development as a writer, her kunstlerroman. As such, it is crucial to the novel, not simply a metafictional ploy, because it illuminates the lengths she has gone to in writing her final book and fulfilling her youthful promise. McEwan's response to a real-life plagiarism accusation reinforces his depiction of Briony as an author who searches for "the telling detail," as opposed to one who sticks to verifiable, historical accuracy

Functional studies on oligotropha carboxidovorans molybdenum–copper CO dehydrogenase produced in escherichia coli

The Mo/Cu-dependent CO dehydrogenase (CODH) from Oligotropha carboxidovorans is an enzyme that is able to catalyze both the oxidation of CO to CO2 and the oxidation of H2 to protons and electrons. Despite the close to atomic resolution structure (1.1 Å), significant uncertainties have remained with regard to the reaction mechanism of substrate oxidation at the unique Mo/Cu center, as well as the nature of intermediates formed during the catalytic cycle. So far, the investigation of the role of amino acids at the active site was hampered by the lack of a suitable expression system that allowed for detailed site-directed mutagenesis studies at the active site. Here, we report on the establishment of a functional heterologous expression system of O. carboxidovorans CODH in Escherichia coli. We characterize the purified enzyme in detail by a combination of kinetic and spectroscopic studies and show that it was purified in a form with characteristics comparable to those of the native enzyme purified from O. carboxidovorans. With this expression system in hand, we were for the first time able to generate active-site variants of this enzyme. Our work presents the basis for more detailed studies of the reaction mechanism for CO and H2 oxidation of Mo/Cu-dependent CODHs in the future

Feminist Theory and Technical Communication

This essay explores feminism, socially-constructed norms, and the relationship between feminism and technical communication. It argues that undergraduate technical communication programs should include courses that study feminist history and theories as related to the field, claiming that studying feminist theory will improve user-centered design and broaden students\u27 spheres of influence as professionals