A completely automated high inoculation density fed batch process that accommodates clonal diversity and routinely doubles space time yield as compared to low inoculation density processes

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The RB1 Story: Characterization and Cloning of the First Tumor Suppressor Gene.

The RB1 gene is the first described human tumor suppressor gene and plays an integral role in the development of retinoblastoma, a pediatric malignancy of the eye. Since its discovery, the stepwise characterization and cloning of RB1 have laid the foundation for numerous advances in the understanding of tumor suppressor genes, retinoblastoma tumorigenesis, and inheritance. Knowledge of RB1 led to a paradigm shift in the field of cancer genetics, including widespread acceptance of the concept of tumor suppressor genes, and has provided crucial diagnostic and prognostic information through genetic testing for patients affected by retinoblastoma. This article reviews the long history of RB1 gene research, characterization, and cloning, and also discusses recent advances in retinoblastoma genetics that have grown out of this foundational work

The Identification of Diffuse Bands

Our present knowledge of the diffuse interstellar bands (two of which were first noted by Wilson in 1958) is briefly summarized. Other broad and very broad interstellar features (the 220nm extinction bump, the very broad structure in the extinction curve, the extended red emission in reflection and other nebulae, and the unidentified - sometimes called the "overidentified" - infrared bands) are also briefly described. The origins of all these features remain unknown, in spite of intensive study. Possible relations between these various features and families is briefly discussed. Recent observations are shown to support the hypothesis that the carriers of the diffuse bands are free-flying molecules, whereas the alternative hypothesis of dust grains now seems to be untenable. Candidate types of molecular carriers are mentioned, and the possible source of such molecules in the diffuse interstellar medium is briefly discussed