Distribution of an Exotic Pest, \u3ci\u3eAgromyza Frontella\u3c/i\u3e (Diptera: Agromyzidae), in Manitoba, Canada.

Agromyza frontella is an exotic alfalfa pest from Europe that was first detected in North America in 1968 and has since spread westward into Ontario and the north central United States. Informal surveys had detected A. frontella in Manitoba, but its distribution throughout this province was unknown. In 1998 we collected alfalfa stems to detect plant damage and sweep samples to detect adult A. frontella and the parasitoid Dacnusa dryas throughout the alfalfa growing region of Manitoba. In south central Manitoba, 100% of stems were damaged by A. frontella, and\u3e 100 adults/10 sweeps were recorded at several sites. In west central Manitoba, no plants were damaged and \u3c 10 adults/10 sweeps were observed. We believe this region to be near the western edge of A. frontella distribution. The most important introduced parasitoid of A. frontella, D. dryas, was not detected which suggests that D. dryas has not invaded Manitoba

Distribution of an Exotic Pest, \u3ci\u3eAgromyza Frontella\u3c/i\u3e (Diptera: Agromyzidae), in Manitoba, Canada.

Agromyza frontella is an exotic alfalfa pest from Europe that was first detected in North America in 1968 and has since spread westward into Ontario and the north central United States. Informal surveys had detected A. frontella in Manitoba, but its distribution throughout this province was unknown. In 1998 we collected alfalfa stems to detect plant damage and sweep samples to detect adult A. frontella and the parasitoid Dacnusa dryas throughout the alfalfa growing region of Manitoba. In south central Manitoba, 100% of stems were damaged by A. frontella, and\u3e 100 adults/10 sweeps were recorded at several sites. In west central Manitoba, no plants were damaged and \u3c 10 adults/10 sweeps were observed. We believe this region to be near the western edge of A. frontella distribution. The most important introduced parasitoid of A. frontella, D. dryas, was not detected which suggests that D. dryas has not invaded Manitoba

Environmental Consequences of Invasive Species: Greenhouse Gas Emissions of Insecticide Use and the Role of Biological Control in Reducing Emissions

Greenhouse gas emissions associated with pesticide applications against invasive species constitute an environmental cost of species invasions that has remained largely unrecognized. Here we calculate greenhouse gas emissions associated with the invasion of an agricultural pest from Asia to North America. The soybean aphid, Aphis glycines, was first discovered in North America in 2000, and has led to a substantial increase in insecticide use in soybeans. We estimate that the manufacture, transport, and application of insecticides against soybean aphid results in approximately 10.6 kg of carbon dioxide (CO(2)) equivalent greenhouse gasses being emitted per hectare of soybeans treated. Given the acreage sprayed, this has led to annual emissions of between 6 and 40 million kg of CO(2) equivalent greenhouse gasses in the United States since the invasion of soybean aphid, depending on pest population size. Emissions would be higher were it not for the development of a threshold aphid density below which farmers are advised not to spray. Without a threshold, farmers tend to spray preemptively and the threshold allows farmers to take advantage of naturally occurring biological control of the soybean aphid, which can be substantial. We find that adoption of the soybean aphid economic threshold can lead to emission reductions of approximately 300 million kg of CO(2) equivalent greenhouse gases per year in the United States. Previous studies have documented that biological control agents such as lady beetles are capable of suppressing aphid densities below this threshold in over half of the soybean acreage in the U.S. Given the acreages involved this suggests that biological control results in annual emission reductions of over 200 million kg of CO(2) equivalents. These analyses show how interactions between invasive species and organisms that suppress them can interact to affect greenhouse gas emissions

Choosing Organic Pesticides over Synthetic Pesticides May Not Effectively Mitigate Environmental Risk in Soybeans

Background: Selection of pesticides with small ecological footprints is a key factor in developing sustainable agricultural systems. Policy guiding the selection of pesticides often emphasizes natural products and organic-certified pesticides to increase sustainability, because of the prevailing public opinion that natural products are uniformly safer, and thus more environmentally friendly, than synthetic chemicals. Methodology/Principal Findings: We report the results of a study examining the environmental impact of several new synthetic and certified organic insecticides under consideration as reduced-risk insecticides for soybean aphid (Aphis glycines) control, using established and novel methodologies to directly quantify pesticide impact in terms of biocontrol services. We found that in addition to reduced efficacy against aphids compared to novel synthetic insecticides, organic approved insecticides had a similar or even greater negative impact on several natural enemy species in lab studies, were more detrimental to biological control organisms in field experiments, and had higher Environmental Impact Quotients at field use rates. Conclusions/Significance: These data bring into caution the widely held assumption that organic pesticides are more environmentally benign than synthetic ones. All pesticides must be evaluated using an empirically-based risk assessment

Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors

Insertion of a transgene into a defined genomic locus in human embryonic stem cells (hESCs) is crucial in preventing random integration-induced insertional mutagenesis, and can possibly enable persistent transgene expression during hESC expansion and in their differentiated progenies. Here, we employed homologous recombination in hESCs to introduce heterospecific loxP sites into the AAVS1 locus, a site with an open chromatin structure that allows averting transgene silencing phenomena. We then performed Cre recombinase mediated cassette exchange using baculoviral vectors to insert a transgene into the modified AAVS1 locus. Targeting efficiency in the master hESC line with the loxP-docking sites was up to 100%. Expression of the inserted transgene lasted for at least 20 passages during hESC expansion and was retained in differentiated cells derived from the genetically modified hESCs. Thus, this study demonstrates the feasibility of genetic manipulation at the AAVS1 locus with homologous recombination and using viral transduction in hESCs to facilitate recombinase-mediated cassette exchange. The method developed will be useful for repeated gene targeting at a defined locus of the hESC genome

Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)

When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes

The effect of differential rotation on Jupiter's low-degree even gravity moments

The close-by orbits of the ongoing Juno mission allow measuring with unprecedented accuracy Jupiter's low-degree even gravity moments J(2), J(4), J(6), and J(8). These can be used to better determine Jupiter's internal density profile and constrain its core mass. Yet the largest unknown on these gravity moments comes from the effect of differential rotation, which gives a degree of freedom unaccounted for by internal structure models. Here considering a wide range of possible internal flow structures and dynamical considerations, we provide upper bounds to the effect of dynamics (differential rotation) on the low-degree gravity moments. In light of the recent Juno gravity measurements and their small uncertainties, this allows differentiating between the various models suggested for Jupiter's internal structure.Israeli Ministry of Science; Minerva foundation; Federal German Ministry of Education and Research; Helen Kimmel Center for Planetary Science at the Weizmann Institute of Science; CNES; BSF; NSF; Juno project6 month embargo; Published Online: 19 June 2017This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Sex Determination in Honeybees: Two Separate Mechanisms Induce and Maintain the Female Pathway

Sex determination in honeybees is realized by the csd and the fem gene that establish and maintain, throughout development, sexual fates via the control of alternative splicing

Metabolomics of aging assessed in individual parasitoid wasps

Metabolomics studies of low-biomass organisms, such as small insects, have previously relied on the pooling of biological samples to overcome detection limits, particularly using NMR. We show that the differentiation of metabolite profiles of individual 1 mg parasitoid wasps of different ages is possible when using a modified sample preparation and a combination of untargeted NMR and LC-MS based metabolomics. Changes were observed between newly emerged and older wasps in glycerolipids, amino acids and circulatory sugars. This advance in chemical profiling has important implications for the study of the behaviour and ecology of parasitoids and many other species of small organisms because predictions and observations are typically made at the level of the individual. Thus, the metabolomic state of low-biomass individuals can now be related to their behaviour and ecological performance. We discuss specifically the utility of age-related metabolomic profiling but our new approach can be applied to a wide range of biological research