Oxidative stress response in pseudomonas aeruginosa

Pseudomonas aeruginosa is a Gram-negative environmental and human opportunistic pathogen highly adapted to many different environmental conditions. It can cause a wide range of serious infections, including wounds, lungs, the urinary tract, and systemic infections. The high versatility and pathogenicity of this bacterium is attributed to its genomic complexity, the expression of several virulence factors, and its intrinsic resistance to various antimicrobials. However, to thrive and establish infection, P. aeruginosa must overcome several barriers. One of these barriers is the presence of oxidizing agents (e.g., hydrogen peroxide, superoxide, and hypochlorous acid) produced by the host immune system or that are commonly used as disinfectants in a variety of different environments including hospitals. These agents damage several cellular molecules and can cause cell death. Therefore, bacteria adapt to these harsh conditions by altering gene expression and eliciting several stress responses to survive under oxidative stress. Here, we used PubMed to evaluate the current knowledge on the oxidative stress responses adopted by P. aeruginosa. We will describe the genes that are often differently expressed under oxidative stress conditions, the pathways and proteins employed to sense and respond to oxidative stress, and how these changes in gene expression influence pathogenicity and the virulence of P. aeruginosa. Understanding these responses and changes in gene expression is critical to controlling bacterial pathogenicity and developing new therapeutic agents

Land resource assessment of Northern Belize (NRI Bulletin 43, Volume 1 of 2)

The land resource assessment was undertaken from July 1989 to June 1990 by the Natural Resources Institute (NRI) of the Overseas Development Administration (ODA), assisted by Mr. A. C. S. Wright, and Dr. M. Holder of Central Farm. The main object of the survey was to produce an updated land suitability assessment of Orange Walk, Corozal, Belize and Cayo districts which had not already been surveyed by Jenkin et al. (1976) and King et al. (1989). In addition, this survey would provide a land suitability assessment for the Belize Valley based on the soil mapping of )en kin et al. Thus, together with the land resource assessments of Toledo and Stann Creek districts (King et al., 1986, 1989 respectively), a land suitability assessment for the whole of Belize is now available

Land resource assessment of Northern Belize (NRI Bulletin 43, Volume 2 of 2)

The land resource assessment was undertaken from July 1989 to June 1990 by the Natural Resources Institute (NRI) of the Overseas Development Administration (ODA), assisted by Mr. A. C. S. Wright, and Dr. M. Holder of Central Farm. The main object of the survey was to produce an updated land suitability assessment of Orange Walk, Corozal, Belize and Cayo districts which had not already been surveyed by Jenkin et al. (1976) and King et al. (1989). In addition, this survey would provide a land suitability assessment for the Belize Valley based on the soil mapping of )en kin et al. Thus, together with the land resource assessments of Toledo and Stann Creek districts (King et al., 1986, 1989 respectively), a land suitability assessment for the whole of Belize is now available

Core temperature responses to cold-water immersion recovery: A pooled-data analysis

© 2018 Human Kinetics, Inc. Purpose: To examine the effect of postexercise cold-water immersion (CWI) protocols, compared with control (CON), on the magnitude and time course of core temperature (Tc) responses. Methods: Pooled-data analyses were used to examine the Tc responses of 157 subjects from previous postexercise CWI trials in the authors’ laboratories. CWI protocols varied with different combinations of temperature, duration, immersion depth, and mode (continuous vs intermittent). Tc was examined as a double difference (ΔΔTc), calculated as the change in Tc in CWI condition minus the corresponding change in CON. The effect of CWI on ΔΔTc was assessed using separate linear mixed models across 2 time components (component 1, immersion; component 2, postintervention). Results: Intermittent CWI resulted in a mean decrease in ΔΔTc that was 0.25°C (0.10°C) (estimate [SE]) greater than continuous CWI during the immersion component (P = .02). There was a significant effect of CWI temperature during the immersion component (P = .05), where reductions in water temperature of 1°C resulted in decreases in ΔΔTc of 0.03°C (0.01°C). Similarly, the effect of CWI duration was significant during the immersion component (P = .01), where every 1 min of immersion resulted in a decrease in ΔΔTc of 0.02°C (0.01°C). The peak difference in Tc between the CWI and CON interventions during the postimmersion component occurred at 60 min postintervention. Conclusions: Variations in CWI mode, duration, and temperature may have a significant effect on the extent of change in Tc. Careful consideration should be given to determine the optimal amount of core cooling before deciding which combination of protocol factors to prescribe

Experimental and analytical study on vapor phase and liquid penetration for a high pressure diesel injector

[EN] In this study, a macroscopic characterization has been performed on a solenoid diesel injector (2200 bar-8 hole nozzle) under various non-reacting but evaporative conditions. For vapor penetration a two pass Schlieren visualization set up was selected. A high speed camera was used to record high speed images of the injection event to analyze the transient evolution of the vapor phase of the spray. The transient liquid penetration of the spray has been measured via MIE-Scattering imaging technique using a high speed camera as well. Unsteady RANS based CFD Simulations have been performed to simulate the experimental conditions and correlation results are presented. Built-in models from commercial code StarCD have been used to model spray formation which includes submodels for turbulence, nozzle flow, break-up and fuel properties. A novel CAE process using an automation and optimization tool has been used to achieve robust model settings, and the final model prediction are compared with the experimental observation for the injector characterization with respect to the non-reacting spray penetration with change in ambient and injection conditions. The model correlates well with the sensitivities for temperature and injection pressures qualitatively however improvements required to capture the density effects mainly related to the mesh orientation, fixed time step size where further analysis required.This research has been partially funded by FEDER and Spanish Ministerio de Economia y Competitividad through project TRA2015-67679-c2-1-R. Additionally Jhoan Sebastian Giraldo had a grant FPI-SUB 2 from Universitat Politecnica de Valencia.Payri, R.; Giraldo-Valderrama, JS.; Ayyapureddi, S.; Versey, Z. (2018). Experimental and analytical study on vapor phase and liquid penetration for a high pressure diesel injector. Applied Thermal Engineering. 137:721-728. https://doi.org/10.1016/j.applthermaleng.2018.03.097S72172813

Differences in Physiological Responses During Rowing and Cycle Ergometry in Elite Male Rowers

Cycle training is an important training modality of elite rowers. Cycling is the preferred alternative to on-water and ergometer rowing as it provides a reduction in compressive forces on the thoracic cage and upper extremities while still creating a local and central acclimation to endurance training. It is hypothesised, however, that there will be differences in physiological characteristics between Concept II (CII) rowing and WattBike (WB) cycling due to the principle regarding the specificity of training that elite rowers undertake. Understanding these differences will ensure more accurate training prescription when cycling. Twenty international level male rowers, [V˙O2PEAK 5.85 ± 0.58 L.min−1 (CI ± 0.26 L.min−1)] participated in two identical discontinuous incremental exercise tests on a CII rowing and WB cycle ergometer. Ergometer modalities were randomised and counterbalanced among the group and tests occurred 7 days apart. V˙O2, V˙CO2, V˙E(STPD) and HR were significantly higher for every submaximal power output on the CII compared with the WB. Maximal power output on the WB was higher than on the CII [42 ± 33 W (CI ± 14 W) p < 0.000] but V˙O2PEAK was similar between modalities. Minute ventilation at maximal exercise was 11 L.min−1 lower on CII than on WB. When data were expressed relative to modality specific V˙O2PEAK, power output was consistently lower on the CII as was submaximal V˙CO2, RER, RPE, mechanical efficiency and BLa concentration at 75% V˙O2PEAK. Across all power outputs and exercise modalities, 77% of the variance in RPE could be explained by the variance in BLa. These results demonstrate that elite rowers can attain similar V˙O2PEAK scores regardless of modality. Substantial physiological and metabolic differences are evident between CII rowing and WB cycling when power output is the independent variable with the latter being over 40 W higher. The difference in displayed power output between the ergometer modalities is attributed to differences in mechanical efficiency and a degree of power output not being accounted for on the CII ergometer. Given the lack of consistency between CII and WB power output, other physiological measures, such as HR, are better suited to prescribe WB ergometer sessions

Who Feels Disadvantaged? Reporting Discrimination in Surveys

In this chapter, we seek to shed light on the mechanisms of perceived discrimination: Who, among recent immigrants, is more likely to feel discriminated against and report it when asked in a survey? Social scientists typically define discrimination as an observable and unjust difference in the treatment of distinct groups. To personally feel discriminated against, people must be aware of the differential treatment and perceive it as unjust. We show that reporting discrimination when asked in a survey depends substantially upon individual traits, including aspects that shape whether discrimination is accepted and whether immigrants feel attached to the host society. Although respondents report less discrimination if their job situation has improved after migration, people more likely report discrimination when they originate from countries in which the national legislature represents ethnic minority groups relatively well. Earlier difficulties related to the migration process and the lack of supporting networks continue to affect the perception of unfair treatment. Moreover, we show that individuals distinguish to a surprising degree between discrimination in and outside the work environment. For instance, when they are proficient in the local language, respondents often report discrimination in the workplace but not in a public environment. This distinction between discrimination in the workplace and discrimination in public also depends strongly upon the immigrant's origin. We conclude that contemporary individual-level measures and policy recommendations merely approximate discriminatory patterns; we urge future research to consider factors that affect individual perception of discrimination

A comparison of heat acclimation by post-exercise hot water immersion and exercise in the heat.

OBJECTIVES: To compare heat acclimation adaptations after three and six days of either post-exercise hot water immersion (HWI) or exercise-heat-acclimation (EHA) in recreationally active individuals. DESIGN: Randomised, mixed model, repeated measures. METHODS: Post-exercise HWI involved a daily 40-min treadmill-run at 65% V̇O2peak in temperate conditions (19 °C, 45% RH) followed by HWI (≤40 min, 40 °C water; n = 9). Daily EHA involved a ≤60-min treadmill-run in the heat (65% V̇O2peak; 33 °C, 40% RH; n = 9), chosen to elicit a similar endogenous thermal stimulus to HWI. A thermoneutral exercise intervention (TNE, 19 °C, 45% RH; n = 9), work-matched to EHA, was also included to determine thermoregulatory adaptations to daily exercise in temperate conditions. An exercise-heat-stress-test was performed before and after three and six intervention days and involved a 40-min treadmill-run and time-to-exhaustion (TTE) at 65% V̇O2peak in the heat (33 °C, 40% RH). RESULTS: ANCOVA, using baseline values as the covariate, revealed no interaction effects but significant group effects demonstrated that compared to EHA, HWI elicited larger reductions in resting rectal temperature (Tre; p = 0.021), Tre at sweating onset (p = 0.011), and end-exercise Tre during exercise-heat-stress (-0.47 °C; p = 0.042). Despite a similar endogenous thermal stimulus to HWI, EHA elicited a modest reduction in end-exercise Tre (-0.26 °C), which was not different from TNE (-0.25 °C, p = 1.000). There were no main effects or interaction effects for end-exercise Tsk, heart rate, physiological strain index, RPE, thermal sensation, plasma volume, or TTE (all p ≥ 0.154). CONCLUSIONS: Compared with conventional short-term exercise heat acclimation, short-term post-exercise hot water immersion elicited larger thermal adaptations

A new multi-stage recession model for Proglacial Lake Humber during the retreat of the last British-Irish Ice Sheet

The single most prominent lake associated with the retreat phase of the last British-Irish Ice Sheet (BIIS) was Proglacial Lake Humber. The present research elucidates a revised regional history of Proglacial Lake Humber from its maximum elevation to its demise using a combination of landscape mapping and luminescence dating. The results of mapping multiple Lake Humber strandlines are now best described by an eight-stage recessional model. Erosional highstands of the lake can be shown to post-date the BIIS advance that deposited the Skipsea Till at around 17 ka whereas new OSL ages show that Lake Humber was nearing its demise by 15.5±0.8 ka, indicating a possible short-lived lake. Multiple lake level stands are attributed to the switching of lake outlets from the Lincolnshire Gap to the Humber Gap and to oscillations of the BIIS blocking the latter on more than one occasion and subsequently at a lower elevation with till. The horizontal or near-horizontal shorelines confirm that isostatic adjustment did not occur during the demise of Lake Humber, indicating that BIIS advances in the North Sea region and Vale of York were not only dynamic but of short duration

Mediation and Moderation of the Association Between Cynical Hostility and Systolic Blood Pressure in Low-Income Women

http://deepblue.lib.umich.edu/bitstream/2027.42/109744/1/mediationandmoderationoftheassociationbetweencynicalhostilityandsystolicbloodpressure-libre.pd