2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.

Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology (2021) 166:3567–3579. https://doi.org/10.1007/s00705-021-05266-wIn March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.This work was supported in part through Laulima Government Solutions, LLC prime contract with the US National Institute of Allergy and Infectious Diseases (NIAID) under Contract No. HHSN272201800013C. J.H.K. performed this work as an employee of Tunnell Government Services (TGS), a subcontractor of Laulima Government Solutions, LLC under Contract No. HHSN272201800013C. This work was also supported in part with federal funds from the National Cancer Institute (NCI), National Institutes of Health (NIH), under Contract No. 75N91019D00024, Task Order No. 75N91019F00130 to I.C., who was supported by the Clinical Monitoring Research Program Directorate, Frederick National Lab for Cancer Research. This work was also funded in part by Contract No. HSHQDC-15-C-00064 awarded by DHS S&T for the management and operation of The National Biodefense Analysis and Countermeasures Center, a federally funded research and development center operated by the Battelle National Biodefense Institute (V.W.); and NIH contract HHSN272201000040I/HHSN27200004/D04 and grant R24AI120942 (N.V., R.B.T.). S.S. acknowledges partial support from the Special Research Initiative of Mississippi Agricultural and Forestry Experiment Station (MAFES), Mississippi State University, and the National Institute of Food and Agriculture, US Department of Agriculture, Hatch Project 1021494. Part of this work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001030), the UK Medical Research Council (FC001030), and the Wellcome Trust (FC001030).S

Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria: kingdom Orthornavirae: phylum Negarnaviricota)

55 Pág.In April 2023, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by one new family, 14 new genera, and 140 new species. Two genera and 538 species were renamed. One species was moved, and four were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.This work was supported in part through the Laulima Government Solutions, LLC, prime contract with the U.S. National Institute of Allergy and Infec tious Diseases (NIAID) under Contract No. HHSN272201800013C. J.H.K. performed this work as an employee of Tunnell Government Services (TGS), a subcontractor of Laulima Government Solutions, LLC, under Contract No. HHSN272201800013C. U.J.B. was supported by the Division of Intramural Resarch, NIAID. This work was also funded in part by Contract No. HSHQDC15-C-00064 awarded by DHS S and T for the management and operation of The National Biodefense Analysis and Countermeasures Centre, a federally funded research and development centre operated by the Battelle National Biodefense Institute (V.W.); and NIH contract HHSN272201000040I/HHSN27200004/D04 and grant R24AI120942 (N.V., R.B.T.). S.S. acknowl edges support from the Mississippi Agricultural and Forestry Experiment Station (MAFES), USDA-ARS project 58-6066-9-033 and the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project, under Accession Number 1021494. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of the Army, the U.S. Department of Defence, the U.S. Department of Health and Human Services, including the Centres for Disease Control and Prevention, the U.S. Department of Homeland Security (DHS) Science and Technology Directorate (S and T), or of the institutions and companies affiliated with the authors. In no event shall any of these entities have any responsibility or liability for any use, misuse, inability to use, or reliance upon the information contained herein. The U.S. departments do not endorse any products or commercial services mentioned in this publication. The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S.Government retains a non-exclusive, paid up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for U.S. Government purposes.Peer reviewe

2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.

In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted, ten were moved and renamed, and 30 species were renamed. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV

2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.

In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV

Omecamtiv mecarbil in chronic heart failure with reduced ejection fraction, GALACTIC‐HF: baseline characteristics and comparison with contemporary clinical trials

Aims: The safety and efficacy of the novel selective cardiac myosin activator, omecamtiv mecarbil, in patients with heart failure with reduced ejection fraction (HFrEF) is tested in the Global Approach to Lowering Adverse Cardiac outcomes Through Improving Contractility in Heart Failure (GALACTIC‐HF) trial. Here we describe the baseline characteristics of participants in GALACTIC‐HF and how these compare with other contemporary trials. Methods and Results: Adults with established HFrEF, New York Heart Association functional class (NYHA) ≥ II, EF ≤35%, elevated natriuretic peptides and either current hospitalization for HF or history of hospitalization/ emergency department visit for HF within a year were randomized to either placebo or omecamtiv mecarbil (pharmacokinetic‐guided dosing: 25, 37.5 or 50 mg bid). 8256 patients [male (79%), non‐white (22%), mean age 65 years] were enrolled with a mean EF 27%, ischemic etiology in 54%, NYHA II 53% and III/IV 47%, and median NT‐proBNP 1971 pg/mL. HF therapies at baseline were among the most effectively employed in contemporary HF trials. GALACTIC‐HF randomized patients representative of recent HF registries and trials with substantial numbers of patients also having characteristics understudied in previous trials including more from North America (n = 1386), enrolled as inpatients (n = 2084), systolic blood pressure < 100 mmHg (n = 1127), estimated glomerular filtration rate < 30 mL/min/1.73 m2 (n = 528), and treated with sacubitril‐valsartan at baseline (n = 1594). Conclusions: GALACTIC‐HF enrolled a well‐treated, high‐risk population from both inpatient and outpatient settings, which will provide a definitive evaluation of the efficacy and safety of this novel therapy, as well as informing its potential future implementation

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Conception et démonstration d'un refroidisseur optique cryogénique pour les applications spatiales

Optical refrigeration in solids or laser cooling in solids is a breakthrough technology, reaching cryogenic temperatures (below 120 K –150 K) by the illumination of rare-earths ions doped crystals with an infrared laser at the adapted wavelength. The excitation of such a crystal near the gap wavelengths between fundamental and excited ionic states allows to predominantly stimulate the Anti-Stokes emission process, phenomenon by which the crystal re-emits more light than it has absorbed, hence cooling it down. Cryocoolers based on this revolutionary principle have the potential to ease or render possible a number of instrumental applications where vibrations and the bulkiness of traditional mechanical cryocoolers (ex. : Stirling/Pulse-Tube, Joule-Thomson, Turbo-Brayton) are stumbling blocks. The historical major targeted application is the cooling of detectors onboard Earth observation satellites, especially in the most sensitive instruments where vibrations are detrimental for performances, or onboard small satellites such as Microsatellites or even Nanosatellites, disposing of limited payloads and strong associated constraints. This thesis inserts in the frame of a French collaboration between Air Liquide Advanced Technologies (Sassenage), European leader of space cryogenics and Institut Néel, CNRS (Grenoble). The first goal of my thesis was to demonstrate the operation of a Laser Cryocooler prototype aimed at space applications for the first time in Europe. In three years, we successfully designed, developed and operated a Laser Cooler laboratory prototype capable of reaching cryogenics temperatures, thus advancing this technology to TRL 3 maturity. The 7.5% Ytterbium doped YLiF4 cooling crystal lent by the University of Pisa for our experiment is capable of cooling down to temperatures close to 130 K (-153 °C) with 10 W of absorbed laser power in about 30 minutes. In our system, the laser is supplied to the cooling crystal through an optical fiber in order to already take into account some of the constraints in a satellite application, making it a world premiere. The second goal of my thesis was to study the feasibility and suitability of Laser Cryocoolers for future Earth observation satellites. Based on the power architecture of small low earth orbit infrared observation satellites, we compared the balance of a solution with a Laser Cryocooler versus a Pulse-Tube based solution in terms of size, weight and power at the level of the whole satellite. We show that a Laser Cryocooler is a compact system which allows for internal volume and mass savings in the payload part, on top of its other advantages. This work opens the way to a new generation of cryocoolers for future space applications: Laser Cryocoolers are miniaturized, vibration-less and features reduced thermal losses due to the optical and contact-free nature of the technology.Le refroidissement optique dans les solides ou refroidissement laser dans les solides est une technologie de rupture, atteignant des températures cryogéniques (inférieures à 120 K –150 K) par l’éclairement de cristaux dopés aux ions de terre-rares avec un laser infrarouge à la longueur d’onde adaptée. L’excitation d’un tel cristal aux longueurs d’ondes proches du gap entre états fondamentaux et états excités ioniques permet de stimuler préférentiellement le processus d’émission Anti-Stokes, phénomène par lequel le cristal ré-émet plus de puissance lumineuse que ce qu’il a absorbé, provoquant ainsi son refroidissement. Les cryo-refroidisseurs basés sur ce principe révolutionnaire ont le potentiel de faciliter ou rendre possible nombre d’applications ou les vibrations et l’encombrement des cryo-refroidisseurs mécaniques traditionnels (ex. : Stirling/Pulse-Tube, Joule-Thomson, Turbo-Brayton) sont des points bloquants. L’application majeure historiquement visée est le refroidissement des détecteurs à bord des satellites d’observation de la terre, particulièrement dans les instruments les plus sensibles pour lesquels les vibrations sont préjudiciables aux performances, ou encore à bord de petits satellites du type Microsatellite ou même Nanosatellite, disposant d’une partie charge utile réduite et de fortes contraintes associées. Cette thèse s’inscrit dans le cadre d’une collaboration française entre Air Liquide Advanced Technologies (Sassenage), leader Européen de la cryogénie spatiale et l’Institut Néel, CNRS (Grenoble). Le premier but de ma thèse a été de démontrer le fonctionnement d’un prototype de Cryo-refroidisseur Laser en vue d’applications spatiales pour la première fois en Europe. En trois ans, nous avons conçu, développé et fait marcher un prototype de refroidisseurLaser capable d’atteindre des températures cryogéniques, avançant ainsi la maturité de cette technologie au niveau TRL 3. Le cristal refroidisseur de YLiF4 dopé à 7.5% d’Ytterbium prêté par l’Université de Pise pour notre expérience est capable de refroidir à des températures proches de 130 K (-153 °C) avec 10 W de puissance laser absorbée en environ 30 minutes. Dans notre système, le laser est acheminé vers le crystal refroidisseur à travers une fibre optique dans le but de déjà prendre en compte certaines contraintes d’une application satellite, pour la première fois au monde. Le second but de ma thèse a été d’étudier la faisabilité et la pertinence d’un Cryo-refroidisseur Laser pour les futurs satellites d’observation de la Terre. Basés sur l’architecture électrique des petits satellites d’observation infrarouge, nous avons comparé le bilan d’une solution Cryo-refroidisseur Laser versus solution Pulse-Tube en termes de volume, poids et puissance au niveau du satellite complet. Nous montrons que le Cryo-refoidisseur Laser est un système compact qui permet des économies de volume interne et de masse dans la partie charge utile, en plus de ses autres avantages. Ce travail ouvre la voie vers une nouvelle génération de cryo-refroidisseurs pour les futures applications spatiales: les Cryo-refroidisseurs Laser sont miniaturisés, sans vibrations et présentent des pertes thermiques réduites de part la nature optique et sans contact de la technologie

Special Issue on Latent Variable Analysis and Signal Separation

International audienc

Special Issue on Latent Variable Analysis and Signal Separation

International audienc

Latent variable analysis and signal separation

International audienc