Assessment of the susceptibility to pests and diseases of 36 apple cultivars in four low-input organic orchards in France

One of the keystones of the organic orchard is the cultivar choice as one element of pest and disease control. However, few exhaustive data sets concerning the cultivar susceptibility to pests and diseases are available for growers. In order to identify cultivars adapted to organic production methods, the susceptibility to scab, aphids and powdery mildew and the agronomic properties of 36 cultivars were assessed in four French sites under different pedo-climatic conditions. Different levels of susceptibility to scab were observed for 23 scab Vf-resistant and 13 other cultivars. In the North of France where Vf resistance is overcome, the Vf cultivars displayed different levels of scab severity. A high variability in the susceptibility to aphids was observed whereas susceptibility to powdery mildew varied less. The analysis of susceptibility properties, yield and fruit quality, fruit storability and tree behaviour permitted to identify a set of interesting cultivars according to the site

Aliphatic isocyanurates and polyisocyanurate networks

The production, processing, and application of aliphatic isocyanate (NCO)-based thermosets such as polyurethane coatings and adhesives are generally limited by the surprisingly high viscosity of tri-functionality and higher-functionality isocyanurates. These compounds are essential crosslinking additives for network formation. However, the mechanism by which these high viscosities are caused is not yet understood. In this work, model aliphatic isocyanurates were synthesized and isolated in high purity (>99%), and their viscosities were accurately determined. It was shown that the presence of the NCO group has a strong influence on the viscosity of the system. From density functional theory calculations, a novel and significant bimolecular binding potential of À8.7 kJ/mol was identified be- tween NCO groups and isocyanurate rings, confirming the important role of the NCO group. This NCO-to-ring inter- action was proposed to be the root cause for the high viscosities observed for NCO-functional isocyanurate systems. Molecular dynamics simulations carried out to further confirm this influence also suggest that the NCO-to- ring interaction causes a significant additional contribution to viscosity. Finally, model functional isocyanurates were further reacted into densely crosslinked polyisocyanurate networks which showed interesting material properties.This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 642890 (http:// thelink-project.eu/), and it was partially supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the strategic funding UID/FIS/04650/2013, and under the project Search-ON2: NORTE-07-0162-FEDER000086

ÉVALUATION DE LA SENSIBILITÉ AUX BIOAGRESSEURS DE 36 VARIÉTÉS DE POMMIERS DANS UN RÉSEAU DE PARCELLES BIOLOGIQUES À FAIBLES INTRANTS

Les bioagresseurs sont une des principales limites à la production de pomme dans les systèmes conduits en AB et à faibles niveaux d’intrants. Une des clefs du contrôle des bioagresseurs dans ces vergers est le choix des variétés (Trapman et Jansonius, 2008). La variabilité de l’expression de la sensibilité variétale peut être appréhendée grâce à un réseau multi-site pluri-annuel. Quatre parcelles de pommier biologique conduites de manière semi-extensive ont été plantées en 2002 afin d’identifier des variétés d’intérêts pour les systèmes à faible intrants. 36 variétés ont été suivies afin d’évaluer conjointement leur sensibilité à la tavelure, aux pucerons et à l’oïdium.La thématique de cette étude initiée en 2002 se trouve être au coeur des questionnements récents sur la réduction des intrants en arboriculture (programme ECOPHYTO 2018). Cette étude souligne (1) la spécificité des variétés intéressantes pour un site donné, (2) l’apparation dans certains cas de maladies considérées comme anecdoctiques, (3) l’intérêt de dispositifs multi-sites et d’observations pluriannuelles pour évaluer avec fiabilité la sensibilité aux bioagresseurs, (4) la méthodologie adaptée à cette problématique (méthode de notation, type de dispositif, etc)

Les principales clés du verger bio transfrontalier : Pommes et poires, une approche globale

TransBioFruit existe parce qu’il y a une demande ! Les arboriculteurs biologiques ont été à l’origine de ce projet en sollicitant des réponses aux questions techniques qui se posent. Ils se sont investis dans le déroulement des programmes en accueillant des expérimentations et en partageant leur expérience. Si la production de fruits biologiques est aujourd’hui reconnue comme partie prenante d’une démarche de développement durable et si elle est l’objet d’une demande des consommateurs en augmentation constante, sa mise en pratique ne va pas de soi. Les arboriculteurs biologiques sont, chaque jour, confrontés à des questions sur la manière de maintenir un état sanitaire satisfaisant de leurs vergers dans le respect du cahier des charges européen de l’agriculture biologique. Pour se développer et être économiquement rentable, l’agriculture biologique doit s’appuyer sur toutes les techniques de production les plus modernes, à la fois innovantes et respectueuses de l’environnement. Pour peu que les producteurs adoptent une approche globale, les solutions développées dans ce projet sont transférables aux arboriculteurs conventionnels qui souhaitent diminuer leurs utilisations de produits chimiques de synthèse. De manière générale, répondre à ces problématiques permet aussi d’accompagner et d’anticiper les évolutions des réglementations européennes de plus en plus restrictives concernant l’utilisation des produits phytosanitaires en agriculture

Development of solvent-free synthesis of hydrogen-bonded supramolecular polyurethanes

In this work we describe the solvent free synthesis of supramolecular polyurethanes held together by heterocomplementary triple hydrogen bonding. We perform a systematic evaluation on the base catalyzed synthesis of small molecule ureas and carbamates from a range of isocyanates, amines and alcohols in solution and in the solid state using ball milling. These optimized procedures are then shown to be applicable to the synthesis of supramolecular polyurethanes using solvent-free method

An adhesive elastomeric supramolecular polyurethane healable at body temperature

In this paper, we report the synthesis and healing ability of a non-cytotoxic supramolecular polyurethane network whose mechanical properties can be recovered efficiently (> 99%) at the temperature of the human body (37 ºC). Rheological analysis revealed an acceleration in the drop of the storage modulus above 37 ºC, on account of the dissociation of the supramolecular polyurethane network, and this decrease in viscosity enables the efficient recovery of the mechanical properties. Microscopic and mechanical characterisation has shown that this material is able to recover mechanical properties across a damage site with minimal contact required between the interfaces and also demonstrated that the mechanical properties improved when compared to other low temperature healing elastomers or gel-like materials. The supramolecular polyurethane was found to be non-toxic in a cytotoxicity assay carried out in human skin fibroblasts (cell viability > 94% and non-significantly different compared to the untreated control). This supramolecular network material also exhibited excellent adhesion to pig skin and could be healed completely in situ post damage indicating that biomedical applications could be targeted, such as artificial skin or wound dressings with supramolecular materials of this type

Plant selection for rain gardens : response to simulated cyclical flooding of 15 perennial species

Plant selection for rain gardens can be complicated, as cyclic flooding and a gradient of moisture level are expected in the depression structure of a rain garden. However, few studies to date have quantified how plant establishment is affected by rain garden moisture dynamics. This study investigated tolerance of 15 candidate perennial species, which experienced flooding cycles consisting of 1-day and 4-day inundation and draining phases. In this study, detection of species suitability using survival and growth measurements coupled with the stress indicator (i.e. chlorophyll fluorescence) provided a valid framework for wider use in plant selection for rain gardens. The methodology is also confident in predicting the possible placing in different plant moisture zones. All species survived the cyclic flooding treatments and grew to their maximum. Photosynthesis and physical growth in only a few candidate species (e.g. Amsonia tabernaemontana var. salicifolia, Gaura lindheimeri, Sanguisorba tenuifolia ‘Purpurea’ and Thalictrum aquilegifolium) tended to be inhibited by treatments adopting 4-day cyclic flooding, whilst tolerance to 1-day cyclic flooding was clearly demonstrated in most species. Analysis suggests that most species assumed to withstand infrequent to periodic inundation, such as Iris sibirica, Filipendula purpurea and Miscanthus sinensis, are resilient species and are sensible for use in a wider range of rain garden moisture conditions from damp depression bottom to dry margin. Species assumed to be intolerant of inundation such as Gaura lindheimeri may be successful in the rain garden environment, but they are recommended for the dryer zones

Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential

Matrix metalloproteinases (Mmps) stimulate tumor invasion and metastasis by degrading the extracellular matrix. Here we reveal an unexpected role for Mmp10 (stromelysin 2) in the maintenance and tumorigenicity of mouse lung cancer stem-like cells (CSC). Mmp10 is highly expressed in oncosphere cultures enriched in CSCs and RNAi-mediated knockdown of Mmp10 leads to a loss of stem cell marker gene expression and inhibition of oncosphere growth, clonal expansion, and transformed growth in vitro. Interestingly, clonal expansion of Mmp10 deficient oncospheres can be restored by addition of exogenous Mmp10 protein to the culture medium, demonstrating a direct role for Mmp10 in the proliferation of these cells. Oncospheres exhibit enhanced tumor-initiating and metastatic activity when injected orthotopically into syngeneic mice, whereas Mmp10-deficient cultures show a severe defect in tumor initiation. Conversely, oncospheres implanted into syngeneic non-transgenic or Mmp10−/− mice show no significant difference in tumor initiation, growth or metastasis, demonstrating the importance of Mmp10 produced by cancer cells rather than the tumor microenvironment in lung tumor initiation and maintenance. Analysis of gene expression data from human cancers reveals a strong positive correlation between tumor Mmp10 expression and metastatic behavior in many human tumor types. Thus, Mmp10 is required for maintenance of a highly tumorigenic, cancer-initiating, metastatic stem-like cell population in lung cancer. Our data demonstrate for the first time that Mmp10 is a critical lung cancer stem cell gene and novel therapeutic target for lung cancer stem cells