292 research outputs found
Membrane-protein interactions in mechanosensitive channels
In this paper, we examine the mechanical role of the lipid bilayer in ion
channel conformation and function with specific reference to the case of the
mechanosensitive channel of large conductance (MscL). In a recent paper
(Wiggins and Phillips, 2004), we argued that mechanotransduction very naturally
arises from lipid-protein interactions by invoking a simple analytic model of
the MscL channel and the surrounding lipid bilayer. In this paper, we focus on
improving and expanding this analytic framework for studying lipid-protein
interactions with special attention to MscL. Our goal is to generate simple
scaling relations which can be used to provide qualitative understanding of the
role of membrane mechanics in protein function and to quantitatively interpret
experimental results. For the MscL channel, we find that the free energies
induced by lipid-protein interaction are of the same order as the free energy
differences between conductance states measured by Sukharev et al. (1999). We
therefore conclude that the mechanics of the bilayer plays an essential role in
determining the conformation and function of the channel. Finally, we compare
the predictions of our model to experimental results from the recent
investigations of the MscL channel by Perozo et al. (2002), Powl et al. (2003),
Yoshimura et al. (2004), and others and suggest a suite of new experiments
Analytic models for mechanotransduction: gating a mechanosensitive channel
Analytic estimates for the forces and free energy generated by bilayer
deformation reveal a compelling and intuitive model for MscL channel gating
analogous to the nucleation of a second phase. We argue that the competition
between hydrophobic mismatch and tension results in a surprisingly rich story
which can provide both a quantitative comparison to measurements of opening
tension for MscL when reconstituted in bilayers of different thickness and
qualitative insights into the function of the MscL channel and other
transmembrane proteins
Targeted free energy perturbation
A generalization of the free energy perturbation identity is derived, and a
computational strategy based on this result is presented. A simple example
illustrates the efficiency gains that can be achieved with this method.Comment: 8 pages + 1 color figur
Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution
Mechanosensitive (MS) ion channels are membrane proteins that detect and respond to membrane tension in all branches of life. In bacteria, MS channels prevent cells from lysing upon sudden hypoosmotic shock by opening and releasing solutes and water. Despite the importance of MS channels and ongoing efforts to explain their functioning, the molecular mechanism of MS channel gating remains elusive and controversial. Here we report a method that allows single-subunit resolution for manipulating and monitoring “mechanosensitive channel of large conductance” from Escherichia coli. We gradually changed the hydrophobicity of the pore constriction in this homopentameric protein by modifying a critical pore residue one subunit at a time. Our experimental results suggest that both channel opening and closing are initiated by the transmembrane 1 helix of a single subunit and that the participation of each of the five identical subunits in the structural transitions between the closed and open states is asymmetrical. Such a minimal change in the pore environment seems ideal for a fast and energy-efficient response to changes in the membrane tension.
Gating-by-tilt of mechanosensitive membrane channels
We propose an alternative mechanism for the gating of biological membrane
channels in response to membrane tension that involves a change in the slope of
the membrane near the channel. Under biological membrane tensions we show that
the energy difference between the closed (tilted) and open (untilted) states
can far exceed kBT and is comparable to what is available under simple
ilational gating. Recent experiments demonstrate that membrane leaflet
asymmetries (spontaneous curvature) can strong effect the gating of some
channels. Such a phenomenon would be more easy to explain under gating-by-tilt,
given its novel intrinsic sensitivity to such asymmetry.Comment: 10 pages, 2 figure
Alpha helix-coil phase transition: analysis of ab initio theory predictions
In the present paper we present results of calculations obtained with the use
of the theoretical method described in our preceding paper [1] and perform
detail analysis of alpha helix-random coil transition in alanine polypeptides
of different length. We have calculated the potential energy surfaces of
polypeptides with respect to their twisting degrees of freedom and construct a
parameter-free partition function of the polypeptide using the suggested method
[1]. From the build up partition function we derive various thermodynamical
characteristics for alanine polypeptides of different length as a function of
temperature. Thus, we analyze the temperature dependence of the heat capacity,
latent heat and helicity for alanine polypeptides consisting of 21, 30, 40, 50
and 100 amino acids. Alternatively, we have obtained same thermodynamical
characteristics from the use of molecular dynamics simulations and compared
them with the results of the new statistical mechanics approach. The comparison
proves the validity of the statistical mechanic approach and establishes its
accuracy.Comment: 34 pages, 12 figure
Vibrational Spectra of a Mechanosensitive Channel
We report the simulated vibrational spectra of a mechanosensitive membrane channel in different gating states. Our results show that while linear absorption is insensitive to structural differences, linear dichroism and sum-frequency generation spectroscopies are sensitive to the orientation of the transmembrane helices, which is changing during the opening process. Linear dichroism cannot distinguish an intermediate structure from the closed structure, but sum-frequency generation can. In addition, we find that two-dimensional infrared spectroscopy can be used to distinguish all three investigated gating states of the mechanosensitive membrane channel.
Clonal karyotype evolution involving ring chromosome 1 with myelodysplastic syndrome subtype RAEB-t progressing into acute leukemia
s Karyotypic evolution is a well-known phenomenon in patients with malignant hernatological disorders during disease progression. We describe a 50-year-old male patient who had originally presented with pancytopenia in October 1992. The diagnosis of a myelodysplastic syndrome (MDS) FAB subtype RAEB-t was established in April 1993 by histological bone marrow (BM) examination, and therapy with low-dose cytosine arabinoside was initiated. In a phase of partial hernatological remission, cytogenetic assessment in August 1993 revealed a ring chromosome 1 in 13 of 21 metaphases beside BM cells with normal karyotypes {[}46,XY,r(1)(p35q31)/46,XY]. One month later, the patient progressed to an acute myeloid leukemia (AML), subtype M4 with 40% BM blasts and cytogenetic examination showed clonal evolution by the appearance of additional numerical aberrations in addition to the ring chromosome{[}46,XY,r(1),+8,-21/45,XY,r(1),+8,-21,-22/46, XY]. Intensive chemotherapy and radiotherapy was applied to induce remission in preparation for allogeneic bone marrow transplantation (BMT) from the patient's HLA-compatible son. After BMT, complete remission was clinically, hematologically and cytogenetically (normal male karyotype) confirmed. A complete hematopoietic chimerism was demonstrated. A relapse in January 1997 was successfully treated using donor lymphocyte infusion and donor peripheral blood stem cells (PB-SC) in combination with GM-CSF as immunostimulating agent in April 1997, and the patient's clinical condition remained stable as of January 2005. This is an interesting case of a patient with AML secondary to MDS. With the ring chromosome 1 we also describe a rare cytogenetic abnormality that predicted the poor prognosis of the patient, but the patient could be cured by adoptive immunotherapy and the application of donor's PB-SC. This case confirms the value of cytogenetic analysis in characterizing the malignant clone in hernatological neoplasias, the importance of controlling the quality of an induced remission and of the detection of a progress of the disease. Copyright (c) 2006 S. Karger AG, Basel
In situ, Reversible Gating of a Mechanosensitive Ion Channel through Protein-Lipid Interactions
Understanding the functioning of ion channels, as well as utilizing their properties for biochemical applications requires control over channel activity. Herein we report a reversible control over the functioning of a mechanosensitive ion channel by interfering with its interaction with the lipid bilayer. The mechanosensitive channel of large conductance from Escherichia coli is reconstituted into liposomes and activated to its different sub-open states by titrating lysophosphatidylcholine (LPC) into the lipid bilayer. Activated channels are closed back by the removal of LPC out of the membrane by bovine serum albumin (BSA). Electron paramagnetic resonance spectra showed the LPC-dose-dependent gradual opening of the channel pore in the form of incrementally increasing spin label mobility and decreasing spin-spin interaction. A method to reversibly open and close mechanosensitive channels to distinct sub-open conformations during their journey from the closed to the fully open state enables detailed structural studies to follow the conformational changes during channel functioning. The ability of BSA to revert the action of LPC opens new perspectives for the functional studies of other membrane proteins that are known to be activated by LPC
An improved open-channel structure of MscL determined from FRET confocal microscopy and simulation
Mechanosensitive channels act as molecular transducers of mechanical force exerted on the membrane of living cells by opening in response to membrane bilayer deformations occurring in physiological processes such as touch, hearing, blood pressure regulation, and osmoregulation. Here, we determine the likely structure of the open state of the mechanosensitive channel of large conductance using a combination of patch clamp, fluorescence resonance energy transfer (FRET) spectroscopy, data from previous electron paramagnetic resonance experiments, and molecular and Brownian dynamics simulations. We show that structural rearrangements of the protein can be measured in similar conditions as patch clamp recordings while controlling the state of the pore in its natural lipid environment by modifying the lateral pressure distribution via the lipid bilayer. Transition to the open state is less dramatic than previously proposed, while the N terminus remains anchored at the surface of the membrane where it can either guide the tilt of or directly translate membrane tension to the conformation of the pore-lining helix. Combining FRET data obtained in physiological conditions with simulations is likely to be of great value for studying conformational changes in a range of multimeric membrane proteins
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