Subpoblaciones linfocitarias de pacientes venezolanos infectados con SARS-CoV-2

La infección por SARS-CoV-2 ha infectado a más de 50 millones de personas con un rango de letalidad de 3 %-10 % de los casos. En Venezuela, cerca de 95 mil casos se han reportado con una letalidad del 1 %. El objetivo de este estudio es evaluar las diferentes poblaciones linfocitarias, analizar los posibles marcadores pronósticos y compararlos con la literatura.The SARS-CoV-2 infection has infected more than 50 million people with the lethality of 3 %-10 % of the cases. In Venezuela, around 95 thousand individuals have been found positive with the lethality of around 1 %. Our study aimed to ascertain the different lymphocyte cell populations, analyze possible prognostic markers, and compare them with the literature

Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression

Renal DNase I is lost in advanced stages of lupus nephritis. Here, we determined if loss of renal DNase I reflects a concurrent loss of urinary DNase I, and whether absence of urinary DNase I predicts disease progression. Mouse and human DNase I protein and DNase I endonuclease activity levels were determined by western blot, gel, and radial activity assays at different stages of the murine and human forms of the disease. Cellular localization of DNase I was analyzed by immunohistochemistry, immunofluorescence, confocal microscopy, and immunoelectron microscopy. We further compared DNase I levels in human native and transplanted kidneys to determine if the disease depended on autologous renal genes, or whether the nephritic process proceeded also in transplanted kidneys. The data indicate that reduced renal DNase I expression level relates to serious progression of lupus nephritis in murine, human native, and transplanted kidneys. Notably, silencing of renal DNase I correlated with loss of DNase I endonuclease activity in the urine samples. Thus, urinary DNase I levels may therefore be used as a marker of lupus nephritis disease progression and reduce the need for renal biopsies