Optimization-based comparison of different approaches for the automatized calculation of residual stresses and fiber orientations in arteries

Residual stresses and fiber orientations in arterial walls can be approximated by means of the simulation of growth and remodeling processes. In order to enable a comparison of different approaches of combined growth and remodeling in one framework, a method based on the optimization of model parameters is developed. The minimization of a mechano-biologically motivated objective function permits to evaluate the approaches with respect to their ability of effectively reducing stress peaks and stress inhomogeneities in the arterial wall. This examination is performed for a simplified, one-layered, rotationally symmetric arterial segment in order to enable the analysis of the fundamental mechanisms included in the individual model variants. Once the most probable growth mechanism is identified, multi-layered segments can be analyzed in more detail

MODELING OF ANISOTROPIC GROWTH AND RESIDUAL STRESSES IN ARTERIAL WALLS

Based on the multiplicative decomposition of the deformation gradient, a local formulation for anisotropic growth in soft biological tissues is formulated by connecting the growth tensor to the main anisotropy directions. In combination with an anisotropic driving force, the model enables an effective stress reduction due to growth-induced residual stresses. A method for the imitation of opening angle experiments in numerically simulated arterial segments, visualizing the deformations related to residual stresses, is presented and illustrated in a numerical example

Residual stresses resulting from growth and remodeling in arterial walls

A model for multiplicative anisotropic growth in soft biological tissues, which relates the growth tensor to the fibrous tissue structure, is combined with a fiber remode- ling framework. Both adaptation mechanisms are supposed to be governed by the intensity and the directions of the tensile principal stresses. Numerical examples on idealized arterial segments, illustrating stress and fiber angle distributions as well as resulting residual stresses in cases with and without fiber remodeling, are presented. It turns out that all processes including growth and remodeling are necessary to obtain qualitatively realistic distributions of fiber orientations, residual stresses, and stresses under loading

Thin films with high surface roughness: thickness and dielectric function analysis using spectroscopic ellipsometry

An optical surface roughness model is presented, which allows a reliable determination of the dielectric function of thin films with high surface roughnesses of more than 10 nm peak to valley distance by means of spectroscopic ellipsometry. Starting from histogram evaluation of atomic force microscopy (AFM) topography measurements a specific roughness layer (RL) model was developed for an organic thin film grown in vacuum which is well suited as an example. Theoretical description based on counting statistics allows generalizing the RL model developed to be used for all non-conducting materials. Finally, a direct input of root mean square (RMS) values found by AFM measurements into the proposed model is presented, which is important for complex ellipsometric evaluation models where a reduction of the amount of unknown parameters can be crucial. Exemplarily, the evaluation of a N,N’-dimethoxyethyl-3,4,9,10-perylene-tetracarboxylic-diimide (DiMethoxyethyl-PTCDI) film is presented, which exhibits a very high surface roughness, i.e. showing no homogeneous film at all

Remote detection of resonant circuits in the 55-90 MHz range

Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1995.Includes bibliographical references (p. 55).by Daniel Jacob Zahn.M.Eng

Electrical microcurrent to prevent conditioning film and bacterial adhesion to urological stents

Long-term catheters remain a significant clinical problem in urology due to the high rate of bacterial colonization, infection, and encrustation. Minutes after insertion of a catheter, depositions of host urinary components onto the catheter surface form a conditioning film actively supporting the bacterial adhesion process. We investigated the possibility of reducing or avoiding the buildup of these naturally forming conditioning films and of preventing bacterial adhesion by applying different current densities to platinum electrodes as a possible catheter coating material. In this model we employed a defined environment using artificial urine and Proteus mirabilis. The film formation and desorption was analyzed by highly mass sensitive quartz crystal microbalance and surface sensitive atomic force microscopy. Further, we performed bacterial staining to assess adherence, growth, and survival on the electrodes with different current densities. By applying alternating microcurrent densities on platinum electrodes, we could produce a self regenerative surface which actively removed the conditioning film and significantly reduced bacterial adherence, growth, and survival. The results of this study could easily be adapted to a catheter design for clinical us

Studying hydrogen bonding and dynamics of the acetylate groups of the Special Pair of Rhodobacter sphaeroides WT

Although the cofactors in the bacterial reaction centre of Rhodobacter sphaeroides wild type (WT) are arranged almost symmetrically in two branches, the light-induced electron transfer occurs selectively in one branch. As origin of this functional symmetry break, a hydrogen bond between the acetyl group of PL in the primary donor and His-L168 has been discussed. In this study, we investigate the existence and rigidity of this hydrogen bond with solid-state photo-CIDNP MAS NMR methods offering information on the local electronic structure due to highly sensitive and selective NMR experiments. On the time scale of the experiment, the hydrogen bond between PL and His-L168 appears to be stable and not to be affected by illumination confirming a structural asymmetry within the Special Pair

Studying hydrogen bonding and dynamics of the acetylate groups of the Special Pair of Rhodobacter sphaeroides WT

Although the cofactors in the bacterial reaction centre of Rhodobacter sphaeroides wild type (WT) are arranged almost symmetrically in two branches, the light-induced electron transfer occurs selectively in one branch. As origin of this functional symmetry break, a hydrogen bond between the acetyl group of PL in the primary donor and His-L168 has been discussed. In this study, we investigate the existence and rigidity of this hydrogen bond with solid-state photo-CIDNP MAS NMR methods offering information on the local electronic structure due to highly sensitive and selective NMR experiments. On the time scale of the experiment, the hydrogen bond between PL and His-L168 appears to be stable and not to be affected by illumination confirming a structural asymmetry within the Special Pair

Measuring telomere length and telomere dynamics in evolutionary biology and ecology

Telomeres play a fundamental role in the protection of chromosomal DNA and in the regulation of cellular senescence. Recent work in human epidemiology and evolutionary ecology suggests adult telomere length (TL) may reflect past physiological stress and predict subsequent morbidity and mortality, independent of chronological age. Several different methods have been developed to measure TL, each offering its own technical challenges. The aim of this review is to provide an overview of the advantages and drawbacks of each method for researchers, with a particular focus on issues that are likely to face ecologists and evolutionary biologists collecting samples in the field or in organisms that may never have been studied in this context before. We discuss the key issues to consider and wherever possible try to provide current consensus view regarding best practice with regard to sample collection and storage, DNA extraction and storage, and the five main methods currently available to measure TL. Decisions regarding which tissues to sample, how to store them, how to extract DNA, and which TL measurement method to use cannot be prescribed, and are dependent on the biological question addressed and the constraints imposed by the study system. What is essential for future studies of telomere dynamics in evolution and ecology is that researchers publish full details of their methods and the quality control thresholds they employ

New Constraints on the Composition of Jupiter from Galileo Measurements and Interior Models

Using the helium abundance measured by Galileo in the atmosphere of Jupiter and interior models reproducing the observed external gravitational field, we derive new constraints on the composition and structure of the planet. We conclude that, except for helium which must be more abundant in the metallic interior than in the molecular envelope, Jupiter could be homogeneous (no core) or could have a central dense core up to 12 Earth masses. The mass fraction of heavy elements is less than 7.5 times the solar value in the metallic envelope and between 1 and 7.2 times solar in the molecular envelope. The total amount of elements other than hydrogen and helium in the planet is between 11 and 45 Earth masses.Comment: 15 pages, 2 figures (1 color